Restriction map for
pF1KSDA0733
(Linear) (Six-base) MAPSORT of: KIAA0733.seq Check: 6869 from: 1 to: 2079
KIAA0733.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 210 848 2079
Size: 210 638 1231
Fragments arranged by size:
1231 638 210
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 92 1043 2079
Size: 83 9 951 1036
Fragments arranged by size:
1036 951 83 9
AfeI AGC'GCT
Cuts at: 0 1878 2079
Size: 1878 201
AflIII A'CryG_T
Cuts at: 0 1262 1536 2079
Size: 1262 274 543
Fragments arranged by size:
1262 543 274
AhdI GACnn_n'nnGTC
Cuts at: 0 773 2079
Size: 773 1306
AlwNI CAG_nnn'CTG
Cuts at: 0 1782 2079
Size: 1782 297
ApoI r'AATT_y
Cuts at: 0 55 180 200 447 630 1740 2079
Size: 55 125 20 247 183 1110 339
Fragments arranged by size:
1110 339 247 183 125 55 20
AvaI C'yCGr_G
Cuts at: 0 1352 1853 2079
Size: 1352 501 226
Fragments arranged by size:
1352 501 226
BanI G'GyrC_C
Cuts at: 0 1724 2079
Size: 1724 355
BanII G_rGCy'C
Cuts at: 0 1998 2079
Size: 1998 81
BbeI G_GCGC'C
Cuts at: 0 1728 2079
Size: 1728 351
BbsI GAAGACnn'nnnn_
Cuts at: 0 908 2079
Size: 908 1171
BbvCI CC'TCA_GC
Cuts at: 0 731 2079
Size: 731 1348
BciVI GTATCCnnnnn_n'
Cuts at: 0 93 2079
Size: 93 1986
BclI T'GATC_A
Cuts at: 0 1482 2079
Size: 1482 597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1029 1239 1259 1789 2079
Size: 1029 210 20 530 290
Fragments arranged by size:
1029 530 290 210 20
Bpu10I CC'TnA_GC
Cuts at: 0 731 2079
Size: 731 1348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 386 1672 1955 2079
Size: 377 9 1286 283 124
Fragments arranged by size:
1286 377 283 124 9
BsaBI GATnn'nnATC
Cuts at: 0 979 2079
Size: 979 1100
BsaHI Gr'CG_yC
Cuts at: 0 1725 2079
Size: 1725 354
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1389 1419 2079
Size: 1389 30 660
Fragments arranged by size:
1389 660 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1340 2079
Size: 1340 739
BseYI C'CCAG_C
Cuts at: 0 701 1873 2079
Size: 701 1172 206
Fragments arranged by size:
1172 701 206
BsiHKAI G_wGCw'C
Cuts at: 0 1998 2079
Size: 1998 81
BsmI GAATG_Cn'
Cuts at: 0 693 1179 2079
Size: 693 486 900
Fragments arranged by size:
900 693 486
Bsp1286I G_dGCh'C
Cuts at: 0 1998 2079
Size: 1998 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 2079
Size: 53 2026
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1249 2079
Size: 1249 830
BstYI r'GATC_y
Cuts at: 0 1576 2079
Size: 1576 503
Bsu36I CC'TnA_GG
Cuts at: 0 1154 2079
Size: 1154 925
BtgI C'CryG_G
Cuts at: 0 267 2079
Size: 267 1812
EaeI y'GGCC_r
Cuts at: 0 342 2079
Size: 342 1737
EarI CTCTTCn'nnn_
Cuts at: 0 267 1062 1225 2079
Size: 267 795 163 854
Fragments arranged by size:
854 795 267 163
EcoICRI GAG'CTC
Cuts at: 0 1996 2079
Size: 1996 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 92 1029 1043 1239 1259 1789 2079
Size: 83 9 937 14 196 20 530 290
Fragments arranged by size:
937 530 290 196 83 20 14 9
EcoO109I rG'GnC_Cy
Cuts at: 0 958 1285 1915 2079
Size: 958 327 630 164
Fragments arranged by size:
958 630 327 164
EcoRV GAT'ATC
Cuts at: 0 157 2079
Size: 157 1922
HaeII r_GCGC'y
Cuts at: 0 1728 1880 2079
Size: 1728 152 199
Fragments arranged by size:
1728 199 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 759 791 1123 1155 1269 1301
Size: 232 32 495 32 332 32 114 32
Cuts at: 1301 1346 1378 1930 1962 2079
Size: 45 32 552 32 117
Fragments arranged by size:
552 495 332 232 117 114 45 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1150 2079
Size: 1150 929
KasI G'GCGC_C
Cuts at: 0 1724 2079
Size: 1724 355
MscI TGG'CCA
Cuts at: 0 344 2079
Size: 344 1735
MslI CAynn'nnrTG
Cuts at: 0 590 2079
Size: 590 1489
MspA1I CmG'CkG
Cuts at: 0 1782 2079
Size: 1782 297
NarI GG'CG_CC
Cuts at: 0 1725 2079
Size: 1725 354
NcoI C'CATG_G
Cuts at: 0 267 2079
Size: 267 1812
NspI r_CATG'y
Cuts at: 0 96 855 1266 1540 2079
Size: 96 759 411 274 539
Fragments arranged by size:
759 539 411 274 96
PciI A'CATG_T
Cuts at: 0 1262 1536 2079
Size: 1262 274 543
Fragments arranged by size:
1262 543 274
PflMI CCAn_nnn'nTGG
Cuts at: 0 463 2079
Size: 463 1616
PfoI T'CCnGG_A
Cuts at: 0 1255 2079
Size: 1255 824
PpuMI rG'GwC_Cy
Cuts at: 0 958 1285 1915 2079
Size: 958 327 630 164
Fragments arranged by size:
958 630 327 164
PspXI vC'TCGA_Gb
Cuts at: 0 1352 2079
Size: 1352 727
PstI C_TGCA'G
Cuts at: 0 1425 2079
Size: 1425 654
PvuII CAG'CTG
Cuts at: 0 1782 2079
Size: 1782 297
SacI G_AGCT'C
Cuts at: 0 1998 2079
Size: 1998 81
SfcI C'TryA_G
Cuts at: 0 1421 2079
Size: 1421 658
SfoI GGC'GCC
Cuts at: 0 1726 2079
Size: 1726 353
SmlI C'TyrA_G
Cuts at: 0 392 401 1352 1651 1970 2079
Size: 392 9 951 299 319 109
Fragments arranged by size:
951 392 319 299 109 9
SspI AAT'ATT
Cuts at: 0 948 1506 1902 2079
Size: 948 558 396 177
Fragments arranged by size:
948 558 396 177
StuI AGG'CCT
Cuts at: 0 648 2079
Size: 648 1431
StyI C'CwwG_G
Cuts at: 0 6 267 2068 2079
Size: 6 261 1801 11
Fragments arranged by size:
1801 261 11 6
TatI w'GTAC_w
Cuts at: 0 148 609 2017 2079
Size: 148 461 1408 62
Fragments arranged by size:
1408 461 148 62
XhoI C'TCGA_G
Cuts at: 0 1352 2079
Size: 1352 727
XmnI GAAnn'nnTTC
Cuts at: 0 362 1008 1637 2079
Size: 362 646 629 442
Fragments arranged by size:
646 629 442 362
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AlwNI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BciVI BclI BpmI
Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspMI BstAPI BstYI Bsu36I BtgI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII
KasI MscI MslI MspA1I NarI NcoI NspI PciI
PflMI PfoI PpuMI PspXI PstI PvuII SacI SfcI
SfoI SmlI SspI StuI StyI TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BsaI BsaAI BsaWI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI
EcoRI FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI TaqII TstI Tth111I
XbaI XcmI XmaI ZraI