Restriction map for
pF1KSDA0735
(Linear) (Six-base) MAPSORT of: KIAA0735.seq Check: 1191 from: 1 to: 2049
KIAA0735.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 461 2049
Size: 461 1588
Acc65I G'GTAC_C
Cuts at: 0 1076 2049
Size: 1076 973
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 777 901 2049
Size: 777 124 1148
Fragments arranged by size:
1148 777 124
AfeI AGC'GCT
Cuts at: 0 1084 2049
Size: 1084 965
AflIII A'CryG_T
Cuts at: 0 416 1395 2049
Size: 416 979 654
Fragments arranged by size:
979 654 416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1752 2049
Size: 1752 297
AlwNI CAG_nnn'CTG
Cuts at: 0 1791 2043 2049
Size: 1791 252 6
Fragments arranged by size:
1791 252 6
ApaI G_GGCC'C
Cuts at: 0 1154 2049
Size: 1154 895
ApoI r'AATT_y
Cuts at: 0 673 1042 1903 2049
Size: 673 369 861 146
Fragments arranged by size:
861 673 369 146
AvaI C'yCGr_G
Cuts at: 0 683 1651 2030 2049
Size: 683 968 379 19
Fragments arranged by size:
968 683 379 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 138 171 2049
Size: 138 33 1878
Fragments arranged by size:
1878 138 33
BamHI G'GATC_C
Cuts at: 0 326 2049
Size: 326 1723
BanI G'GyrC_C
Cuts at: 0 198 481 1076 1309 1909 2049
Size: 198 283 595 233 600 140
Fragments arranged by size:
600 595 283 233 198 140
BanII G_rGCy'C
Cuts at: 0 901 1154 2049
Size: 901 253 895
Fragments arranged by size:
901 895 253
BbeI G_GCGC'C
Cuts at: 0 202 485 1313 1913 2049
Size: 202 283 828 600 136
Fragments arranged by size:
828 600 283 202 136
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 1445 2049
Size: 131 1314 604
Fragments arranged by size:
1314 604 131
BbvCI CC'TCA_GC
Cuts at: 0 532 2049
Size: 532 1517
BciVI GTATCCnnnnn_n'
Cuts at: 0 166 1862 2049
Size: 166 1696 187
Fragments arranged by size:
1696 187 166
BclI T'GATC_A
Cuts at: 0 1835 2049
Size: 1835 214
BglI GCCn_nnn'nGGC
Cuts at: 0 187 196 224 358 2049
Size: 187 9 28 134 1691
Fragments arranged by size:
1691 187 134 28 9
BglII A'GATC_T
Cuts at: 0 131 2049
Size: 131 1918
Bme1580I G_kGCm'C
Cuts at: 0 1154 2049
Size: 1154 895
BmrI ACTGGGnnnn_n'
Cuts at: 0 38 395 743 2049
Size: 38 357 348 1306
Fragments arranged by size:
1306 357 348 38
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 619 721 2049
Size: 619 102 1328
Fragments arranged by size:
1328 619 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 783 846 1579 2049
Size: 189 594 63 733 470
Fragments arranged by size:
733 594 470 189 63
Bpu10I CC'TnA_GC
Cuts at: 0 532 980 2049
Size: 532 448 1069
Fragments arranged by size:
1069 532 448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 1681 2049
Size: 934 747 368
Fragments arranged by size:
934 747 368
BsaI GGTCTCn'nnnn_
Cuts at: 0 280 2049
Size: 280 1769
BsaHI Gr'CG_yC
Cuts at: 0 199 482 1310 1910 2049
Size: 199 283 828 600 139
Fragments arranged by size:
828 600 283 199 139
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 710 2049
Size: 277 433 1339
Fragments arranged by size:
1339 433 277
BseYI C'CCAG_C
Cuts at: 0 513 714 785 2049
Size: 513 201 71 1264
Fragments arranged by size:
1264 513 201 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 604 843 2049
Size: 425 179 239 1206
Fragments arranged by size:
1206 425 239 179
BsiHKAI G_wGCw'C
Cuts at: 0 645 852 1520 2049
Size: 645 207 668 529
Fragments arranged by size:
668 645 529 207
BsmI GAATG_Cn'
Cuts at: 0 449 1201 1827 1883 2049
Size: 449 752 626 56 166
Fragments arranged by size:
752 626 449 166 56
Bsp1286I G_dGCh'C
Cuts at: 0 645 852 901 1154 1520 2049
Size: 645 207 49 253 366 529
Fragments arranged by size:
645 529 366 253 207 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1107 2049
Size: 1107 942
BsrDI GCAATG_nn'
Cuts at: 0 72 1778 1808 2049
Size: 72 1706 30 241
Fragments arranged by size:
1706 241 72 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1035 1351 1557 1974 2049
Size: 1035 316 206 417 75
Fragments arranged by size:
1035 417 316 206 75
BstYI r'GATC_y
Cuts at: 0 131 326 1052 1340 2049
Size: 131 195 726 288 709
Fragments arranged by size:
726 709 288 195 131
BtgI C'CryG_G
Cuts at: 0 60 309 755 869 1181 1196 2049
Size: 60 249 446 114 312 15 853
Fragments arranged by size:
853 446 312 249 114 60 15
BtsI GCAGTG_nn'
Cuts at: 0 1792 2049
Size: 1792 257
DraI TTT'AAA
Cuts at: 0 1392 2049
Size: 1392 657
DraIII CAC_nnn'GTG
Cuts at: 0 97 2049
Size: 97 1952
EaeI y'GGCC_r
Cuts at: 0 306 312 359 545 1178 2049
Size: 306 6 47 186 633 871
Fragments arranged by size:
871 633 306 186 47 6
EarI CTCTTCn'nnn_
Cuts at: 0 579 610 2049
Size: 579 31 1439
Fragments arranged by size:
1439 579 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 777 783 846 901 1579 2049
Size: 189 588 6 63 55 678 470
Fragments arranged by size:
678 588 470 189 63 55 6
EcoO109I rG'GnC_Cy
Cuts at: 0 738 986 1150 1151 2040 2049
Size: 738 248 164 1 889 9
Fragments arranged by size:
889 738 248 164 9 1
EcoRI G'AATT_C
Cuts at: 0 673 1042 2049
Size: 673 369 1007
Fragments arranged by size:
1007 673 369
HaeII r_GCGC'y
Cuts at: 0 202 485 1086 1313 1913 2049
Size: 202 283 601 227 600 136
Fragments arranged by size:
601 600 283 227 202 136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 821 853 1320 1352 1432 1464 2024 2049
Size: 821 32 467 32 80 32 560 25
Fragments arranged by size:
821 560 467 80 32 32 32 25
KasI G'GCGC_C
Cuts at: 0 198 481 1309 1909 2049
Size: 198 283 828 600 140
Fragments arranged by size:
828 600 283 198 140
KpnI G_GTAC'C
Cuts at: 0 1080 2049
Size: 1080 969
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 1037 2049
Size: 98 939 1012
Fragments arranged by size:
1012 939 98
MscI TGG'CCA
Cuts at: 0 308 2049
Size: 308 1741
MslI CAynn'nnrTG
Cuts at: 0 167 970 1349 1381 2049
Size: 167 803 379 32 668
Fragments arranged by size:
803 668 379 167 32
MspA1I CmG'CkG
Cuts at: 0 62 2049
Size: 62 1987
NarI GG'CG_CC
Cuts at: 0 199 482 1310 1910 2049
Size: 199 283 828 600 139
Fragments arranged by size:
828 600 283 199 139
NcoI C'CATG_G
Cuts at: 0 309 755 869 1196 2049
Size: 309 446 114 327 853
Fragments arranged by size:
853 446 327 309 114
NspI r_CATG'y
Cuts at: 0 420 540 1303 1399 2049
Size: 420 120 763 96 650
Fragments arranged by size:
763 650 420 120 96
PciI A'CATG_T
Cuts at: 0 416 1395 2049
Size: 416 979 654
Fragments arranged by size:
979 654 416
PflMI CCAn_nnn'nTGG
Cuts at: 0 761 781 2049
Size: 761 20 1268
Fragments arranged by size:
1268 761 20
PfoI T'CCnGG_A
Cuts at: 0 1556 2049
Size: 1556 493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 985 1017 1507 1539 2049
Size: 985 32 490 32 510
Fragments arranged by size:
985 510 490 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 986 2040 2049
Size: 986 1054 9
Fragments arranged by size:
1054 986 9
PshAI GACnn'nnGTC
Cuts at: 0 419 2049
Size: 419 1630
PspOMI G'GGCC_C
Cuts at: 0 1150 2049
Size: 1150 899
PstI C_TGCA'G
Cuts at: 0 1731 2049
Size: 1731 318
SacII CC_GC'GG
Cuts at: 0 63 2049
Size: 63 1986
SanDI GG'GwC_CC
Cuts at: 0 986 2049
Size: 986 1063
SexAI A'CCwGG_T
Cuts at: 0 1072 2049
Size: 1072 977
SfcI C'TryA_G
Cuts at: 0 1155 1727 2049
Size: 1155 572 322
Fragments arranged by size:
1155 572 322
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 187 358 2049
Size: 187 171 1691
Fragments arranged by size:
1691 187 171
SfoI GGC'GCC
Cuts at: 0 200 483 1311 1911 2049
Size: 200 283 828 600 138
Fragments arranged by size:
828 600 283 200 138
SmaI CCC'GGG
Cuts at: 0 1653 2049
Size: 1653 396
SmlI C'TyrA_G
Cuts at: 0 949 1696 2030 2049
Size: 949 747 334 19
Fragments arranged by size:
949 747 334 19
StuI AGG'CCT
Cuts at: 0 500 2049
Size: 500 1549
StyI C'CwwG_G
Cuts at: 0 309 755 869 901 1196 2049
Size: 309 446 114 32 295 853
Fragments arranged by size:
853 446 309 295 114 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 179 2049
Size: 179 1870
TatI w'GTAC_w
Cuts at: 0 1136 1385 1398 2049
Size: 1136 249 13 651
Fragments arranged by size:
1136 651 249 13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 782 2049
Size: 782 1267
XhoI C'TCGA_G
Cuts at: 0 2030 2049
Size: 2030 19
XmaI C'CCGG_G
Cuts at: 0 1651 2049
Size: 1651 398
XmnI GAAnn'nnTTC
Cuts at: 0 1000 2049
Size: 1000 1049
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AlfI AlwNI ApaI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BstXI BstYI BtgI
BtsI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRI
HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NspI PciI PflMI PfoI PpiI PpuMI
PshAI PspOMI PstI SacII SanDI SexAI SfcI SfiI
SfoI SmaI SmlI StuI StyI TaqII TatI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AleI AloI
ApaLI AscI AseI AsiSI AvrII BcgI BfrBI BlpI
BmgBI BmtI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DrdI EagI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
ZraI