Restriction map for
pF1KSDA0778
(Linear) (Six-base) MAPSORT of: KIAA0778.seq Check: 25 from: 1 to: 3060
KIAA0778.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 39 3060
Size: 39 3021
AatII G_ACGT'C
Cuts at: 0 2213 3060
Size: 2213 847
Acc65I G'GTAC_C
Cuts at: 0 486 3060
Size: 486 2574
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 2020 3060
Size: 118 1902 1040
Fragments arranged by size:
1902 1040 118
AflIII A'CryG_T
Cuts at: 0 1163 3060
Size: 1163 1897
AgeI A'CCGG_T
Cuts at: 0 1538 3060
Size: 1538 1522
AleI CACnn'nnGTG
Cuts at: 0 2272 3060
Size: 2272 788
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 851 883 3060
Size: 851 32 2177
Fragments arranged by size:
2177 851 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1795 2577 3060
Size: 1795 782 483
Fragments arranged by size:
1795 782 483
ApaI G_GGCC'C
Cuts at: 0 224 1521 3060
Size: 224 1297 1539
Fragments arranged by size:
1539 1297 224
ApaLI G'TGCA_C
Cuts at: 0 1984 3060
Size: 1984 1076
ApoI r'AATT_y
Cuts at: 0 1699 3060
Size: 1699 1361
AvaI C'yCGr_G
Cuts at: 0 212 679 1688 1775 2344 3060
Size: 212 467 1009 87 569 716
Fragments arranged by size:
1009 716 569 467 212 87
BanI G'GyrC_C
Cuts at: 0 486 1129 3060
Size: 486 643 1931
Fragments arranged by size:
1931 643 486
BanII G_rGCy'C
Cuts at: 0 198 224 457 665 1099 1379 1521 1754
Size: 198 26 233 208 434 280 142 233
Cuts at: 1754 2864 3060
Size: 1110 196
Fragments arranged by size:
1110 434 280 233 233 208 198 196
142 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 1281 1487 2814 3060
Size: 1281 206 1327 246
Fragments arranged by size:
1327 1281 246 206
BbvCI CC'TCA_GC
Cuts at: 0 491 3060
Size: 491 2569
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1137 1171 2140 2174 3060
Size: 1137 34 969 34 886
Fragments arranged by size:
1137 969 886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3035 3060
Size: 3035 25
BclI T'GATC_A
Cuts at: 0 875 1829 3060
Size: 875 954 1231
Fragments arranged by size:
1231 954 875
BglI GCCn_nnn'nGGC
Cuts at: 0 43 996 2125 2464 2634 3060
Size: 43 953 1129 339 170 426
Fragments arranged by size:
1129 953 426 339 170 43
Bme1580I G_kGCm'C
Cuts at: 0 224 1132 1521 1988 2401 2431 3060
Size: 224 908 389 467 413 30 629
Fragments arranged by size:
908 629 467 413 389 224 30
BmgBI CAC'GTC
Cuts at: 0 1103 3060
Size: 1103 1957
BmrI ACTGGGnnnn_n'
Cuts at: 0 2587 2650 2667 3060
Size: 2587 63 17 393
Fragments arranged by size:
2587 393 63 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1329 3060
Size: 1329 1731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 844 967 1096 2704 2887 3060
Size: 724 120 123 129 1608 183 173
Fragments arranged by size:
1608 724 183 173 129 123 120
Bpu10I CC'TnA_GC
Cuts at: 0 197 491 3060
Size: 197 294 2569
Fragments arranged by size:
2569 294 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1348 2020 3060
Size: 1348 672 1040
Fragments arranged by size:
1348 1040 672
BsaI GGTCTCn'nnnn_
Cuts at: 0 551 575 700 773 909 1276 3060
Size: 551 24 125 73 136 367 1784
Fragments arranged by size:
1784 551 367 136 125 73 24
BsaAI yAC'GTr
Cuts at: 0 1244 1500 2741 3060
Size: 1244 256 1241 319
Fragments arranged by size:
1244 1241 319 256
BsaBI GATnn'nnATC
Cuts at: 0 529 3060
Size: 529 2531
BsaHI Gr'CG_yC
Cuts at: 0 2210 3060
Size: 2210 850
BsaWI w'CCGG_w
Cuts at: 0 602 782 1538 2666 3060
Size: 602 180 756 1128 394
Fragments arranged by size:
1128 756 602 394 180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 225 255 548 572 578 602 1528 1558
Size: 225 30 293 24 6 24 926 30
Cuts at: 1558 2108 2138 2242 2272 2725 2755 2863 2893
Size: 550 30 104 30 453 30 108 30
Cuts at: 2893 3060
Size: 167
Fragments arranged by size:
926 550 453 293 225 167 108 104
30 30 30 30 30 30 24 24
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2293 2884 2968 3060
Size: 2293 591 84 92
Fragments arranged by size:
2293 591 92 84
BseYI C'CCAG_C
Cuts at: 0 144 1092 1623 1647 2075 3035 3060
Size: 144 948 531 24 428 960 25
Fragments arranged by size:
960 948 531 428 144 25 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1579 2797 3060
Size: 1579 1218 263
Fragments arranged by size:
1579 1218 263
BsiHKAI G_wGCw'C
Cuts at: 0 457 920 1379 1546 1988 3060
Size: 457 463 459 167 442 1072
Fragments arranged by size:
1072 463 459 457 442 167
BsmI GAATG_Cn'
Cuts at: 0 1529 2752 3060
Size: 1529 1223 308
Fragments arranged by size:
1529 1223 308
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1081 2075 2217 2866 3060
Size: 1081 994 142 649 194
Fragments arranged by size:
1081 994 649 194 142
Bsp1286I G_dGCh'C
Cuts at: 0 198 224 457 665 920 1099 1132 1379
Size: 198 26 233 208 255 179 33 247
Cuts at: 1379 1521 1546 1754 1988 2401 2431 2864 3060
Size: 142 25 208 234 413 30 433 196
Fragments arranged by size:
433 413 255 247 234 233 208 208
198 196 179 142 33 30 26 25
BspEI T'CCGG_A
Cuts at: 0 602 3060
Size: 602 2458
BspHI T'CATG_A
Cuts at: 0 2483 3060
Size: 2483 577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 39 3060
Size: 39 3021
BsrBI CCG'CTC
Cuts at: 0 674 1578 2937 3060
Size: 674 904 1359 123
Fragments arranged by size:
1359 904 674 123
BsrDI GCAATG_nn'
Cuts at: 0 119 854 1868 1919 2183 2372 3060
Size: 119 735 1014 51 264 189 688
Fragments arranged by size:
1014 735 688 264 189 119 51
BsrFI r'CCGG_y
Cuts at: 0 1538 3060
Size: 1538 1522
BssSI C'ACGA_G
Cuts at: 0 1471 3060
Size: 1471 1589
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2758 3060
Size: 2758 302
BstEII G'GTnAC_C
Cuts at: 0 1836 3060
Size: 1836 1224
BstXI CCAn_nnnn'nTGG
Cuts at: 0 465 849 1044 1554 1881 2454 2769 3060
Size: 465 384 195 510 327 573 315 291
Fragments arranged by size:
573 510 465 384 327 315 291 195
BstYI r'GATC_y
Cuts at: 0 1571 2030 2846 3060
Size: 1571 459 816 214
Fragments arranged by size:
1571 816 459 214
Bsu36I CC'TnA_GG
Cuts at: 0 816 992 3060
Size: 816 176 2068
Fragments arranged by size:
2068 816 176
BtgI C'CryG_G
Cuts at: 0 6 41 353 1727 2189 3060
Size: 6 35 312 1374 462 871
Fragments arranged by size:
1374 871 462 312 35 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2301 3060
Size: 2301 759
BtsI GCAGTG_nn'
Cuts at: 0 749 2785 3060
Size: 749 2036 275
Fragments arranged by size:
2036 749 275
DraIII CAC_nnn'GTG
Cuts at: 0 1019 2581 2951 3060
Size: 1019 1562 370 109
Fragments arranged by size:
1562 1019 370 109
DrdI GACnn_nn'nnGTC
Cuts at: 0 1254 3060
Size: 1254 1806
EaeI y'GGCC_r
Cuts at: 0 15 980 1004 2126 3060
Size: 15 965 24 1122 934
Fragments arranged by size:
1122 965 934 24 15
EarI CTCTTCn'nnn_
Cuts at: 0 618 3060
Size: 618 2442
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 849 2638 3060
Size: 849 1789 422
Fragments arranged by size:
1789 849 422
EcoICRI GAG'CTC
Cuts at: 0 455 1377 3060
Size: 455 922 1683
Fragments arranged by size:
1683 922 455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 724 844 967 1096 2020 2704 2887
Size: 118 606 120 123 129 924 684 183
Cuts at: 2887 3060
Size: 173
Fragments arranged by size:
924 684 606 183 173 129 123 120
118
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 565 1558 3060
Size: 172 393 993 1502
Fragments arranged by size:
1502 993 393 172
EcoO109I rG'GnC_Cy
Cuts at: 0 177 1517 1518 3060
Size: 177 1340 1 1542
Fragments arranged by size:
1542 1340 177 1
EcoRV GAT'ATC
Cuts at: 0 2481 3060
Size: 2481 579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 150 1758 3060
Size: 150 1608 1302
Fragments arranged by size:
1608 1302 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 572 604 625 657 1219 1251
Size: 352 32 188 32 21 32 562 32
Cuts at: 1251 1528 1560 1755 1787 1996 2028 3060
Size: 277 32 195 32 209 32 1032
Fragments arranged by size:
1032 562 352 277 209 195 188 32
32 32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 0 1660 1956 3060
Size: 1660 296 1104
Fragments arranged by size:
1660 1104 296
HindIII A'AGCT_T
Cuts at: 0 1735 3060
Size: 1735 1325
KpnI G_GTAC'C
Cuts at: 0 490 3060
Size: 490 2570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 638 3060
Size: 395 243 2422
Fragments arranged by size:
2422 395 243
MscI TGG'CCA
Cuts at: 0 982 1006 3060
Size: 982 24 2054
Fragments arranged by size:
2054 982 24
MslI CAynn'nnrTG
Cuts at: 0 847 976 1315 1555 1759 1825 1942 2092
Size: 847 129 339 240 204 66 117 150
Cuts at: 2092 2188 2272 2434 2542 2770 3060
Size: 96 84 162 108 228 290
Fragments arranged by size:
847 339 290 240 228 204 162 150
129 117 108 96 84 66
MspA1I CmG'CkG
Cuts at: 0 192 406 873 941 1336 1461 2470 2721
Size: 192 214 467 68 395 125 1009 251
Cuts at: 2721 3060
Size: 339
Fragments arranged by size:
1009 467 395 339 251 214 192 125
68
NcoI C'CATG_G
Cuts at: 0 353 2189 3060
Size: 353 1836 871
Fragments arranged by size:
1836 871 353
NspI r_CATG'y
Cuts at: 0 1167 1979 2931 3060
Size: 1167 812 952 129
Fragments arranged by size:
1167 952 812 129
PciI A'CATG_T
Cuts at: 0 1163 3060
Size: 1163 1897
PflMI CCAn_nnn'nTGG
Cuts at: 0 1503 2577 3060
Size: 1503 1074 483
Fragments arranged by size:
1503 1074 483
PfoI T'CCnGG_A
Cuts at: 0 509 2864 3060
Size: 509 2355 196
Fragments arranged by size:
2355 509 196
PmlI CAC'GTG
Cuts at: 0 1500 2741 3060
Size: 1500 1241 319
Fragments arranged by size:
1500 1241 319
PspOMI G'GGCC_C
Cuts at: 0 220 1517 3060
Size: 220 1297 1543
Fragments arranged by size:
1543 1297 220
PvuII CAG'CTG
Cuts at: 0 406 873 941 1336 1461 2470 3060
Size: 406 467 68 395 125 1009 590
Fragments arranged by size:
1009 590 467 406 395 125 68
SacI G_AGCT'C
Cuts at: 0 457 1379 3060
Size: 457 922 1681
Fragments arranged by size:
1681 922 457
ScaI AGT'ACT
Cuts at: 0 25 3060
Size: 25 3035
SexAI A'CCwGG_T
Cuts at: 0 560 2947 3060
Size: 560 2387 113
Fragments arranged by size:
2387 560 113
SfcI C'TryA_G
Cuts at: 0 936 2970 3060
Size: 936 2034 90
Fragments arranged by size:
2034 936 90
SmaI CCC'GGG
Cuts at: 0 1777 3060
Size: 1777 1283
SmlI C'TyrA_G
Cuts at: 0 212 1363 2035 3060
Size: 212 1151 672 1025
Fragments arranged by size:
1151 1025 672 212
SphI G_CATG'C
Cuts at: 0 1979 3060
Size: 1979 1081
StuI AGG'CCT
Cuts at: 0 821 3060
Size: 821 2239
StyI C'CwwG_G
Cuts at: 0 134 173 353 1418 1862 1970 2189 2453
Size: 134 39 180 1065 444 108 219 264
Cuts at: 2453 3060
Size: 607
Fragments arranged by size:
1065 607 444 264 219 180 134 108
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 379 703 855 1153 2569 2902 3027 3060
Size: 379 324 152 298 1416 333 125 33
Fragments arranged by size:
1416 379 333 324 298 152 125 33
TatI w'GTAC_w
Cuts at: 0 23 3060
Size: 23 3037
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 115 147 225 257 548 580 2108 2140
Size: 115 32 78 32 291 32 1528 32
Cuts at: 2140 2725 2757 3060
Size: 585 32 303
Fragments arranged by size:
1528 585 303 291 115 78 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 586 3060
Size: 586 2474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1977 2578 2679 2769 3060
Size: 1977 601 101 90 291
Fragments arranged by size:
1977 601 291 101 90
XhoI C'TCGA_G
Cuts at: 0 212 3060
Size: 212 2848
XmaI C'CCGG_G
Cuts at: 0 1775 3060
Size: 1775 1285
XmnI GAAnn'nnTTC
Cuts at: 0 2620 3060
Size: 2620 440
ZraI GAC'GTC
Cuts at: 0 2211 3060
Size: 2211 849
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI Hin4I HincII HindIII KpnI MmeI
MscI MslI MspA1I NcoI NspI PciI PflMI PfoI
PmlI PspOMI PvuII SacI ScaI SexAI SfcI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AhdI AlfI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BfrBI BglII BlpI
BmtI BsiEI BsiWI BsrGI BssHII BstBI BstZ17I ClaI
CspCI DraI EagI EcoRI FseI FspI FspAI HaeII
HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI XbaI