Restriction map for
pF1KA0706
(Linear) (Six-base) MAPSORT of: KIAA0706.seq Check: 4620 from: 1 to: 3309
KIAA0706.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 1865 3309
Size: 1865 1444
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1189 1762 2260 2699 3309
Size: 814 375 573 498 439 610
Fragments arranged by size:
814 610 573 498 439 375
AfeI AGC'GCT
Cuts at: 0 682 2047 3309
Size: 682 1365 1262
Fragments arranged by size:
1365 1262 682
AflIII A'CryG_T
Cuts at: 0 269 3309
Size: 269 3040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3290 3309
Size: 3290 19
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1814 1846 3309
Size: 1814 32 1463
Fragments arranged by size:
1814 1463 32
AlwNI CAG_nnn'CTG
Cuts at: 0 540 1143 1153 1947 2007 2580 2853 3309
Size: 540 603 10 794 60 573 273 456
Fragments arranged by size:
794 603 573 540 456 273 60 10
ApaI G_GGCC'C
Cuts at: 0 526 780 2163 2505 2673 3227 3309
Size: 526 254 1383 342 168 554 82
Fragments arranged by size:
1383 554 526 342 254 168 82
ApoI r'AATT_y
Cuts at: 0 933 3309
Size: 933 2376
AvaI C'yCGr_G
Cuts at: 0 779 2399 2474 3119 3309
Size: 779 1620 75 645 190
Fragments arranged by size:
1620 779 645 190 75
BanI G'GyrC_C
Cuts at: 0 7 1195 3309
Size: 7 1188 2114
Fragments arranged by size:
2114 1188 7
BanII G_rGCy'C
Cuts at: 0 526 780 979 2163 2475 2505 2673 2863
Size: 526 254 199 1184 312 30 168 190
Cuts at: 2863 3227 3309
Size: 364 82
Fragments arranged by size:
1184 526 364 312 254 199 190 168
82 30
BbeI G_GCGC'C
Cuts at: 0 1199 3309
Size: 1199 2110
BbsI GAAGACnn'nnnn_
Cuts at: 0 663 1177 1467 1495 1523 2806 3309
Size: 663 514 290 28 28 1283 503
Fragments arranged by size:
1283 663 514 503 290 28 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 236 3309
Size: 202 34 3073
Fragments arranged by size:
3073 202 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2230 2389 2479 3222 3309
Size: 2230 159 90 743 87
Fragments arranged by size:
2230 743 159 90 87
BglII A'GATC_T
Cuts at: 0 443 2261 3309
Size: 443 1818 1048
Fragments arranged by size:
1818 1048 443
BlpI GC'TnA_GC
Cuts at: 0 407 1355 3309
Size: 407 948 1954
Fragments arranged by size:
1954 948 407
Bme1580I G_kGCm'C
Cuts at: 0 359 526 780 2163 2505 2673 2912 3227
Size: 359 167 254 1383 342 168 239 315
Cuts at: 3227 3309
Size: 82
Fragments arranged by size:
1383 359 342 315 254 239 168 167
82
BmrI ACTGGGnnnn_n'
Cuts at: 0 189 2705 3309
Size: 189 2516 604
Fragments arranged by size:
2516 604 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1211 1288 1699 1915 1969 2014 2085 2406
Size: 1211 77 411 216 54 45 71 321
Cuts at: 2406 3309
Size: 903
Fragments arranged by size:
1211 903 411 321 216 77 71 54
45
Bpu10I CC'TnA_GC
Cuts at: 0 973 1136 3309
Size: 973 163 2173
Fragments arranged by size:
2173 973 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 2314 2809 3271 3309
Size: 910 1404 495 462 38
Fragments arranged by size:
1404 910 495 462 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 49 458 628 3309
Size: 49 409 170 2681
Fragments arranged by size:
2681 409 170 49
BsaAI yAC'GTr
Cuts at: 0 531 3309
Size: 531 2778
BsaHI Gr'CG_yC
Cuts at: 0 1196 1472 1562 2381 3309
Size: 1196 276 90 819 928
Fragments arranged by size:
1196 928 819 276 90
BsaWI w'CCGG_w
Cuts at: 0 1603 1637 1961 3309
Size: 1603 34 324 1348
Fragments arranged by size:
1603 1348 324 34
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 88 118 1652 1682 1942 1972 2755 2785
Size: 88 30 1534 30 260 30 783 30
Cuts at: 2785 3309
Size: 524
Fragments arranged by size:
1534 783 524 260 88 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 759 1012 1390 2067 2440 2479 2713 2800
Size: 759 253 378 677 373 39 234 87
Cuts at: 2800 3034 3309
Size: 234 275
Fragments arranged by size:
759 677 378 373 275 253 234 234
87 39
BseYI C'CCAG_C
Cuts at: 0 301 359 748 1221 1649 2140 2186 2219
Size: 301 58 389 473 428 491 46 33
Cuts at: 2219 2759 2895 2954 3149 3185 3204 3209 3309
Size: 540 136 59 195 36 19 5 100
Fragments arranged by size:
540 491 473 428 389 301 195 136
100 59 58 46 36 33 19 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 3119 3309
Size: 553 2566 190
Fragments arranged by size:
2566 553 190
BsiHKAI G_wGCw'C
Cuts at: 0 408 512 914 3309
Size: 408 104 402 2395
Fragments arranged by size:
2395 408 402 104
BsiWI C'GTAC_G
Cuts at: 0 527 3309
Size: 527 2782
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2372 3255 3309
Size: 2372 883 54
Fragments arranged by size:
2372 883 54
Bsp1286I G_dGCh'C
Cuts at: 0 359 408 512 526 780 914 979 2163
Size: 359 49 104 14 254 134 65 1184
Cuts at: 2163 2475 2505 2673 2863 2912 3227 3309
Size: 312 30 168 190 49 315 82
Fragments arranged by size:
1184 359 315 312 254 190 168 134
104 82 65 49 49 30 14
BspEI T'CCGG_A
Cuts at: 0 1637 3309
Size: 1637 1672
BspHI T'CATG_A
Cuts at: 0 2631 3309
Size: 2631 678
BsrBI CCG'CTC
Cuts at: 0 456 2766 2987 3006 3127 3309
Size: 456 2310 221 19 121 182
Fragments arranged by size:
2310 456 221 182 121 19
BsrDI GCAATG_nn'
Cuts at: 0 572 1056 1420 3309
Size: 572 484 364 1889
Fragments arranged by size:
1889 572 484 364
BsrFI r'CCGG_y
Cuts at: 0 3053 3309
Size: 3053 256
BstAPI GCAn_nnn'nTGC
Cuts at: 0 574 2580 2853 3309
Size: 574 2006 273 456
Fragments arranged by size:
2006 574 456 273
BstXI CCAn_nnnn'nTGG
Cuts at: 0 78 1657 1868 3309
Size: 78 1579 211 1441
Fragments arranged by size:
1579 1441 211 78
BstYI r'GATC_y
Cuts at: 0 443 1944 2180 2261 3309
Size: 443 1501 236 81 1048
Fragments arranged by size:
1501 1048 443 236 81
Bsu36I CC'TnA_GG
Cuts at: 0 771 1015 1310 1402 3017 3309
Size: 771 244 295 92 1615 292
Fragments arranged by size:
1615 771 295 292 244 92
BtgI C'CryG_G
Cuts at: 0 1319 2105 2228 2295 3309
Size: 1319 786 123 67 1014
Fragments arranged by size:
1319 1014 786 123 67
BtsI GCAGTG_nn'
Cuts at: 0 32 2151 2454 2466 3309
Size: 32 2119 303 12 843
Fragments arranged by size:
2119 843 303 32 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2112 2147 3276 3309
Size: 2112 35 1129 33
Fragments arranged by size:
2112 1129 35 33
DraIII CAC_nnn'GTG
Cuts at: 0 1503 3309
Size: 1503 1806
EaeI y'GGCC_r
Cuts at: 0 1442 1575 2321 2971 3309
Size: 1442 133 746 650 338
Fragments arranged by size:
1442 746 650 338 133
EarI CTCTTCn'nnn_
Cuts at: 0 230 382 1927 2051 3309
Size: 230 152 1545 124 1258
Fragments arranged by size:
1545 1258 230 152 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2281 2497 3309
Size: 2281 216 812
Fragments arranged by size:
2281 812 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1189 1211 1288 1699 1762 1915 1969
Size: 814 375 22 77 411 63 153 54
Cuts at: 1969 2014 2085 2260 2406 2699 3309
Size: 45 71 175 146 293 610
Fragments arranged by size:
814 610 411 375 293 175 153 146
77 71 63 54 45 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1688 2623 3309
Size: 1688 935 686
Fragments arranged by size:
1688 935 686
EcoO109I rG'GnC_Cy
Cuts at: 0 189 372 537 776 1068 1830 2159 2160
Size: 189 183 165 239 292 762 329 1
Cuts at: 2160 2316 2493 2577 2661 2670 2790 2891 3074
Size: 156 177 84 84 9 120 101 183
Cuts at: 3074 3223 3224 3309
Size: 149 1 85
Fragments arranged by size:
762 329 292 239 189 183 183 177
165 156 149 120 101 85 84 84
9 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2002 2949 3309
Size: 2002 947 360
Fragments arranged by size:
2002 947 360
FspI TGC'GCA
Cuts at: 0 2887 3309
Size: 2887 422
HaeII r_GCGC'y
Cuts at: 0 684 1199 2049 3309
Size: 684 515 850 1260
Fragments arranged by size:
1260 850 684 515
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 794 826 850 882 2032 2064
Size: 364 32 398 32 24 32 1150 32
Cuts at: 2064 2755 2787 3309
Size: 691 32 522
Fragments arranged by size:
1150 691 522 398 364 32 32 32
32 32 24
HincII GTy'rAC
Cuts at: 0 1866 3309
Size: 1866 1443
HindIII A'AGCT_T
Cuts at: 0 144 1717 3309
Size: 144 1573 1592
Fragments arranged by size:
1592 1573 144
KasI G'GCGC_C
Cuts at: 0 1195 3309
Size: 1195 2114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 3022 3033 3309
Size: 863 2159 11 276
Fragments arranged by size:
2159 863 276 11
MscI TGG'CCA
Cuts at: 0 2973 3309
Size: 2973 336
MslI CAynn'nnrTG
Cuts at: 0 625 958 2636 3309
Size: 625 333 1678 673
Fragments arranged by size:
1678 673 625 333
MspA1I CmG'CkG
Cuts at: 0 1046 1207 1734 2027 2094 2219 3213 3304
Size: 1046 161 527 293 67 125 994 91
Cuts at: 3304 3309
Size: 5
Fragments arranged by size:
1046 994 527 293 161 125 91 67
5
NaeI GCC'GGC
Cuts at: 0 3055 3309
Size: 3055 254
NarI GG'CG_CC
Cuts at: 0 1196 3309
Size: 1196 2113
NcoI C'CATG_G
Cuts at: 0 1319 2228 3309
Size: 1319 909 1081
Fragments arranged by size:
1319 1081 909
NdeI CA'TA_TG
Cuts at: 0 1704 3309
Size: 1704 1605
NgoMIV G'CCGG_C
Cuts at: 0 3053 3309
Size: 3053 256
NspI r_CATG'y
Cuts at: 0 90 789 2595 2724 3309
Size: 90 699 1806 129 585
Fragments arranged by size:
1806 699 585 129 90
PasI CC'CwG_GG
Cuts at: 0 2666 3220 3309
Size: 2666 554 89
Fragments arranged by size:
2666 554 89
PfoI T'CCnGG_A
Cuts at: 0 503 1448 3309
Size: 503 945 1861
Fragments arranged by size:
1861 945 503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 474 506 3309
Size: 474 32 2803
Fragments arranged by size:
2803 474 32
PpuMI rG'GwC_Cy
Cuts at: 0 189 372 537 1068 1830 2493 2661 2790
Size: 189 183 165 531 762 663 168 129
Cuts at: 2790 3074 3309
Size: 284 235
Fragments arranged by size:
762 663 531 284 235 189 183 168
165 129
PshAI GACnn'nnGTC
Cuts at: 0 2119 3309
Size: 2119 1190
PspOMI G'GGCC_C
Cuts at: 0 522 776 2159 2501 2669 3223 3309
Size: 522 254 1383 342 168 554 86
Fragments arranged by size:
1383 554 522 342 254 168 86
PstI C_TGCA'G
Cuts at: 0 1100 1334 1943 2204 2534 2549 2576 2849
Size: 1100 234 609 261 330 15 27 273
Cuts at: 2849 2858 3309
Size: 9 451
Fragments arranged by size:
1100 609 451 330 273 261 234 27
15 9
PvuII CAG'CTG
Cuts at: 0 1207 2094 3304 3309
Size: 1207 887 1210 5
Fragments arranged by size:
1210 1207 887 5
SalI G'TCGA_C
Cuts at: 0 1864 3309
Size: 1864 1445
SanDI GG'GwC_CC
Cuts at: 0 1830 3309
Size: 1830 1479
SexAI A'CCwGG_T
Cuts at: 0 1499 3309
Size: 1499 1810
SfcI C'TryA_G
Cuts at: 0 897 1096 1330 1939 2200 2417 2530 2545
Size: 897 199 234 609 261 217 113 15
Cuts at: 2545 2572 2845 2854 3309
Size: 27 273 9 455
Fragments arranged by size:
897 609 455 273 261 234 217 199
113 27 15 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3222 3309
Size: 3222 87
SfoI GGC'GCC
Cuts at: 0 1197 3309
Size: 1197 2112
SmaI CCC'GGG
Cuts at: 0 781 2401 3121 3309
Size: 781 1620 720 188
Fragments arranged by size:
1620 781 720 188
SmlI C'TyrA_G
Cuts at: 0 925 2329 2824 3286 3309
Size: 925 1404 495 462 23
Fragments arranged by size:
1404 925 495 462 23
SphI G_CATG'C
Cuts at: 0 90 789 2595 2724 3309
Size: 90 699 1806 129 585
Fragments arranged by size:
1806 699 585 129 90
StuI AGG'CCT
Cuts at: 0 1166 3309
Size: 1166 2143
StyI C'CwwG_G
Cuts at: 0 1319 2228 3309
Size: 1319 909 1081
Fragments arranged by size:
1319 1081 909
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1260 3309
Size: 1260 2049
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 58 90 1652 1684 3018 3050 3309
Size: 58 32 1562 32 1334 32 259
Fragments arranged by size:
1562 1334 259 58 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1555 2756 3309
Size: 1555 1201 553
Fragments arranged by size:
1555 1201 553
XmaI C'CCGG_G
Cuts at: 0 779 2399 3119 3309
Size: 779 1620 720 190
Fragments arranged by size:
1620 779 720 190
Enzymes that do cut:
AccI AcuI AfeI AflIII AlfI AloI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbsI BcgI BglI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI
BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SalI
SanDI SexAI SfcI SfiI SfoI SmaI SmlI SphI
StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BclI BfrBI BmgBI BmtI BplI BsaBI BsiEI
BsmI BspMI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI DraI DrdI EagI EcoICRI EcoRI EcoRV
FseI FspAI HpaI KpnI MfeI MluI NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PsiI
PspXI PsrI PvuI RsrII SacI SacII SapI SbfI
ScaI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI