Restriction map for
pF1KSDA0764
(Linear) (Six-base) MAPSORT of: KIAA0764.seq Check: 9726 from: 1 to: 1242
KIAA0764.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AatII G_ACGT'C
Cuts at: 0 225 1242
Size: 225 1017
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 782 1242
Size: 308 474 460
Fragments arranged by size:
474 460 308
AfeI AGC'GCT
Cuts at: 0 1216 1242
Size: 1216 26
AflII C'TTAA_G
Cuts at: 0 571 1242
Size: 571 671
AlwNI CAG_nnn'CTG
Cuts at: 0 903 1242
Size: 903 339
BanII G_rGCy'C
Cuts at: 0 927 1141 1242
Size: 927 214 101
Fragments arranged by size:
927 214 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 217 1083 1242
Size: 217 866 159
Fragments arranged by size:
866 217 159
BbvCI CC'TCA_GC
Cuts at: 0 126 1242
Size: 126 1116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 442 476 1242
Size: 442 34 766
Fragments arranged by size:
766 442 34
Bme1580I G_kGCm'C
Cuts at: 0 316 1242
Size: 316 926
BmrI ACTGGGnnnn_n'
Cuts at: 0 27 1242
Size: 27 1215
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 317 1242
Size: 317 925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 676 892 911 973 1242
Size: 556 120 216 19 62 269
Fragments arranged by size:
556 269 216 120 62 19
Bpu10I CC'TnA_GC
Cuts at: 0 126 248 1242
Size: 126 122 994
Fragments arranged by size:
994 126 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1242
Size: 1022 220
BsaI GGTCTCn'nnnn_
Cuts at: 0 532 887 1242
Size: 532 355 355
Fragments arranged by size:
532 355 355
BsaHI Gr'CG_yC
Cuts at: 0 222 1242
Size: 222 1020
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 314 344 1090 1120 1242
Size: 314 30 746 30 122
Fragments arranged by size:
746 314 122 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 317 460 880 992 1111 1242
Size: 317 143 420 112 119 131
Fragments arranged by size:
420 317 143 131 119 112
BseYI C'CCAG_C
Cuts at: 0 618 1077 1123 1242
Size: 618 459 46 119
Fragments arranged by size:
618 459 119 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 1004 1242
Size: 100 904 238
Fragments arranged by size:
904 238 100
BsiHKAI G_wGCw'C
Cuts at: 0 686 1242
Size: 686 556
Bsp1286I G_dGCh'C
Cuts at: 0 316 686 927 1141 1242
Size: 316 370 241 214 101
Fragments arranged by size:
370 316 241 214 101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 642 1242
Size: 642 600
BsrDI GCAATG_nn'
Cuts at: 0 1062 1242
Size: 1062 180
BssSI C'ACGA_G
Cuts at: 0 309 1242
Size: 309 933
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1074 1242
Size: 1074 168
BstEII G'GTnAC_C
Cuts at: 0 320 1242
Size: 320 922
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 495 1242
Size: 156 339 747
Fragments arranged by size:
747 339 156
BstYI r'GATC_y
Cuts at: 0 383 1242
Size: 383 859
BtgI C'CryG_G
Cuts at: 0 76 1242
Size: 76 1166
BtsI GCAGTG_nn'
Cuts at: 0 488 684 901 1089 1242
Size: 488 196 217 188 153
Fragments arranged by size:
488 217 196 188 153
EaeI y'GGCC_r
Cuts at: 0 485 1242
Size: 485 757
EarI CTCTTCn'nnn_
Cuts at: 0 284 308 1219 1242
Size: 284 24 911 23
Fragments arranged by size:
911 284 24 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 556 676 782 892 911 973 1242
Size: 308 248 120 106 110 19 62 269
Fragments arranged by size:
308 269 248 120 110 106 62 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 475 1106 1242
Size: 475 631 136
Fragments arranged by size:
631 475 136
EcoO109I rG'GnC_Cy
Cuts at: 0 1193 1242
Size: 1193 49
HaeII r_GCGC'y
Cuts at: 0 1218 1242
Size: 1218 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 768 800 1090 1122 1242
Size: 151 32 585 32 290 32 120
Fragments arranged by size:
585 290 151 120 32 32 32
HindIII A'AGCT_T
Cuts at: 0 962 1242
Size: 962 280
MscI TGG'CCA
Cuts at: 0 487 1242
Size: 487 755
MspA1I CmG'CkG
Cuts at: 0 449 1242
Size: 449 793
NspI r_CATG'y
Cuts at: 0 738 1242
Size: 738 504
PasI CC'CwG_GG
Cuts at: 0 316 1242
Size: 316 926
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 666 1242
Size: 483 183 576
Fragments arranged by size:
576 483 183
PfoI T'CCnGG_A
Cuts at: 0 533 1242
Size: 533 709
PpuMI rG'GwC_Cy
Cuts at: 0 1193 1242
Size: 1193 49
PvuII CAG'CTG
Cuts at: 0 449 1242
Size: 449 793
SfcI C'TryA_G
Cuts at: 0 739 1242
Size: 739 503
SmlI C'TyrA_G
Cuts at: 0 571 1037 1242
Size: 571 466 205
Fragments arranged by size:
571 466 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 68 100 314 346 1242
Size: 68 32 214 32 896
Fragments arranged by size:
896 214 68 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 302 1242
Size: 302 940
ZraI GAC'GTC
Cuts at: 0 223 1242
Size: 223 1019
Enzymes that do cut:
AatII AcuI AfeI AflII AlwNI BanII BbsI BbvCI
BcgI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI
EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII
MscI MspA1I NspI PasI PflMI PfoI PpuMI PvuII
SfcI SmlI TstI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MmeI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmaI