Restriction map for
pF1KA0709
(Linear) (Six-base) MAPSORT of: KIAA0709.seq Check: 1206 from: 1 to: 4437
KIAA0709.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 50 3959 4437
Size: 50 3909 478
Fragments arranged by size:
3909 478 50
AccI GT'mk_AC
Cuts at: 0 524 4437
Size: 524 3913
Acc65I G'GTAC_C
Cuts at: 0 265 4437
Size: 265 4172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 2682 3403 4437
Size: 123 2559 721 1034
Fragments arranged by size:
2559 1034 721 123
AfeI AGC'GCT
Cuts at: 0 226 670 3362 3880 4220 4437
Size: 226 444 2692 518 340 217
Fragments arranged by size:
2692 518 444 340 226 217
AhdI GACnn_n'nnGTC
Cuts at: 0 387 4437
Size: 387 4050
AleI CACnn'nnGTG
Cuts at: 0 1951 4042 4437
Size: 1951 2091 395
Fragments arranged by size:
2091 1951 395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 824 1078 4437
Size: 824 254 3359
Fragments arranged by size:
3359 824 254
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2195 2227 2697 2729 4437
Size: 2195 32 470 32 1708
Fragments arranged by size:
2195 1708 470 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 279 1484 1941 2118 2133 3792 4437
Size: 279 1205 457 177 15 1659 645
Fragments arranged by size:
1659 1205 645 457 279 177 15
ApaI G_GGCC'C
Cuts at: 0 8 408 1459 1920 2127 2646 3746 4067
Size: 8 400 1051 461 207 519 1100 321
Cuts at: 4067 4081 4437
Size: 14 356
Fragments arranged by size:
1100 1051 519 461 400 356 321 207
14 8
ApaLI G'TGCA_C
Cuts at: 0 558 1644 2587 4437
Size: 558 1086 943 1850
Fragments arranged by size:
1850 1086 943 558
ApoI r'AATT_y
Cuts at: 0 1279 1353 4437
Size: 1279 74 3084
Fragments arranged by size:
3084 1279 74
AvaI C'yCGr_G
Cuts at: 0 64 1026 1831 2206 2639 3375 3527 4068
Size: 64 962 805 375 433 736 152 541
Cuts at: 4068 4437
Size: 369
Fragments arranged by size:
962 805 736 541 433 375 369 152
64
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 249 282 1827 1860 2429 2462 4437
Size: 249 33 1545 33 569 33 1975
Fragments arranged by size:
1975 1545 569 249 33 33 33
BamHI G'GATC_C
Cuts at: 0 2253 2927 4437
Size: 2253 674 1510
Fragments arranged by size:
2253 1510 674
BanI G'GyrC_C
Cuts at: 0 68 91 265 298 433 1397 1637 2739
Size: 68 23 174 33 135 964 240 1102
Cuts at: 2739 2822 3174 3403 3979 4136 4313 4345 4437
Size: 83 352 229 576 157 177 32 92
Fragments arranged by size:
1102 964 576 352 240 229 177 174
157 135 92 83 68 33 32 23
BanII G_rGCy'C
Cuts at: 0 8 408 1106 1412 1459 1920 2127 2456
Size: 8 400 698 306 47 461 207 329
Cuts at: 2456 2646 3188 3349 3389 3577 3746 4067 4081
Size: 190 542 161 40 188 169 321 14
Cuts at: 4081 4437
Size: 356
Fragments arranged by size:
698 542 461 400 356 329 321 306
207 190 188 169 161 47 40 14
8
BbeI G_GCGC'C
Cuts at: 0 95 1641 3983 4140 4317 4437
Size: 95 1546 2342 157 177 120
Fragments arranged by size:
2342 1546 177 157 120 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 120 1616 4437
Size: 120 1496 2821
Fragments arranged by size:
2821 1496 120
BbvCI CC'TCA_GC
Cuts at: 0 3255 4437
Size: 3255 1182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3863 3897 4437
Size: 3863 34 540
Fragments arranged by size:
3863 540 34
BglI GCCn_nnn'nGGC
Cuts at: 0 12 89 1155 1463 1847 1997 2104 2471
Size: 12 77 1066 308 384 150 107 367
Cuts at: 2471 3172 3326 3478 4437
Size: 701 154 152 959
Fragments arranged by size:
1066 959 701 384 367 308 154 152
150 107 77 12
BglII A'GATC_T
Cuts at: 0 2186 4437
Size: 2186 2251
BlpI GC'TnA_GC
Cuts at: 0 820 1528 2134 4090 4193 4437
Size: 820 708 606 1956 103 244
Fragments arranged by size:
1956 820 708 606 244 103
Bme1580I G_kGCm'C
Cuts at: 0 8 286 408 562 1459 1648 1920 1948
Size: 8 278 122 154 897 189 272 28
Cuts at: 1948 2127 2591 2646 2742 3177 3331 3382 3491
Size: 179 464 55 96 435 154 51 109
Cuts at: 3491 3746 4067 4081 4350 4437
Size: 255 321 14 269 87
Fragments arranged by size:
897 464 435 321 278 272 269 255
189 179 154 154 122 109 96 87
55 51 28 14 8
BmgBI CAC'GTC
Cuts at: 0 1577 2445 4437
Size: 1577 868 1992
Fragments arranged by size:
1992 1577 868
BmrI ACTGGGnnnn_n'
Cuts at: 0 385 878 972 983 1731 2817 3180 3203
Size: 385 493 94 11 748 1086 363 23
Cuts at: 3203 4068 4437
Size: 865 369
Fragments arranged by size:
1086 865 748 493 385 369 363 94
23 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2544 2598 3591 4437
Size: 2544 54 993 846
Fragments arranged by size:
2544 993 846 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1097 1453 1934 2301 4353 4437
Size: 184 913 356 481 367 2052 84
Fragments arranged by size:
2052 913 481 367 356 184 84
Bpu10I CC'TnA_GC
Cuts at: 0 2614 3255 3343 4437
Size: 2614 641 88 1094
Fragments arranged by size:
2614 1094 641 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 934 2453 4437
Size: 460 474 1519 1984
Fragments arranged by size:
1984 1519 474 460
BsaI GGTCTCn'nnnn_
Cuts at: 0 243 700 3295 3600 4437
Size: 243 457 2595 305 837
Fragments arranged by size:
2595 837 457 305 243
BsaAI yAC'GTr
Cuts at: 0 2534 2561 3486 3618 3666 4437
Size: 2534 27 925 132 48 771
Fragments arranged by size:
2534 925 771 132 48 27
BsaHI Gr'CG_yC
Cuts at: 0 92 104 1638 1957 3980 4137 4314 4437
Size: 92 12 1534 319 2023 157 177 123
Fragments arranged by size:
2023 1534 319 177 157 123 92 12
BsaWI w'CCGG_w
Cuts at: 0 2036 2318 4307 4437
Size: 2036 282 1989 130
Fragments arranged by size:
2036 1989 282 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1303 1333 1717 1747 2356 2386 2491 2521
Size: 1303 30 384 30 609 30 105 30
Cuts at: 2521 2716 2746 3919 3949 4437
Size: 195 30 1173 30 488
Fragments arranged by size:
1303 1173 609 488 384 195 105 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 505 853 1009 1327 1561 2167 2170
Size: 52 453 348 156 318 234 606 3
Cuts at: 2170 3313 3583 3616 3740 4437
Size: 1143 270 33 124 697
Fragments arranged by size:
1143 697 606 453 348 318 270 234
156 124 52 33 3
BseYI C'CCAG_C
Cuts at: 0 221 671 1602 1987 2015 2087 2118 2126
Size: 221 450 931 385 28 72 31 8
Cuts at: 2126 3026 3275 3354 3855 3987 4081 4233 4437
Size: 900 249 79 501 132 94 152 204
Fragments arranged by size:
931 900 501 450 385 249 221 204
152 132 94 79 72 31 28 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 585 2541 2656 3229 3276 4437
Size: 58 527 1956 115 573 47 1161
Fragments arranged by size:
1956 1161 573 527 115 58 47
BsiEI CG_ry'CG
Cuts at: 0 12 16 84 1100 2319 3285 4437
Size: 12 4 68 1016 1219 966 1152
Fragments arranged by size:
1219 1152 1016 966 68 12 4
BsiHKAI G_wGCw'C
Cuts at: 0 507 562 1648 2162 2525 2591 3389 3827
Size: 507 55 1086 514 363 66 798 438
Cuts at: 3827 3950 4437
Size: 123 487
Fragments arranged by size:
1086 798 514 507 487 438 363 123
66 55
BsmI GAATG_Cn'
Cuts at: 0 3061 4437
Size: 3061 1376
Bsp1286I G_dGCh'C
Cuts at: 0 8 286 408 507 562 1106 1412 1459
Size: 8 278 122 99 55 544 306 47
Cuts at: 1459 1648 1920 1948 2127 2162 2456 2525 2591
Size: 189 272 28 179 35 294 69 66
Cuts at: 2591 2646 2742 3177 3188 3331 3349 3382 3389
Size: 55 96 435 11 143 18 33 7
Cuts at: 3389 3491 3577 3746 3827 3950 4067 4081 4350
Size: 102 86 169 81 123 117 14 269
Cuts at: 4350 4437
Size: 87
Fragments arranged by size:
544 435 306 294 278 272 269 189
179 169 143 123 122 117 102 99
96 87 86 81 69 66 55 55
47 35 33 28 18 14 11 8
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 50 171 2897 3959 4437
Size: 50 121 2726 1062 478
Fragments arranged by size:
2726 1062 478 121 50
BsrBI CCG'CTC
Cuts at: 0 917 2067 3546 3892 4437
Size: 917 1150 1479 346 545
Fragments arranged by size:
1479 1150 917 545 346
BsrFI r'CCGG_y
Cuts at: 0 11 15 248 1768 1931 2324 4437
Size: 11 4 233 1520 163 393 2113
Fragments arranged by size:
2113 1520 393 233 163 11 4
BsrGI T'GTAC_A
Cuts at: 0 1805 2783 4437
Size: 1805 978 1654
Fragments arranged by size:
1805 1654 978
BssSI C'ACGA_G
Cuts at: 0 517 838 912 2215 4187 4437
Size: 517 321 74 1303 1972 250
Fragments arranged by size:
1972 1303 517 321 250 74
BstEII G'GTnAC_C
Cuts at: 0 379 448 625 1668 3033 4437
Size: 379 69 177 1043 1365 1404
Fragments arranged by size:
1404 1365 1043 379 177 69
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1121 3268 4437
Size: 1121 2147 1169
Fragments arranged by size:
2147 1169 1121
BstYI r'GATC_y
Cuts at: 0 813 833 1977 2186 2210 2253 2417 2426
Size: 813 20 1144 209 24 43 164 9
Cuts at: 2426 2927 4437
Size: 501 1510
Fragments arranged by size:
1510 1144 813 501 209 164 43 24
20 9
BtgI C'CryG_G
Cuts at: 0 144 308 546 594 1094 1554 1653 1842
Size: 144 164 238 48 500 460 99 189
Cuts at: 1842 1877 3306 3771 4163 4437
Size: 35 1429 465 392 274
Fragments arranged by size:
1429 500 465 460 392 274 238 189
164 144 99 48 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1041 2702 4437
Size: 1041 1661 1735
Fragments arranged by size:
1735 1661 1041
BtsI GCAGTG_nn'
Cuts at: 0 280 468 934 1173 2386 2401 3744 3825
Size: 280 188 466 239 1213 15 1343 81
Cuts at: 3825 4437
Size: 612
Fragments arranged by size:
1343 1213 612 466 280 239 188 81
15
DraIII CAC_nnn'GTG
Cuts at: 0 379 887 2166 4437
Size: 379 508 1279 2271
Fragments arranged by size:
2271 1279 508 379
EaeI y'GGCC_r
Cuts at: 0 9 13 81 292 465 1097 1862 2141
Size: 9 4 68 211 173 632 765 279
Cuts at: 2141 2168 2387 3270 4437
Size: 27 219 883 1167
Fragments arranged by size:
1167 883 765 632 279 219 211 173
68 27 9 4
EagI C'GGCC_G
Cuts at: 0 9 13 81 1097 4437
Size: 9 4 68 1016 3340
Fragments arranged by size:
3340 1016 68 9 4
EarI CTCTTCn'nnn_
Cuts at: 0 2077 4437
Size: 2077 2360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1285 2614 4437
Size: 1285 1329 1823
Fragments arranged by size:
1823 1329 1285
EcoICRI GAG'CTC
Cuts at: 0 3387 4437
Size: 3387 1050
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 184 1097 1453 1934 2301 2682 3403
Size: 123 61 913 356 481 367 381 721
Cuts at: 3403 4353 4437
Size: 950 84
Fragments arranged by size:
950 913 721 481 381 367 356 123
84 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3123 3517 4437
Size: 3123 394 920
Fragments arranged by size:
3123 920 394
EcoO109I rG'GnC_Cy
Cuts at: 0 4 404 495 1455 1755 2105 2123 3680
Size: 4 400 91 960 300 350 18 1557
Cuts at: 3680 3743 4063 4064 4078 4334 4437
Size: 63 320 1 14 256 103
Fragments arranged by size:
1557 960 400 350 320 300 256 103
91 63 18 14 4 1
EcoRI G'AATT_C
Cuts at: 0 1279 4437
Size: 1279 3158
FseI GG_CCGG'CC
Cuts at: 0 15 19 4437
Size: 15 4 4418
Fragments arranged by size:
4418 15 4
FspI TGC'GCA
Cuts at: 0 3538 4437
Size: 3538 899
HaeII r_GCGC'y
Cuts at: 0 95 228 672 1641 1861 1877 2280 2697
Size: 95 133 444 969 220 16 403 417
Cuts at: 2697 3364 3882 3983 4140 4148 4222 4317 4437
Size: 667 518 101 157 8 74 95 120
Fragments arranged by size:
969 667 518 444 417 403 220 157
133 120 101 95 95 74 16 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 1642 1674 2356 2388 4437
Size: 373 32 1237 32 682 32 2049
Fragments arranged by size:
2049 1237 682 373 32 32 32
HindIII A'AGCT_T
Cuts at: 0 4180 4437
Size: 4180 257
KasI G'GCGC_C
Cuts at: 0 91 1637 3979 4136 4313 4437
Size: 91 1546 2342 157 177 124
Fragments arranged by size:
2342 1546 177 157 124 91
KpnI G_GTAC'C
Cuts at: 0 269 4437
Size: 269 4168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1552 4079 4437
Size: 914 638 2527 358
Fragments arranged by size:
2527 914 638 358
MscI TGG'CCA
Cuts at: 0 294 467 1864 2143 2170 2389 4437
Size: 294 173 1397 279 27 219 2048
Fragments arranged by size:
2048 1397 294 279 219 173 27
MslI CAynn'nnrTG
Cuts at: 0 1951 4042 4162 4402 4417 4437
Size: 1951 2091 120 240 15 20
Fragments arranged by size:
2091 1951 240 120 20 15
MspA1I CmG'CkG
Cuts at: 0 387 729 740 794 1205 1469 1527 1665
Size: 387 342 11 54 411 264 58 138
Cuts at: 1665 2130 2409 3030 3230 3275 3429 3609 3698
Size: 465 279 621 200 45 154 180 89
Cuts at: 3698 3779 4042 4103 4127 4237 4437
Size: 81 263 61 24 110 200
Fragments arranged by size:
621 465 411 387 342 279 264 263
200 200 180 154 138 110 89 81
61 58 54 45 24 11
NaeI GCC'GGC
Cuts at: 0 13 17 1933 2326 4437
Size: 13 4 1916 393 2111
Fragments arranged by size:
2111 1916 393 13 4
NarI GG'CG_CC
Cuts at: 0 92 1638 3980 4137 4314 4437
Size: 92 1546 2342 157 177 123
Fragments arranged by size:
2342 1546 177 157 123 92
NcoI C'CATG_G
Cuts at: 0 144 1554 1653 1877 3306 3771 4163 4437
Size: 144 1410 99 224 1429 465 392 274
Fragments arranged by size:
1429 1410 465 392 274 224 144 99
NgoMIV G'CCGG_C
Cuts at: 0 11 15 1931 2324 4437
Size: 11 4 1916 393 2113
Fragments arranged by size:
2113 1916 393 11 4
NspI r_CATG'y
Cuts at: 0 327 1229 2178 4089 4437
Size: 327 902 949 1911 348
Fragments arranged by size:
1911 949 902 348 327
PasI CC'CwG_GG
Cuts at: 0 97 171 318 2010 2100 2904 3786 4437
Size: 97 74 147 1692 90 804 882 651
Fragments arranged by size:
1692 882 804 651 147 97 90 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 887 1266 2118 3141 3792 4437
Size: 887 379 852 1023 651 645
Fragments arranged by size:
1023 887 852 651 645 379
PfoI T'CCnGG_A
Cuts at: 0 113 1421 2495 3818 3914 4437
Size: 113 1308 1074 1323 96 523
Fragments arranged by size:
1323 1308 1074 523 113 96
PmlI CAC'GTG
Cuts at: 0 2534 2561 4437
Size: 2534 27 1876
Fragments arranged by size:
2534 1876 27
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1891 1923 2497 2529 2986 3018 4437
Size: 1891 32 574 32 457 32 1419
Fragments arranged by size:
1891 1419 574 457 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 495 1755 4437
Size: 495 1260 2682
Fragments arranged by size:
2682 1260 495
PsiI TTA'TAA
Cuts at: 0 2722 4437
Size: 2722 1715
PspOMI G'GGCC_C
Cuts at: 0 4 404 1455 1916 2123 2642 3742 4063
Size: 4 400 1051 461 207 519 1100 321
Cuts at: 4063 4077 4437
Size: 14 360
Fragments arranged by size:
1100 1051 519 461 400 360 321 207
14 4
PstI C_TGCA'G
Cuts at: 0 155 1054 1190 1349 1754 2075 2381 3716
Size: 155 899 136 159 405 321 306 1335
Cuts at: 3716 3956 4437
Size: 240 481
Fragments arranged by size:
1335 899 481 405 321 306 240 159
155 136
PvuI CG_AT'CG
Cuts at: 0 3285 4437
Size: 3285 1152
PvuII CAG'CTG
Cuts at: 0 387 729 740 794 1205 1527 1665 2130
Size: 387 342 11 54 411 322 138 465
Cuts at: 2130 2409 3030 3230 3698 3779 4042 4103 4437
Size: 279 621 200 468 81 263 61 334
Fragments arranged by size:
621 468 465 411 387 342 334 322
279 263 200 138 81 61 54 11
SacI G_AGCT'C
Cuts at: 0 3389 4437
Size: 3389 1048
SapI GCTCTTCn'nnn_
Cuts at: 0 2077 4437
Size: 2077 2360
SbfI CC_TGCA'GG
Cuts at: 0 1190 1754 4437
Size: 1190 564 2683
Fragments arranged by size:
2683 1190 564
ScaI AGT'ACT
Cuts at: 0 1735 4437
Size: 1735 2702
SexAI A'CCwGG_T
Cuts at: 0 1247 4437
Size: 1247 3190
SfcI C'TryA_G
Cuts at: 0 151 1050 1186 1345 1623 1750 2071 2377
Size: 151 899 136 159 278 127 321 306
Cuts at: 2377 2897 3712 3952 4353 4437
Size: 520 815 240 401 84
Fragments arranged by size:
899 815 520 401 321 306 278 240
159 151 136 127 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 12 1463 2104 4437
Size: 12 1451 641 2333
Fragments arranged by size:
2333 1451 641 12
SfoI GGC'GCC
Cuts at: 0 93 1639 3981 4138 4315 4437
Size: 93 1546 2342 157 177 122
Fragments arranged by size:
2342 1546 177 157 122 93
SmaI CCC'GGG
Cuts at: 0 1833 2641 3377 4070 4437
Size: 1833 808 736 693 367
Fragments arranged by size:
1833 808 736 693 367
SmlI C'TyrA_G
Cuts at: 0 439 949 2468 4437
Size: 439 510 1519 1969
Fragments arranged by size:
1969 1519 510 439
SnaBI TAC'GTA
Cuts at: 0 3666 4437
Size: 3666 771
SphI G_CATG'C
Cuts at: 0 4089 4437
Size: 4089 348
StuI AGG'CCT
Cuts at: 0 784 1696 3508 4437
Size: 784 912 1812 929
Fragments arranged by size:
1812 929 912 784
StyI C'CwwG_G
Cuts at: 0 144 1554 1653 1877 3306 3771 4163 4437
Size: 144 1410 99 224 1429 465 392 274
Fragments arranged by size:
1429 1410 465 392 274 224 144 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 367 661 1405 1825 2373 3129 3517 4156
Size: 367 294 744 420 548 756 388 639
Cuts at: 4156 4437
Size: 281
Fragments arranged by size:
756 744 639 548 420 388 367 294
281
TatI w'GTAC_w
Cuts at: 0 1733 1805 2507 2783 4437
Size: 1733 72 702 276 1654
Fragments arranged by size:
1733 1654 702 276 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1303 1335 1573 1605 2761 2793 2806 2838
Size: 1303 32 238 32 1156 32 13 32
Cuts at: 2838 3121 3153 4437
Size: 283 32 1284
Fragments arranged by size:
1303 1284 1156 283 238 32 32 32
32 32 13
Tth111I GACn'n_nGTC
Cuts at: 0 1249 1441 4437
Size: 1249 192 2996
Fragments arranged by size:
2996 1249 192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 315 460 1134 1595 3234 3892 4161
Size: 276 39 145 674 461 1639 658 269
Cuts at: 4161 4437
Size: 276
Fragments arranged by size:
1639 674 658 461 276 276 269 145
39
XmaI C'CCGG_G
Cuts at: 0 1831 2639 3375 4068 4437
Size: 1831 808 736 693 369
Fragments arranged by size:
1831 808 736 693 369
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssSI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HindIII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PmlI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII SacI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SmaI SmlI SnaBI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AscI AseI AsiSI
AvrII BciVI BclI BfrBI BmtI BsaBI BsiWI BsmBI
BspEI BspHI BsrDI BssHII BstAPI BstBI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EcoRV FalI FspAI HincII
HpaI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PshAI PspXI PsrI RsrII SacII
SalI SanDI SgfI SgrAI SpeI SrfI SspI SwaI
XbaI XhoI XmnI ZraI