Restriction map for
pF1KA0715
(Linear) (Six-base) MAPSORT of: KIAA0715.seq Check: 7598 from: 1 to: 4383
KIAA0715.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2223 2269 3832 4286 4383
Size: 2223 46 1563 454 97
Fragments arranged by size:
2223 1563 454 97 46
AatII G_ACGT'C
Cuts at: 0 1849 4383
Size: 1849 2534
AccI GT'mk_AC
Cuts at: 0 2360 3577 4383
Size: 2360 1217 806
Fragments arranged by size:
2360 1217 806
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1590 2256 4113 4383
Size: 1590 666 1857 270
Fragments arranged by size:
1857 1590 666 270
AflIII A'CryG_T
Cuts at: 0 648 1097 3359 4383
Size: 648 449 2262 1024
Fragments arranged by size:
2262 1024 648 449
AleI CACnn'nnGTG
Cuts at: 0 2212 4383
Size: 2212 2171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 459 2019 4383
Size: 459 1560 2364
Fragments arranged by size:
2364 1560 459
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 667 699 4383
Size: 667 32 3684
Fragments arranged by size:
3684 667 32
AlwNI CAG_nnn'CTG
Cuts at: 0 212 1010 1131 1512 2628 2965 2980 4383
Size: 212 798 121 381 1116 337 15 1403
Fragments arranged by size:
1403 1116 798 381 337 212 121 15
ApaI G_GGCC'C
Cuts at: 0 1470 4383
Size: 1470 2913
ApaLI G'TGCA_C
Cuts at: 0 2182 4383
Size: 2182 2201
ApoI r'AATT_y
Cuts at: 0 458 745 4383
Size: 458 287 3638
Fragments arranged by size:
3638 458 287
AseI AT'TA_AT
Cuts at: 0 2426 4383
Size: 2426 1957
AvaI C'yCGr_G
Cuts at: 0 1174 1532 1939 4383
Size: 1174 358 407 2444
Fragments arranged by size:
2444 1174 407 358
BamHI G'GATC_C
Cuts at: 0 965 1473 2723 4383
Size: 965 508 1250 1660
Fragments arranged by size:
1660 1250 965 508
BanI G'GyrC_C
Cuts at: 0 1536 1574 2257 2333 4383
Size: 1536 38 683 76 2050
Fragments arranged by size:
2050 1536 683 76 38
BanII G_rGCy'C
Cuts at: 0 1251 1470 1802 2164 2460 3658 4153 4383
Size: 1251 219 332 362 296 1198 495 230
Fragments arranged by size:
1251 1198 495 362 332 296 230 219
BbsI GAAGACnn'nnnn_
Cuts at: 0 327 796 2408 2675 3007 3748 4383
Size: 327 469 1612 267 332 741 635
Fragments arranged by size:
1612 741 635 469 332 327 267
BbvCI CC'TCA_GC
Cuts at: 0 1897 2576 4251 4383
Size: 1897 679 1675 132
Fragments arranged by size:
1897 1675 679 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 867 901 4383
Size: 867 34 3482
Fragments arranged by size:
3482 867 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3813 4383
Size: 3813 570
BclI T'GATC_A
Cuts at: 0 389 1112 4383
Size: 389 723 3271
Fragments arranged by size:
3271 723 389
BfrBI ATG'CAT
Cuts at: 0 1996 2513 3604 4383
Size: 1996 517 1091 779
Fragments arranged by size:
1996 1091 779 517
BglI GCCn_nnn'nGGC
Cuts at: 0 1963 4383
Size: 1963 2420
BlpI GC'TnA_GC
Cuts at: 0 1382 1505 4383
Size: 1382 123 2878
Fragments arranged by size:
2878 1382 123
Bme1580I G_kGCm'C
Cuts at: 0 1083 1470 1533 1541 1577 2186 2260 4383
Size: 1083 387 63 8 36 609 74 2123
Fragments arranged by size:
2123 1083 609 387 74 63 36 8
BmgBI CAC'GTC
Cuts at: 0 649 4383
Size: 649 3734
BmrI ACTGGGnnnn_n'
Cuts at: 0 1070 2669 3051 4096 4383
Size: 1070 1599 382 1045 287
Fragments arranged by size:
1599 1070 1045 382 287
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1428 3787 4383
Size: 1428 2359 596
Fragments arranged by size:
2359 1428 596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 171 1108 1411 1619 1873 2092 2650
Size: 80 91 937 303 208 254 219 558
Cuts at: 2650 2765 3082 3393 4383
Size: 115 317 311 990
Fragments arranged by size:
990 937 558 317 311 303 254 219
208 115 91 80
Bpu10I CC'TnA_GC
Cuts at: 0 1897 1950 2228 2576 3533 4239 4251 4383
Size: 1897 53 278 348 957 706 12 132
Fragments arranged by size:
1897 957 706 348 278 132 53 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1212 1812 3265 4293 4383
Size: 622 590 600 1453 1028 90
Fragments arranged by size:
1453 1028 622 600 590 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 189 1216 1387 1678 2833 4383
Size: 189 1027 171 291 1155 1550
Fragments arranged by size:
1550 1155 1027 291 189 171
BsaBI GATnn'nnATC
Cuts at: 0 970 1138 4383
Size: 970 168 3245
Fragments arranged by size:
3245 970 168
BsaHI Gr'CG_yC
Cuts at: 0 1846 4383
Size: 1846 2537
BsaWI w'CCGG_w
Cuts at: 0 2546 4383
Size: 2546 1837
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 667 697 1276 1306 1512 1542 1675 1705
Size: 667 30 579 30 206 30 133 30
Cuts at: 1705 2065 2095 3685 3715 4383
Size: 360 30 1590 30 668
Fragments arranged by size:
1590 668 667 579 360 206 133 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 556 1530 1533 1722 2113 2269 3464 3763
Size: 556 974 3 189 391 156 1195 299
Cuts at: 3763 4239 4359 4383
Size: 476 120 24
Fragments arranged by size:
1195 974 556 476 391 299 189 156
120 24 3
BseYI C'CCAG_C
Cuts at: 0 552 2115 2406 2719 4281 4383
Size: 552 1563 291 313 1562 102
Fragments arranged by size:
1563 1562 552 313 291 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 508 804 1904 2025 2268 2603 4383
Size: 198 310 296 1100 121 243 335 1780
Fragments arranged by size:
1780 1100 335 310 296 243 198 121
BsiHKAI G_wGCw'C
Cuts at: 0 1506 2186 3773 4153 4277 4339 4383
Size: 1506 680 1587 380 124 62 44
Fragments arranged by size:
1587 1506 680 380 124 62 44
BsmI GAATG_Cn'
Cuts at: 0 3015 3604 4383
Size: 3015 589 779
Fragments arranged by size:
3015 779 589
BsmBI CGTCTCn'nnnn_
Cuts at: 0 868 4383
Size: 868 3515
Bsp1286I G_dGCh'C
Cuts at: 0 1083 1251 1470 1506 1533 1541 1577 1802
Size: 1083 168 219 36 27 8 36 225
Cuts at: 1802 2164 2186 2260 2460 3658 3773 4153 4277
Size: 362 22 74 200 1198 115 380 124
Cuts at: 4277 4339 4383
Size: 62 44
Fragments arranged by size:
1198 1083 380 362 225 219 200 168
124 115 74 62 44 36 36 27
22 8
BspEI T'CCGG_A
Cuts at: 0 2546 4383
Size: 2546 1837
BspHI T'CATG_A
Cuts at: 0 386 3143 4383
Size: 386 2757 1240
Fragments arranged by size:
2757 1240 386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 220 1713 2223 2269 2402 3383 3832 4286
Size: 220 1493 510 46 133 981 449 454
Cuts at: 4286 4383
Size: 97
Fragments arranged by size:
1493 981 510 454 449 220 133 97
46
BsrBI CCG'CTC
Cuts at: 0 930 3086 4383
Size: 930 2156 1297
Fragments arranged by size:
2156 1297 930
BsrDI GCAATG_nn'
Cuts at: 0 546 1203 1736 2513 2771 3526 3737 4383
Size: 546 657 533 777 258 755 211 646
Fragments arranged by size:
777 755 657 646 546 533 258 211
BsrFI r'CCGG_y
Cuts at: 0 1556 2672 4331 4383
Size: 1556 1116 1659 52
Fragments arranged by size:
1659 1556 1116 52
BsrGI T'GTAC_A
Cuts at: 0 3812 4383
Size: 3812 571
BssSI C'ACGA_G
Cuts at: 0 3295 4383
Size: 3295 1088
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2510 4217 4383
Size: 2510 1707 166
Fragments arranged by size:
2510 1707 166
BstBI TT'CG_AA
Cuts at: 0 456 686 4383
Size: 456 230 3697
Fragments arranged by size:
3697 456 230
BstEII G'GTnAC_C
Cuts at: 0 1812 4383
Size: 1812 2571
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1164 2127 3336 4383
Size: 1164 963 1209 1047
Fragments arranged by size:
1209 1164 1047 963
BstYI r'GATC_y
Cuts at: 0 965 1277 1473 2723 4383
Size: 965 312 196 1250 1660
Fragments arranged by size:
1660 1250 965 312 196
BtgI C'CryG_G
Cuts at: 0 326 3759 4383
Size: 326 3433 624
Fragments arranged by size:
3433 624 326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1242 4339 4383
Size: 1242 3097 44
Fragments arranged by size:
3097 1242 44
BtsI GCAGTG_nn'
Cuts at: 0 43 1916 2013 4383
Size: 43 1873 97 2370
Fragments arranged by size:
2370 1873 97 43
ClaI AT'CG_AT
Cuts at: 0 2993 4383
Size: 2993 1390
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3405 3440 4383
Size: 3405 35 943
Fragments arranged by size:
3405 943 35
DraIII CAC_nnn'GTG
Cuts at: 0 4104 4217 4383
Size: 4104 113 166
Fragments arranged by size:
4104 166 113
EaeI y'GGCC_r
Cuts at: 0 365 1964 2129 3303 3326 3549 4383
Size: 365 1599 165 1174 23 223 834
Fragments arranged by size:
1599 1174 834 365 223 165 23
EarI CTCTTCn'nnn_
Cuts at: 0 173 238 1214 1418 1876 2305 2531 2867
Size: 173 65 976 204 458 429 226 336
Cuts at: 2867 3097 3385 3460 3932 4175 4383
Size: 230 288 75 472 243 208
Fragments arranged by size:
976 472 458 429 336 288 243 230
226 208 204 173 75 65
EcoICRI GAG'CTC
Cuts at: 0 4151 4383
Size: 4151 232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 171 1108 1411 1590 1619 1873 2092
Size: 80 91 937 303 179 29 254 219
Cuts at: 2092 2256 2650 2765 3082 3393 4113 4383
Size: 164 394 115 317 311 720 270
Fragments arranged by size:
937 720 394 317 311 303 270 254
219 179 164 115 91 80 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1123 2251 4383
Size: 1123 1128 2132
Fragments arranged by size:
2132 1128 1123
EcoO109I rG'GnC_Cy
Cuts at: 0 1019 1307 2112 2736 4285 4383
Size: 1019 288 805 624 1549 98
Fragments arranged by size:
1549 1019 805 624 288 98
FspI TGC'GCA
Cuts at: 0 2503 4383
Size: 2503 1880
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 509 541 585 617 1675 1707 1981 2013
Size: 509 32 44 32 1058 32 274 32
Cuts at: 2013 2065 2097 2691 2723 2975 3007 4222 4254
Size: 52 32 594 32 252 32 1215 32
Cuts at: 4254 4338 4370 4383
Size: 84 32 13
Fragments arranged by size:
1215 1058 594 509 274 252 84 52
44 32 32 32 32 32 32 32
32 32 13
HincII GTy'rAC
Cuts at: 0 2769 3372 3578 4383
Size: 2769 603 206 805
Fragments arranged by size:
2769 805 603 206
MfeI C'AATT_G
Cuts at: 0 2859 4383
Size: 2859 1524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1359 1661 4024 4383
Size: 473 886 302 2363 359
Fragments arranged by size:
2363 886 473 359 302
MscI TGG'CCA
Cuts at: 0 367 1966 2131 3305 3328 3551 4383
Size: 367 1599 165 1174 23 223 832
Fragments arranged by size:
1599 1174 832 367 223 165 23
MslI CAynn'nnrTG
Cuts at: 0 529 838 976 1348 2001 2212 2428 4383
Size: 529 309 138 372 653 211 216 1955
Fragments arranged by size:
1955 653 529 372 309 216 211 138
MspA1I CmG'CkG
Cuts at: 0 87 135 2764 4383
Size: 87 48 2629 1619
Fragments arranged by size:
2629 1619 87 48
NcoI C'CATG_G
Cuts at: 0 326 4383
Size: 326 4057
NsiI A_TGCA'T
Cuts at: 0 1998 2515 3606 4383
Size: 1998 517 1091 777
Fragments arranged by size:
1998 1091 777 517
NspI r_CATG'y
Cuts at: 0 1101 2052 3234 4177 4383
Size: 1101 951 1182 943 206
Fragments arranged by size:
1182 1101 951 943 206
PasI CC'CwG_GG
Cuts at: 0 2253 2289 4383
Size: 2253 36 2094
Fragments arranged by size:
2253 2094 36
PciI A'CATG_T
Cuts at: 0 1097 4383
Size: 1097 3286
PflMI CCAn_nnn'nTGG
Cuts at: 0 2344 2980 3732 4383
Size: 2344 636 752 651
Fragments arranged by size:
2344 752 651 636
PfoI T'CCnGG_A
Cuts at: 0 187 2069 2218 4383
Size: 187 1882 149 2165
Fragments arranged by size:
2165 1882 187 149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 667 699 4383
Size: 667 32 3684
Fragments arranged by size:
3684 667 32
PpuMI rG'GwC_Cy
Cuts at: 0 1019 1307 2736 4285 4383
Size: 1019 288 1429 1549 98
Fragments arranged by size:
1549 1429 1019 288 98
PshAI GACnn'nnGTC
Cuts at: 0 2764 4383
Size: 2764 1619
PspOMI G'GGCC_C
Cuts at: 0 1466 4383
Size: 1466 2917
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3349 3381 4383
Size: 3349 32 1002
Fragments arranged by size:
3349 1002 32
PstI C_TGCA'G
Cuts at: 0 2681 2788 3513 4383
Size: 2681 107 725 870
Fragments arranged by size:
2681 870 725 107
RsrII CG'GwC_CG
Cuts at: 0 3068 4383
Size: 3068 1315
SacI G_AGCT'C
Cuts at: 0 4153 4383
Size: 4153 230
SalI G'TCGA_C
Cuts at: 0 3576 4383
Size: 3576 807
SanDI GG'GwC_CC
Cuts at: 0 1307 4383
Size: 1307 3076
SapI GCTCTTCn'nnn_
Cuts at: 0 1876 2867 3385 3932 4383
Size: 1876 991 518 547 451
Fragments arranged by size:
1876 991 547 518 451
ScaI AGT'ACT
Cuts at: 0 4190 4383
Size: 4190 193
SfcI C'TryA_G
Cuts at: 0 995 2037 2199 2677 2784 2896 3509 4383
Size: 995 1042 162 478 107 112 613 874
Fragments arranged by size:
1042 995 874 613 478 162 112 107
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1963 4383
Size: 1963 2420
SmaI CCC'GGG
Cuts at: 0 1534 4383
Size: 1534 2849
SmlI C'TyrA_G
Cuts at: 0 637 1191 1827 3280 4308 4383
Size: 637 554 636 1453 1028 75
Fragments arranged by size:
1453 1028 637 636 554 75
SpeI A'CTAG_T
Cuts at: 0 2211 4383
Size: 2211 2172
SphI G_CATG'C
Cuts at: 0 3234 4383
Size: 3234 1149
StuI AGG'CCT
Cuts at: 0 2136 4062 4383
Size: 2136 1926 321
Fragments arranged by size:
2136 1926 321
StyI C'CwwG_G
Cuts at: 0 326 1083 1655 1709 2093 2366 4383
Size: 326 757 572 54 384 273 2017
Fragments arranged by size:
2017 757 572 384 326 273 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1240 2351 2370 2818 3056 3849 4115 4383
Size: 1240 1111 19 448 238 793 266 268
Fragments arranged by size:
1240 1111 793 448 268 266 238 19
TatI w'GTAC_w
Cuts at: 0 1283 3341 3791 3812 4188 4383
Size: 1283 2058 450 21 376 195
Fragments arranged by size:
2058 1283 450 376 195 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7 39 1512 1544 4383
Size: 7 32 1473 32 2839
Fragments arranged by size:
2839 1473 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 0 1689 2302 2380 4383
Size: 1689 613 78 2003
Fragments arranged by size:
2003 1689 613 78
XbaI T'CTAG_A
Cuts at: 0 2475 4383
Size: 2475 1908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 360 781 4383
Size: 360 421 3602
Fragments arranged by size:
3602 421 360
XmaI C'CCGG_G
Cuts at: 0 1532 4383
Size: 1532 2851
XmnI GAAnn'nnTTC
Cuts at: 0 661 2687 4383
Size: 661 2026 1696
Fragments arranged by size:
2026 1696 661
ZraI GAC'GTC
Cuts at: 0 1847 4383
Size: 1847 2536
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I HincII
MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI
PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI
PsrI PstI RsrII SacI SalI SanDI SapI ScaI
SfcI SfiI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AscI AsiSI
AvrII BaeI BbeI BglII BmtI BsaAI BsiEI BsiWI
BssHII BstZ17I Bsu36I DraI DrdI EagI EciI EcoRI
EcoRV FalI FseI FspAI HaeII HindIII HpaI KasI
KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PmeI PmlI PsiI PspXI PvuI PvuII
SacII SbfI SexAI SfoI SgfI SgrAI SnaBI SrfI
SspI SwaI XhoI