Restriction map for
pF1KSDA0712
(Linear) (Six-base) MAPSORT of: KIAA0712.seq Check: 9963 from: 1 to: 5214
KIAA0712.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3149 5214
Size: 3149 2065
AccI GT'mk_AC
Cuts at: 0 2158 2387 5214
Size: 2158 229 2827
Fragments arranged by size:
2827 2158 229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 555 829 989 1510 1592 1889 1919
Size: 49 506 274 160 521 82 297 30
Cuts at: 1919 2213 2438 2522 2824 3847 5214
Size: 294 225 84 302 1023 1367
Fragments arranged by size:
1367 1023 521 506 302 297 294 274
225 160 84 82 49 30
AhdI GACnn_n'nnGTC
Cuts at: 0 3024 5214
Size: 3024 2190
AleI CACnn'nnGTG
Cuts at: 0 3301 3562 5214
Size: 3301 261 1652
Fragments arranged by size:
3301 1652 261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 691 4838 5214
Size: 691 4147 376
Fragments arranged by size:
4147 691 376
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2847 2879 5214
Size: 2847 32 2335
Fragments arranged by size:
2847 2335 32
AlwNI CAG_nnn'CTG
Cuts at: 0 27 1881 2332 4345 4847 5214
Size: 27 1854 451 2013 502 367
Fragments arranged by size:
2013 1854 502 451 367 27
ApaI G_GGCC'C
Cuts at: 0 3951 4637 5214
Size: 3951 686 577
Fragments arranged by size:
3951 686 577
ApoI r'AATT_y
Cuts at: 0 90 214 346 853 2638 5214
Size: 90 124 132 507 1785 2576
Fragments arranged by size:
2576 1785 507 132 124 90
AvaI C'yCGr_G
Cuts at: 0 3005 3122 4085 4414 4447 5214
Size: 3005 117 963 329 33 767
Fragments arranged by size:
3005 963 767 329 117 33
AvrII C'CTAG_G
Cuts at: 0 4033 5214
Size: 4033 1181
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3787 3820 5214
Size: 3787 33 1394
Fragments arranged by size:
3787 1394 33
BamHI G'GATC_C
Cuts at: 0 2337 5214
Size: 2337 2877
BanI G'GyrC_C
Cuts at: 0 3118 3514 5214
Size: 3118 396 1700
Fragments arranged by size:
3118 1700 396
BanII G_rGCy'C
Cuts at: 0 531 1306 1567 3640 3951 4470 4637 5214
Size: 531 775 261 2073 311 519 167 577
Fragments arranged by size:
2073 775 577 531 519 311 261 167
BbsI GAAGACnn'nnnn_
Cuts at: 0 1137 2510 3538 3620 3674 3716 4564 5214
Size: 1137 1373 1028 82 54 42 848 650
Fragments arranged by size:
1373 1137 1028 848 650 82 54 42
BbvCI CC'TCA_GC
Cuts at: 0 1286 5051 5214
Size: 1286 3765 163
Fragments arranged by size:
3765 1286 163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3597 3631 5214
Size: 3597 34 1583
Fragments arranged by size:
3597 1583 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 732 1414 5214
Size: 732 682 3800
Fragments arranged by size:
3800 732 682
BclI T'GATC_A
Cuts at: 0 2484 5214
Size: 2484 2730
BfrBI ATG'CAT
Cuts at: 0 501 1753 3313 5214
Size: 501 1252 1560 1901
Fragments arranged by size:
1901 1560 1252 501
BglII A'GATC_T
Cuts at: 0 1138 2890 5214
Size: 1138 1752 2324
Fragments arranged by size:
2324 1752 1138
Bme1580I G_kGCm'C
Cuts at: 0 3123 3951 4590 4637 5102 5214
Size: 3123 828 639 47 465 112
Fragments arranged by size:
3123 828 639 465 112 47
BmgBI CAC'GTC
Cuts at: 0 231 4081 5214
Size: 231 3850 1133
Fragments arranged by size:
3850 1133 231
BmrI ACTGGGnnnn_n'
Cuts at: 0 2342 4949 5010 5214
Size: 2342 2607 61 204
Fragments arranged by size:
2607 2342 204 61
BmtI G_CTAG'C
Cuts at: 0 2089 5214
Size: 2089 3125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3491 4250 4854 5214
Size: 3491 759 604 360
Fragments arranged by size:
3491 759 604 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1408 1472 2263 2738 3222 4742 4824
Size: 463 945 64 791 475 484 1520 82
Cuts at: 4824 5214
Size: 390
Fragments arranged by size:
1520 945 791 484 475 463 390 82
64
Bpu10I CC'TnA_GC
Cuts at: 0 779 1286 3344 4577 5051 5214
Size: 779 507 2058 1233 474 163
Fragments arranged by size:
2058 1233 779 507 474 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1320 1581 1923 4204 5214
Size: 1320 261 342 2281 1010
Fragments arranged by size:
2281 1320 1010 342 261
BsaI GGTCTCn'nnnn_
Cuts at: 0 2127 3981 5214
Size: 2127 1854 1233
Fragments arranged by size:
2127 1854 1233
BsaBI GATnn'nnATC
Cuts at: 0 394 1337 5214
Size: 394 943 3877
Fragments arranged by size:
3877 943 394
BsaWI w'CCGG_w
Cuts at: 0 3692 4217 5214
Size: 3692 525 997
Fragments arranged by size:
3692 997 525
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2736 2766 5214
Size: 2736 30 2448
Fragments arranged by size:
2736 2448 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1087 1249 1265 1511 3363 3630 4554 4920
Size: 1087 162 16 246 1852 267 924 366
Cuts at: 4920 5078 5214
Size: 158 136
Fragments arranged by size:
1852 1087 924 366 267 246 162 158
136 16
BseYI C'CCAG_C
Cuts at: 0 1182 1260 2152 2789 5214
Size: 1182 78 892 637 2425
Fragments arranged by size:
2425 1182 892 637 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 824 3121 3167 3266 3721 4312 5214
Size: 111 713 2297 46 99 455 591 902
Fragments arranged by size:
2297 902 713 591 455 111 99 46
BsiEI CG_ry'CG
Cuts at: 0 2256 5214
Size: 2256 2958
BsiHKAI G_wGCw'C
Cuts at: 0 83 230 2776 3640 4236 4583 5214
Size: 83 147 2546 864 596 347 631
Fragments arranged by size:
2546 864 631 596 347 147 83
BsmI GAATG_Cn'
Cuts at: 0 133 1755 5214
Size: 133 1622 3459
Fragments arranged by size:
3459 1622 133
Bsp1286I G_dGCh'C
Cuts at: 0 83 230 531 1306 1567 2776 3123 3640
Size: 83 147 301 775 261 1209 347 517
Cuts at: 3640 3951 4236 4470 4583 4590 4637 5102 5214
Size: 311 285 234 113 7 47 465 112
Fragments arranged by size:
1209 775 517 465 347 311 301 285
261 234 147 113 112 83 47 7
BspEI T'CCGG_A
Cuts at: 0 3692 4217 5214
Size: 3692 525 997
Fragments arranged by size:
3692 997 525
BspHI T'CATG_A
Cuts at: 0 1218 5214
Size: 1218 3996
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 102 843 2910 3149 5214
Size: 102 741 2067 239 2065
Fragments arranged by size:
2067 2065 741 239 102
BsrBI CCG'CTC
Cuts at: 0 3839 5214
Size: 3839 1375
BsrDI GCAATG_nn'
Cuts at: 0 3313 5214
Size: 3313 1901
BsrGI T'GTAC_A
Cuts at: 0 4055 5214
Size: 4055 1159
BssHII G'CGCG_C
Cuts at: 0 3144 5214
Size: 3144 2070
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1159 5214
Size: 1159 4055
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3207 3303 3511 4654 4720 4794 5214
Size: 3207 96 208 1143 66 74 420
Fragments arranged by size:
3207 1143 420 208 96 74 66
BstYI r'GATC_y
Cuts at: 0 1138 1804 2337 2890 4179 4310 4341 5201
Size: 1138 666 533 553 1289 131 31 860
Cuts at: 5201 5214
Size: 13
Fragments arranged by size:
1289 1138 860 666 553 533 131 31
13
BstZ17I GTA'TAC
Cuts at: 0 2159 5214
Size: 2159 3055
Bsu36I CC'TnA_GG
Cuts at: 0 1367 2006 4537 5214
Size: 1367 639 2531 677
Fragments arranged by size:
2531 1367 677 639
BtgI C'CryG_G
Cuts at: 0 1019 1702 3011 3071 3539 4485 5214
Size: 1019 683 1309 60 468 946 729
Fragments arranged by size:
1309 1019 946 729 683 468 60
BtsI GCAGTG_nn'
Cuts at: 0 2813 3187 3249 3284 5004 5214
Size: 2813 374 62 35 1720 210
Fragments arranged by size:
2813 1720 374 210 62 35
DraIII CAC_nnn'GTG
Cuts at: 0 3507 5214
Size: 3507 1707
DrdI GACnn_nn'nnGTC
Cuts at: 0 5010 5214
Size: 5010 204
EaeI y'GGCC_r
Cuts at: 0 671 3882 4656 5214
Size: 671 3211 774 558
Fragments arranged by size:
3211 774 671 558
EarI CTCTTCn'nnn_
Cuts at: 0 473 749 1123 1529 2125 2705 5214
Size: 473 276 374 406 596 580 2509
Fragments arranged by size:
2509 596 580 473 406 374 276
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2867 3707 5214
Size: 2867 840 1507
Fragments arranged by size:
2867 1507 840
EcoICRI GAG'CTC
Cuts at: 0 3638 5214
Size: 3638 1576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 463 555 829 989 1408 1472 1510
Size: 49 414 92 274 160 419 64 38
Cuts at: 1510 1592 1889 1919 2213 2263 2438 2522 2738
Size: 82 297 30 294 50 175 84 216
Cuts at: 2738 2824 3222 3847 4742 4824 5214
Size: 86 398 625 895 82 390
Fragments arranged by size:
895 625 419 414 398 390 297 294
274 216 175 160 92 86 84 82
82 64 50 49 38 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1885 3381 3645 4809 5214
Size: 1885 1496 264 1164 405
Fragments arranged by size:
1885 1496 1164 405 264
EcoO109I rG'GnC_Cy
Cuts at: 0 832 2141 2430 2702 3126 3947 3948 4252
Size: 832 1309 289 272 424 821 1 304
Cuts at: 4252 4634 4950 5129 5156 5214
Size: 382 316 179 27 58
Fragments arranged by size:
1309 832 821 424 382 316 304 289
272 179 58 27 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 315 5214
Size: 315 4899
FspI TGC'GCA
Cuts at: 0 3164 5214
Size: 3164 2050
HaeII r_GCGC'y
Cuts at: 0 2086 4992 5214
Size: 2086 2906 222
Fragments arranged by size:
2906 2086 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 137 169 1141 1173 1330 1362 1567 1599
Size: 137 32 972 32 157 32 205 32
Cuts at: 1599 1915 1947 2188 2220 2736 2768 3010 3042
Size: 316 32 241 32 516 32 242 32
Cuts at: 3042 3610 3642 4495 4527 4657 4689 5026 5058
Size: 568 32 853 32 130 32 337 32
Cuts at: 5058 5214
Size: 156
Fragments arranged by size:
972 853 568 516 337 316 242 241
205 157 156 137 130 32 32 32
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1296 5214
Size: 1296 3918
HindIII A'AGCT_T
Cuts at: 0 4549 5214
Size: 4549 665
MfeI C'AATT_G
Cuts at: 0 518 794 5214
Size: 518 276 4420
Fragments arranged by size:
4420 518 276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 1146 2375 5214
Size: 37 1109 1229 2839
Fragments arranged by size:
2839 1229 1109 37
MscI TGG'CCA
Cuts at: 0 673 4658 5214
Size: 673 3985 556
Fragments arranged by size:
3985 673 556
MslI CAynn'nnrTG
Cuts at: 0 496 595 641 673 1223 1353 2179 3170
Size: 496 99 46 32 550 130 826 991
Cuts at: 3170 3202 3301 3562 5214
Size: 32 99 261 1652
Fragments arranged by size:
1652 991 826 550 496 261 130 99
99 46 32 32
MspA1I CmG'CkG
Cuts at: 0 36 336 656 987 1193 1285 2789 3082
Size: 36 300 320 331 206 92 1504 293
Cuts at: 3082 3132 5214
Size: 50 2082
Fragments arranged by size:
2082 1504 331 320 300 293 206 92
50 36
NcoI C'CATG_G
Cuts at: 0 1019 3011 3071 3539 4485 5214
Size: 1019 1992 60 468 946 729
Fragments arranged by size:
1992 1019 946 729 468 60
NdeI CA'TA_TG
Cuts at: 0 1860 3561 5214
Size: 1860 1701 1653
Fragments arranged by size:
1860 1701 1653
NheI G'CTAG_C
Cuts at: 0 2085 5214
Size: 2085 3129
NsiI A_TGCA'T
Cuts at: 0 503 1755 3315 5214
Size: 503 1252 1560 1899
Fragments arranged by size:
1899 1560 1252 503
NspI r_CATG'y
Cuts at: 0 505 3352 4140 4209 5214
Size: 505 2847 788 69 1005
Fragments arranged by size:
2847 1005 788 505 69
PasI CC'CwG_GG
Cuts at: 0 4647 4805 5214
Size: 4647 158 409
Fragments arranged by size:
4647 409 158
PflMI CCAn_nnn'nTGG
Cuts at: 0 276 1315 1881 4941 5214
Size: 276 1039 566 3060 273
Fragments arranged by size:
3060 1039 566 276 273
PfoI T'CCnGG_A
Cuts at: 0 302 1488 5214
Size: 302 1186 3726
Fragments arranged by size:
3726 1186 302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 762 794 5214
Size: 762 32 4420
Fragments arranged by size:
4420 762 32
PpuMI rG'GwC_Cy
Cuts at: 0 832 2141 2430 2702 3126 4950 5129 5214
Size: 832 1309 289 272 424 1824 179 85
Fragments arranged by size:
1824 1309 832 424 289 272 179 85
PshAI GACnn'nnGTC
Cuts at: 0 462 5214
Size: 462 4752
PsiI TTA'TAA
Cuts at: 0 3554 5214
Size: 3554 1660
PspOMI G'GGCC_C
Cuts at: 0 3947 4633 5214
Size: 3947 686 581
Fragments arranged by size:
3947 686 581
PspXI vC'TCGA_Gb
Cuts at: 0 3005 5214
Size: 3005 2209
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 918 950 2966 2998 5214
Size: 918 32 2016 32 2216
Fragments arranged by size:
2216 2016 918 32 32
PstI C_TGCA'G
Cuts at: 0 986 2223 2898 4570 5214
Size: 986 1237 675 1672 644
Fragments arranged by size:
1672 1237 986 675 644
PvuI CG_AT'CG
Cuts at: 0 2256 5214
Size: 2256 2958
PvuII CAG'CTG
Cuts at: 0 336 1285 2789 3082 5214
Size: 336 949 1504 293 2132
Fragments arranged by size:
2132 1504 949 336 293
SacI G_AGCT'C
Cuts at: 0 3640 5214
Size: 3640 1574
SanDI GG'GwC_CC
Cuts at: 0 2430 2702 3126 4950 5214
Size: 2430 272 424 1824 264
Fragments arranged by size:
2430 1824 424 272 264
SapI GCTCTTCn'nnn_
Cuts at: 0 1123 5214
Size: 1123 4091
SexAI A'CCwGG_T
Cuts at: 0 1886 5214
Size: 1886 3328
SfcI C'TryA_G
Cuts at: 0 432 982 1958 2219 2574 2894 3077 3894
Size: 432 550 976 261 355 320 183 817
Cuts at: 3894 4434 4566 5138 5214
Size: 540 132 572 76
Fragments arranged by size:
976 817 572 550 540 432 355 320
261 183 132 76
SmaI CCC'GGG
Cuts at: 0 3124 4087 5214
Size: 3124 963 1127
Fragments arranged by size:
3124 1127 963
SmlI C'TyrA_G
Cuts at: 0 1299 1560 1938 3005 4183 5214
Size: 1299 261 378 1067 1178 1031
Fragments arranged by size:
1299 1178 1067 1031 378 261
SphI G_CATG'C
Cuts at: 0 505 3352 4140 4209 5214
Size: 505 2847 788 69 1005
Fragments arranged by size:
2847 1005 788 505 69
StuI AGG'CCT
Cuts at: 0 894 2239 3095 5214
Size: 894 1345 856 2119
Fragments arranged by size:
2119 1345 894 856
StyI C'CwwG_G
Cuts at: 0 1019 1427 2777 3011 3071 3539 4033 4400
Size: 1019 408 1350 234 60 468 494 367
Cuts at: 4400 4485 5214
Size: 85 729
Fragments arranged by size:
1350 1019 729 494 468 408 367 234
85 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1828 4294 5214
Size: 1828 2466 920
Fragments arranged by size:
2466 1828 920
TatI w'GTAC_w
Cuts at: 0 296 4055 5214
Size: 296 3759 1159
Fragments arranged by size:
3759 1159 296
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 431 463 981 1013 3317 3349 5214
Size: 431 32 518 32 2304 32 1865
Fragments arranged by size:
2304 1865 518 431 32 32 32
XbaI T'CTAG_A
Cuts at: 0 2229 5214
Size: 2229 2985
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 714 1099 2793 3204 4666 5214
Size: 714 385 1694 411 1462 548
Fragments arranged by size:
1694 1462 714 548 411 385
XhoI C'TCGA_G
Cuts at: 0 3005 5214
Size: 3005 2209
XmaI C'CCGG_G
Cuts at: 0 3122 4085 5214
Size: 3122 963 1129
Fragments arranged by size:
3122 1129 963
XmnI GAAnn'nnTTC
Cuts at: 0 2451 3671 5214
Size: 2451 1220 1543
Fragments arranged by size:
2451 1543 1220
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlfI AloI AlwNI
ApaI ApoI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI
NspI PasI PflMI PfoI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SanDI
SapI SexAI SfcI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI ApaLI
AscI AseI AsiSI BbeI BglI BlpI BsaAI BsaHI
BsiWI BsmBI BsrFI BssSI BstBI BstEII BtgZI ClaI
CspCI DraI EagI EcoRI EcoRV FseI FspAI HpaI
KasI KpnI MluI NaeI NarI NgoMIV NotI NruI
PacI PciI PmeI PmlI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI Tth111I ZraI