Restriction map for
pF1KSDA0753
(Linear) (Six-base) MAPSORT of: KIAA0753.seq Check: 2792 from: 1 to: 2901
KIAA0753.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 518 1284 1569 2901
Size: 518 766 285 1332
Fragments arranged by size:
1332 766 518 285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 205 577 2000 2821 2901
Size: 67 138 372 1423 821 80
Fragments arranged by size:
1423 821 372 138 80 67
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1325 2901
Size: 1325 1576
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 743 775 2901
Size: 743 32 2126
Fragments arranged by size:
2126 743 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1807 2866 2901
Size: 1807 1059 35
Fragments arranged by size:
1807 1059 35
ApoI r'AATT_y
Cuts at: 0 564 1166 2881 2901
Size: 564 602 1715 20
Fragments arranged by size:
1715 602 564 20
AvrII C'CTAG_G
Cuts at: 0 43 2901
Size: 43 2858
BamHI G'GATC_C
Cuts at: 0 738 2517 2901
Size: 738 1779 384
Fragments arranged by size:
1779 738 384
BanI G'GyrC_C
Cuts at: 0 508 1527 1662 2901
Size: 508 1019 135 1239
Fragments arranged by size:
1239 1019 508 135
BanII G_rGCy'C
Cuts at: 0 1973 2246 2661 2901
Size: 1973 273 415 240
Fragments arranged by size:
1973 415 273 240
BbeI G_GCGC'C
Cuts at: 0 1531 2901
Size: 1531 1370
BbsI GAAGACnn'nnnn_
Cuts at: 0 336 2506 2901
Size: 336 2170 395
Fragments arranged by size:
2170 395 336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 137 171 769 803 2710 2744 2839 2873
Size: 137 34 598 34 1907 34 95 34
Cuts at: 2873 2901
Size: 28
Fragments arranged by size:
1907 598 137 95 34 34 34 34
28
BciVI GTATCCnnnnn_n'
Cuts at: 0 697 1664 2901
Size: 697 967 1237
Fragments arranged by size:
1237 967 697
BglI GCCn_nnn'nGGC
Cuts at: 0 934 2901
Size: 934 1967
BglII A'GATC_T
Cuts at: 0 549 2267 2319 2901
Size: 549 1718 52 582
Fragments arranged by size:
1718 582 549 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 1015 2901
Size: 1015 1886
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2680 2901
Size: 2680 221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 649 799 1750 2344 2901
Size: 483 166 150 951 594 557
Fragments arranged by size:
951 594 557 483 166 150
Bpu10I CC'TnA_GC
Cuts at: 0 2286 2901
Size: 2286 615
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1766 2901
Size: 1766 1135
BsaI GGTCTCn'nnnn_
Cuts at: 0 1339 2901
Size: 1339 1562
BsaBI GATnn'nnATC
Cuts at: 0 1319 2901
Size: 1319 1582
BsaHI Gr'CG_yC
Cuts at: 0 1528 2901
Size: 1528 1373
BsaWI w'CCGG_w
Cuts at: 0 1341 2901
Size: 1341 1560
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 78 938 1254 2056 2468 2901
Size: 78 860 316 802 412 433
Fragments arranged by size:
860 802 433 412 316 78
BseYI C'CCAG_C
Cuts at: 0 2156 2901
Size: 2156 745
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1621 2147 2901
Size: 1621 526 754
Fragments arranged by size:
1621 754 526
BsiHKAI G_wGCw'C
Cuts at: 0 1973 2246 2661 2901
Size: 1973 273 415 240
Fragments arranged by size:
1973 415 273 240
BsmI GAATG_Cn'
Cuts at: 0 806 2901
Size: 806 2095
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1706 2901
Size: 1706 1195
Bsp1286I G_dGCh'C
Cuts at: 0 1973 2246 2661 2901
Size: 1973 273 415 240
Fragments arranged by size:
1973 415 273 240
BspEI T'CCGG_A
Cuts at: 0 1341 2901
Size: 1341 1560
BspHI T'CATG_A
Cuts at: 0 2330 2742 2901
Size: 2330 412 159
Fragments arranged by size:
2330 412 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 813 2901
Size: 813 2088
BsrDI GCAATG_nn'
Cuts at: 0 902 2901
Size: 902 1999
BsrFI r'CCGG_y
Cuts at: 0 1637 2901
Size: 1637 1264
BstEII G'GTnAC_C
Cuts at: 0 613 2901
Size: 613 2288
BstXI CCAn_nnnn'nTGG
Cuts at: 0 58 2901
Size: 58 2843
BstYI r'GATC_y
Cuts at: 0 549 738 1776 2267 2319 2517 2901
Size: 549 189 1038 491 52 198 384
Fragments arranged by size:
1038 549 491 384 198 189 52
BstZ17I GTA'TAC
Cuts at: 0 519 2901
Size: 519 2382
BtgI C'CryG_G
Cuts at: 0 1454 1666 2767 2901
Size: 1454 212 1101 134
Fragments arranged by size:
1454 1101 212 134
DraIII CAC_nnn'GTG
Cuts at: 0 429 2901
Size: 429 2472
EaeI y'GGCC_r
Cuts at: 0 1213 2901
Size: 1213 1688
EarI CTCTTCn'nnn_
Cuts at: 0 309 695 1871 2342 2621 2789 2901
Size: 309 386 1176 471 279 168 112
Fragments arranged by size:
1176 471 386 309 279 168 112
EcoICRI GAG'CTC
Cuts at: 0 1971 2244 2659 2901
Size: 1971 273 415 242
Fragments arranged by size:
1971 415 273 242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 205 483 577 649 799 1750 2000
Size: 67 138 278 94 72 150 951 250
Cuts at: 2000 2344 2821 2901
Size: 344 477 80
Fragments arranged by size:
951 477 344 278 250 150 138 94
80 72 67
EcoO109I rG'GnC_Cy
Cuts at: 0 47 1244 2546 2610 2901
Size: 47 1197 1302 64 291
Fragments arranged by size:
1302 1197 291 64 47
EcoRI G'AATT_C
Cuts at: 0 2881 2901
Size: 2881 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 304 538 1304 2088 2901
Size: 304 234 766 784 813
Fragments arranged by size:
813 784 766 304 234
HaeII r_GCGC'y
Cuts at: 0 1531 2901
Size: 1531 1370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1169 1201 2119 2151 2176 2208 2493 2525
Size: 1169 32 918 32 25 32 285 32
Cuts at: 2525 2578 2610 2724 2756 2901
Size: 53 32 114 32 145
Fragments arranged by size:
1169 918 285 145 114 53 32 32
32 32 32 32 25
HincII GTy'rAC
Cuts at: 0 1285 2901
Size: 1285 1616
HindIII A'AGCT_T
Cuts at: 0 731 1431 2784 2901
Size: 731 700 1353 117
Fragments arranged by size:
1353 731 700 117
KasI G'GCGC_C
Cuts at: 0 1527 2901
Size: 1527 1374
MfeI C'AATT_G
Cuts at: 0 2002 2901
Size: 2002 899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2022 2219 2901
Size: 2022 197 682
Fragments arranged by size:
2022 682 197
MscI TGG'CCA
Cuts at: 0 1215 2901
Size: 1215 1686
MspA1I CmG'CkG
Cuts at: 0 99 2179 2901
Size: 99 2080 722
Fragments arranged by size:
2080 722 99
NarI GG'CG_CC
Cuts at: 0 1528 2901
Size: 1528 1373
NcoI C'CATG_G
Cuts at: 0 1666 2901
Size: 1666 1235
NdeI CA'TA_TG
Cuts at: 0 2400 2901
Size: 2400 501
NspI r_CATG'y
Cuts at: 0 166 1908 2901
Size: 166 1742 993
Fragments arranged by size:
1742 993 166
PflMI CCAn_nnn'nTGG
Cuts at: 0 429 2901
Size: 429 2472
PfoI T'CCnGG_A
Cuts at: 0 585 836 1118 2901
Size: 585 251 282 1783
Fragments arranged by size:
1783 585 282 251
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 347 379 2901
Size: 347 32 2522
Fragments arranged by size:
2522 347 32
PpuMI rG'GwC_Cy
Cuts at: 0 47 2901
Size: 47 2854
PstI C_TGCA'G
Cuts at: 0 104 2901
Size: 104 2797
PvuII CAG'CTG
Cuts at: 0 99 2179 2901
Size: 99 2080 722
Fragments arranged by size:
2080 722 99
SacI G_AGCT'C
Cuts at: 0 1973 2246 2661 2901
Size: 1973 273 415 240
Fragments arranged by size:
1973 415 273 240
SalI G'TCGA_C
Cuts at: 0 1283 2901
Size: 1283 1618
SapI GCTCTTCn'nnn_
Cuts at: 0 2789 2901
Size: 2789 112
ScaI AGT'ACT
Cuts at: 0 80 2901
Size: 80 2821
SfcI C'TryA_G
Cuts at: 0 100 1292 2102 2706 2750 2901
Size: 100 1192 810 604 44 151
Fragments arranged by size:
1192 810 604 151 100 44
SfoI GGC'GCC
Cuts at: 0 1529 2901
Size: 1529 1372
SmlI C'TyrA_G
Cuts at: 0 1781 2901
Size: 1781 1120
SphI G_CATG'C
Cuts at: 0 1908 2901
Size: 1908 993
SspI AAT'ATT
Cuts at: 0 2115 2901
Size: 2115 786
StuI AGG'CCT
Cuts at: 0 1201 2901
Size: 1201 1700
StyI C'CwwG_G
Cuts at: 0 43 1202 1666 1889 2901
Size: 43 1159 464 223 1012
Fragments arranged by size:
1159 1012 464 223 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 588 592 983 2035 2806 2901
Size: 588 4 391 1052 771 95
Fragments arranged by size:
1052 771 588 391 95 4
TatI w'GTAC_w
Cuts at: 0 78 1219 2901
Size: 78 1141 1682
Fragments arranged by size:
1682 1141 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 466 498 567 599 2901
Size: 466 32 69 32 2302
Fragments arranged by size:
2302 466 69 32 32
XbaI T'CTAG_A
Cuts at: 0 1416 1945 2901
Size: 1416 529 956
Fragments arranged by size:
1416 956 529
Enzymes that do cut:
AccI AcuI AlfI AloI AlwNI ApoI AvrII BamHI
BanI BanII BbeI BbsI BcgI BciVI BglI BglII
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BstEII BstXI BstYI
BstZ17I BtgI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII HindIII KasI MfeI
MmeI MscI MspA1I NarI NcoI NdeI NspI PflMI
PfoI PpiI PpuMI PstI PvuII SacI SalI SapI
ScaI SfcI SfoI SmlI SphI SspI StuI StyI
TaqII TatI TstI XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaI ApaLI AscI AseI AsiSI AvaI
BaeI BbvCI BclI BfrBI BlpI Bme1580I BmgBI BmtI
BsaAI BsaXI BsiEI BsiWI BsrBI BsrGI BssHII BssSI
BstAPI BstBI Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EagI EciI EcoNI EcoRV FseI FspI FspAI
HpaI KpnI MluI MslI NaeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI Tth111I XcmI XhoI XmaI XmnI ZraI