Restriction map for
pF1KA0720
(Linear) (Six-base) MAPSORT of: KIAA0720.seq Check: 1531 from: 1 to: 3186
KIAA0720.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2792 3186
Size: 2792 394
AccI GT'mk_AC
Cuts at: 0 968 3186
Size: 968 2218
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 900 1612 1873 2339 3186
Size: 900 712 261 466 847
Fragments arranged by size:
900 847 712 466 261
AfeI AGC'GCT
Cuts at: 0 1409 1726 3186
Size: 1409 317 1460
Fragments arranged by size:
1460 1409 317
AflIII A'CryG_T
Cuts at: 0 230 782 1745 2153 3186
Size: 230 552 963 408 1033
Fragments arranged by size:
1033 963 552 408 230
AhdI GACnn_n'nnGTC
Cuts at: 0 660 2100 2475 3186
Size: 660 1440 375 711
Fragments arranged by size:
1440 711 660 375
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1300 1332 3186
Size: 1300 32 1854
Fragments arranged by size:
1854 1300 32
AlwNI CAG_nnn'CTG
Cuts at: 0 108 1590 1779 1923 2268 2277 2823 2833
Size: 108 1482 189 144 345 9 546 10
Cuts at: 2833 3186
Size: 353
Fragments arranged by size:
1482 546 353 345 189 144 108 10
9
ApaI G_GGCC'C
Cuts at: 0 125 704 2697 2942 2964 3186
Size: 125 579 1993 245 22 222
Fragments arranged by size:
1993 579 245 222 125 22
ApaLI G'TGCA_C
Cuts at: 0 2237 3186
Size: 2237 949
ApoI r'AATT_y
Cuts at: 0 406 1858 3186
Size: 406 1452 1328
Fragments arranged by size:
1452 1328 406
AvaI C'yCGr_G
Cuts at: 0 814 1034 1420 2485 2690 3051 3186
Size: 814 220 386 1065 205 361 135
Fragments arranged by size:
1065 814 386 361 220 205 135
BanI G'GyrC_C
Cuts at: 0 1374 1894 2626 2836 3186
Size: 1374 520 732 210 350
Fragments arranged by size:
1374 732 520 350 210
BanII G_rGCy'C
Cuts at: 0 16 125 301 704 827 1136 2249 2697
Size: 16 109 176 403 123 309 1113 448
Cuts at: 2697 2882 2923 2942 2964 3083 3186
Size: 185 41 19 22 119 103
Fragments arranged by size:
1113 448 403 309 185 176 123 119
109 103 41 22 19 16
BbeI G_GCGC'C
Cuts at: 0 1378 1898 3186
Size: 1378 520 1288
Fragments arranged by size:
1378 1288 520
BbsI GAAGACnn'nnnn_
Cuts at: 0 1088 1675 3186
Size: 1088 587 1511
Fragments arranged by size:
1511 1088 587
BbvCI CC'TCA_GC
Cuts at: 0 1663 3186
Size: 1663 1523
BclI T'GATC_A
Cuts at: 0 1238 3186
Size: 1238 1948
BfrBI ATG'CAT
Cuts at: 0 171 3186
Size: 171 3015
BglI GCCn_nnn'nGGC
Cuts at: 0 1038 1693 3186
Size: 1038 655 1493
Fragments arranged by size:
1493 1038 655
BlpI GC'TnA_GC
Cuts at: 0 1597 3186
Size: 1597 1589
Bme1580I G_kGCm'C
Cuts at: 0 125 704 2241 2697 2942 2964 3186
Size: 125 579 1537 456 245 22 222
Fragments arranged by size:
1537 579 456 245 222 125 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 475 2508 3186
Size: 475 2033 678
Fragments arranged by size:
2033 678 475
BmtI G_CTAG'C
Cuts at: 0 1367 3186
Size: 1367 1819
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 315 3186
Size: 315 2871
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 733 822 1006 1135 1405 1948 2800
Size: 641 92 89 184 129 270 543 852
Cuts at: 2800 3186
Size: 386
Fragments arranged by size:
852 641 543 386 270 184 129 92
89
Bpu10I CC'TnA_GC
Cuts at: 0 1663 3077 3186
Size: 1663 1414 109
Fragments arranged by size:
1663 1414 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1636 2773 3186
Size: 1636 1137 413
Fragments arranged by size:
1636 1137 413
BsaI GGTCTCn'nnnn_
Cuts at: 0 2431 2518 3035 3099 3186
Size: 2431 87 517 64 87
Fragments arranged by size:
2431 517 87 87 64
BsaAI yAC'GTr
Cuts at: 0 1559 1740 3186
Size: 1559 181 1446
Fragments arranged by size:
1559 1446 181
BsaHI Gr'CG_yC
Cuts at: 0 725 1375 1805 1895 3186
Size: 725 650 430 90 1291
Fragments arranged by size:
1291 725 650 430 90
BsaWI w'CCGG_w
Cuts at: 0 320 2586 3186
Size: 320 2266 600
Fragments arranged by size:
2266 600 320
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1042 1057 1072 1087 1174 1204 1300 1330
Size: 1042 15 15 15 87 30 96 30
Cuts at: 1330 2584 2614 3186
Size: 1254 30 572
Fragments arranged by size:
1254 1042 572 96 87 30 30 30
15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 298 301 304 307 378 1081 1084
Size: 295 3 3 3 3 71 703 3
Cuts at: 1084 1504 1507 1690 1921 2299 2302 2317 2326
Size: 420 3 183 231 378 3 15 9
Cuts at: 2326 2329 2332 2335 2338 2341 2344 2803 3186
Size: 3 3 3 3 3 3 459 383
Fragments arranged by size:
703 459 420 383 378 295 231 183
71 15 9 3 3 3 3 3
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 444 510 609 691 853 940 2189 2860
Size: 444 66 99 82 162 87 1249 671
Cuts at: 2860 2935 2941 2945 3101 3125 3186
Size: 75 6 4 156 24 61
Fragments arranged by size:
1249 671 444 162 156 99 87 82
75 66 61 24 6 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 982 1186 1330 1849 3186
Size: 94 888 204 144 519 1337
Fragments arranged by size:
1337 888 519 204 144 94
BsiEI CG_ry'CG
Cuts at: 0 1787 2598 3186
Size: 1787 811 588
Fragments arranged by size:
1787 811 588
BsiHKAI G_wGCw'C
Cuts at: 0 301 331 1136 2241 2882 3186
Size: 301 30 805 1105 641 304
Fragments arranged by size:
1105 805 641 304 301 30
BsmI GAATG_Cn'
Cuts at: 0 397 3186
Size: 397 2789
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1903 2743 3186
Size: 1903 840 443
Fragments arranged by size:
1903 840 443
Bsp1286I G_dGCh'C
Cuts at: 0 16 125 301 331 704 827 1136 2241
Size: 16 109 176 30 373 123 309 1105
Cuts at: 2241 2249 2697 2882 2923 2942 2964 3083 3186
Size: 8 448 185 41 19 22 119 103
Fragments arranged by size:
1105 448 373 309 185 176 123 119
109 103 41 30 22 19 16 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1280 2792 3021 3053 3186
Size: 1280 1512 229 32 133
Fragments arranged by size:
1512 1280 229 133 32
BsrBI CCG'CTC
Cuts at: 0 1770 2600 3186
Size: 1770 830 586
Fragments arranged by size:
1770 830 586
BsrDI GCAATG_nn'
Cuts at: 0 503 3186
Size: 503 2683
BsrFI r'CCGG_y
Cuts at: 0 1377 3186
Size: 1377 1809
BssHII G'CGCG_C
Cuts at: 0 1683 3186
Size: 1683 1503
BssSI C'ACGA_G
Cuts at: 0 2062 2708 3186
Size: 2062 646 478
Fragments arranged by size:
2062 646 478
BstAPI GCAn_nnn'nTGC
Cuts at: 0 392 1923 2232 2268 3186
Size: 392 1531 309 36 918
Fragments arranged by size:
1531 918 392 309 36
BstEII G'GTnAC_C
Cuts at: 0 2007 3186
Size: 2007 1179
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2167 2454 3186
Size: 2167 287 732
Fragments arranged by size:
2167 732 287
BstYI r'GATC_y
Cuts at: 0 1995 3186
Size: 1995 1191
Bsu36I CC'TnA_GG
Cuts at: 0 1108 1939 2927 3186
Size: 1108 831 988 259
Fragments arranged by size:
1108 988 831 259
BtgI C'CryG_G
Cuts at: 0 1718 1787 2184 2432 2447 2609 2841 3186
Size: 1718 69 397 248 15 162 232 345
Fragments arranged by size:
1718 397 345 248 232 162 69 15
BtsI GCAGTG_nn'
Cuts at: 0 275 870 1174 2024 2540 3170 3186
Size: 275 595 304 850 516 630 16
Fragments arranged by size:
850 630 595 516 304 275 16
DraIII CAC_nnn'GTG
Cuts at: 0 1740 3186
Size: 1740 1446
DrdI GACnn_nn'nnGTC
Cuts at: 0 38 3186
Size: 38 3148
EaeI y'GGCC_r
Cuts at: 0 74 685 765 905 957 1610 1784 2169
Size: 74 611 80 140 52 653 174 385
Cuts at: 2169 2444 2595 3002 3186
Size: 275 151 407 184
Fragments arranged by size:
653 611 407 385 275 184 174 151
140 80 74 52
EagI C'GGCC_G
Cuts at: 0 1784 2595 3186
Size: 1784 811 591
Fragments arranged by size:
1784 811 591
EarI CTCTTCn'nnn_
Cuts at: 0 319 1018 1465 1546 1993 2276 2303 3025
Size: 319 699 447 81 447 283 27 722
Cuts at: 3025 3186
Size: 161
Fragments arranged by size:
722 699 447 447 319 283 161 81
27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1321 1579 2731 3186
Size: 1321 258 1152 455
Fragments arranged by size:
1321 1152 455 258
EcoICRI GAG'CTC
Cuts at: 0 299 1134 2880 3186
Size: 299 835 1746 306
Fragments arranged by size:
1746 835 306 299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 733 822 900 1006 1135 1405 1612
Size: 641 92 89 78 106 129 270 207
Cuts at: 1612 1873 1948 2339 2800 3186
Size: 261 75 391 461 386
Fragments arranged by size:
641 461 391 386 270 261 207 129
106 92 89 78 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 799 1107 1221 2281 3063 3186
Size: 136 663 308 114 1060 782 123
Fragments arranged by size:
1060 782 663 308 136 123 114
EcoO109I rG'GnC_Cy
Cuts at: 0 121 122 701 2094 2102 2147 2694 2960
Size: 121 1 579 1393 8 45 547 266
Cuts at: 2960 2961 3056 3186
Size: 1 95 130
Fragments arranged by size:
1393 579 547 266 130 121 95 45
8 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 411 3186
Size: 411 2775
HaeII r_GCGC'y
Cuts at: 0 953 1314 1378 1411 1728 1885 1898 3186
Size: 953 361 64 33 317 157 13 1288
Fragments arranged by size:
1288 953 361 317 157 64 33 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 646 678 1042 1074 2086 2118 2461 2493
Size: 646 32 364 32 1012 32 343 32
Cuts at: 2493 2512 2544 2584 2616 3034 3066 3186
Size: 19 32 40 32 418 32 120
Fragments arranged by size:
1012 646 418 364 343 120 40 32
32 32 32 32 32 32 19
KasI G'GCGC_C
Cuts at: 0 1374 1894 3186
Size: 1374 520 1292
Fragments arranged by size:
1374 1292 520
MluI A'CGCG_T
Cuts at: 0 1745 3186
Size: 1745 1441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 767 3186
Size: 566 201 2419
Fragments arranged by size:
2419 566 201
MscI TGG'CCA
Cuts at: 0 1612 2171 2446 3186
Size: 1612 559 275 740
Fragments arranged by size:
1612 740 559 275
MslI CAynn'nnrTG
Cuts at: 0 176 374 2452 3186
Size: 176 198 2078 734
Fragments arranged by size:
2078 734 198 176
MspA1I CmG'CkG
Cuts at: 0 19 108 691 842 857 890 920 984
Size: 19 89 583 151 15 33 30 64
Cuts at: 984 1124 1344 1626 1644 1761 1776 1920 2223
Size: 140 220 282 18 117 15 144 303
Cuts at: 2223 2232 2359 2393 2498 2763 2820 2945 2978
Size: 9 127 34 105 265 57 125 33
Cuts at: 2978 3186
Size: 208
Fragments arranged by size:
583 303 282 265 220 208 151 144
140 127 125 117 105 89 64 57
34 33 33 30 19 18 15 15
9
NarI GG'CG_CC
Cuts at: 0 1375 1895 3186
Size: 1375 520 1291
Fragments arranged by size:
1375 1291 520
NcoI C'CATG_G
Cuts at: 0 2447 2609 2841 3186
Size: 2447 162 232 345
Fragments arranged by size:
2447 345 232 162
NheI G'CTAG_C
Cuts at: 0 1363 3186
Size: 1363 1823
NsiI A_TGCA'T
Cuts at: 0 173 3186
Size: 173 3013
NspI r_CATG'y
Cuts at: 0 187 786 1515 1608 1755 3186
Size: 187 599 729 93 147 1431
Fragments arranged by size:
1431 729 599 187 147 93
PasI CC'CwG_GG
Cuts at: 0 2098 2580 3065 3186
Size: 2098 482 485 121
Fragments arranged by size:
2098 485 482 121
PciI A'CATG_T
Cuts at: 0 782 3186
Size: 782 2404
PflMI CCAn_nnn'nTGG
Cuts at: 0 2823 3186
Size: 2823 363
PfoI T'CCnGG_A
Cuts at: 0 1328 3186
Size: 1328 1858
PmlI CAC'GTG
Cuts at: 0 1559 1740 3186
Size: 1559 181 1446
Fragments arranged by size:
1559 1446 181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1300 1332 3186
Size: 1300 32 1854
Fragments arranged by size:
1854 1300 32
PpuMI rG'GwC_Cy
Cuts at: 0 2094 2102 3186
Size: 2094 8 1084
Fragments arranged by size:
2094 1084 8
PshAI GACnn'nnGTC
Cuts at: 0 2041 2498 3186
Size: 2041 457 688
Fragments arranged by size:
2041 688 457
PspOMI G'GGCC_C
Cuts at: 0 121 700 2693 2938 2960 3186
Size: 121 579 1993 245 22 226
Fragments arranged by size:
1993 579 245 226 121 22
PstI C_TGCA'G
Cuts at: 0 2273 3018 3186
Size: 2273 745 168
Fragments arranged by size:
2273 745 168
PvuII CAG'CTG
Cuts at: 0 108 691 842 890 984 1124 1344 1920
Size: 108 583 151 48 94 140 220 576
Cuts at: 1920 2223 2232 2359 2763 2820 2945 3186
Size: 303 9 127 404 57 125 241
Fragments arranged by size:
583 576 404 303 241 220 151 140
127 125 108 94 57 48 9
SacI G_AGCT'C
Cuts at: 0 301 1136 2882 3186
Size: 301 835 1746 304
Fragments arranged by size:
1746 835 304 301
SanDI GG'GwC_CC
Cuts at: 0 2094 3186
Size: 2094 1092
SapI GCTCTTCn'nnn_
Cuts at: 0 319 1465 3186
Size: 319 1146 1721
Fragments arranged by size:
1721 1146 319
SbfI CC_TGCA'GG
Cuts at: 0 3018 3186
Size: 3018 168
SfcI C'TryA_G
Cuts at: 0 573 1005 1414 2141 2269 3014 3186
Size: 573 432 409 727 128 745 172
Fragments arranged by size:
745 727 573 432 409 172 128
SfoI GGC'GCC
Cuts at: 0 1376 1896 3186
Size: 1376 520 1290
Fragments arranged by size:
1376 1290 520
SgrAI Cr'CCGG_yG
Cuts at: 0 1377 3186
Size: 1377 1809
SmaI CCC'GGG
Cuts at: 0 816 1036 1422 3186
Size: 816 220 386 1764
Fragments arranged by size:
1764 816 386 220
SmlI C'TyrA_G
Cuts at: 0 1651 2788 3186
Size: 1651 1137 398
Fragments arranged by size:
1651 1137 398
SphI G_CATG'C
Cuts at: 0 1755 3186
Size: 1755 1431
StuI AGG'CCT
Cuts at: 0 142 335 571 1213 2176 3186
Size: 142 193 236 642 963 1010
Fragments arranged by size:
1010 963 642 236 193 142
StyI C'CwwG_G
Cuts at: 0 77 1688 2447 2609 2841 3186
Size: 77 1611 759 162 232 345
Fragments arranged by size:
1611 759 345 232 162 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1589 1687 2181 2854 3186
Size: 1589 98 494 673 332
Fragments arranged by size:
1589 673 494 332 98
TatI w'GTAC_w
Cuts at: 0 1508 1976 3186
Size: 1508 468 1210
Fragments arranged by size:
1508 1210 468
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 3021 3053 3186
Size: 1174 32 1815 32 133
Fragments arranged by size:
1815 1174 133 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 267 2439 3186
Size: 267 2172 747
Fragments arranged by size:
2172 747 267
XmaI C'CCGG_G
Cuts at: 0 814 1034 1420 3186
Size: 814 220 386 1766
Fragments arranged by size:
1766 814 386 220
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AloI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BclI BfrBI BglI BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KasI MluI MmeI MscI MslI
MspA1I NarI NcoI NheI NsiI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuII SacI SanDI SapI SbfI SfcI SfoI SgrAI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AleI AlfI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BglII
BmgBI BsaBI BsiWI BspEI BspHI BsrGI BstBI BstZ17I
BtgZI ClaI CspCI DraI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI NaeI NdeI
NgoMIV NotI NruI PacI PmeI PsiI PspXI PsrI
PvuI RsrII SacII SalI ScaI SexAI SfiI SgfI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI
XmnI ZraI