Restriction map for
pF1KSDA0752
(Linear) (Six-base) MAPSORT of: KIAA0752.seq Check: 1449 from: 1 to: 930
KIAA0752.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 594 696 804 930
Size: 594 102 108 126
Fragments arranged by size:
594 126 108 102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 436 930
Size: 290 146 494
Fragments arranged by size:
494 290 146
AlwNI CAG_nnn'CTG
Cuts at: 0 202 376 459 574 676 930
Size: 202 174 83 115 102 254
Fragments arranged by size:
254 202 174 115 102 83
AvaI C'yCGr_G
Cuts at: 0 172 930
Size: 172 758
BanI G'GyrC_C
Cuts at: 0 316 930
Size: 316 614
BanII G_rGCy'C
Cuts at: 0 65 930
Size: 65 865
BbsI GAAGACnn'nnnn_
Cuts at: 0 112 347 508 545 647 746 930
Size: 112 235 161 37 102 99 184
Fragments arranged by size:
235 184 161 112 102 99 37
BclI T'GATC_A
Cuts at: 0 437 930
Size: 437 493
BglI GCCn_nnn'nGGC
Cuts at: 0 622 724 930
Size: 622 102 206
Fragments arranged by size:
622 206 102
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 198 930
Size: 198 732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 565 667 930
Size: 367 198 102 263
Fragments arranged by size:
367 263 198 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 47 77 130 160 930
Size: 47 30 53 30 770
Fragments arranged by size:
770 53 47 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 280 364 493 496 508 562 664
Size: 259 21 84 129 3 12 54 102
Cuts at: 664 930
Size: 266
Fragments arranged by size:
266 259 129 102 84 54 21 12
3
BseYI C'CCAG_C
Cuts at: 0 612 714 874 930
Size: 612 102 160 56
Fragments arranged by size:
612 160 102 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 930
Size: 776 154
BsiHKAI G_wGCw'C
Cuts at: 0 65 930
Size: 65 865
BsmBI CGTCTCn'nnnn_
Cuts at: 0 181 930
Size: 181 749
Bsp1286I G_dGCh'C
Cuts at: 0 65 930
Size: 65 865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 594 696 804 930
Size: 594 102 108 126
Fragments arranged by size:
594 126 108 102
BsrFI r'CCGG_y
Cuts at: 0 887 930
Size: 887 43
BstEII G'GTnAC_C
Cuts at: 0 331 529 930
Size: 331 198 401
Fragments arranged by size:
401 331 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 115 930
Size: 115 815
DraIII CAC_nnn'GTG
Cuts at: 0 130 526 930
Size: 130 396 404
Fragments arranged by size:
404 396 130
EaeI y'GGCC_r
Cuts at: 0 425 930
Size: 425 505
EarI CTCTTCn'nnn_
Cuts at: 0 824 930
Size: 824 106
EcoICRI GAG'CTC
Cuts at: 0 63 930
Size: 63 867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 367 436 565 667 930
Size: 290 77 69 129 102 263
Fragments arranged by size:
290 263 129 102 77 69
EcoO109I rG'GnC_Cy
Cuts at: 0 411 486 930
Size: 411 75 444
Fragments arranged by size:
444 411 75
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 129 525 930
Size: 129 396 405
Fragments arranged by size:
405 396 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 930
Size: 130 32 768
Fragments arranged by size:
768 130 32
MfeI C'AATT_G
Cuts at: 0 733 930
Size: 733 197
MslI CAynn'nnrTG
Cuts at: 0 31 930
Size: 31 899
NspI r_CATG'y
Cuts at: 0 556 658 930
Size: 556 102 272
Fragments arranged by size:
556 272 102
PflMI CCAn_nnn'nTGG
Cuts at: 0 526 930
Size: 526 404
PpuMI rG'GwC_Cy
Cuts at: 0 411 486 930
Size: 411 75 444
Fragments arranged by size:
444 411 75
PstI C_TGCA'G
Cuts at: 0 801 930
Size: 801 129
SacI G_AGCT'C
Cuts at: 0 65 930
Size: 65 865
SanDI GG'GwC_CC
Cuts at: 0 411 930
Size: 411 519
SfcI C'TryA_G
Cuts at: 0 797 930
Size: 797 133
StyI C'CwwG_G
Cuts at: 0 113 149 509 930
Size: 113 36 360 421
Fragments arranged by size:
421 360 113 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 334 930
Size: 334 596
TatI w'GTAC_w
Cuts at: 0 447 930
Size: 447 483
XbaI T'CTAG_A
Cuts at: 0 240 930
Size: 240 690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 930
Size: 333 597
Enzymes that do cut:
AarI AcuI AlwNI AvaI BanI BanII BbsI BclI
BglI BplI BpmI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrFI BstEII BtgZI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I FalI Hin4I MfeI MslI
NspI PflMI PpuMI PstI SacI SanDI SfcI StyI
TaqII TatI XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XhoI XmaI XmnI ZraI