Restriction map for
pF1KA0765
(Linear) (Six-base) MAPSORT of: KIAA0765.seq Check: 9215 from: 1 to: 2796
KIAA0765.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AclI AA'CG_TT
Cuts at: 0 508 2796
Size: 508 2288
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1554 1666 2249 2796
Size: 1554 112 583 547
Fragments arranged by size:
1554 583 547 112
AflIII A'CryG_T
Cuts at: 0 1332 1722 2796
Size: 1332 390 1074
Fragments arranged by size:
1332 1074 390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2044 2796
Size: 2044 752
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 459 491 2288 2320 2796
Size: 459 32 1797 32 476
Fragments arranged by size:
1797 476 459 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2737 2796
Size: 2737 59
ApaI G_GGCC'C
Cuts at: 0 2365 2796
Size: 2365 431
ApoI r'AATT_y
Cuts at: 0 798 2370 2796
Size: 798 1572 426
Fragments arranged by size:
1572 798 426
AvaI C'yCGr_G
Cuts at: 0 2406 2717 2796
Size: 2406 311 79
Fragments arranged by size:
2406 311 79
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 350 383 2796
Size: 350 33 2413
Fragments arranged by size:
2413 350 33
BanI G'GyrC_C
Cuts at: 0 756 2796
Size: 756 2040
BanII G_rGCy'C
Cuts at: 0 505 980 2365 2796
Size: 505 475 1385 431
Fragments arranged by size:
1385 505 475 431
BciVI GTATCCnnnnn_n'
Cuts at: 0 378 2796
Size: 378 2418
BglI GCCn_nnn'nGGC
Cuts at: 0 1245 2704 2796
Size: 1245 1459 92
Fragments arranged by size:
1459 1245 92
Bme1580I G_kGCm'C
Cuts at: 0 671 1638 2365 2445 2796
Size: 671 967 727 80 351
Fragments arranged by size:
967 727 671 351 80
BmrI ACTGGGnnnn_n'
Cuts at: 0 127 386 676 1235 1696 1982 2072 2796
Size: 127 259 290 559 461 286 90 724
Fragments arranged by size:
724 559 461 290 286 259 127 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 806 1175 2201 2796
Size: 620 186 369 1026 595
Fragments arranged by size:
1026 620 595 369 186
Bpu10I CC'TnA_GC
Cuts at: 0 330 2394 2796
Size: 330 2064 402
Fragments arranged by size:
2064 402 330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1043 2796
Size: 1043 1753
BsaI GGTCTCn'nnnn_
Cuts at: 0 31 2796
Size: 31 2765
BsaAI yAC'GTr
Cuts at: 0 1795 2796
Size: 1795 1001
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 459 489 522 552 636 666 2796
Size: 459 30 33 30 84 30 2130
Fragments arranged by size:
2130 459 84 33 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 464 623 1202 2204 2796
Size: 464 159 579 1002 592
Fragments arranged by size:
1002 592 579 464 159
BseYI C'CCAG_C
Cuts at: 0 2017 2796
Size: 2017 779
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2012 2027 2072 2102 2117 2796
Size: 2012 15 45 30 15 679
Fragments arranged by size:
2012 679 45 30 15 15
BsiHKAI G_wGCw'C
Cuts at: 0 792 2796
Size: 792 2004
BsmI GAATG_Cn'
Cuts at: 0 935 1097 1958 2078 2796
Size: 935 162 861 120 718
Fragments arranged by size:
935 861 718 162 120
Bsp1286I G_dGCh'C
Cuts at: 0 505 671 792 980 1638 2365 2445 2796
Size: 505 166 121 188 658 727 80 351
Fragments arranged by size:
727 658 505 351 188 166 121 80
BspHI T'CATG_A
Cuts at: 0 420 2796
Size: 420 2376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 774 2796
Size: 774 2022
BsrDI GCAATG_nn'
Cuts at: 0 646 950 2796
Size: 646 304 1846
Fragments arranged by size:
1846 646 304
BsrGI T'GTAC_A
Cuts at: 0 1719 2796
Size: 1719 1077
BstAPI GCAn_nnn'nTGC
Cuts at: 0 930 2586 2796
Size: 930 1656 210
Fragments arranged by size:
1656 930 210
BstBI TT'CG_AA
Cuts at: 0 1551 2796
Size: 1551 1245
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2521 2796
Size: 2521 275
BtgI C'CryG_G
Cuts at: 0 47 2699 2796
Size: 47 2652 97
Fragments arranged by size:
2652 97 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 598 2796
Size: 598 2198
BtsI GCAGTG_nn'
Cuts at: 0 709 882 998 2796
Size: 709 173 116 1798
Fragments arranged by size:
1798 709 173 116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2319 2354 2796
Size: 2319 35 442
Fragments arranged by size:
2319 442 35
DraI TTT'AAA
Cuts at: 0 1350 1776 2663 2796
Size: 1350 426 887 133
Fragments arranged by size:
1350 887 426 133
DraIII CAC_nnn'GTG
Cuts at: 0 394 2796
Size: 394 2402
EarI CTCTTCn'nnn_
Cuts at: 0 2102 2796
Size: 2102 694
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 734 2796
Size: 734 2062
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 806 1175 1554 1666 2201 2249 2796
Size: 620 186 369 379 112 535 48 547
Fragments arranged by size:
620 547 535 379 369 186 112 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 329 1898 1928 2096 2216 2291 2796
Size: 329 1569 30 168 120 75 505
Fragments arranged by size:
1569 505 329 168 120 75 30
EcoO109I rG'GnC_Cy
Cuts at: 0 1186 2234 2361 2362 2796
Size: 1186 1048 127 1 434
Fragments arranged by size:
1186 1048 434 127 1
FspI TGC'GCA
Cuts at: 0 175 2796
Size: 175 2621
FspAI rTGC'GCAy
Cuts at: 0 175 2796
Size: 175 2621
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 576 608 852 884 1148 1180 1584 1603
Size: 576 32 244 32 264 32 404 19
Cuts at: 1603 1616 1635 2796
Size: 13 19 1161
Fragments arranged by size:
1161 576 404 264 244 32 32 32
19 19 13
HincII GTy'rAC
Cuts at: 0 849 1617 2796
Size: 849 768 1179
Fragments arranged by size:
1179 849 768
HindIII A'AGCT_T
Cuts at: 0 1073 1838 2796
Size: 1073 765 958
Fragments arranged by size:
1073 958 765
HpaI GTT'AAC
Cuts at: 0 849 2796
Size: 849 1947
MfeI C'AATT_G
Cuts at: 0 32 2796
Size: 32 2764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 2250 2796
Size: 395 1855 546
Fragments arranged by size:
1855 546 395
MslI CAynn'nnrTG
Cuts at: 0 2456 2519 2796
Size: 2456 63 277
Fragments arranged by size:
2456 277 63
MspA1I CmG'CkG
Cuts at: 0 2734 2796
Size: 2734 62
NcoI C'CATG_G
Cuts at: 0 47 2699 2796
Size: 47 2652 97
Fragments arranged by size:
2652 97 47
NspI r_CATG'y
Cuts at: 0 732 1336 1726 2116 2589 2796
Size: 732 604 390 390 473 207
Fragments arranged by size:
732 604 473 390 390 207
PasI CC'CwG_GG
Cuts at: 0 2446 2796
Size: 2446 350
PciI A'CATG_T
Cuts at: 0 1332 1722 2796
Size: 1332 390 1074
Fragments arranged by size:
1332 1074 390
PflMI CCAn_nnn'nTGG
Cuts at: 0 2473 2796
Size: 2473 323
PfoI T'CCnGG_A
Cuts at: 0 1947 2217 2796
Size: 1947 270 579
Fragments arranged by size:
1947 579 270
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 459 491 2796
Size: 459 32 2305
Fragments arranged by size:
2305 459 32
PpuMI rG'GwC_Cy
Cuts at: 0 1186 2796
Size: 1186 1610
PspOMI G'GGCC_C
Cuts at: 0 2361 2796
Size: 2361 435
PstI C_TGCA'G
Cuts at: 0 1565 1944 2796
Size: 1565 379 852
Fragments arranged by size:
1565 852 379
PvuII CAG'CTG
Cuts at: 0 2734 2796
Size: 2734 62
SapI GCTCTTCn'nnn_
Cuts at: 0 2102 2796
Size: 2102 694
SfcI C'TryA_G
Cuts at: 0 1561 1940 2126 2762 2796
Size: 1561 379 186 636 34
Fragments arranged by size:
1561 636 379 186 34
SmaI CCC'GGG
Cuts at: 0 2408 2796
Size: 2408 388
SmlI C'TyrA_G
Cuts at: 0 1058 2796
Size: 1058 1738
SphI G_CATG'C
Cuts at: 0 732 2116 2796
Size: 732 1384 680
Fragments arranged by size:
1384 732 680
SspI AAT'ATT
Cuts at: 0 1650 2796
Size: 1650 1146
StuI AGG'CCT
Cuts at: 0 2012 2253 2760 2796
Size: 2012 241 507 36
Fragments arranged by size:
2012 507 241 36
StyI C'CwwG_G
Cuts at: 0 47 1899 2699 2796
Size: 47 1852 800 97
Fragments arranged by size:
1852 800 97 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 109 405 714 732 2446 2577 2796
Size: 109 296 309 18 1714 131 219
Fragments arranged by size:
1714 309 296 219 131 109 18
TatI w'GTAC_w
Cuts at: 0 1478 1719 2796
Size: 1478 241 1077
Fragments arranged by size:
1478 1077 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 522 554 1688 1720 2796
Size: 522 32 1134 32 1076
Fragments arranged by size:
1134 1076 522 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 67 2796
Size: 67 2729
XmaI C'CCGG_G
Cuts at: 0 2406 2796
Size: 2406 390
Enzymes that do cut:
AclI AcuI AflIII AlfI AloI AlwNI ApaI ApoI
AvaI BaeI BanI BanII BciVI BglI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI
BstAPI BstBI BstXI BtgI BtgZI BtsI CspCI DraI
DraIII EarI EciI Eco57MI EcoNI EcoO109I FspI FspAI
Hin4I HincII HindIII HpaI MfeI MmeI MslI MspA1I
NcoI NspI PasI PciI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII SapI SfcI SmaI SmlI SphI
SspI StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI ApaLI AscI AseI AsiSI AvrII BamHI BbeI
BbsI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstEII BstYI BstZ17I
Bsu36I ClaI DrdI EaeI EagI EcoICRI EcoRI EcoRV
FalI FseI HaeII KasI KpnI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI Tth111I
XbaI XhoI XmnI ZraI