Restriction map for
pF1KSDA0775
(Linear) (Six-base) MAPSORT of: KIAA0775.seq Check: 801 from: 1 to: 1845
KIAA0775.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 482 1845
Size: 482 1363
AccI GT'mk_AC
Cuts at: 0 212 1845
Size: 212 1633
Acc65I G'GTAC_C
Cuts at: 0 1787 1845
Size: 1787 58
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1845
Size: 802 1043
AflIII A'CryG_T
Cuts at: 0 104 1845
Size: 104 1741
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1555 1845
Size: 1555 290
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 1845
Size: 27 1818
AlwNI CAG_nnn'CTG
Cuts at: 0 504 813 1845
Size: 504 309 1032
Fragments arranged by size:
1032 504 309
ApaI G_GGCC'C
Cuts at: 0 459 807 1845
Size: 459 348 1038
Fragments arranged by size:
1038 459 348
ApoI r'AATT_y
Cuts at: 0 876 1845
Size: 876 969
AscI GG'CGCG_CC
Cuts at: 0 1518 1845
Size: 1518 327
AvaI C'yCGr_G
Cuts at: 0 719 800 1061 1466 1654 1845
Size: 719 81 261 405 188 191
Fragments arranged by size:
719 405 261 191 188 81
BanI G'GyrC_C
Cuts at: 0 1203 1248 1417 1612 1787 1845
Size: 1203 45 169 195 175 58
Fragments arranged by size:
1203 195 175 169 58 45
BanII G_rGCy'C
Cuts at: 0 459 553 649 729 807 1487 1741 1845
Size: 459 94 96 80 78 680 254 104
Fragments arranged by size:
680 459 254 104 96 94 80 78
BbeI G_GCGC'C
Cuts at: 0 1421 1845
Size: 1421 424
BbvCI CC'TCA_GC
Cuts at: 0 442 1507 1845
Size: 442 1065 338
Fragments arranged by size:
1065 442 338
BclI T'GATC_A
Cuts at: 0 233 867 1845
Size: 233 634 978
Fragments arranged by size:
978 634 233
BglI GCCn_nnn'nGGC
Cuts at: 0 724 1066 1447 1845
Size: 724 342 381 398
Fragments arranged by size:
724 398 381 342
BglII A'GATC_T
Cuts at: 0 482 1845
Size: 482 1363
BlpI GC'TnA_GC
Cuts at: 0 728 1845
Size: 728 1117
Bme1580I G_kGCm'C
Cuts at: 0 459 807 1042 1081 1124 1132 1253 1586
Size: 459 348 235 39 43 8 121 333
Cuts at: 1586 1615 1845
Size: 29 230
Fragments arranged by size:
459 348 333 235 230 121 43 39
29 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 650 1029 1845
Size: 650 379 816
Fragments arranged by size:
816 650 379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 316 508 691 733 1845
Size: 193 123 192 183 42 1112
Fragments arranged by size:
1112 193 192 183 123 42
Bpu10I CC'TnA_GC
Cuts at: 0 442 547 1507 1845
Size: 442 105 960 338
Fragments arranged by size:
960 442 338 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 645 975 1845
Size: 645 330 870
Fragments arranged by size:
870 645 330
BsaI GGTCTCn'nnnn_
Cuts at: 0 77 790 1845
Size: 77 713 1055
Fragments arranged by size:
1055 713 77
BsaAI yAC'GTr
Cuts at: 0 1353 1845
Size: 1353 492
BsaHI Gr'CG_yC
Cuts at: 0 198 1418 1845
Size: 198 1220 427
Fragments arranged by size:
1220 427 198
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 94 124 1558 1588 1804 1834 1845
Size: 25 69 30 1434 30 216 30 11
Fragments arranged by size:
1434 216 69 30 30 30 25 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 754 922 1579 1845
Size: 265 489 168 657 266
Fragments arranged by size:
657 489 266 265 168
BseYI C'CCAG_C
Cuts at: 0 162 912 1103 1225 1255 1342 1597 1770
Size: 162 750 191 122 30 87 255 173
Cuts at: 1770 1845
Size: 75
Fragments arranged by size:
750 255 191 173 162 122 87 75
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 577 1845
Size: 561 16 1268
Fragments arranged by size:
1268 561 16
BsiHKAI G_wGCw'C
Cuts at: 0 112 1487 1694 1845
Size: 112 1375 207 151
Fragments arranged by size:
1375 207 151 112
BsmBI CGTCTCn'nnnn_
Cuts at: 0 460 1723 1845
Size: 460 1263 122
Fragments arranged by size:
1263 460 122
Bsp1286I G_dGCh'C
Cuts at: 0 112 459 553 649 729 807 1042 1081
Size: 112 347 94 96 80 78 235 39
Cuts at: 1081 1124 1132 1253 1487 1586 1615 1694 1741
Size: 43 8 121 234 99 29 79 47
Cuts at: 1741 1845
Size: 104
Fragments arranged by size:
347 235 234 121 112 104 99 96
94 80 79 78 47 43 39 29
8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 482 599 1845
Size: 482 117 1246
Fragments arranged by size:
1246 482 117
BsrBI CCG'CTC
Cuts at: 0 675 765 1066 1112 1151 1196 1845
Size: 675 90 301 46 39 45 649
Fragments arranged by size:
675 649 301 90 46 45 39
BsrFI r'CCGG_y
Cuts at: 0 917 1200 1360 1845
Size: 917 283 160 485
Fragments arranged by size:
917 485 283 160
BssHII G'CGCG_C
Cuts at: 0 1323 1518 1845
Size: 1323 195 327
Fragments arranged by size:
1323 327 195
BstAPI GCAn_nnn'nTGC
Cuts at: 0 504 1845
Size: 504 1341
BstYI r'GATC_y
Cuts at: 0 482 616 810 1845
Size: 482 134 194 1035
Fragments arranged by size:
1035 482 194 134
BtgI C'CryG_G
Cuts at: 0 1513 1845
Size: 1513 332
BtsI GCAGTG_nn'
Cuts at: 0 134 1845
Size: 134 1711
DraIII CAC_nnn'GTG
Cuts at: 0 658 1353 1845
Size: 658 695 492
Fragments arranged by size:
695 658 492
EaeI y'GGCC_r
Cuts at: 0 640 990 1279 1316 1448 1705 1845
Size: 640 350 289 37 132 257 140
Fragments arranged by size:
640 350 289 257 140 132 37
EarI CTCTTCn'nnn_
Cuts at: 0 415 683 1845
Size: 415 268 1162
Fragments arranged by size:
1162 415 268
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 102 1411 1845
Size: 102 1309 434
Fragments arranged by size:
1309 434 102
EcoICRI GAG'CTC
Cuts at: 0 1485 1845
Size: 1485 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 316 508 691 733 802 1845
Size: 193 123 192 183 42 69 1043
Fragments arranged by size:
1043 193 192 183 123 69 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 37 295 1845
Size: 37 258 1550
Fragments arranged by size:
1550 258 37
EcoO109I rG'GnC_Cy
Cuts at: 0 45 52 168 455 456 1502 1845
Size: 45 7 116 287 1 1046 343
Fragments arranged by size:
1046 343 287 116 45 7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 394 444 1380 1845
Size: 394 50 936 465
Fragments arranged by size:
936 465 394 50
HaeII r_GCGC'y
Cuts at: 0 886 1110 1149 1194 1239 1421 1845
Size: 886 224 39 45 45 182 424
Fragments arranged by size:
886 424 224 182 45 45 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 188 220 374 406 802 834
Size: 94 32 62 32 154 32 396 32
Cuts at: 834 1845
Size: 1011
Fragments arranged by size:
1011 396 154 94 62 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 1417 1845
Size: 1417 428
KpnI G_GTAC'C
Cuts at: 0 1791 1845
Size: 1791 54
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 566 809 845 1541 1766 1845
Size: 219 347 243 36 696 225 79
Fragments arranged by size:
696 347 243 225 219 79 36
MspA1I CmG'CkG
Cuts at: 0 504 789 1008 1259 1515 1622 1754 1770
Size: 504 285 219 251 256 107 132 16
Cuts at: 1770 1845
Size: 75
Fragments arranged by size:
504 285 256 251 219 132 107 75
16
NaeI GCC'GGC
Cuts at: 0 919 1362 1845
Size: 919 443 483
Fragments arranged by size:
919 483 443
NarI GG'CG_CC
Cuts at: 0 1418 1845
Size: 1418 427
NgoMIV G'CCGG_C
Cuts at: 0 917 1360 1845
Size: 917 443 485
Fragments arranged by size:
917 485 443
NspI r_CATG'y
Cuts at: 0 108 1845
Size: 108 1737
PasI CC'CwG_GG
Cuts at: 0 48 1845
Size: 48 1797
PciI A'CATG_T
Cuts at: 0 104 1845
Size: 104 1741
PflMI CCAn_nnn'nTGG
Cuts at: 0 813 1845
Size: 813 1032
PfoI T'CCnGG_A
Cuts at: 0 78 524 734 1845
Size: 78 446 210 1111
Fragments arranged by size:
1111 446 210 78
PmlI CAC'GTG
Cuts at: 0 1353 1845
Size: 1353 492
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1845
Size: 27 1818
PspOMI G'GGCC_C
Cuts at: 0 455 803 1845
Size: 455 348 1042
Fragments arranged by size:
1042 455 348
PstI C_TGCA'G
Cuts at: 0 320 773 794 1439 1845
Size: 320 453 21 645 406
Fragments arranged by size:
645 453 406 320 21
PvuII CAG'CTG
Cuts at: 0 504 789 1259 1754 1770 1845
Size: 504 285 470 495 16 75
Fragments arranged by size:
504 495 470 285 75 16
SacI G_AGCT'C
Cuts at: 0 1487 1845
Size: 1487 358
SacII CC_GC'GG
Cuts at: 0 1516 1845
Size: 1516 329
SfcI C'TryA_G
Cuts at: 0 316 769 790 940 1435 1845
Size: 316 453 21 150 495 410
Fragments arranged by size:
495 453 410 316 150 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1447 1845
Size: 1447 398
SfoI GGC'GCC
Cuts at: 0 1419 1845
Size: 1419 426
SgrAI Cr'CCGG_yG
Cuts at: 0 1200 1845
Size: 1200 645
SmaI CCC'GGG
Cuts at: 0 721 802 1468 1845
Size: 721 81 666 377
Fragments arranged by size:
721 666 377 81
SmlI C'TyrA_G
Cuts at: 0 624 954 1845
Size: 624 330 891
Fragments arranged by size:
891 624 330
StuI AGG'CCT
Cuts at: 0 397 521 1845
Size: 397 124 1324
Fragments arranged by size:
1324 397 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 669 837 1845
Size: 669 168 1008
Fragments arranged by size:
1008 669 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1558 1590 1684 1716 1804 1836 1845
Size: 1558 32 94 32 88 32 9
Fragments arranged by size:
1558 94 88 32 32 32 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1605 1845
Size: 1605 240
XmaI C'CCGG_G
Cuts at: 0 719 800 1466 1845
Size: 719 81 666 379
Fragments arranged by size:
719 666 379 81
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AloI AlwNI
ApaI ApoI AscI AvaI BanI BanII BbeI BbvCI
BclI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI
BstYI BtgI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI
MmeI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpiI PspOMI PstI PvuII SacI
SacII SfcI SfiI SfoI SgrAI SmaI SmlI StuI
TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI ApaLI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BfrBI BmgBI BmtI BplI BsaBI BsaWI BsiEI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EagI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MscI MslI NcoI NdeI NheI
NotI NruI NsiI PacI PmeI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
ScaI SexAI SgfI SnaBI SpeI SphI SrfI SspI
StyI SwaI TatI Tth111I XbaI XhoI XmnI ZraI