Restriction map for
pF1KSDA0763
(Linear) (Six-base) MAPSORT of: KIAA0763.seq Check: 9404 from: 1 to: 2523
KIAA0763.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AfeI AGC'GCT
Cuts at: 0 100 1534 2523
Size: 100 1434 989
Fragments arranged by size:
1434 989 100
AflIII A'CryG_T
Cuts at: 0 1628 2523
Size: 1628 895
AhdI GACnn_n'nnGTC
Cuts at: 0 606 1788 2523
Size: 606 1182 735
Fragments arranged by size:
1182 735 606
AleI CACnn'nnGTG
Cuts at: 0 1478 2523
Size: 1478 1045
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2425 2523
Size: 2425 98
AlwNI CAG_nnn'CTG
Cuts at: 0 747 2073 2523
Size: 747 1326 450
Fragments arranged by size:
1326 747 450
ApaI G_GGCC'C
Cuts at: 0 462 669 2523
Size: 462 207 1854
Fragments arranged by size:
1854 462 207
ApaLI G'TGCA_C
Cuts at: 0 193 2523
Size: 193 2330
ApoI r'AATT_y
Cuts at: 0 1462 2188 2479 2523
Size: 1462 726 291 44
Fragments arranged by size:
1462 726 291 44
AvaI C'yCGr_G
Cuts at: 0 461 926 1651 1852 2260 2523
Size: 461 465 725 201 408 263
Fragments arranged by size:
725 465 461 408 263 201
BamHI G'GATC_C
Cuts at: 0 1751 1840 2523
Size: 1751 89 683
Fragments arranged by size:
1751 683 89
BanI G'GyrC_C
Cuts at: 0 23 435 907 2523
Size: 23 412 472 1616
Fragments arranged by size:
1616 472 412 23
BanII G_rGCy'C
Cuts at: 0 258 293 462 669 1859 2261 2523
Size: 258 35 169 207 1190 402 262
Fragments arranged by size:
1190 402 262 258 207 169 35
BbeI G_GCGC'C
Cuts at: 0 27 911 2523
Size: 27 884 1612
Fragments arranged by size:
1612 884 27
BbvCI CC'TCA_GC
Cuts at: 0 283 1339 2398 2523
Size: 283 1056 1059 125
Fragments arranged by size:
1059 1056 283 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2347 2381 2523
Size: 2347 34 142
Fragments arranged by size:
2347 142 34
BglI GCCn_nnn'nGGC
Cuts at: 0 960 2523
Size: 960 1563
BglII A'GATC_T
Cuts at: 0 2000 2523
Size: 2000 523
BlpI GC'TnA_GC
Cuts at: 0 993 1132 2523
Size: 993 139 1391
Fragments arranged by size:
1391 993 139
Bme1580I G_kGCm'C
Cuts at: 0 197 462 669 1292 2523
Size: 197 265 207 623 1231
Fragments arranged by size:
1231 623 265 207 197
BmgBI CAC'GTC
Cuts at: 0 1061 1398 2523
Size: 1061 337 1125
Fragments arranged by size:
1125 1061 337
BmrI ACTGGGnnnn_n'
Cuts at: 0 672 2292 2523
Size: 672 1620 231
Fragments arranged by size:
1620 672 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 370 748 2523
Size: 303 67 378 1775
Fragments arranged by size:
1775 378 303 67
Bpu10I CC'TnA_GC
Cuts at: 0 283 1339 2398 2523
Size: 283 1056 1059 125
Fragments arranged by size:
1059 1056 283 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 820 2159 2368 2523
Size: 286 534 1339 209 155
Fragments arranged by size:
1339 534 286 209 155
BsaI GGTCTCn'nnnn_
Cuts at: 0 228 2523
Size: 228 2295
BsaBI GATnn'nnATC
Cuts at: 0 1738 2523
Size: 1738 785
BsaHI Gr'CG_yC
Cuts at: 0 24 335 356 908 2276 2442 2523
Size: 24 311 21 552 1368 166 81
Fragments arranged by size:
1368 552 311 166 81 24 21
BsaWI w'CCGG_w
Cuts at: 0 1571 2180 2523
Size: 1571 609 343
Fragments arranged by size:
1571 609 343
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 226 245 256 275 553 583 814 844
Size: 226 19 11 19 278 30 231 30
Cuts at: 844 925 955 1075 1105 2523
Size: 81 30 120 30 1418
Fragments arranged by size:
1418 278 231 226 120 81 30 30
30 30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 445 574 577 946 2523
Size: 445 129 3 369 1577
Fragments arranged by size:
1577 445 369 129 3
BseYI C'CCAG_C
Cuts at: 0 245 249 640 787 1169 2287 2523
Size: 245 4 391 147 382 1118 236
Fragments arranged by size:
1118 391 382 245 236 147 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 472 1819 2523
Size: 406 66 1347 704
Fragments arranged by size:
1347 704 406 66
BsiEI CG_ry'CG
Cuts at: 0 607 1838 2523
Size: 607 1231 685
Fragments arranged by size:
1231 685 607
BsiHKAI G_wGCw'C
Cuts at: 0 197 718 1403 1871 2261 2305 2521 2523
Size: 197 521 685 468 390 44 216 2
Fragments arranged by size:
685 521 468 390 216 197 44 2
Bsp1286I G_dGCh'C
Cuts at: 0 197 258 293 462 669 718 1292 1403
Size: 197 61 35 169 207 49 574 111
Cuts at: 1403 1859 1871 2261 2305 2521 2523
Size: 456 12 390 44 216 2
Fragments arranged by size:
574 456 390 216 207 197 169 111
61 49 44 35 12 2
BspEI T'CCGG_A
Cuts at: 0 1571 2523
Size: 1571 952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 210 635 2058 2210 2523
Size: 210 425 1423 152 313
Fragments arranged by size:
1423 425 313 210 152
BsrBI CCG'CTC
Cuts at: 0 732 818 825 1509 1656 2523
Size: 732 86 7 684 147 867
Fragments arranged by size:
867 732 684 147 86 7
BsrDI GCAATG_nn'
Cuts at: 0 2213 2478 2523
Size: 2213 265 45
Fragments arranged by size:
2213 265 45
BsrFI r'CCGG_y
Cuts at: 0 438 602 1001 1343 1373 1904 2523
Size: 438 164 399 342 30 531 619
Fragments arranged by size:
619 531 438 399 342 164 30
BsrGI T'GTAC_A
Cuts at: 0 548 1631 2523
Size: 548 1083 892
Fragments arranged by size:
1083 892 548
BssSI C'ACGA_G
Cuts at: 0 702 2523
Size: 702 1821
BstAPI GCAn_nnn'nTGC
Cuts at: 0 747 2073 2523
Size: 747 1326 450
Fragments arranged by size:
1326 747 450
BstEII G'GTnAC_C
Cuts at: 0 30 277 1992 2523
Size: 30 247 1715 531
Fragments arranged by size:
1715 531 247 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 662 966 1443 2523
Size: 662 304 477 1080
Fragments arranged by size:
1080 662 477 304
BstYI r'GATC_y
Cuts at: 0 1739 1751 1840 2000 2523
Size: 1739 12 89 160 523
Fragments arranged by size:
1739 523 160 89 12
Bsu36I CC'TnA_GG
Cuts at: 0 1456 2523
Size: 1456 1067
BtgI C'CryG_G
Cuts at: 0 1436 2523
Size: 1436 1087
BtsI GCAGTG_nn'
Cuts at: 0 2457 2497 2523
Size: 2457 40 26
Fragments arranged by size:
2457 40 26
ClaI AT'CG_AT
Cuts at: 0 554 2523
Size: 554 1969
DrdI GACnn_nn'nnGTC
Cuts at: 0 1300 2523
Size: 1300 1223
EaeI y'GGCC_r
Cuts at: 0 383 604 980 2523
Size: 383 221 376 1543
Fragments arranged by size:
1543 383 376 221
EagI C'GGCC_G
Cuts at: 0 604 2523
Size: 604 1919
EarI CTCTTCn'nnn_
Cuts at: 0 1240 2083 2523
Size: 1240 843 440
Fragments arranged by size:
1240 843 440
EcoICRI GAG'CTC
Cuts at: 0 2259 2523
Size: 2259 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 370 748 2523
Size: 303 67 378 1775
Fragments arranged by size:
1775 378 303 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 363 589 2314 2523
Size: 363 226 1725 209
Fragments arranged by size:
1725 363 226 209
EcoO109I rG'GnC_Cy
Cuts at: 0 181 666 962 1335 1452 2070 2379 2415
Size: 181 485 296 373 117 618 309 36
Cuts at: 2415 2523
Size: 108
Fragments arranged by size:
618 485 373 309 296 181 117 108
36
EcoRV GAT'ATC
Cuts at: 0 2139 2523
Size: 2139 384
FspI TGC'GCA
Cuts at: 0 106 2523
Size: 106 2417
HaeII r_GCGC'y
Cuts at: 0 27 102 911 1536 2396 2523
Size: 27 75 809 625 860 127
Fragments arranged by size:
860 809 625 127 75 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 553 585 592 624 814 846
Size: 346 32 175 32 7 32 190 32
Cuts at: 846 1087 1119 1774 1806 2123 2155 2523
Size: 241 32 655 32 317 32 368
Fragments arranged by size:
655 368 346 317 241 190 175 32
32 32 32 32 32 32 7
KasI G'GCGC_C
Cuts at: 0 23 907 2523
Size: 23 884 1616
Fragments arranged by size:
1616 884 23
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 602 1001 1088 1094 2064 2234 2523
Size: 176 426 399 87 6 970 170 289
Fragments arranged by size:
970 426 399 289 176 170 87 6
MscI TGG'CCA
Cuts at: 0 982 2523
Size: 982 1541
MslI CAynn'nnrTG
Cuts at: 0 160 1478 1627 2523
Size: 160 1318 149 896
Fragments arranged by size:
1318 896 160 149
MspA1I CmG'CkG
Cuts at: 0 249 744 768 905 1169 2372 2523
Size: 249 495 24 137 264 1203 151
Fragments arranged by size:
1203 495 264 249 151 137 24
NaeI GCC'GGC
Cuts at: 0 1345 1906 2523
Size: 1345 561 617
Fragments arranged by size:
1345 617 561
NarI GG'CG_CC
Cuts at: 0 24 908 2523
Size: 24 884 1615
Fragments arranged by size:
1615 884 24
NcoI C'CATG_G
Cuts at: 0 1436 2523
Size: 1436 1087
NgoMIV G'CCGG_C
Cuts at: 0 1343 1904 2523
Size: 1343 561 619
Fragments arranged by size:
1343 619 561
NspI r_CATG'y
Cuts at: 0 132 1632 2067 2295 2523
Size: 132 1500 435 228 228
Fragments arranged by size:
1500 435 228 228 132
PasI CC'CwG_GG
Cuts at: 0 1552 2523
Size: 1552 971
PciI A'CATG_T
Cuts at: 0 1628 2523
Size: 1628 895
PflMI CCAn_nnn'nTGG
Cuts at: 0 2232 2523
Size: 2232 291
PpuMI rG'GwC_Cy
Cuts at: 0 2070 2415 2523
Size: 2070 345 108
Fragments arranged by size:
2070 345 108
PspOMI G'GGCC_C
Cuts at: 0 458 665 2523
Size: 458 207 1858
Fragments arranged by size:
1858 458 207
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2077 2109 2523
Size: 2077 32 414
Fragments arranged by size:
2077 414 32
PstI C_TGCA'G
Cuts at: 0 1090 1328 2504 2523
Size: 1090 238 1176 19
Fragments arranged by size:
1176 1090 238 19
PvuI CG_AT'CG
Cuts at: 0 1838 2523
Size: 1838 685
PvuII CAG'CTG
Cuts at: 0 249 1169 2523
Size: 249 920 1354
Fragments arranged by size:
1354 920 249
SacI G_AGCT'C
Cuts at: 0 2261 2523
Size: 2261 262
SapI GCTCTTCn'nnn_
Cuts at: 0 2083 2523
Size: 2083 440
ScaI AGT'ACT
Cuts at: 0 2092 2523
Size: 2092 431
SfcI C'TryA_G
Cuts at: 0 1086 1324 2500 2523
Size: 1086 238 1176 23
Fragments arranged by size:
1176 1086 238 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 960 2523
Size: 960 1563
SfoI GGC'GCC
Cuts at: 0 25 909 2523
Size: 25 884 1614
Fragments arranged by size:
1614 884 25
SmaI CCC'GGG
Cuts at: 0 463 928 1854 2523
Size: 463 465 926 669
Fragments arranged by size:
926 669 465 463
SmlI C'TyrA_G
Cuts at: 0 301 835 2174 2260 2383 2523
Size: 301 534 1339 86 123 140
Fragments arranged by size:
1339 534 301 140 123 86
SphI G_CATG'C
Cuts at: 0 2067 2523
Size: 2067 456
StyI C'CwwG_G
Cuts at: 0 864 1436 1485 2523
Size: 864 572 49 1038
Fragments arranged by size:
1038 864 572 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 801 2523
Size: 801 1722
TatI w'GTAC_w
Cuts at: 0 548 1631 2090 2523
Size: 548 1083 459 433
Fragments arranged by size:
1083 548 459 433
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 245 277 2523
Size: 245 32 2246
Fragments arranged by size:
2246 245 32
Tth111I GACn'n_nGTC
Cuts at: 0 1408 2523
Size: 1408 1115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 1593 2523
Size: 345 1248 930
Fragments arranged by size:
1248 930 345
XhoI C'TCGA_G
Cuts at: 0 2260 2523
Size: 2260 263
XmaI C'CCGG_G
Cuts at: 0 461 926 1852 2523
Size: 461 465 926 671
Fragments arranged by size:
926 671 465 461
XmnI GAAnn'nnTTC
Cuts at: 0 1234 1384 2188 2523
Size: 1234 150 804 335
Fragments arranged by size:
1234 804 335 150
Enzymes that do cut:
AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI ClaI DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PpuMI PspOMI PsrI PstI
PvuI PvuII SacI SapI ScaI SfcI SfiI SfoI
SmaI SmlI SphI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AloI AscI AseI AsiSI AvrII BaeI BbsI BciVI
BclI BfrBI BmtI BplI BsaAI BsiWI BsmI BsmBI
BspHI BssHII BstBI BstZ17I BtgZI CspCI DraI DraIII
EciI EcoRI FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
RsrII SacII SalI SanDI SbfI SexAI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI XbaI ZraI