Restriction map for
pF1KSDA0769
(Linear) (Six-base) MAPSORT of: KIAA0769.seq Check: 4871 from: 1 to: 2052
KIAA0769.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1258 2052
Size: 1258 794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 570 664 1016 1862 2052
Size: 46 524 94 352 846 190
Fragments arranged by size:
846 524 352 190 94 46
AflII C'TTAA_G
Cuts at: 0 1230 2052
Size: 1230 822
AflIII A'CryG_T
Cuts at: 0 829 831 1475 2052
Size: 829 2 644 577
Fragments arranged by size:
829 644 577 2
AhdI GACnn_n'nnGTC
Cuts at: 0 1467 2052
Size: 1467 585
AleI CACnn'nnGTG
Cuts at: 0 832 2047 2052
Size: 832 1215 5
Fragments arranged by size:
1215 832 5
AlwNI CAG_nnn'CTG
Cuts at: 0 778 1449 2052
Size: 778 671 603
Fragments arranged by size:
778 671 603
ApaI G_GGCC'C
Cuts at: 0 1101 2052
Size: 1101 951
ApoI r'AATT_y
Cuts at: 0 1663 2052
Size: 1663 389
AvaI C'yCGr_G
Cuts at: 0 103 2052
Size: 103 1949
BanI G'GyrC_C
Cuts at: 0 1225 2052
Size: 1225 827
BanII G_rGCy'C
Cuts at: 0 1101 1855 2052
Size: 1101 754 197
Fragments arranged by size:
1101 754 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 1352 2052
Size: 1352 700
BciVI GTATCCnnnnn_n'
Cuts at: 0 92 2052
Size: 92 1960
BclI T'GATC_A
Cuts at: 0 558 2052
Size: 558 1494
BglI GCCn_nnn'nGGC
Cuts at: 0 1223 2052
Size: 1223 829
BglII A'GATC_T
Cuts at: 0 1757 2052
Size: 1757 295
Bme1580I G_kGCm'C
Cuts at: 0 1101 2052
Size: 1101 951
BmgBI CAC'GTC
Cuts at: 0 1478 2052
Size: 1478 574
BmrI ACTGGGnnnn_n'
Cuts at: 0 120 788 1359 2052
Size: 120 668 571 693
Fragments arranged by size:
693 668 571 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 636 907 2052
Size: 62 574 271 1145
Fragments arranged by size:
1145 574 271 62
Bpu10I CC'TnA_GC
Cuts at: 0 1432 2052
Size: 1432 620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 2052
Size: 550 1502
BsaI GGTCTCn'nnnn_
Cuts at: 0 1877 1939 2052
Size: 1877 62 113
Fragments arranged by size:
1877 113 62
BsaAI yAC'GTr
Cuts at: 0 832 841 2052
Size: 832 9 1211
Fragments arranged by size:
1211 832 9
BsaBI GATnn'nnATC
Cuts at: 0 1756 2052
Size: 1756 296
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 890 920 2052
Size: 890 30 1132
Fragments arranged by size:
1132 890 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 805 1863 2052
Size: 805 1058 189
Fragments arranged by size:
1058 805 189
BseYI C'CCAG_C
Cuts at: 0 719 1819 1834 2052
Size: 719 1100 15 218
Fragments arranged by size:
1100 719 218 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 2052
Size: 640 1412
BsiHKAI G_wGCw'C
Cuts at: 0 797 1855 2052
Size: 797 1058 197
Fragments arranged by size:
1058 797 197
Bsp1286I G_dGCh'C
Cuts at: 0 797 1101 1855 2052
Size: 797 304 754 197
Fragments arranged by size:
797 754 304 197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 108 1019 1258 2052
Size: 108 911 239 794
Fragments arranged by size:
911 794 239 108
BstAPI GCAn_nnn'nTGC
Cuts at: 0 487 2052
Size: 487 1565
BstXI CCAn_nnnn'nTGG
Cuts at: 0 660 834 2052
Size: 660 174 1218
Fragments arranged by size:
1218 660 174
BstYI r'GATC_y
Cuts at: 0 239 1757 2052
Size: 239 1518 295
Fragments arranged by size:
1518 295 239
Bsu36I CC'TnA_GG
Cuts at: 0 1598 2052
Size: 1598 454
BtgI C'CryG_G
Cuts at: 0 1490 1738 2052
Size: 1490 248 314
Fragments arranged by size:
1490 314 248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1107 2052
Size: 1107 945
BtsI GCAGTG_nn'
Cuts at: 0 1118 1795 2052
Size: 1118 677 257
Fragments arranged by size:
1118 677 257
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1784 1819 2052
Size: 1784 35 233
Fragments arranged by size:
1784 233 35
EaeI y'GGCC_r
Cuts at: 0 1374 1451 2052
Size: 1374 77 601
Fragments arranged by size:
1374 601 77
EarI CTCTTCn'nnn_
Cuts at: 0 22 1166 2052
Size: 22 1144 886
Fragments arranged by size:
1144 886 22
EcoICRI GAG'CTC
Cuts at: 0 1853 2052
Size: 1853 199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 62 570 636 664 907 1016 1862
Size: 46 16 508 66 28 243 109 846
Cuts at: 1862 2052
Size: 190
Fragments arranged by size:
846 508 243 190 109 66 46 28
16
EcoRI G'AATT_C
Cuts at: 0 1663 2052
Size: 1663 389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 312 2052
Size: 312 1740
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 66 98 337 369 1453 1485 1573 1595
Size: 66 32 239 32 1084 32 88 22
Cuts at: 1595 1605 1627 2052
Size: 10 22 425
Fragments arranged by size:
1084 425 239 88 66 32 32 32
22 22 10
HincII GTy'rAC
Cuts at: 0 233 525 1295 2052
Size: 233 292 770 757
Fragments arranged by size:
770 757 292 233
HindIII A'AGCT_T
Cuts at: 0 192 2052
Size: 192 1860
HpaI GTT'AAC
Cuts at: 0 525 2052
Size: 525 1527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 986 2052
Size: 986 1066
MscI TGG'CCA
Cuts at: 0 1376 2052
Size: 1376 676
MslI CAynn'nnrTG
Cuts at: 0 832 2047 2052
Size: 832 1215 5
Fragments arranged by size:
1215 832 5
MspA1I CmG'CkG
Cuts at: 0 684 2052
Size: 684 1368
NcoI C'CATG_G
Cuts at: 0 1738 2052
Size: 1738 314
NdeI CA'TA_TG
Cuts at: 0 1953 2052
Size: 1953 99
NspI r_CATG'y
Cuts at: 0 81 611 1440 2052
Size: 81 530 829 612
Fragments arranged by size:
829 612 530 81
PflMI CCAn_nnn'nTGG
Cuts at: 0 1071 2052
Size: 1071 981
PfoI T'CCnGG_A
Cuts at: 0 665 2052
Size: 665 1387
PmlI CAC'GTG
Cuts at: 0 832 841 2052
Size: 832 9 1211
Fragments arranged by size:
1211 832 9
PspOMI G'GGCC_C
Cuts at: 0 1097 2052
Size: 1097 955
PstI C_TGCA'G
Cuts at: 0 1113 1445 2052
Size: 1113 332 607
Fragments arranged by size:
1113 607 332
PvuII CAG'CTG
Cuts at: 0 684 2052
Size: 684 1368
SacI G_AGCT'C
Cuts at: 0 1855 2052
Size: 1855 197
SfcI C'TryA_G
Cuts at: 0 1109 1405 1441 2052
Size: 1109 296 36 611
Fragments arranged by size:
1109 611 296 36
SmlI C'TyrA_G
Cuts at: 0 103 565 1230 2052
Size: 103 462 665 822
Fragments arranged by size:
822 665 462 103
SphI G_CATG'C
Cuts at: 0 1440 2052
Size: 1440 612
SspI AAT'ATT
Cuts at: 0 963 2052
Size: 963 1089
StyI C'CwwG_G
Cuts at: 0 1738 2052
Size: 1738 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1457 2052
Size: 1457 595
TatI w'GTAC_w
Cuts at: 0 1859 2052
Size: 1859 193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1642 2052
Size: 1642 410
XhoI C'TCGA_G
Cuts at: 0 103 2052
Size: 103 1949
XmnI GAAnn'nnTTC
Cuts at: 0 628 962 2052
Size: 628 334 1090
Fragments arranged by size:
1090 628 334
Enzymes that do cut:
AarI AcuI AflII AflIII AhdI AleI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BciVI BclI BglI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI EaeI EarI EcoICRI Eco57MI EcoRI FalI Hin4I
HincII HindIII HpaI MmeI MscI MslI MspA1I NcoI
NdeI NspI PflMI PfoI PmlI PspOMI PstI PvuII
SacI SfcI SmlI SphI SspI StyI TaqII TatI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BfrBI BlpI BmtI BplI BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI
DraI DraIII DrdI EagI EciI EcoNI EcoO109I EcoRV
FseI FspI FspAI HaeII KasI KpnI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TstI Tth111I XbaI XmaI
ZraI