Restriction map for
pF1KSDA0724
(Linear) (Six-base) MAPSORT of: KIAA0724.seq Check: 2935 from: 1 to: 2889
KIAA0724.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1140 2889
Size: 1140 1749
AatII G_ACGT'C
Cuts at: 0 1445 2889
Size: 1445 1444
AccI GT'mk_AC
Cuts at: 0 1142 1166 2889
Size: 1142 24 1723
Fragments arranged by size:
1723 1142 24
Acc65I G'GTAC_C
Cuts at: 0 210 2889
Size: 210 2679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 2422 2673 2760 2889
Size: 165 2257 251 87 129
Fragments arranged by size:
2257 251 165 129 87
AflII C'TTAA_G
Cuts at: 0 1819 2889
Size: 1819 1070
AhdI GACnn_n'nnGTC
Cuts at: 0 2248 2889
Size: 2248 641
AleI CACnn'nnGTG
Cuts at: 0 2178 2847 2889
Size: 2178 669 42
Fragments arranged by size:
2178 669 42
AlwNI CAG_nnn'CTG
Cuts at: 0 37 195 1522 1779 1860 2400 2889
Size: 37 158 1327 257 81 540 489
Fragments arranged by size:
1327 540 489 257 158 81 37
ApaI G_GGCC'C
Cuts at: 0 2348 2889
Size: 2348 541
ApoI r'AATT_y
Cuts at: 0 1262 2889
Size: 1262 1627
AvaI C'yCGr_G
Cuts at: 0 959 2889
Size: 959 1930
AvrII C'CTAG_G
Cuts at: 0 1611 2889
Size: 1611 1278
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 535 568 2889
Size: 535 33 2321
Fragments arranged by size:
2321 535 33
BanI G'GyrC_C
Cuts at: 0 210 699 1461 2169 2490 2889
Size: 210 489 762 708 321 399
Fragments arranged by size:
762 708 489 399 321 210
BanII G_rGCy'C
Cuts at: 0 764 1379 1472 1545 2348 2889
Size: 764 615 93 73 803 541
Fragments arranged by size:
803 764 615 541 93 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 594 2043 2585 2889
Size: 594 1449 542 304
Fragments arranged by size:
1449 594 542 304
BbvCI CC'TCA_GC
Cuts at: 0 455 2752 2889
Size: 455 2297 137
Fragments arranged by size:
2297 455 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2773 2807 2889
Size: 2773 34 82
Fragments arranged by size:
2773 82 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2763 2889
Size: 2763 126
BclI T'GATC_A
Cuts at: 0 1577 1974 2889
Size: 1577 397 915
Fragments arranged by size:
1577 915 397
BglI GCCn_nnn'nGGC
Cuts at: 0 388 2889
Size: 388 2501
BglII A'GATC_T
Cuts at: 0 254 1658 2889
Size: 254 1404 1231
Fragments arranged by size:
1404 1231 254
BlpI GC'TnA_GC
Cuts at: 0 1327 2074 2889
Size: 1327 747 815
Fragments arranged by size:
1327 815 747
Bme1580I G_kGCm'C
Cuts at: 0 2348 2889
Size: 2348 541
BmrI ACTGGGnnnn_n'
Cuts at: 0 543 1197 2889
Size: 543 654 1692
Fragments arranged by size:
1692 654 543
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 972 1398 2889
Size: 972 426 1491
Fragments arranged by size:
1491 972 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 288 634 663 715 1882 2629 2889
Size: 59 229 346 29 52 1167 747 260
Fragments arranged by size:
1167 747 346 260 229 59 52 29
Bpu10I CC'TnA_GC
Cuts at: 0 455 1622 1988 2698 2752 2889
Size: 455 1167 366 710 54 137
Fragments arranged by size:
1167 710 455 366 137 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 1636 2461 2889
Size: 652 984 825 428
Fragments arranged by size:
984 825 652 428
BsaI GGTCTCn'nnnn_
Cuts at: 0 165 901 1054 1426 2863 2889
Size: 165 736 153 372 1437 26
Fragments arranged by size:
1437 736 372 165 153 26
BsaAI yAC'GTr
Cuts at: 0 825 2889
Size: 825 2064
BsaBI GATnn'nnATC
Cuts at: 0 1495 2889
Size: 1495 1394
BsaHI Gr'CG_yC
Cuts at: 0 1442 2889
Size: 1442 1447
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2098 2128 2889
Size: 2098 30 761
Fragments arranged by size:
2098 761 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 538 1387 1396 1825 1891 2889
Size: 28 510 849 9 429 66 998
Fragments arranged by size:
998 849 510 429 66 28 9
BseYI C'CCAG_C
Cuts at: 0 634 643 683 858 1347 1779 2190 2889
Size: 634 9 40 175 489 432 411 699
Fragments arranged by size:
699 634 489 432 411 175 40 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 709 910 1011 1177 1528 1813 2889
Size: 73 636 201 101 166 351 285 1076
Fragments arranged by size:
1076 636 351 285 201 166 101 73
BsiEI CG_ry'CG
Cuts at: 0 2586 2889
Size: 2586 303
BsiHKAI G_wGCw'C
Cuts at: 0 243 668 764 986 1545 2889
Size: 243 425 96 222 559 1344
Fragments arranged by size:
1344 559 425 243 222 96
BsmI GAATG_Cn'
Cuts at: 0 798 2889
Size: 798 2091
Bsp1286I G_dGCh'C
Cuts at: 0 243 668 764 986 1379 1472 1545 2348
Size: 243 425 96 222 393 93 73 803
Cuts at: 2348 2889
Size: 541
Fragments arranged by size:
803 541 425 393 243 222 96 93
73
BspHI T'CATG_A
Cuts at: 0 1574 1965 1977 2265 2889
Size: 1574 391 12 288 624
Fragments arranged by size:
1574 624 391 288 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1140 1691 1835 2037 2889
Size: 1140 551 144 202 852
Fragments arranged by size:
1140 852 551 202 144
BsrBI CCG'CTC
Cuts at: 0 6 2889
Size: 6 2883
BsrFI r'CCGG_y
Cuts at: 0 1157 2889
Size: 1157 1732
BsrGI T'GTAC_A
Cuts at: 0 2201 2889
Size: 2201 688
BstAPI GCAn_nnn'nTGC
Cuts at: 0 195 888 2889
Size: 195 693 2001
Fragments arranged by size:
2001 693 195
BstXI CCAn_nnnn'nTGG
Cuts at: 0 173 229 1707 1902 2028 2889
Size: 173 56 1478 195 126 861
Fragments arranged by size:
1478 861 195 173 126 56
BstYI r'GATC_y
Cuts at: 0 218 254 1658 1739 1814 2786 2889
Size: 218 36 1404 81 75 972 103
Fragments arranged by size:
1404 972 218 103 81 75 36
BstZ17I GTA'TAC
Cuts at: 0 1143 2889
Size: 1143 1746
Bsu36I CC'TnA_GG
Cuts at: 0 521 2486 2889
Size: 521 1965 403
Fragments arranged by size:
1965 521 403
BtgI C'CryG_G
Cuts at: 0 915 1486 2027 2165 2862 2889
Size: 915 571 541 138 697 27
Fragments arranged by size:
915 697 571 541 138 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 915 1410 2889
Size: 915 495 1479
Fragments arranged by size:
1479 915 495
BtsI GCAGTG_nn'
Cuts at: 0 780 893 1096 2609 2682 2842 2889
Size: 780 113 203 1513 73 160 47
Fragments arranged by size:
1513 780 203 160 113 73 47
DrdI GACnn_nn'nnGTC
Cuts at: 0 1570 2889
Size: 1570 1319
EaeI y'GGCC_r
Cuts at: 0 415 1041 1159 1904 2889
Size: 415 626 118 745 985
Fragments arranged by size:
985 745 626 415 118
EarI CTCTTCn'nnn_
Cuts at: 0 248 319 451 769 1641 1805 1945 2305
Size: 248 71 132 318 872 164 140 360
Cuts at: 2305 2338 2889
Size: 33 551
Fragments arranged by size:
872 551 360 318 248 164 140 132
71 33
EcoICRI GAG'CTC
Cuts at: 0 762 1543 2889
Size: 762 781 1346
Fragments arranged by size:
1346 781 762
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 165 288 634 663 715 1882 2422
Size: 59 106 123 346 29 52 1167 540
Cuts at: 2422 2629 2673 2760 2889
Size: 207 44 87 129
Fragments arranged by size:
1167 540 346 207 129 123 106 87
59 52 44 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2392 2889
Size: 2392 497
EcoO109I rG'GnC_Cy
Cuts at: 0 1401 1984 2017 2295 2344 2718 2889
Size: 1401 583 33 278 49 374 171
Fragments arranged by size:
1401 583 374 278 171 49 33
EcoRV GAT'ATC
Cuts at: 0 2661 2889
Size: 2661 228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2703 2790 2889
Size: 2703 87 99
Fragments arranged by size:
2703 99 87
HaeII r_GCGC'y
Cuts at: 0 88 724 1780 2889
Size: 88 636 1056 1109
Fragments arranged by size:
1109 1056 636 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1099 1131 1872 1904 2178 2210 2234 2266
Size: 1099 32 741 32 274 32 24 32
Cuts at: 2266 2426 2458 2889
Size: 160 32 431
Fragments arranged by size:
1099 741 431 274 160 32 32 32
32 32 24
HincII GTy'rAC
Cuts at: 0 1011 1446 2889
Size: 1011 435 1443
Fragments arranged by size:
1443 1011 435
KpnI G_GTAC'C
Cuts at: 0 214 2889
Size: 214 2675
MfeI C'AATT_G
Cuts at: 0 2288 2889
Size: 2288 601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1919 1958 2176 2889
Size: 1919 39 218 713
Fragments arranged by size:
1919 713 218 39
MscI TGG'CCA
Cuts at: 0 417 1043 1906 2889
Size: 417 626 863 983
Fragments arranged by size:
983 863 626 417
MslI CAynn'nnrTG
Cuts at: 0 934 1018 1291 1525 1970 2178 2602 2847
Size: 934 84 273 234 445 208 424 245
Cuts at: 2847 2889
Size: 42
Fragments arranged by size:
934 445 424 273 245 234 208 84
42
MspA1I CmG'CkG
Cuts at: 0 185 485 609 621 647 683 693 705
Size: 185 300 124 12 26 36 10 12
Cuts at: 705 852 1176 1512 2178 2247 2881 2889
Size: 147 324 336 666 69 634 8
Fragments arranged by size:
666 634 336 324 300 185 147 124
69 36 26 12 12 10 8
NcoI C'CATG_G
Cuts at: 0 915 2165 2862 2889
Size: 915 1250 697 27
Fragments arranged by size:
1250 915 697 27
NruI TCG'CGA
Cuts at: 0 2795 2889
Size: 2795 94
NspI r_CATG'y
Cuts at: 0 1609 1764 2706 2889
Size: 1609 155 942 183
Fragments arranged by size:
1609 942 183 155
PasI CC'CwG_GG
Cuts at: 0 942 2348 2889
Size: 942 1406 541
Fragments arranged by size:
1406 942 541
PflMI CCAn_nnn'nTGG
Cuts at: 0 472 1179 1860 2103 2889
Size: 472 707 681 243 786
Fragments arranged by size:
786 707 681 472 243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1816 1848 2889
Size: 1816 32 1041
Fragments arranged by size:
1816 1041 32
PpuMI rG'GwC_Cy
Cuts at: 0 2017 2889
Size: 2017 872
PshAI GACnn'nnGTC
Cuts at: 0 1958 2889
Size: 1958 931
PspOMI G'GGCC_C
Cuts at: 0 2344 2889
Size: 2344 545
PstI C_TGCA'G
Cuts at: 0 33 200 710 752 869 1103 2045 2726
Size: 33 167 510 42 117 234 942 681
Cuts at: 2726 2889
Size: 163
Fragments arranged by size:
942 681 510 234 167 163 117 42
33
PvuII CAG'CTG
Cuts at: 0 185 621 647 683 693 1176 1512 2178
Size: 185 436 26 36 10 483 336 666
Cuts at: 2178 2247 2881 2889
Size: 69 634 8
Fragments arranged by size:
666 634 483 436 336 185 69 36
26 10 8
SacI G_AGCT'C
Cuts at: 0 764 1545 2889
Size: 764 781 1344
Fragments arranged by size:
1344 781 764
SanDI GG'GwC_CC
Cuts at: 0 2017 2889
Size: 2017 872
SapI GCTCTTCn'nnn_
Cuts at: 0 248 319 769 1805 2889
Size: 248 71 450 1036 1084
Fragments arranged by size:
1084 1036 450 248 71
ScaI AGT'ACT
Cuts at: 0 226 2889
Size: 226 2663
SexAI A'CCwGG_T
Cuts at: 0 2730 2889
Size: 2730 159
SfcI C'TryA_G
Cuts at: 0 29 196 625 706 748 865 1099 2041
Size: 29 167 429 81 42 117 234 942
Cuts at: 2041 2722 2889
Size: 681 167
Fragments arranged by size:
942 681 429 234 167 167 117 81
42 29
SmaI CCC'GGG
Cuts at: 0 961 2889
Size: 961 1928
SmlI C'TyrA_G
Cuts at: 0 667 1651 1819 2476 2889
Size: 667 984 168 657 413
Fragments arranged by size:
984 667 657 413 168
SphI G_CATG'C
Cuts at: 0 1609 2889
Size: 1609 1280
StuI AGG'CCT
Cuts at: 0 172 563 2221 2506 2629 2889
Size: 172 391 1658 285 123 260
Fragments arranged by size:
1658 391 285 260 172 123
StyI C'CwwG_G
Cuts at: 0 53 915 1595 1611 2165 2862 2889
Size: 53 862 680 16 554 697 27
Fragments arranged by size:
862 697 680 554 53 27 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 334 657 675 2889
Size: 334 323 18 2214
Fragments arranged by size:
2214 334 323 18
TatI w'GTAC_w
Cuts at: 0 224 354 2201 2889
Size: 224 130 1847 688
Fragments arranged by size:
1847 688 224 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1835 1867 2098 2130 2720 2752 2889
Size: 1835 32 231 32 590 32 137
Fragments arranged by size:
1835 590 231 137 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1522 2889
Size: 1522 1367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 582 2028 2889
Size: 582 1446 861
Fragments arranged by size:
1446 861 582
XmaI C'CCGG_G
Cuts at: 0 959 2889
Size: 959 1930
XmnI GAAnn'nnTTC
Cuts at: 0 2836 2889
Size: 2836 53
ZraI GAC'GTC
Cuts at: 0 1443 2889
Size: 1443 1446
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AhdI AleI
AlwNI ApaI ApoI AvaI AvrII BaeI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HincII KpnI MfeI MmeI MscI MslI
MspA1I NcoI NruI NspI PasI PflMI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SanDI SapI ScaI
SexAI SfcI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflIII AgeI AlfI AloI ApaLI AscI
AseI AsiSI BamHI BbeI BfrBI BmgBI BmtI BsaWI
BsiWI BsmBI BspEI BsrDI BssHII BssSI BstBI BstEII
ClaI CspCI DraI DraIII EagI EciI EcoRI FseI
FspI FspAI HindIII HpaI KasI MluI NaeI NarI
NdeI NgoMIV NheI NotI NsiI PacI PciI PfoI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XbaI XhoI