Restriction map for
pF1KSDA0704
(Linear) (Six-base) MAPSORT of: KIAA0704.seq Check: 8354 from: 1 to: 2661
KIAA0704.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AatII G_ACGT'C
Cuts at: 0 734 2661
Size: 734 1927
AccI GT'mk_AC
Cuts at: 0 90 1320 2661
Size: 90 1230 1341
Fragments arranged by size:
1341 1230 90
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 629 1102 1159 1373 2661
Size: 397 232 473 57 214 1288
Fragments arranged by size:
1288 473 397 232 214 57
AflII C'TTAA_G
Cuts at: 0 2079 2661
Size: 2079 582
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 704 2661
Size: 704 1957
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2222 2254 2661
Size: 2222 32 407
Fragments arranged by size:
2222 407 32
AlwNI CAG_nnn'CTG
Cuts at: 0 717 2661
Size: 717 1944
ApoI r'AATT_y
Cuts at: 0 570 1929 1966 2160 2661
Size: 570 1359 37 194 501
Fragments arranged by size:
1359 570 501 194 37
AvaI C'yCGr_G
Cuts at: 0 1560 2661
Size: 1560 1101
BamHI G'GATC_C
Cuts at: 0 2385 2661
Size: 2385 276
BanI G'GyrC_C
Cuts at: 0 753 1551 2599 2661
Size: 753 798 1048 62
Fragments arranged by size:
1048 798 753 62
BanII G_rGCy'C
Cuts at: 0 308 1293 1655 1703 2661
Size: 308 985 362 48 958
Fragments arranged by size:
985 958 362 308 48
BbeI G_GCGC'C
Cuts at: 0 1555 2661
Size: 1555 1106
BbsI GAAGACnn'nnnn_
Cuts at: 0 635 2661
Size: 635 2026
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 70 104 282 316 2536 2570 2661
Size: 70 34 178 34 2220 34 91
Fragments arranged by size:
2220 178 91 70 34 34 34
BfrBI ATG'CAT
Cuts at: 0 341 1780 2661
Size: 341 1439 881
Fragments arranged by size:
1439 881 341
BglII A'GATC_T
Cuts at: 0 1002 1176 2622 2661
Size: 1002 174 1446 39
Fragments arranged by size:
1446 1002 174 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 886 2661
Size: 886 1775
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1287 2661
Size: 1287 1374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1257 1708 2202 2661
Size: 1257 451 494 459
Fragments arranged by size:
1257 494 459 451
Bpu10I CC'TnA_GC
Cuts at: 0 1124 1361 2661
Size: 1124 237 1300
Fragments arranged by size:
1300 1124 237
BsaBI GATnn'nnATC
Cuts at: 0 2119 2661
Size: 2119 542
BsaHI Gr'CG_yC
Cuts at: 0 731 1552 2661
Size: 731 821 1109
Fragments arranged by size:
1109 821 731
BsaWI w'CCGG_w
Cuts at: 0 1818 2661
Size: 1818 843
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 48 78 1675 1705 2102 2132 2661
Size: 48 30 1597 30 397 30 529
Fragments arranged by size:
1597 529 397 48 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 897 1098 1101 1210 1696 2280 2661
Size: 373 524 201 3 109 486 584 381
Fragments arranged by size:
584 524 486 381 373 201 109 3
BseYI C'CCAG_C
Cuts at: 0 101 1726 2661
Size: 101 1625 935
Fragments arranged by size:
1625 935 101
BsiHKAI G_wGCw'C
Cuts at: 0 61 365 1293 1703 2105 2188 2549 2661
Size: 61 304 928 410 402 83 361 112
Fragments arranged by size:
928 410 402 361 304 112 83 61
Bsp1286I G_dGCh'C
Cuts at: 0 61 308 365 1293 1655 1703 2105 2188
Size: 61 247 57 928 362 48 402 83
Cuts at: 2188 2549 2661
Size: 361 112
Fragments arranged by size:
928 402 362 361 247 112 83 61
57 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 858 2138 2661
Size: 858 1280 523
Fragments arranged by size:
1280 858 523
BsrDI GCAATG_nn'
Cuts at: 0 597 2661
Size: 597 2064
BsrFI r'CCGG_y
Cuts at: 0 1548 1635 2661
Size: 1548 87 1026
Fragments arranged by size:
1548 1026 87
BssSI C'ACGA_G
Cuts at: 0 620 2661
Size: 620 2041
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1762 2661
Size: 1762 899
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 1638 1734 2661
Size: 675 963 96 927
Fragments arranged by size:
963 927 675 96
BstYI r'GATC_y
Cuts at: 0 781 1002 1176 2385 2622 2661
Size: 781 221 174 1209 237 39
Fragments arranged by size:
1209 781 237 221 174 39
Bsu36I CC'TnA_GG
Cuts at: 0 1594 2661
Size: 1594 1067
BtgI C'CryG_G
Cuts at: 0 1982 2661
Size: 1982 679
BtsI GCAGTG_nn'
Cuts at: 0 862 2661
Size: 862 1799
DrdI GACnn_nn'nnGTC
Cuts at: 0 1420 2650 2661
Size: 1420 1230 11
Fragments arranged by size:
1420 1230 11
EaeI y'GGCC_r
Cuts at: 0 1628 2661
Size: 1628 1033
EarI CTCTTCn'nnn_
Cuts at: 0 78 516 938 1392 2298 2661
Size: 78 438 422 454 906 363
Fragments arranged by size:
906 454 438 422 363 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1146 1886 2661
Size: 1146 740 775
Fragments arranged by size:
1146 775 740
EcoICRI GAG'CTC
Cuts at: 0 1291 1701 2661
Size: 1291 410 960
Fragments arranged by size:
1291 960 410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 629 1102 1159 1257 1373 1708 2202
Size: 397 232 473 57 98 116 335 494
Cuts at: 2202 2661
Size: 459
Fragments arranged by size:
494 473 459 397 335 232 116 98
57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1621 1708 2661
Size: 1621 87 953
Fragments arranged by size:
1621 953 87
EcoO109I rG'GnC_Cy
Cuts at: 0 870 1495 1614 2661
Size: 870 625 119 1047
Fragments arranged by size:
1047 870 625 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 210 234 1064 2661
Size: 210 24 830 1597
Fragments arranged by size:
1597 830 210 24
HaeII r_GCGC'y
Cuts at: 0 1555 2661
Size: 1555 1106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 499 531 1093 1125 1313 1345
Size: 397 32 70 32 562 32 188 32
Cuts at: 1345 1399 1431 2102 2134 2301 2333 2377 2409
Size: 54 32 671 32 167 32 44 32
Cuts at: 2409 2566 2598 2661
Size: 157 32 63
Fragments arranged by size:
671 562 397 188 167 157 70 63
54 44 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 91 477 926 2661
Size: 91 386 449 1735
Fragments arranged by size:
1735 449 386 91
HpaI GTT'AAC
Cuts at: 0 477 2661
Size: 477 2184
KasI G'GCGC_C
Cuts at: 0 1551 2661
Size: 1551 1110
MfeI C'AATT_G
Cuts at: 0 1997 2661
Size: 1997 664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2597 2661
Size: 2597 64
MscI TGG'CCA
Cuts at: 0 1630 2661
Size: 1630 1031
MslI CAynn'nnrTG
Cuts at: 0 336 686 1636 1839 2661
Size: 336 350 950 203 822
Fragments arranged by size:
950 822 350 336 203
MspA1I CmG'CkG
Cuts at: 0 101 1066 1089 1234 2661
Size: 101 965 23 145 1427
Fragments arranged by size:
1427 965 145 101 23
NaeI GCC'GGC
Cuts at: 0 1550 2661
Size: 1550 1111
NarI GG'CG_CC
Cuts at: 0 1552 2661
Size: 1552 1109
NcoI C'CATG_G
Cuts at: 0 1982 2661
Size: 1982 679
NdeI CA'TA_TG
Cuts at: 0 1419 1836 2661
Size: 1419 417 825
Fragments arranged by size:
1419 825 417
NgoMIV G'CCGG_C
Cuts at: 0 1548 2661
Size: 1548 1113
NsiI A_TGCA'T
Cuts at: 0 343 1782 2661
Size: 343 1439 879
Fragments arranged by size:
1439 879 343
PflMI CCAn_nnn'nTGG
Cuts at: 0 39 2029 2661
Size: 39 1990 632
Fragments arranged by size:
1990 632 39
PfoI T'CCnGG_A
Cuts at: 0 1528 1703 2661
Size: 1528 175 958
Fragments arranged by size:
1528 958 175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1280 1312 2661
Size: 1280 32 1349
Fragments arranged by size:
1349 1280 32
PpuMI rG'GwC_Cy
Cuts at: 0 870 1614 2661
Size: 870 744 1047
Fragments arranged by size:
1047 870 744
PsiI TTA'TAA
Cuts at: 0 2167 2661
Size: 2167 494
PstI C_TGCA'G
Cuts at: 0 869 1670 2507 2661
Size: 869 801 837 154
Fragments arranged by size:
869 837 801 154
PvuII CAG'CTG
Cuts at: 0 101 1089 2661
Size: 101 988 1572
Fragments arranged by size:
1572 988 101
SacI G_AGCT'C
Cuts at: 0 1293 1703 2661
Size: 1293 410 958
Fragments arranged by size:
1293 958 410
SalI G'TCGA_C
Cuts at: 0 89 2661
Size: 89 2572
SapI GCTCTTCn'nnn_
Cuts at: 0 78 1392 2661
Size: 78 1314 1269
Fragments arranged by size:
1314 1269 78
SexAI A'CCwGG_T
Cuts at: 0 695 2661
Size: 695 1966
SfcI C'TryA_G
Cuts at: 0 865 1666 2346 2503 2661
Size: 865 801 680 157 158
Fragments arranged by size:
865 801 680 158 157
SfoI GGC'GCC
Cuts at: 0 1553 2661
Size: 1553 1108
SmlI C'TyrA_G
Cuts at: 0 2079 2661
Size: 2079 582
StyI C'CwwG_G
Cuts at: 0 21 1490 1622 1982 2661
Size: 21 1469 132 360 679
Fragments arranged by size:
1469 679 360 132 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 157 2577 2661
Size: 157 2420 84
Fragments arranged by size:
2420 157 84
TatI w'GTAC_w
Cuts at: 0 1691 2661
Size: 1691 970
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 854 886 2661
Size: 854 32 1775
Fragments arranged by size:
1775 854 32
XbaI T'CTAG_A
Cuts at: 0 1924 2448 2661
Size: 1924 524 213
Fragments arranged by size:
1924 524 213
XmnI GAAnn'nnTTC
Cuts at: 0 1844 1991 2661
Size: 1844 147 670
Fragments arranged by size:
1844 670 147
ZraI GAC'GTC
Cuts at: 0 732 2661
Size: 732 1929
Enzymes that do cut:
AatII AccI AcuI AflII AlfI AloI AlwNI ApoI
AvaI BamHI BanI BanII BbeI BbsI BcgI BfrBI
BglII BmrI BplI BpmI Bpu10I BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII HpaI KasI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
PflMI PfoI PpiI PpuMI PsiI PstI PvuII SacI
SalI SapI SexAI SfcI SfoI SmlI StyI TaqII
TatI TstI XbaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflIII AgeI AhdI AleI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI
BciVI BclI BglI BlpI Bme1580I BmgBI BmtI BpuEI
BsaI BsaAI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII EagI EcoRI EcoRV FseI
FspI FspAI HindIII KpnI MluI NheI NotI NruI
NspI PacI PasI PciI PmeI PmlI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SanDI SbfI ScaI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XcmI XhoI XmaI