Restriction map for
pF1KA0744
(Linear) (Six-base) MAPSORT of: KIAA0744.seq Check: 7168 from: 1 to: 1770
KIAA0744.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
Acc65I G'GTAC_C
Cuts at: 0 1306 1770
Size: 1306 464
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1504 1510 1770
Size: 232 1272 6 260
Fragments arranged by size:
1272 260 232 6
AlwNI CAG_nnn'CTG
Cuts at: 0 189 315 817 1770
Size: 189 126 502 953
Fragments arranged by size:
953 502 189 126
ApoI r'AATT_y
Cuts at: 0 1032 1286 1770
Size: 1032 254 484
Fragments arranged by size:
1032 484 254
BanI G'GyrC_C
Cuts at: 0 1306 1770
Size: 1306 464
BanII G_rGCy'C
Cuts at: 0 59 665 1770
Size: 59 606 1105
Fragments arranged by size:
1105 606 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 1556 1770
Size: 1556 214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1576 1610 1770
Size: 1576 34 160
Fragments arranged by size:
1576 160 34
BclI T'GATC_A
Cuts at: 0 11 1770
Size: 11 1759
BlpI GC'TnA_GC
Cuts at: 0 242 1379 1770
Size: 242 1137 391
Fragments arranged by size:
1137 391 242
BmrI ACTGGGnnnn_n'
Cuts at: 0 818 1341 1624 1770
Size: 818 523 283 146
Fragments arranged by size:
818 523 283 146
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 78 1770
Size: 78 1692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 337 794 1727 1770
Size: 73 264 457 933 43
Fragments arranged by size:
933 457 264 73 43
Bpu10I CC'TnA_GC
Cuts at: 0 1072 1770
Size: 1072 698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1525 1770
Size: 1525 245
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 278 308 1770
Size: 278 30 1462
Fragments arranged by size:
1462 278 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 365 723 1770
Size: 365 358 1047
Fragments arranged by size:
1047 365 358
BseYI C'CCAG_C
Cuts at: 0 1016 1770
Size: 1016 754
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1337 1770
Size: 1337 433
BsiHKAI G_wGCw'C
Cuts at: 0 618 1372 1770
Size: 618 754 398
Fragments arranged by size:
754 618 398
BsmI GAATG_Cn'
Cuts at: 0 923 1770
Size: 923 847
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1054 1770
Size: 1054 716
Bsp1286I G_dGCh'C
Cuts at: 0 59 618 665 1372 1770
Size: 59 559 47 707 398
Fragments arranged by size:
707 559 398 59 47
BspHI T'CATG_A
Cuts at: 0 933 1770
Size: 933 837
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 959 1770
Size: 959 811
BstAPI GCAn_nnn'nTGC
Cuts at: 0 622 1770
Size: 622 1148
BstBI TT'CG_AA
Cuts at: 0 645 1030 1465 1770
Size: 645 385 435 305
Fragments arranged by size:
645 435 385 305
BstYI r'GATC_y
Cuts at: 0 1442 1676 1770
Size: 1442 234 94
Fragments arranged by size:
1442 234 94
Bsu36I CC'TnA_GG
Cuts at: 0 76 88 1770
Size: 76 12 1682
Fragments arranged by size:
1682 76 12
BtgI C'CryG_G
Cuts at: 0 104 1770
Size: 104 1666
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 337 1555 1770
Size: 337 1218 215
Fragments arranged by size:
1218 337 215
BtsI GCAGTG_nn'
Cuts at: 0 416 1013 1599 1770
Size: 416 597 586 171
Fragments arranged by size:
597 586 416 171
DrdI GACnn_nn'nnGTC
Cuts at: 0 118 1770
Size: 118 1652
EarI CTCTTCn'nnn_
Cuts at: 0 1514 1770
Size: 1514 256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 747 1770
Size: 747 1023
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 232 337 794 1504 1510 1727 1770
Size: 73 159 105 457 710 6 217 43
Fragments arranged by size:
710 457 217 159 105 73 43 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 301 380 726 1770
Size: 301 79 346 1044
Fragments arranged by size:
1044 346 301 79
EcoRI G'AATT_C
Cuts at: 0 1032 1770
Size: 1032 738
HaeII r_GCGC'y
Cuts at: 0 1561 1770
Size: 1561 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 498 530 547 579 1097 1129
Size: 16 32 450 32 17 32 518 32
Cuts at: 1129 1770
Size: 641
Fragments arranged by size:
641 518 450 32 32 32 32 17
16
HindIII A'AGCT_T
Cuts at: 0 436 1225 1770
Size: 436 789 545
Fragments arranged by size:
789 545 436
KpnI G_GTAC'C
Cuts at: 0 1310 1770
Size: 1310 460
MfeI C'AATT_G
Cuts at: 0 133 1770
Size: 133 1637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1770
Size: 505 1265
MslI CAynn'nnrTG
Cuts at: 0 1136 1770
Size: 1136 634
MspA1I CmG'CkG
Cuts at: 0 1383 1770
Size: 1383 387
PflMI CCAn_nnn'nTGG
Cuts at: 0 487 524 817 838 1686 1770
Size: 487 37 293 21 848 84
Fragments arranged by size:
848 487 293 84 37 21
PfoI T'CCnGG_A
Cuts at: 0 1679 1743 1770
Size: 1679 64 27
Fragments arranged by size:
1679 64 27
PstI C_TGCA'G
Cuts at: 0 1190 1770
Size: 1190 580
PvuII CAG'CTG
Cuts at: 0 1383 1770
Size: 1383 387
SapI GCTCTTCn'nnn_
Cuts at: 0 1514 1770
Size: 1514 256
SfcI C'TryA_G
Cuts at: 0 1186 1770
Size: 1186 584
SmlI C'TyrA_G
Cuts at: 0 1504 1770
Size: 1504 266
StyI C'CwwG_G
Cuts at: 0 992 1268 1770
Size: 992 276 502
Fragments arranged by size:
992 502 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1176 1770
Size: 1176 594
TatI w'GTAC_w
Cuts at: 0 418 599 1770
Size: 418 181 1171
Fragments arranged by size:
1171 418 181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 990 1770
Size: 990 780
XmnI GAAnn'nnTTC
Cuts at: 0 149 1523 1770
Size: 149 1374 247
Fragments arranged by size:
1374 247 149
Enzymes that do cut:
Acc65I AcuI AlwNI ApoI BanI BanII BbsI BcgI
BclI BlpI BmrI BplI BpmI Bpu10I BpuEI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DrdI EarI EciI Eco57MI EcoNI EcoRI HaeII Hin4I
HindIII KpnI MfeI MmeI MslI MspA1I PflMI PfoI
PstI PvuII SapI SfcI SmlI StyI TaqII TatI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BciVI
BfrBI BglI BglII Bme1580I BmgBI BmtI BsaI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstEII BstXI BstZ17I ClaI
CspCI DraI DraIII EaeI EagI EcoICRI EcoO109I EcoRV
FalI FseI FspI FspAI HincII HpaI KasI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
XmaI ZraI