Restriction map for
pF1KA0739
(Linear) (Six-base) MAPSORT of: KIAA0739.seq Check: 8941 from: 1 to: 3213
KIAA0739.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1979 3213
Size: 1979 1234
AccI GT'mk_AC
Cuts at: 0 938 3185 3213
Size: 938 2247 28
Fragments arranged by size:
2247 938 28
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1399 1880 3213
Size: 520 879 481 1333
Fragments arranged by size:
1333 879 520 481
AflIII A'CryG_T
Cuts at: 0 2637 3213
Size: 2637 576
AleI CACnn'nnGTG
Cuts at: 0 2302 2638 3213
Size: 2302 336 575
Fragments arranged by size:
2302 575 336
ApaLI G'TGCA_C
Cuts at: 0 2935 3213
Size: 2935 278
ApoI r'AATT_y
Cuts at: 0 647 959 1729 2843 3213
Size: 647 312 770 1114 370
Fragments arranged by size:
1114 770 647 370 312
AseI AT'TA_AT
Cuts at: 0 602 2396 3213
Size: 602 1794 817
Fragments arranged by size:
1794 817 602
BamHI G'GATC_C
Cuts at: 0 1284 3213
Size: 1284 1929
BanI G'GyrC_C
Cuts at: 0 391 3213
Size: 391 2822
BbsI GAAGACnn'nnnn_
Cuts at: 0 2751 3213
Size: 2751 462
BbvCI CC'TCA_GC
Cuts at: 0 797 3213
Size: 797 2416
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 244 278 3213
Size: 244 34 2935
Fragments arranged by size:
2935 244 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 58 3213
Size: 58 3155
BfrBI ATG'CAT
Cuts at: 0 627 2106 3213
Size: 627 1479 1107
Fragments arranged by size:
1479 1107 627
BglII A'GATC_T
Cuts at: 0 446 3213
Size: 446 2767
BlpI GC'TnA_GC
Cuts at: 0 3082 3213
Size: 3082 131
Bme1580I G_kGCm'C
Cuts at: 0 2939 3213
Size: 2939 274
BmrI ACTGGGnnnn_n'
Cuts at: 0 977 1433 1649 2079 2348 3213
Size: 977 456 216 430 269 865
Fragments arranged by size:
977 865 456 430 269 216
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1061 1240 1516 3213
Size: 1061 179 276 1697
Fragments arranged by size:
1697 1061 276 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 1031 1256 1367 1500 2011 2699 2981
Size: 558 473 225 111 133 511 688 282
Cuts at: 2981 3213
Size: 232
Fragments arranged by size:
688 558 511 473 282 232 225 133
111
Bpu10I CC'TnA_GC
Cuts at: 0 586 797 1972 3213
Size: 586 211 1175 1241
Fragments arranged by size:
1241 1175 586 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 2190 2509 2635 3213
Size: 79 2111 319 126 578
Fragments arranged by size:
2111 578 319 126 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 50 1927 3213
Size: 50 1877 1286
Fragments arranged by size:
1877 1286 50
BsaWI w'CCGG_w
Cuts at: 0 293 3213
Size: 293 2920
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 840 870 3213
Size: 840 30 2343
Fragments arranged by size:
2343 840 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 29 848 3213
Size: 29 819 2365
Fragments arranged by size:
2365 819 29
BseYI C'CCAG_C
Cuts at: 0 2445 2574 3213
Size: 2445 129 639
Fragments arranged by size:
2445 639 129
BsiHKAI G_wGCw'C
Cuts at: 0 1268 2939 3213
Size: 1268 1671 274
Fragments arranged by size:
1671 1268 274
BsmI GAATG_Cn'
Cuts at: 0 2098 2671 3213
Size: 2098 573 542
Fragments arranged by size:
2098 573 542
BsmBI CGTCTCn'nnnn_
Cuts at: 0 291 3213
Size: 291 2922
Bsp1286I G_dGCh'C
Cuts at: 0 1268 2939 3213
Size: 1268 1671 274
Fragments arranged by size:
1671 1268 274
BspHI T'CATG_A
Cuts at: 0 420 950 1169 1188 2762 3213
Size: 420 530 219 19 1574 451
Fragments arranged by size:
1574 530 451 420 219 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1979 2918 3213
Size: 1979 939 295
Fragments arranged by size:
1979 939 295
BsrDI GCAATG_nn'
Cuts at: 0 565 3213
Size: 565 2648
BsrFI r'CCGG_y
Cuts at: 0 251 3213
Size: 251 2962
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1822 3213
Size: 1822 1391
BstXI CCAn_nnnn'nTGG
Cuts at: 0 990 1147 1280 2574 2697 3213
Size: 990 157 133 1294 123 516
Fragments arranged by size:
1294 990 516 157 133 123
BstYI r'GATC_y
Cuts at: 0 446 1153 1284 2122 3057 3213
Size: 446 707 131 838 935 156
Fragments arranged by size:
935 838 707 446 156 131
BstZ17I GTA'TAC
Cuts at: 0 3186 3213
Size: 3186 27
BtgI C'CryG_G
Cuts at: 0 1157 2261 3213
Size: 1157 1104 952
Fragments arranged by size:
1157 1104 952
EaeI y'GGCC_r
Cuts at: 0 249 282 1160 2133 2564 3213
Size: 249 33 878 973 431 649
Fragments arranged by size:
973 878 649 431 249 33
EarI CTCTTCn'nnn_
Cuts at: 0 194 401 754 2456 2947 3152 3213
Size: 194 207 353 1702 491 205 61
Fragments arranged by size:
1702 491 353 207 205 194 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 558 1031 1256 1367 1399 1500 1880
Size: 520 38 473 225 111 32 101 380
Cuts at: 1880 2011 2699 2981 3213
Size: 131 688 282 232
Fragments arranged by size:
688 520 473 380 282 232 225 131
111 101 38 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1035 1252 3213
Size: 1035 217 1961
Fragments arranged by size:
1961 1035 217
EcoO109I rG'GnC_Cy
Cuts at: 0 1467 3194 3213
Size: 1467 1727 19
Fragments arranged by size:
1727 1467 19
EcoRI G'AATT_C
Cuts at: 0 2843 3213
Size: 2843 370
FspI TGC'GCA
Cuts at: 0 2929 3213
Size: 2929 284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 349 381 651 683 1658 1690 2382 2414
Size: 349 32 270 32 975 32 692 32
Cuts at: 2414 3213
Size: 799
Fragments arranged by size:
975 799 692 349 270 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2960 3213
Size: 2960 253
HindIII A'AGCT_T
Cuts at: 0 2341 3213
Size: 2341 872
MfeI C'AATT_G
Cuts at: 0 1610 3213
Size: 1610 1603
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 975 3107 3213
Size: 895 80 2132 106
Fragments arranged by size:
2132 895 106 80
MscI TGG'CCA
Cuts at: 0 284 1162 2566 3213
Size: 284 878 1404 647
Fragments arranged by size:
1404 878 647 284
MslI CAynn'nnrTG
Cuts at: 0 749 1168 1948 2302 2638 3213
Size: 749 419 780 354 336 575
Fragments arranged by size:
780 749 575 419 354 336
MspA1I CmG'CkG
Cuts at: 0 1051 1962 2925 3001 3213
Size: 1051 911 963 76 212
Fragments arranged by size:
1051 963 911 212 76
NaeI GCC'GGC
Cuts at: 0 253 3213
Size: 253 2960
NcoI C'CATG_G
Cuts at: 0 1157 2261 3213
Size: 1157 1104 952
Fragments arranged by size:
1157 1104 952
NgoMIV G'CCGG_C
Cuts at: 0 251 3213
Size: 251 2962
NsiI A_TGCA'T
Cuts at: 0 629 2108 3213
Size: 629 1479 1105
Fragments arranged by size:
1479 1105 629
NspI r_CATG'y
Cuts at: 0 415 631 1551 1953 2641 2920 3213
Size: 415 216 920 402 688 279 293
Fragments arranged by size:
920 688 415 402 293 279 216
PciI A'CATG_T
Cuts at: 0 2637 3213
Size: 2637 576
PflMI CCAn_nnn'nTGG
Cuts at: 0 2417 3213
Size: 2417 796
PfoI T'CCnGG_A
Cuts at: 0 617 662 1272 2676 3213
Size: 617 45 610 1404 537
Fragments arranged by size:
1404 617 610 537 45
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2507 2539 3213
Size: 2507 32 674
Fragments arranged by size:
2507 674 32
PstI C_TGCA'G
Cuts at: 0 1990 3213
Size: 1990 1223
PvuII CAG'CTG
Cuts at: 0 1051 1962 3001 3213
Size: 1051 911 1039 212
Fragments arranged by size:
1051 1039 911 212
SapI GCTCTTCn'nnn_
Cuts at: 0 194 401 2456 3213
Size: 194 207 2055 757
Fragments arranged by size:
2055 757 207 194
ScaI AGT'ACT
Cuts at: 0 1692 2032 2798 3213
Size: 1692 340 766 415
Fragments arranged by size:
1692 766 415 340
SexAI A'CCwGG_T
Cuts at: 0 1253 3213
Size: 1253 1960
SfcI C'TryA_G
Cuts at: 0 1986 2836 3213
Size: 1986 850 377
Fragments arranged by size:
1986 850 377
SmlI C'TyrA_G
Cuts at: 0 94 2205 2524 2650 3213
Size: 94 2111 319 126 563
Fragments arranged by size:
2111 563 319 126 94
SphI G_CATG'C
Cuts at: 0 631 3213
Size: 631 2582
StuI AGG'CCT
Cuts at: 0 338 979 1067 2726 3213
Size: 338 641 88 1659 487
Fragments arranged by size:
1659 641 487 338 88
StyI C'CwwG_G
Cuts at: 0 1157 2261 3032 3149 3213
Size: 1157 1104 771 117 64
Fragments arranged by size:
1157 1104 771 117 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1323 2566 3213
Size: 1323 1243 647
Fragments arranged by size:
1323 1243 647
TatI w'GTAC_w
Cuts at: 0 169 1535 1690 1993 2030 2458 2796 3213
Size: 169 1366 155 303 37 428 338 417
Fragments arranged by size:
1366 428 417 338 303 169 155 37
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2104 3213
Size: 2104 1109
XmnI GAAnn'nnTTC
Cuts at: 0 725 3213
Size: 725 2488
Enzymes that do cut:
AarI AccI AcuI AflIII AleI ApaLI ApoI AseI
BamHI BanI BbsI BbvCI BcgI BciVI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BstAPI BstXI BstYI BstZ17I
BtgI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FspI
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PpiI PstI PvuII SapI ScaI SexAI SfcI SmlI
SphI StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI AscI AsiSI AvaI AvrII BaeI
BanII BbeI BclI BglI BmgBI BmtI BsaAI BsaBI
BsaHI BsgI BsiEI BsiWI BspEI BsrBI BsrGI BssHII
BssSI BstBI BstEII Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoRV FalI
FseI FspAI HaeII HpaI KasI KpnI MluI NarI
NdeI NheI NotI NruI PacI PasI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TstI
Tth111I XbaI XhoI XmaI ZraI