Restriction map for
pF1KA0772
(Linear) (Six-base) MAPSORT of: KIAA0772.seq Check: 2870 from: 1 to: 1404
KIAA0772.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 679 1404
Size: 679 725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 223 1404
Size: 168 55 1181
Fragments arranged by size:
1181 168 55
AflIII A'CryG_T
Cuts at: 0 302 1404
Size: 302 1102
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1063 1404
Size: 1063 341
AlwNI CAG_nnn'CTG
Cuts at: 0 342 1404
Size: 342 1062
ApoI r'AATT_y
Cuts at: 0 16 131 1377 1404
Size: 16 115 1246 27
Fragments arranged by size:
1246 115 27 16
AseI AT'TA_AT
Cuts at: 0 448 1404
Size: 448 956
AvaI C'yCGr_G
Cuts at: 0 608 1404
Size: 608 796
BanI G'GyrC_C
Cuts at: 0 613 1404
Size: 613 791
BanII G_rGCy'C
Cuts at: 0 608 1404
Size: 608 796
BbsI GAAGACnn'nnnn_
Cuts at: 0 971 1153 1404
Size: 971 182 251
Fragments arranged by size:
971 251 182
BmgBI CAC'GTC
Cuts at: 0 990 1002 1404
Size: 990 12 402
Fragments arranged by size:
990 402 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 113 387 1343 1404
Size: 113 274 956 61
Fragments arranged by size:
956 274 113 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 132 134 1404
Size: 132 2 1270
Fragments arranged by size:
1270 132 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 364 646 1074 1150 1261 1404
Size: 241 123 282 428 76 111 143
Fragments arranged by size:
428 282 241 143 123 111 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 559 1404
Size: 104 455 845
Fragments arranged by size:
845 455 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 535 1012 1404
Size: 535 477 392
Fragments arranged by size:
535 477 392
BsaAI yAC'GTr
Cuts at: 0 303 1404
Size: 303 1101
BsaBI GATnn'nnATC
Cuts at: 0 244 477 1404
Size: 244 233 927
Fragments arranged by size:
927 244 233
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 289 307 319 337 1276 1306 1404
Size: 289 18 12 18 939 30 98
Fragments arranged by size:
939 289 98 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 942 1258 1297 1404
Size: 942 316 39 107
Fragments arranged by size:
942 316 107 39
BseYI C'CCAG_C
Cuts at: 0 335 1404
Size: 335 1069
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1404
Size: 1045 359
BsiHKAI G_wGCw'C
Cuts at: 0 302 1404
Size: 302 1102
Bsp1286I G_dGCh'C
Cuts at: 0 302 608 1404
Size: 302 306 796
Fragments arranged by size:
796 306 302
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 679 1017 1404
Size: 679 338 387
Fragments arranged by size:
679 387 338
BsrBI CCG'CTC
Cuts at: 0 1236 1272 1374 1404
Size: 1236 36 102 30
Fragments arranged by size:
1236 102 36 30
BsrFI r'CCGG_y
Cuts at: 0 156 406 1404
Size: 156 250 998
Fragments arranged by size:
998 250 156
BstEII G'GTnAC_C
Cuts at: 0 934 1404
Size: 934 470
BstXI CCAn_nnnn'nTGG
Cuts at: 0 624 748 1404
Size: 624 124 656
Fragments arranged by size:
656 624 124
BstYI r'GATC_y
Cuts at: 0 891 1212 1404
Size: 891 321 192
Fragments arranged by size:
891 321 192
BtgI C'CryG_G
Cuts at: 0 552 1160 1188 1404
Size: 552 608 28 216
Fragments arranged by size:
608 552 216 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 134 1404
Size: 134 1270
DraI TTT'AAA
Cuts at: 0 737 774 1404
Size: 737 37 630
Fragments arranged by size:
737 630 37
DraIII CAC_nnn'GTG
Cuts at: 0 810 1404
Size: 810 594
EaeI y'GGCC_r
Cuts at: 0 1217 1404
Size: 1217 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 616 1404
Size: 616 788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 223 241 364 646 1074 1150 1261
Size: 168 55 18 123 282 428 76 111
Cuts at: 1261 1404
Size: 143
Fragments arranged by size:
428 282 168 143 123 111 76 55
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 946 1404
Size: 946 458
EcoO109I rG'GnC_Cy
Cuts at: 0 113 320 1337 1404
Size: 113 207 1017 67
Fragments arranged by size:
1017 207 113 67
EcoRI G'AATT_C
Cuts at: 0 16 131 1404
Size: 16 115 1273
Fragments arranged by size:
1273 115 16
EcoRV GAT'ATC
Cuts at: 0 1398 1404
Size: 1398 6
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 864 1404
Size: 864 540
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 602 634 952 984 1404
Size: 602 32 318 32 420
Fragments arranged by size:
602 420 318 32 32
MscI TGG'CCA
Cuts at: 0 1219 1404
Size: 1219 185
MslI CAynn'nnrTG
Cuts at: 0 58 758 1404
Size: 58 700 646
Fragments arranged by size:
700 646 58
MspA1I CmG'CkG
Cuts at: 0 279 1190 1404
Size: 279 911 214
Fragments arranged by size:
911 279 214
NaeI GCC'GGC
Cuts at: 0 158 1404
Size: 158 1246
NcoI C'CATG_G
Cuts at: 0 552 1404
Size: 552 852
NgoMIV G'CCGG_C
Cuts at: 0 156 1404
Size: 156 1248
PflMI CCAn_nnn'nTGG
Cuts at: 0 342 1404
Size: 342 1062
PfoI T'CCnGG_A
Cuts at: 0 970 1404
Size: 970 434
PmlI CAC'GTG
Cuts at: 0 303 1404
Size: 303 1101
PpuMI rG'GwC_Cy
Cuts at: 0 1337 1404
Size: 1337 67
SacII CC_GC'GG
Cuts at: 0 1191 1404
Size: 1191 213
SanDI GG'GwC_CC
Cuts at: 0 1337 1404
Size: 1337 67
SmlI C'TyrA_G
Cuts at: 0 119 574 1404
Size: 119 455 830
Fragments arranged by size:
830 455 119
StyI C'CwwG_G
Cuts at: 0 552 1277 1404
Size: 552 725 127
Fragments arranged by size:
725 552 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1173 1404
Size: 1173 231
TatI w'GTAC_w
Cuts at: 0 290 1404
Size: 290 1114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 865 897 1276 1308 1404
Size: 307 32 526 32 379 32 96
Fragments arranged by size:
526 379 307 96 32 32 32
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApoI AseI AvaI
BanI BanII BbsI BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BstEII BstXI BstYI BtgI
BtgZI DraI DraIII EaeI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI Hin4I MscI MslI MspA1I NaeI
NcoI NgoMIV PflMI PfoI PmlI PpuMI SacII SanDI
SmlI StyI TaqII TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmtI Bpu10I BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtsI ClaI CspCI
DrdI EagI EarI EcoICRI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI