Restriction map for
pF1KA0732
(Linear) (Six-base) MAPSORT of: KIAA0732.seq Check: 2489 from: 1 to: 4257
KIAA0732.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2968 4257
Size: 2968 1289
AccI GT'mk_AC
Cuts at: 0 2332 2521 3175 4257
Size: 2332 189 654 1082
Fragments arranged by size:
2332 1082 654 189
Acc65I G'GTAC_C
Cuts at: 0 2210 2261 4257
Size: 2210 51 1996
Fragments arranged by size:
2210 1996 51
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 2050 2287 3076 3412 3599 4257
Size: 895 1155 237 789 336 187 658
Fragments arranged by size:
1155 895 789 658 336 237 187
AfeI AGC'GCT
Cuts at: 0 727 801 1873 4257
Size: 727 74 1072 2384
Fragments arranged by size:
2384 1072 727 74
AflII C'TTAA_G
Cuts at: 0 2968 4257
Size: 2968 1289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3387 4257
Size: 3387 870
AlwNI CAG_nnn'CTG
Cuts at: 0 1546 1740 1776 1926 2073 2746 3577 4257
Size: 1546 194 36 150 147 673 831 680
Fragments arranged by size:
1546 831 680 673 194 150 147 36
ApaI G_GGCC'C
Cuts at: 0 1600 4248 4257
Size: 1600 2648 9
Fragments arranged by size:
2648 1600 9
ApoI r'AATT_y
Cuts at: 0 217 2479 4257
Size: 217 2262 1778
Fragments arranged by size:
2262 1778 217
AvaI C'yCGr_G
Cuts at: 0 1696 3291 3940 4257
Size: 1696 1595 649 317
Fragments arranged by size:
1696 1595 649 317
AvrII C'CTAG_G
Cuts at: 0 364 4257
Size: 364 3893
BamHI G'GATC_C
Cuts at: 0 50 1980 4257
Size: 50 1930 2277
Fragments arranged by size:
2277 1930 50
BanI G'GyrC_C
Cuts at: 0 1348 2210 2261 2297 3225 3468 3822 4257
Size: 1348 862 51 36 928 243 354 435
Fragments arranged by size:
1348 928 862 435 354 243 51 36
BanII G_rGCy'C
Cuts at: 0 610 718 1600 2030 2494 3722 3981 4248
Size: 610 108 882 430 464 1228 259 267
Cuts at: 4248 4257
Size: 9
Fragments arranged by size:
1228 882 610 464 430 267 259 108
9
BbeI G_GCGC'C
Cuts at: 0 2301 4257
Size: 2301 1956
BbsI GAAGACnn'nnnn_
Cuts at: 0 2108 2582 3029 3560 4257
Size: 2108 474 447 531 697
Fragments arranged by size:
2108 697 531 474 447
BbvCI CC'TCA_GC
Cuts at: 0 803 4257
Size: 803 3454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3643 3677 3912 3946 3984 4018 4257
Size: 3643 34 235 34 38 34 239
Fragments arranged by size:
3643 239 235 38 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1568 4257
Size: 1568 2689
BclI T'GATC_A
Cuts at: 0 1383 2165 3836 4257
Size: 1383 782 1671 421
Fragments arranged by size:
1671 1383 782 421
BfrBI ATG'CAT
Cuts at: 0 2183 4257
Size: 2183 2074
BglI GCCn_nnn'nGGC
Cuts at: 0 73 1346 3784 4140 4257
Size: 73 1273 2438 356 117
Fragments arranged by size:
2438 1273 356 117 73
BglII A'GATC_T
Cuts at: 0 141 431 2561 4257
Size: 141 290 2130 1696
Fragments arranged by size:
2130 1696 290 141
BlpI GC'TnA_GC
Cuts at: 0 780 1783 2987 3650 4257
Size: 780 1003 1204 663 607
Fragments arranged by size:
1204 1003 780 663 607
Bme1580I G_kGCm'C
Cuts at: 0 1600 2175 2602 3827 4248 4257
Size: 1600 575 427 1225 421 9
Fragments arranged by size:
1600 1225 575 427 421 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 1548 1585 2648 3065 4257
Size: 1548 37 1063 417 1192
Fragments arranged by size:
1548 1192 1063 417 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2907 3363 4257
Size: 2907 456 894
Fragments arranged by size:
2907 894 456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1256 1782 1846 2578 3709 3817 4257
Size: 34 1222 526 64 732 1131 108 440
Fragments arranged by size:
1222 1131 732 526 440 108 64 34
Bpu10I CC'TnA_GC
Cuts at: 0 175 803 1481 2488 4103 4257
Size: 175 628 678 1007 1615 154
Fragments arranged by size:
1615 1007 678 628 175 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1164 2743 4257
Size: 1164 1579 1514
Fragments arranged by size:
1579 1514 1164
BsaI GGTCTCn'nnnn_
Cuts at: 0 2428 4257
Size: 2428 1829
BsaAI yAC'GTr
Cuts at: 0 3336 4241 4257
Size: 3336 905 16
Fragments arranged by size:
3336 905 16
BsaHI Gr'CG_yC
Cuts at: 0 135 1411 2298 4257
Size: 135 1276 887 1959
Fragments arranged by size:
1959 1276 887 135
BsaWI w'CCGG_w
Cuts at: 0 1815 4175 4257
Size: 1815 2360 82
Fragments arranged by size:
2360 1815 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1780 1810 4257
Size: 1780 30 2447
Fragments arranged by size:
2447 1780 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 1714 2352 2896 3116 3547 3550 4144
Size: 505 1209 638 544 220 431 3 594
Cuts at: 4144 4257
Size: 113
Fragments arranged by size:
1209 638 594 544 505 431 220 113
3
BseYI C'CCAG_C
Cuts at: 0 1415 2174 2273 2637 2858 3886 4044 4227
Size: 1415 759 99 364 221 1028 158 183
Cuts at: 4227 4257
Size: 30
Fragments arranged by size:
1415 1028 759 364 221 183 158 99
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1726 2940 4257
Size: 1726 1214 1317
Fragments arranged by size:
1726 1317 1214
BsiHKAI G_wGCw'C
Cuts at: 0 2030 3722 4257
Size: 2030 1692 535
Fragments arranged by size:
2030 1692 535
BsmI GAATG_Cn'
Cuts at: 0 1220 1941 3439 4257
Size: 1220 721 1498 818
Fragments arranged by size:
1498 1220 818 721
Bsp1286I G_dGCh'C
Cuts at: 0 610 718 1600 2030 2175 2494 2602 3722
Size: 610 108 882 430 145 319 108 1120
Cuts at: 3722 3827 3981 4248 4257
Size: 105 154 267 9
Fragments arranged by size:
1120 882 610 430 319 267 154 145
108 108 105 9
BspEI T'CCGG_A
Cuts at: 0 1815 4257
Size: 1815 2442
BspHI T'CATG_A
Cuts at: 0 2417 4257
Size: 2417 1840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 434 1255 1401 1733 1799 2067 2968 4190
Size: 434 821 146 332 66 268 901 1222
Cuts at: 4190 4257
Size: 67
Fragments arranged by size:
1222 901 821 434 332 268 146 67
66
BsrBI CCG'CTC
Cuts at: 0 737 2891 4257
Size: 737 2154 1366
Fragments arranged by size:
2154 1366 737
BsrDI GCAATG_nn'
Cuts at: 0 2279 4002 4257
Size: 2279 1723 255
Fragments arranged by size:
2279 1723 255
BsrGI T'GTAC_A
Cuts at: 0 3233 4257
Size: 3233 1024
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2189 4257
Size: 2189 2068
BstXI CCAn_nnnn'nTGG
Cuts at: 0 486 2556 2698 3138 3627 3886 4257
Size: 486 2070 142 440 489 259 371
Fragments arranged by size:
2070 489 486 440 371 259 142
BstYI r'GATC_y
Cuts at: 0 26 50 141 431 1309 1324 1922 1980
Size: 26 24 91 290 878 15 598 58
Cuts at: 1980 2561 3141 3674 4257
Size: 581 580 533 583
Fragments arranged by size:
878 598 583 581 580 533 290 91
58 26 24 15
BstZ17I GTA'TAC
Cuts at: 0 2333 4257
Size: 2333 1924
Bsu36I CC'TnA_GG
Cuts at: 0 3002 3320 4257
Size: 3002 318 937
Fragments arranged by size:
3002 937 318
BtgI C'CryG_G
Cuts at: 0 702 999 1091 1341 2584 2697 3485 3990
Size: 702 297 92 250 1243 113 788 505
Cuts at: 3990 4257
Size: 267
Fragments arranged by size:
1243 788 702 505 297 267 250 113
92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2193 3996 4257
Size: 2193 1803 261
Fragments arranged by size:
2193 1803 261
BtsI GCAGTG_nn'
Cuts at: 0 1155 2333 2747 3215 4029 4257
Size: 1155 1178 414 468 814 228
Fragments arranged by size:
1178 1155 814 468 414 228
EaeI y'GGCC_r
Cuts at: 0 996 2927 3635 3776 3857 4038 4257
Size: 996 1931 708 141 81 181 219
Fragments arranged by size:
1931 996 708 219 181 141 81
EarI CTCTTCn'nnn_
Cuts at: 0 964 1052 2426 3269 3419 4257
Size: 964 88 1374 843 150 838
Fragments arranged by size:
1374 964 843 838 150 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 465 2520 2938 2967 4257
Size: 19 446 2055 418 29 1290
Fragments arranged by size:
2055 1290 446 418 29 19
EcoICRI GAG'CTC
Cuts at: 0 2028 3720 4257
Size: 2028 1692 537
Fragments arranged by size:
2028 1692 537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 895 1256 1782 1846 2050 2287 2578
Size: 34 861 361 526 64 204 237 291
Cuts at: 2578 3076 3412 3599 3709 3817 4257
Size: 498 336 187 110 108 440
Fragments arranged by size:
861 526 498 440 361 336 291 237
204 187 110 108 64 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 363 508 1136 3274 3859 4257
Size: 363 145 628 2138 585 398
Fragments arranged by size:
2138 628 585 398 363 145
EcoO109I rG'GnC_Cy
Cuts at: 0 946 1291 1368 1543 1597 1621 1821 2754
Size: 946 345 77 175 54 24 200 933
Cuts at: 2754 3240 3324 4257
Size: 486 84 933
Fragments arranged by size:
946 933 933 486 345 200 175 84
77 54 24
EcoRI G'AATT_C
Cuts at: 0 217 4257
Size: 217 4040
FspI TGC'GCA
Cuts at: 0 2371 4257
Size: 2371 1886
HaeII r_GCGC'y
Cuts at: 0 729 803 1875 2301 4198 4257
Size: 729 74 1072 426 1897 59
Fragments arranged by size:
1897 1072 729 426 74 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2860 2892 3133 3157 3165 3189 4257
Size: 2860 32 241 24 8 24 1068
Fragments arranged by size:
2860 1068 241 32 24 24 8
HincII GTy'rAC
Cuts at: 0 3176 4166 4257
Size: 3176 990 91
Fragments arranged by size:
3176 990 91
HindIII A'AGCT_T
Cuts at: 0 2344 4257
Size: 2344 1913
KasI G'GCGC_C
Cuts at: 0 2297 4257
Size: 2297 1960
KpnI G_GTAC'C
Cuts at: 0 2214 2265 4257
Size: 2214 51 1992
Fragments arranged by size:
2214 1992 51
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 2517 4257
Size: 17 2500 1740
Fragments arranged by size:
2500 1740 17
MscI TGG'CCA
Cuts at: 0 2929 3637 3778 3859 4040 4257
Size: 2929 708 141 81 181 217
Fragments arranged by size:
2929 708 217 181 141 81
MslI CAynn'nnrTG
Cuts at: 0 2696 2836 3031 3817 4257
Size: 2696 140 195 786 440
Fragments arranged by size:
2696 786 440 195 140
MspA1I CmG'CkG
Cuts at: 0 704 784 2862 2954 2991 3367 3587 4044
Size: 704 80 2078 92 37 376 220 457
Cuts at: 4044 4257
Size: 213
Fragments arranged by size:
2078 704 457 376 220 213 92 80
37
NarI GG'CG_CC
Cuts at: 0 2298 4257
Size: 2298 1959
NcoI C'CATG_G
Cuts at: 0 1091 2697 3485 4257
Size: 1091 1606 788 772
Fragments arranged by size:
1606 1091 788 772
NsiI A_TGCA'T
Cuts at: 0 2185 4257
Size: 2185 2072
NspI r_CATG'y
Cuts at: 0 2536 2817 3157 4257
Size: 2536 281 340 1100
Fragments arranged by size:
2536 1100 340 281
PasI CC'CwG_GG
Cuts at: 0 205 1618 2595 4257
Size: 205 1413 977 1662
Fragments arranged by size:
1662 1413 977 205
PflMI CCAn_nnn'nTGG
Cuts at: 0 616 4257
Size: 616 3641
PfoI T'CCnGG_A
Cuts at: 0 2900 3047 3269 3686 4257
Size: 2900 147 222 417 571
Fragments arranged by size:
2900 571 417 222 147
PmlI CAC'GTG
Cuts at: 0 4241 4257
Size: 4241 16
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1766 1798 4257
Size: 1766 32 2459
Fragments arranged by size:
2459 1766 32
PpuMI rG'GwC_Cy
Cuts at: 0 946 1291 1368 2754 3240 3324 4257
Size: 946 345 77 1386 486 84 933
Fragments arranged by size:
1386 946 933 486 345 84 77
PshAI GACnn'nnGTC
Cuts at: 0 3698 4257
Size: 3698 559
PspOMI G'GGCC_C
Cuts at: 0 1596 4244 4257
Size: 1596 2648 13
Fragments arranged by size:
2648 1596 13
PspXI vC'TCGA_Gb
Cuts at: 0 3940 4257
Size: 3940 317
PstI C_TGCA'G
Cuts at: 0 431 1730 1781 1862 2078 2742 2783 2822
Size: 431 1299 51 81 216 664 41 39
Cuts at: 2822 3210 4257
Size: 388 1047
Fragments arranged by size:
1299 1047 664 431 388 216 81 51
41 39
PvuII CAG'CTG
Cuts at: 0 784 2862 2991 3367 4044 4257
Size: 784 2078 129 376 677 213
Fragments arranged by size:
2078 784 677 376 213 129
SacI G_AGCT'C
Cuts at: 0 2030 3722 4257
Size: 2030 1692 535
Fragments arranged by size:
2030 1692 535
SacII CC_GC'GG
Cuts at: 0 705 4257
Size: 705 3552
SalI G'TCGA_C
Cuts at: 0 3174 4257
Size: 3174 1083
SanDI GG'GwC_CC
Cuts at: 0 1368 4257
Size: 1368 2889
SapI GCTCTTCn'nnn_
Cuts at: 0 3419 4257
Size: 3419 838
SexAI A'CCwGG_T
Cuts at: 0 509 4257
Size: 509 3748
SfcI C'TryA_G
Cuts at: 0 326 427 1582 1726 1777 1858 2074 2738
Size: 326 101 1155 144 51 81 216 664
Cuts at: 2738 2779 2818 3206 4257
Size: 41 39 388 1051
Fragments arranged by size:
1155 1051 664 388 326 216 144 101
81 51 41 39
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 73 4257
Size: 73 4184
SfoI GGC'GCC
Cuts at: 0 2299 4257
Size: 2299 1958
SmlI C'TyrA_G
Cuts at: 0 1143 2758 2968 3940 4257
Size: 1143 1615 210 972 317
Fragments arranged by size:
1615 1143 972 317 210
SphI G_CATG'C
Cuts at: 0 2817 4257
Size: 2817 1440
StyI C'CwwG_G
Cuts at: 0 364 857 1091 2193 2405 2697 3485 3860
Size: 364 493 234 1102 212 292 788 375
Cuts at: 3860 4257
Size: 397
Fragments arranged by size:
1102 788 493 397 375 364 292 234
212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 257 4257
Size: 257 4000
TatI w'GTAC_w
Cuts at: 0 2847 3233 3809 4257
Size: 2847 386 576 448
Fragments arranged by size:
2847 576 448 386
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 526 558 1646 1677 1678 1709 3105 3137
Size: 526 32 1088 31 1 31 1396 32
Cuts at: 3137 3443 3475 4170 4202 4257
Size: 306 32 695 32 55
Fragments arranged by size:
1396 1088 695 526 306 55 32 32
32 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 0 1647 2353 4257
Size: 1647 706 1904
Fragments arranged by size:
1904 1647 706
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 316 1113 4257
Size: 316 797 3144
Fragments arranged by size:
3144 797 316
XhoI C'TCGA_G
Cuts at: 0 3940 4257
Size: 3940 317
XmnI GAAnn'nnTTC
Cuts at: 0 359 1939 2006 4257
Size: 359 1580 67 2251
Fragments arranged by size:
2251 1580 359 67
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AlfI AlwNI
ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI
BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NarI NcoI NsiI NspI PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SacII SalI SanDI SapI SexAI
SfcI SfiI SfoI SmlI SphI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AflIII AgeI AhdI AleI AloI ApaLI
AscI AseI AsiSI BaeI BmgBI BmtI BsaBI BsiEI
BsiWI BsmBI BsrFI BssHII BssSI BstBI BstEII ClaI
CspCI DraI DraIII DrdI EagI EcoRV FalI FseI
FspAI HpaI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI PacI PciI PmeI PsiI PsrI PvuI
RsrII SbfI ScaI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI XbaI XmaI ZraI