Restriction map for
pF1KA0740
(Linear) (Six-base) MAPSORT of: KIAA0740.seq Check: 8901 from: 1 to: 2088
KIAA0740.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 647 2088
Size: 647 1441
AccI GT'mk_AC
Cuts at: 0 980 2088
Size: 980 1108
Acc65I G'GTAC_C
Cuts at: 0 1964 2088
Size: 1964 124
AclI AA'CG_TT
Cuts at: 0 32 1447 2088
Size: 32 1415 641
Fragments arranged by size:
1415 641 32
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 799 1196 1990 2088
Size: 670 129 397 794 98
Fragments arranged by size:
794 670 397 129 98
AfeI AGC'GCT
Cuts at: 0 620 2088
Size: 620 1468
AgeI A'CCGG_T
Cuts at: 0 720 2088
Size: 720 1368
AleI CACnn'nnGTG
Cuts at: 0 145 2088
Size: 145 1943
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 655 2088
Size: 655 1433
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1096 2088
Size: 135 961 992
Fragments arranged by size:
992 961 135
ApaI G_GGCC'C
Cuts at: 0 1009 1187 2088
Size: 1009 178 901
Fragments arranged by size:
1009 901 178
ApoI r'AATT_y
Cuts at: 0 731 845 871 2067 2088
Size: 731 114 26 1196 21
Fragments arranged by size:
1196 731 114 26 21
AseI AT'TA_AT
Cuts at: 0 1675 2088
Size: 1675 413
AvaI C'yCGr_G
Cuts at: 0 206 1318 2088
Size: 206 1112 770
Fragments arranged by size:
1112 770 206
AvrII C'CTAG_G
Cuts at: 0 1012 2088
Size: 1012 1076
BanI G'GyrC_C
Cuts at: 0 416 467 635 690 1943 1964 2088
Size: 416 51 168 55 1253 21 124
Fragments arranged by size:
1253 416 168 124 55 51 21
BanII G_rGCy'C
Cuts at: 0 1009 1187 2088
Size: 1009 178 901
Fragments arranged by size:
1009 901 178
BbeI G_GCGC'C
Cuts at: 0 471 2088
Size: 471 1617
BbsI GAAGACnn'nnnn_
Cuts at: 0 85 1034 2088
Size: 85 949 1054
Fragments arranged by size:
1054 949 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2039 2073 2088
Size: 2039 34 15
Fragments arranged by size:
2039 34 15
BciVI GTATCCnnnnn_n'
Cuts at: 0 243 377 2088
Size: 243 134 1711
Fragments arranged by size:
1711 243 134
BclI T'GATC_A
Cuts at: 0 261 2088
Size: 261 1827
Bme1580I G_kGCm'C
Cuts at: 0 1009 1187 1494 2088
Size: 1009 178 307 594
Fragments arranged by size:
1009 594 307 178
BmgBI CAC'GTC
Cuts at: 0 1458 2088
Size: 1458 630
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 251 1807 2088
Size: 251 1556 281
Fragments arranged by size:
1556 281 251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1228 2060 2088
Size: 1228 832 28
Fragments arranged by size:
1228 832 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2028 2088
Size: 2028 60
BsaAI yAC'GTr
Cuts at: 0 147 1404 2088
Size: 147 1257 684
Fragments arranged by size:
1257 684 147
BsaHI Gr'CG_yC
Cuts at: 0 468 2088
Size: 468 1620
BsaWI w'CCGG_w
Cuts at: 0 720 1114 2088
Size: 720 394 974
Fragments arranged by size:
974 720 394
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1015 2088
Size: 1015 1073
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1109 1845 2088
Size: 1109 736 243
Fragments arranged by size:
1109 736 243
BsiHKAI G_wGCw'C
Cuts at: 0 1793 2088
Size: 1793 295
Bsp1286I G_dGCh'C
Cuts at: 0 1009 1187 1494 1793 2088
Size: 1009 178 307 299 295
Fragments arranged by size:
1009 307 299 295 178
BspHI T'CATG_A
Cuts at: 0 1355 1373 2088
Size: 1355 18 715
Fragments arranged by size:
1355 715 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 284 647 2088
Size: 284 363 1441
Fragments arranged by size:
1441 363 284
BsrBI CCG'CTC
Cuts at: 0 1941 2088
Size: 1941 147
BsrFI r'CCGG_y
Cuts at: 0 720 2088
Size: 720 1368
BssSI C'ACGA_G
Cuts at: 0 1761 2088
Size: 1761 327
BstXI CCAn_nnnn'nTGG
Cuts at: 0 158 1194 2088
Size: 158 1036 894
Fragments arranged by size:
1036 894 158
BstYI r'GATC_y
Cuts at: 0 1656 2088
Size: 1656 432
Bsu36I CC'TnA_GG
Cuts at: 0 1022 2088
Size: 1022 1066
BtgI C'CryG_G
Cuts at: 0 71 749 2088
Size: 71 678 1339
Fragments arranged by size:
1339 678 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2023 2088
Size: 2023 65
BtsI GCAGTG_nn'
Cuts at: 0 2087 2088
Size: 2087 1
DraI TTT'AAA
Cuts at: 0 1467 2088
Size: 1467 621
DrdI GACnn_nn'nnGTC
Cuts at: 0 1213 2088
Size: 1213 875
EaeI y'GGCC_r
Cuts at: 0 137 1835 2088
Size: 137 1698 253
Fragments arranged by size:
1698 253 137
EarI CTCTTCn'nnn_
Cuts at: 0 867 2088
Size: 867 1221
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 981 2088
Size: 981 1107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 799 1196 1228 1990 2060 2088
Size: 670 129 397 32 762 70 28
Fragments arranged by size:
762 670 397 129 70 32 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1060 2088
Size: 1060 1028
EcoO109I rG'GnC_Cy
Cuts at: 0 1005 1183 1225 1314 2088
Size: 1005 178 42 89 774
Fragments arranged by size:
1005 774 178 89 42
EcoRI G'AATT_C
Cuts at: 0 2067 2088
Size: 2067 21
EcoRV GAT'ATC
Cuts at: 0 1601 2088
Size: 1601 487
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 102 1386 2076 2088
Size: 102 1284 690 12
Fragments arranged by size:
1284 690 102 12
HaeII r_GCGC'y
Cuts at: 0 471 622 2088
Size: 471 151 1466
Fragments arranged by size:
1466 471 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 315 347 2088
Size: 315 32 1741
Fragments arranged by size:
1741 315 32
HincII GTy'rAC
Cuts at: 0 981 1161 1748 2088
Size: 981 180 587 340
Fragments arranged by size:
981 587 340 180
KasI G'GCGC_C
Cuts at: 0 467 2088
Size: 467 1621
KpnI G_GTAC'C
Cuts at: 0 1968 2088
Size: 1968 120
MscI TGG'CCA
Cuts at: 0 139 2088
Size: 139 1949
MslI CAynn'nnrTG
Cuts at: 0 145 1138 1192 2088
Size: 145 993 54 896
Fragments arranged by size:
993 896 145 54
MspA1I CmG'CkG
Cuts at: 0 132 1503 1668 1949 2088
Size: 132 1371 165 281 139
Fragments arranged by size:
1371 281 165 139 132
NarI GG'CG_CC
Cuts at: 0 468 2088
Size: 468 1620
NcoI C'CATG_G
Cuts at: 0 749 2088
Size: 749 1339
NdeI CA'TA_TG
Cuts at: 0 288 2088
Size: 288 1800
NspI r_CATG'y
Cuts at: 0 363 1143 1735 2088
Size: 363 780 592 353
Fragments arranged by size:
780 592 363 353
PflMI CCAn_nnn'nTGG
Cuts at: 0 1833 2088
Size: 1833 255
PmlI CAC'GTG
Cuts at: 0 147 2088
Size: 147 1941
PpuMI rG'GwC_Cy
Cuts at: 0 1314 2088
Size: 1314 774
PshAI GACnn'nnGTC
Cuts at: 0 1195 2088
Size: 1195 893
PspOMI G'GGCC_C
Cuts at: 0 1005 1183 2088
Size: 1005 178 905
Fragments arranged by size:
1005 905 178
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 530 562 2088
Size: 530 32 1526
Fragments arranged by size:
1526 530 32
PstI C_TGCA'G
Cuts at: 0 1920 2088
Size: 1920 168
PvuII CAG'CTG
Cuts at: 0 132 2088
Size: 132 1956
SalI G'TCGA_C
Cuts at: 0 979 2088
Size: 979 1109
SfcI C'TryA_G
Cuts at: 0 1512 1916 2088
Size: 1512 404 172
Fragments arranged by size:
1512 404 172
SfoI GGC'GCC
Cuts at: 0 469 2088
Size: 469 1619
SmlI C'TyrA_G
Cuts at: 0 1318 2043 2088
Size: 1318 725 45
Fragments arranged by size:
1318 725 45
SphI G_CATG'C
Cuts at: 0 1143 1735 2088
Size: 1143 592 353
Fragments arranged by size:
1143 592 353
StyI C'CwwG_G
Cuts at: 0 749 1012 1682 2088
Size: 749 263 670 406
Fragments arranged by size:
749 670 406 263
SwaI ATTT'AAAT
Cuts at: 0 1467 2088
Size: 1467 621
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 49 157 404 2088
Size: 49 108 247 1684
Fragments arranged by size:
1684 247 108 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 389 421 960 992 2088
Size: 389 32 539 32 1096
Fragments arranged by size:
1096 539 389 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1767 2088
Size: 1767 321
XhoI C'TCGA_G
Cuts at: 0 1318 2088
Size: 1318 770
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AgeI AleI
AlfI AlwNI ApaI ApoI AseI AvaI AvrII BanI
BanII BbeI BbsI BcgI BciVI BclI Bme1580I BmgBI
BplI BpmI BpuEI BsaAI BsaHI BsaWI BseRI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I
NarI NcoI NdeI NspI PflMI PmlI PpuMI PshAI
PspOMI PsrI PstI PvuII SalI SfcI SfoI SmlI
SphI StyI SwaI TaqII TstI XcmI XhoI
Enzymes that do not cut:
AatII AflII AflIII AhdI AloI ApaLI AscI AsiSI
BaeI BamHI BbvCI BfrBI BglI BglII BlpI BmrI
BmtI Bpu10I BsaI BsaBI BsaXI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I ClaI CspCI DraIII EagI EcoICRI FseI
FspI FspAI HindIII HpaI MfeI MluI MmeI NaeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PpiI PsiI PspXI PvuI RsrII SacI
SacII SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI TatI
Tth111I XbaI XmaI XmnI ZraI