Restriction map for
pF1KA0743
(Linear) (Six-base) MAPSORT of: KIAA0743.seq Check: 5911 from: 1 to: 3183
KIAA0743.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2044 2448 3183
Size: 2044 404 735
Fragments arranged by size:
2044 735 404
AccI GT'mk_AC
Cuts at: 0 395 2728 3183
Size: 395 2333 455
Fragments arranged by size:
2333 455 395
Acc65I G'GTAC_C
Cuts at: 0 506 3183
Size: 506 2677
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1477 1525 2229 3183
Size: 1477 48 704 954
Fragments arranged by size:
1477 954 704 48
AflII C'TTAA_G
Cuts at: 0 1243 3183
Size: 1243 1940
AflIII A'CryG_T
Cuts at: 0 617 3183
Size: 617 2566
AhdI GACnn_n'nnGTC
Cuts at: 0 3045 3183
Size: 3045 138
AleI CACnn'nnGTG
Cuts at: 0 1801 2254 3183
Size: 1801 453 929
Fragments arranged by size:
1801 929 453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 791 3183
Size: 791 2392
AvaI C'yCGr_G
Cuts at: 0 1760 1913 3183
Size: 1760 153 1270
Fragments arranged by size:
1760 1270 153
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1268 1301 3183
Size: 1268 33 1882
Fragments arranged by size:
1882 1268 33
BanI G'GyrC_C
Cuts at: 0 457 506 892 1825 3183
Size: 457 49 386 933 1358
Fragments arranged by size:
1358 933 457 386 49
BanII G_rGCy'C
Cuts at: 0 10 289 320 454 2588 3133 3183
Size: 10 279 31 134 2134 545 50
Fragments arranged by size:
2134 545 279 134 50 31 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 2340 2861 3183
Size: 2340 521 322
Fragments arranged by size:
2340 521 322
BbvCI CC'TCA_GC
Cuts at: 0 1271 3183
Size: 1271 1912
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1198 1232 2694 2728 3183
Size: 1198 34 1462 34 455
Fragments arranged by size:
1462 1198 455 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 892 3183
Size: 892 2291
BclI T'GATC_A
Cuts at: 0 1700 3183
Size: 1700 1483
BfrBI ATG'CAT
Cuts at: 0 987 3183
Size: 987 2196
BmrI ACTGGGnnnn_n'
Cuts at: 0 912 2021 3183
Size: 912 1109 1162
Fragments arranged by size:
1162 1109 912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1044 1108 1137 1350 2276 2296 3183
Size: 151 893 64 29 213 926 20 887
Fragments arranged by size:
926 893 887 213 151 64 29 20
Bpu10I CC'TnA_GC
Cuts at: 0 937 1271 1525 3183
Size: 937 334 254 1658
Fragments arranged by size:
1658 937 334 254
BsaI GGTCTCn'nnnn_
Cuts at: 0 229 1162 1283 3183
Size: 229 933 121 1900
Fragments arranged by size:
1900 933 229 121
BsaAI yAC'GTr
Cuts at: 0 702 2153 3180 3183
Size: 702 1451 1027 3
Fragments arranged by size:
1451 1027 702 3
BsaBI GATnn'nnATC
Cuts at: 0 172 3183
Size: 172 3011
BsaHI Gr'CG_yC
Cuts at: 0 1959 2559 3183
Size: 1959 600 624
Fragments arranged by size:
1959 624 600
BsaWI w'CCGG_w
Cuts at: 0 895 3183
Size: 895 2288
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1135 1165 3183
Size: 1135 30 2018
Fragments arranged by size:
2018 1135 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 406 2398 3183
Size: 406 1992 785
Fragments arranged by size:
1992 785 406
BseYI C'CCAG_C
Cuts at: 0 3 1025 2212 3183
Size: 3 1022 1187 971
Fragments arranged by size:
1187 1022 971 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 870 3183
Size: 870 2313
BsiHKAI G_wGCw'C
Cuts at: 0 2292 3133 3183
Size: 2292 841 50
Fragments arranged by size:
2292 841 50
BsmI GAATG_Cn'
Cuts at: 0 777 2798 3183
Size: 777 2021 385
Fragments arranged by size:
2021 777 385
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3058 3183
Size: 3058 125
Bsp1286I G_dGCh'C
Cuts at: 0 10 289 320 454 2292 2588 3133 3183
Size: 10 279 31 134 1838 296 545 50
Fragments arranged by size:
1838 545 296 279 134 50 31 10
BspHI T'CATG_A
Cuts at: 0 1331 3110 3183
Size: 1331 1779 73
Fragments arranged by size:
1779 1331 73
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 922 2044 2448 3183
Size: 922 1122 404 735
Fragments arranged by size:
1122 922 735 404
BsrBI CCG'CTC
Cuts at: 0 1997 3140 3183
Size: 1997 1143 43
Fragments arranged by size:
1997 1143 43
BsrDI GCAATG_nn'
Cuts at: 0 1632 1634 2142 3183
Size: 1632 2 508 1041
Fragments arranged by size:
1632 1041 508 2
BsrFI r'CCGG_y
Cuts at: 0 889 2498 3183
Size: 889 1609 685
Fragments arranged by size:
1609 889 685
BsrGI T'GTAC_A
Cuts at: 0 956 1883 3020 3183
Size: 956 927 1137 163
Fragments arranged by size:
1137 956 927 163
BssSI C'ACGA_G
Cuts at: 0 1903 3183
Size: 1903 1280
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1836 2581 3183
Size: 1836 745 602
Fragments arranged by size:
1836 745 602
BstYI r'GATC_y
Cuts at: 0 596 3183
Size: 596 2587
BtgI C'CryG_G
Cuts at: 0 407 977 1253 2237 2891 3183
Size: 407 570 276 984 654 292
Fragments arranged by size:
984 654 570 407 292 276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1470 3183
Size: 1470 1713
BtsI GCAGTG_nn'
Cuts at: 0 823 3183
Size: 823 2360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1886 1921 3183
Size: 1886 35 1262
Fragments arranged by size:
1886 1262 35
EaeI y'GGCC_r
Cuts at: 0 209 1377 2194 2470 3141 3183
Size: 209 1168 817 276 671 42
Fragments arranged by size:
1168 817 671 276 209 42
EarI CTCTTCn'nnn_
Cuts at: 0 340 1544 1570 1612 1991 2569 3130 3183
Size: 340 1204 26 42 379 578 561 53
Fragments arranged by size:
1204 578 561 379 340 53 42 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3078 3183
Size: 3078 105
EcoICRI GAG'CTC
Cuts at: 0 3131 3183
Size: 3131 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1044 1108 1137 1350 1477 1525 2229
Size: 151 893 64 29 213 127 48 704
Cuts at: 2229 2276 2296 3183
Size: 47 20 887
Fragments arranged by size:
893 887 704 213 151 127 64 48
47 29 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 97 918 3183
Size: 97 821 2265
Fragments arranged by size:
2265 821 97
EcoO109I rG'GnC_Cy
Cuts at: 0 2023 2921 3047 3183
Size: 2023 898 126 136
Fragments arranged by size:
2023 898 136 126
HaeII r_GCGC'y
Cuts at: 0 2150 3183
Size: 2150 1033
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 520 552 991 1023 3031 3063
Size: 436 32 52 32 439 32 2008 32
Cuts at: 3063 3183
Size: 120
Fragments arranged by size:
2008 439 436 120 52 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1122 1250 2367 2507 3183
Size: 1122 128 1117 140 676
Fragments arranged by size:
1122 1117 676 140 128
HpaI GTT'AAC
Cuts at: 0 1122 1250 2507 3183
Size: 1122 128 1257 676
Fragments arranged by size:
1257 1122 676 128
KpnI G_GTAC'C
Cuts at: 0 510 3183
Size: 510 2673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2649 3183
Size: 2649 534
MscI TGG'CCA
Cuts at: 0 211 1379 2196 2472 3183
Size: 211 1168 817 276 711
Fragments arranged by size:
1168 817 711 276 211
MslI CAynn'nnrTG
Cuts at: 0 166 466 958 976 982 1555 1801 2254
Size: 166 300 492 18 6 573 246 453
Cuts at: 2254 3109 3183
Size: 855 74
Fragments arranged by size:
855 573 492 453 300 246 166 74
18 6
MspA1I CmG'CkG
Cuts at: 0 49 756 3183
Size: 49 707 2427
Fragments arranged by size:
2427 707 49
NaeI GCC'GGC
Cuts at: 0 2500 3183
Size: 2500 683
NcoI C'CATG_G
Cuts at: 0 977 3183
Size: 977 2206
NgoMIV G'CCGG_C
Cuts at: 0 2498 3183
Size: 2498 685
NsiI A_TGCA'T
Cuts at: 0 989 3183
Size: 989 2194
NspI r_CATG'y
Cuts at: 0 621 957 1884 2076 3183
Size: 621 336 927 192 1107
Fragments arranged by size:
1107 927 621 336 192
PciI A'CATG_T
Cuts at: 0 617 3183
Size: 617 2566
PflMI CCAn_nnn'nTGG
Cuts at: 0 2299 3183
Size: 2299 884
PfoI T'CCnGG_A
Cuts at: 0 599 2292 2936 3183
Size: 599 1693 644 247
Fragments arranged by size:
1693 644 599 247
PpuMI rG'GwC_Cy
Cuts at: 0 2023 3047 3183
Size: 2023 1024 136
Fragments arranged by size:
2023 1024 136
PshAI GACnn'nnGTC
Cuts at: 0 125 3183
Size: 125 3058
PsiI TTA'TAA
Cuts at: 0 110 3183
Size: 110 3073
PstI C_TGCA'G
Cuts at: 0 1388 2459 3183
Size: 1388 1071 724
Fragments arranged by size:
1388 1071 724
PvuII CAG'CTG
Cuts at: 0 756 3183
Size: 756 2427
SacI G_AGCT'C
Cuts at: 0 3133 3183
Size: 3133 50
SapI GCTCTTCn'nnn_
Cuts at: 0 1991 3130 3183
Size: 1991 1139 53
Fragments arranged by size:
1991 1139 53
SbfI CC_TGCA'GG
Cuts at: 0 2459 3183
Size: 2459 724
ScaI AGT'ACT
Cuts at: 0 2609 3183
Size: 2609 574
SfcI C'TryA_G
Cuts at: 0 1384 2455 2492 2789 3183
Size: 1384 1071 37 297 394
Fragments arranged by size:
1384 1071 394 297 37
SmlI C'TyrA_G
Cuts at: 0 1243 1913 3183
Size: 1243 670 1270
Fragments arranged by size:
1270 1243 670
SnaBI TAC'GTA
Cuts at: 0 2153 3183
Size: 2153 1030
SphI G_CATG'C
Cuts at: 0 2076 3183
Size: 2076 1107
SspI AAT'ATT
Cuts at: 0 2640 3183
Size: 2640 543
StyI C'CwwG_G
Cuts at: 0 977 1875 2568 3183
Size: 977 898 693 615
Fragments arranged by size:
977 898 693 615
TatI w'GTAC_w
Cuts at: 0 431 956 1883 2607 2878 3020 3026 3183
Size: 431 525 927 724 271 142 6 157
Fragments arranged by size:
927 724 525 431 271 157 142 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2157 2189 3183
Size: 2157 32 994
Fragments arranged by size:
2157 994 32
Tth111I GACn'n_nGTC
Cuts at: 0 1495 3183
Size: 1495 1688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1033 2457 2754 3183
Size: 1033 1424 297 429
Fragments arranged by size:
1424 1033 429 297
XhoI C'TCGA_G
Cuts at: 0 1913 3183
Size: 1913 1270
XmnI GAAnn'nnTTC
Cuts at: 0 871 3183
Size: 871 2312
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AhdI AleI
AlfI AvaI BaeI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BmrI BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstXI BstYI BtgI BtgZI BtsI CspCI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII HpaI KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PpuMI PshAI PsiI PstI PvuII SacI SapI SbfI
ScaI SfcI SmlI SnaBI SphI SspI StyI TatI
TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BamHI BbeI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI BsiEI
BsiWI BspEI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI DraI DraIII DrdI EagI EcoRI EcoRV FalI
FseI FspI FspAI HindIII KasI MfeI MluI NarI
NdeI NheI NotI NruI PacI PasI PmeI PmlI
PpiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI
SrfI StuI SwaI TaqII XbaI XmaI ZraI