Restriction map for
pF1KA0725
(Linear) (Six-base) MAPSORT of: KIAA0725.seq Check: 4684 from: 1 to: 2133
KIAA0725.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1449 2133
Size: 1449 684
AccI GT'mk_AC
Cuts at: 0 302 567 2133
Size: 302 265 1566
Fragments arranged by size:
1566 302 265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 1174 1255 1358 1817 1859 1874 2081
Size: 378 796 81 103 459 42 15 207
Cuts at: 2081 2133
Size: 52
Fragments arranged by size:
796 459 378 207 103 81 52 42
15
AflII C'TTAA_G
Cuts at: 0 1756 2133
Size: 1756 377
AlwNI CAG_nnn'CTG
Cuts at: 0 198 532 1864 2133
Size: 198 334 1332 269
Fragments arranged by size:
1332 334 269 198
ApoI r'AATT_y
Cuts at: 0 796 1201 1283 1666 2133
Size: 796 405 82 383 467
Fragments arranged by size:
796 467 405 383 82
BanII G_rGCy'C
Cuts at: 0 1812 2133
Size: 1812 321
BbsI GAAGACnn'nnnn_
Cuts at: 0 912 2133
Size: 912 1221
BciVI GTATCCnnnnn_n'
Cuts at: 0 1044 2133
Size: 1044 1089
BclI T'GATC_A
Cuts at: 0 2118 2133
Size: 2118 15
BglII A'GATC_T
Cuts at: 0 849 1266 2090 2133
Size: 849 417 824 43
Fragments arranged by size:
849 824 417 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 30 315 2061 2133
Size: 30 285 1746 72
Fragments arranged by size:
1746 285 72 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 618 2133
Size: 618 1515
BsaI GGTCTCn'nnnn_
Cuts at: 0 1347 1900 2133
Size: 1347 553 233
Fragments arranged by size:
1347 553 233
BsaAI yAC'GTr
Cuts at: 0 341 2133
Size: 341 1792
BsaBI GATnn'nnATC
Cuts at: 0 848 2133
Size: 848 1285
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 16 46 1794 1824 2133
Size: 16 30 1748 30 309
Fragments arranged by size:
1748 309 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 602 2133
Size: 602 1531
BseYI C'CCAG_C
Cuts at: 0 871 2133
Size: 871 1262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 2133
Size: 31 2102
BsiHKAI G_wGCw'C
Cuts at: 0 1812 2133
Size: 1812 321
BsmI GAATG_Cn'
Cuts at: 0 828 2133
Size: 828 1305
BsmBI CGTCTCn'nnnn_
Cuts at: 0 324 2133
Size: 324 1809
Bsp1286I G_dGCh'C
Cuts at: 0 1812 2133
Size: 1812 321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1449 2133
Size: 1449 684
BsrDI GCAATG_nn'
Cuts at: 0 1037 2133
Size: 1037 1096
BsrGI T'GTAC_A
Cuts at: 0 702 1255 2133
Size: 702 553 878
Fragments arranged by size:
878 702 553
BstBI TT'CG_AA
Cuts at: 0 693 2133
Size: 693 1440
BstXI CCAn_nnnn'nTGG
Cuts at: 0 667 812 2133
Size: 667 145 1321
Fragments arranged by size:
1321 667 145
BstYI r'GATC_y
Cuts at: 0 42 849 1266 1516 2090 2133
Size: 42 807 417 250 574 43
Fragments arranged by size:
807 574 417 250 43 42
BstZ17I GTA'TAC
Cuts at: 0 303 2133
Size: 303 1830
Bsu36I CC'TnA_GG
Cuts at: 0 886 2133
Size: 886 1247
BtgI C'CryG_G
Cuts at: 0 554 660 2133
Size: 554 106 1473
Fragments arranged by size:
1473 554 106
BtsI GCAGTG_nn'
Cuts at: 0 732 2133
Size: 732 1401
EarI CTCTTCn'nnn_
Cuts at: 0 197 577 1898 2133
Size: 197 380 1321 235
Fragments arranged by size:
1321 380 235 197
EcoICRI GAG'CTC
Cuts at: 0 1810 2133
Size: 1810 323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 1174 1255 1358 1817 1859 1874 2081
Size: 378 796 81 103 459 42 15 207
Cuts at: 2081 2133
Size: 52
Fragments arranged by size:
796 459 378 207 103 81 52 42
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1373 2133
Size: 1373 760
EcoRI G'AATT_C
Cuts at: 0 1201 1283 2133
Size: 1201 82 850
Fragments arranged by size:
1201 850 82
EcoRV GAT'ATC
Cuts at: 0 1077 1212 2133
Size: 1077 135 921
Fragments arranged by size:
1077 921 135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1782 2133
Size: 1782 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 1674 1706 2133
Size: 16 32 1626 32 427
Fragments arranged by size:
1626 427 32 32 16
HincII GTy'rAC
Cuts at: 0 568 609 810 1896 2133
Size: 568 41 201 1086 237
Fragments arranged by size:
1086 568 237 201 41
HindIII A'AGCT_T
Cuts at: 0 499 1829 2133
Size: 499 1330 304
Fragments arranged by size:
1330 499 304
HpaI GTT'AAC
Cuts at: 0 1896 2133
Size: 1896 237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1606 2133
Size: 1606 527
MslI CAynn'nnrTG
Cuts at: 0 1528 2133
Size: 1528 605
MspA1I CmG'CkG
Cuts at: 0 198 2133
Size: 198 1935
NcoI C'CATG_G
Cuts at: 0 554 660 2133
Size: 554 106 1473
Fragments arranged by size:
1473 554 106
NspI r_CATG'y
Cuts at: 0 423 2133
Size: 423 1710
PasI CC'CwG_GG
Cuts at: 0 2103 2133
Size: 2103 30
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 973 1005 2133
Size: 973 32 1128
Fragments arranged by size:
1128 973 32
PstI C_TGCA'G
Cuts at: 0 857 1411 2133
Size: 857 554 722
Fragments arranged by size:
857 722 554
PvuII CAG'CTG
Cuts at: 0 198 2133
Size: 198 1935
SacI G_AGCT'C
Cuts at: 0 1812 2133
Size: 1812 321
SalI G'TCGA_C
Cuts at: 0 566 2133
Size: 566 1567
SfcI C'TryA_G
Cuts at: 0 745 853 1003 1407 1572 1632 1987 2133
Size: 745 108 150 404 165 60 355 146
Fragments arranged by size:
745 404 355 165 150 146 108 60
SmlI C'TyrA_G
Cuts at: 0 1756 2133
Size: 1756 377
SspI AAT'ATT
Cuts at: 0 1137 1605 2133
Size: 1137 468 528
Fragments arranged by size:
1137 528 468
StyI C'CwwG_G
Cuts at: 0 554 660 2133
Size: 554 106 1473
Fragments arranged by size:
1473 554 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 567 1277 2133
Size: 567 710 856
Fragments arranged by size:
856 710 567
TatI w'GTAC_w
Cuts at: 0 702 1255 2133
Size: 702 553 878
Fragments arranged by size:
878 702 553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1746 2133
Size: 1746 387
XmnI GAAnn'nnTTC
Cuts at: 0 800 2133
Size: 800 1333
Enzymes that do cut:
AarI AccI AcuI AflII AlwNI ApoI BanII BbsI
BciVI BclI BglII BmrI BplI BsaI BsaAI BsaBI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrDI BsrGI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtsI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FalI Hin4I HincII HindIII HpaI MmeI MslI MspA1I
NcoI NspI PasI PsrI PstI PvuII SacI SalI
SfcI SmlI SspI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BpuEI
BsaHI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrFI
BssHII BssSI BstAPI BstEII BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoO109I FseI FspI
FspAI HaeII KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PvuI RsrII SacII SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI