Restriction map for
pF1KSDA0738
(Linear) (Six-base) MAPSORT of: KIAA0738.seq Check: 3476 from: 1 to: 2763
KIAA0738.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2341 2763
Size: 2341 422
AccI GT'mk_AC
Cuts at: 0 998 2763
Size: 998 1765
Acc65I G'GTAC_C
Cuts at: 0 2183 2763
Size: 2183 580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1706 1729 2763
Size: 1706 23 1034
Fragments arranged by size:
1706 1034 23
AflIII A'CryG_T
Cuts at: 0 1373 2763
Size: 1373 1390
AgeI A'CCGG_T
Cuts at: 0 1784 2763
Size: 1784 979
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2290 2322 2763
Size: 2290 32 441
Fragments arranged by size:
2290 441 32
ApaI G_GGCC'C
Cuts at: 0 850 1202 1979 2263 2763
Size: 850 352 777 284 500
Fragments arranged by size:
850 777 500 352 284
AvaI C'yCGr_G
Cuts at: 0 313 1433 2763
Size: 313 1120 1330
Fragments arranged by size:
1330 1120 313
BanI G'GyrC_C
Cuts at: 0 294 1615 2183 2763
Size: 294 1321 568 580
Fragments arranged by size:
1321 580 568 294
BanII G_rGCy'C
Cuts at: 0 270 322 850 974 1083 1202 1646 1979
Size: 270 52 528 124 109 119 444 333
Cuts at: 1979 2114 2222 2263 2763
Size: 135 108 41 500
Fragments arranged by size:
528 500 444 333 270 135 124 119
109 108 52 41
BciVI GTATCCnnnnn_n'
Cuts at: 0 1969 2763
Size: 1969 794
BfrBI ATG'CAT
Cuts at: 0 2175 2476 2763
Size: 2175 301 287
Fragments arranged by size:
2175 301 287
BglI GCCn_nnn'nGGC
Cuts at: 0 25 893 2763
Size: 25 868 1870
Fragments arranged by size:
1870 868 25
BglII A'GATC_T
Cuts at: 0 2156 2763
Size: 2156 607
BlpI GC'TnA_GC
Cuts at: 0 2108 2763
Size: 2108 655
Bme1580I G_kGCm'C
Cuts at: 0 850 1202 1979 2263 2763
Size: 850 352 777 284 500
Fragments arranged by size:
850 777 500 352 284
BmgBI CAC'GTC
Cuts at: 0 2151 2763
Size: 2151 612
BmrI ACTGGGnnnn_n'
Cuts at: 0 601 853 1649 1879 2110 2407 2763
Size: 601 252 796 230 231 297 356
Fragments arranged by size:
796 601 356 297 252 231 230
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1789 2763
Size: 1789 974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1960 2062 2224 2491 2763
Size: 344 1616 102 162 267 272
Fragments arranged by size:
1616 344 272 267 162 102
Bpu10I CC'TnA_GC
Cuts at: 0 2051 2443 2763
Size: 2051 392 320
Fragments arranged by size:
2051 392 320
BsaI GGTCTCn'nnnn_
Cuts at: 0 1924 2763
Size: 1924 839
BsaAI yAC'GTr
Cuts at: 0 1820 2763
Size: 1820 943
BsaWI w'CCGG_w
Cuts at: 0 1784 2763
Size: 1784 979
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1228 1258 2290 2320 2763
Size: 1228 30 1032 30 443
Fragments arranged by size:
1228 1032 443 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 144 928 1138 1141 1828 1957 2734 2763
Size: 144 784 210 3 687 129 777 29
Fragments arranged by size:
784 777 687 210 144 129 29 3
BseYI C'CCAG_C
Cuts at: 0 50 212 707 1317 1923 2274 2763
Size: 50 162 495 610 606 351 489
Fragments arranged by size:
610 606 495 489 351 162 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1352 1587 2173 2233 2650 2763
Size: 928 424 235 586 60 417 113
Fragments arranged by size:
928 586 424 417 235 113 60
BsiEI CG_ry'CG
Cuts at: 0 2024 2763
Size: 2024 739
BsiHKAI G_wGCw'C
Cuts at: 0 2222 2763
Size: 2222 541
BsmI GAATG_Cn'
Cuts at: 0 2375 2478 2763
Size: 2375 103 285
Fragments arranged by size:
2375 285 103
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2356 2545 2763
Size: 2356 189 218
Fragments arranged by size:
2356 218 189
Bsp1286I G_dGCh'C
Cuts at: 0 270 322 850 974 1083 1202 1646 1979
Size: 270 52 528 124 109 119 444 333
Cuts at: 1979 2114 2222 2263 2763
Size: 135 108 41 500
Fragments arranged by size:
528 500 444 333 270 135 124 119
109 108 52 41
BspHI T'CATG_A
Cuts at: 0 422 2763
Size: 422 2341
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2341 2763
Size: 2341 422
BsrBI CCG'CTC
Cuts at: 0 1244 2763
Size: 1244 1519
BsrDI GCAATG_nn'
Cuts at: 0 143 2763
Size: 143 2620
BsrFI r'CCGG_y
Cuts at: 0 1784 2763
Size: 1784 979
BsrGI T'GTAC_A
Cuts at: 0 279 1547 2763
Size: 279 1268 1216
Fragments arranged by size:
1268 1216 279
BssSI C'ACGA_G
Cuts at: 0 520 567 2763
Size: 520 47 2196
Fragments arranged by size:
2196 520 47
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1494 1979 2763
Size: 1494 485 784
Fragments arranged by size:
1494 784 485
BstYI r'GATC_y
Cuts at: 0 602 1361 2156 2763
Size: 602 759 795 607
Fragments arranged by size:
795 759 607 602
Bsu36I CC'TnA_GG
Cuts at: 0 1393 2763
Size: 1393 1370
BtgI C'CryG_G
Cuts at: 0 165 759 1994 2763
Size: 165 594 1235 769
Fragments arranged by size:
1235 769 594 165
BtsI GCAGTG_nn'
Cuts at: 0 245 950 1678 2763
Size: 245 705 728 1085
Fragments arranged by size:
1085 728 705 245
ClaI AT'CG_AT
Cuts at: 0 1813 2763
Size: 1813 950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1072 1107 2763
Size: 1072 35 1656
Fragments arranged by size:
1656 1072 35
DraI TTT'AAA
Cuts at: 0 579 2763
Size: 579 2184
DraIII CAC_nnn'GTG
Cuts at: 0 2628 2763
Size: 2628 135
EaeI y'GGCC_r
Cuts at: 0 162 168 302 789 813 1253 1484 1991
Size: 162 6 134 487 24 440 231 507
Cuts at: 1991 2763
Size: 772
Fragments arranged by size:
772 507 487 440 231 162 134 24
6
EarI CTCTTCn'nnn_
Cuts at: 0 261 644 1276 1352 1945 2419 2548 2763
Size: 261 383 632 76 593 474 129 215
Fragments arranged by size:
632 593 474 383 261 215 129 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2053 2763
Size: 2053 710
EcoICRI GAG'CTC
Cuts at: 0 2220 2763
Size: 2220 543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1706 1729 1960 2062 2224 2491 2763
Size: 344 1362 23 231 102 162 267 272
Fragments arranged by size:
1362 344 272 267 231 162 102 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1217 2446 2763
Size: 1217 1229 317
Fragments arranged by size:
1229 1217 317
EcoO109I rG'GnC_Cy
Cuts at: 0 27 138 847 1198 1199 1229 1975 1976
Size: 27 111 709 351 1 30 746 1
Cuts at: 1976 2259 2260 2452 2763
Size: 283 1 192 311
Fragments arranged by size:
746 709 351 311 283 192 111 30
27 1 1 1
EcoRV GAT'ATC
Cuts at: 0 1414 2763
Size: 1414 1349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 2013 2045 2420 2436 2452 2468
Size: 619 32 1362 32 375 16 16 16
Cuts at: 2468 2763
Size: 295
Fragments arranged by size:
1362 619 375 295 32 32 16 16
16
HincII GTy'rAC
Cuts at: 0 1599 2589 2763
Size: 1599 990 174
Fragments arranged by size:
1599 990 174
HindIII A'AGCT_T
Cuts at: 0 1756 2763
Size: 1756 1007
KpnI G_GTAC'C
Cuts at: 0 2187 2763
Size: 2187 576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 1056 2763
Size: 662 394 1707
Fragments arranged by size:
1707 662 394
MscI TGG'CCA
Cuts at: 0 304 815 1486 1993 2763
Size: 304 511 671 507 770
Fragments arranged by size:
770 671 511 507 304
MslI CAynn'nnrTG
Cuts at: 0 764 1378 1733 2752 2763
Size: 764 614 355 1019 11
Fragments arranged by size:
1019 764 614 355 11
MspA1I CmG'CkG
Cuts at: 0 711 872 2058 2292 2763
Size: 711 161 1186 234 471
Fragments arranged by size:
1186 711 471 234 161
NcoI C'CATG_G
Cuts at: 0 759 2763
Size: 759 2004
NsiI A_TGCA'T
Cuts at: 0 2177 2478 2763
Size: 2177 301 285
Fragments arranged by size:
2177 301 285
NspI r_CATG'y
Cuts at: 0 1377 1909 2179 2763
Size: 1377 532 270 584
Fragments arranged by size:
1377 584 532 270
PasI CC'CwG_GG
Cuts at: 0 262 363 511 1979 2763
Size: 262 101 148 1468 784
Fragments arranged by size:
1468 784 262 148 101
PciI A'CATG_T
Cuts at: 0 1373 2763
Size: 1373 1390
PflMI CCAn_nnn'nTGG
Cuts at: 0 2274 2763
Size: 2274 489
PfoI T'CCnGG_A
Cuts at: 0 1961 2507 2763
Size: 1961 546 256
Fragments arranged by size:
1961 546 256
PmlI CAC'GTG
Cuts at: 0 1820 2763
Size: 1820 943
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2290 2322 2763
Size: 2290 32 441
Fragments arranged by size:
2290 441 32
PpuMI rG'GwC_Cy
Cuts at: 0 138 1229 2452 2763
Size: 138 1091 1223 311
Fragments arranged by size:
1223 1091 311 138
PsiI TTA'TAA
Cuts at: 0 1270 2763
Size: 1270 1493
PspOMI G'GGCC_C
Cuts at: 0 846 1198 1975 2259 2763
Size: 846 352 777 284 504
Fragments arranged by size:
846 777 504 352 284
PspXI vC'TCGA_Gb
Cuts at: 0 313 2763
Size: 313 2450
PstI C_TGCA'G
Cuts at: 0 1043 1346 1465 2763
Size: 1043 303 119 1298
Fragments arranged by size:
1298 1043 303 119
PvuII CAG'CTG
Cuts at: 0 711 2763
Size: 711 2052
SacI G_AGCT'C
Cuts at: 0 2222 2763
Size: 2222 541
SanDI GG'GwC_CC
Cuts at: 0 2452 2763
Size: 2452 311
SapI GCTCTTCn'nnn_
Cuts at: 0 261 644 2763
Size: 261 383 2119
Fragments arranged by size:
2119 383 261
ScaI AGT'ACT
Cuts at: 0 1638 2763
Size: 1638 1125
SfcI C'TryA_G
Cuts at: 0 1039 1342 1461 2763
Size: 1039 303 119 1302
Fragments arranged by size:
1302 1039 303 119
SmlI C'TyrA_G
Cuts at: 0 313 2763
Size: 313 2450
SphI G_CATG'C
Cuts at: 0 1909 2179 2763
Size: 1909 270 584
Fragments arranged by size:
1909 584 270
SspI AAT'ATT
Cuts at: 0 2397 2727 2763
Size: 2397 330 36
Fragments arranged by size:
2397 330 36
StuI AGG'CCT
Cuts at: 0 2659 2763
Size: 2659 104
StyI C'CwwG_G
Cuts at: 0 759 1250 2246 2763
Size: 759 491 996 517
Fragments arranged by size:
996 759 517 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 299 2763
Size: 299 2464
TatI w'GTAC_w
Cuts at: 0 279 1547 1636 2552 2763
Size: 279 1268 89 916 211
Fragments arranged by size:
1268 916 279 211 89
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1228 1260 2763
Size: 1228 32 1503
Fragments arranged by size:
1503 1228 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2515 2763
Size: 2515 248
XhoI C'TCGA_G
Cuts at: 0 313 2763
Size: 313 2450
XmnI GAAnn'nnTTC
Cuts at: 0 86 494 653 2763
Size: 86 408 159 2110
Fragments arranged by size:
2110 408 159 86
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AloI ApaI
AvaI BanI BanII BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV Hin4I HincII HindIII KpnI MmeI MscI
MslI MspA1I NcoI NsiI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PsiI PspOMI PspXI PstI
PvuII SacI SanDI SapI ScaI SfcI SmlI SphI
SspI StuI StyI TaqII TatI TstI XcmI XhoI
XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AhdI AleI AlfI AlwNI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BclI BmtI BpuEI BsaBI
BsaHI BsiWI BspEI BssHII BstAPI BstBI BstEII BstZ17I
BtgZI DrdI EagI EcoRI FalI FseI FspI FspAI
HaeII HpaI KasI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PmeI PshAI PsrI
PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I
XbaI XmaI ZraI