Restriction map for
pF1KSDA0780
(Linear) (Six-base) MAPSORT of: KIAA0780.seq Check: 7543 from: 1 to: 3141
KIAA0780.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1305 2817 3141
Size: 1305 1512 324
Fragments arranged by size:
1512 1305 324
AccI GT'mk_AC
Cuts at: 0 1132 1471 3141
Size: 1132 339 1670
Fragments arranged by size:
1670 1132 339
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 1082 1268 2815 3141
Size: 39 1043 186 1547 326
Fragments arranged by size:
1547 1043 326 186 39
AflII C'TTAA_G
Cuts at: 0 1365 2293 3141
Size: 1365 928 848
Fragments arranged by size:
1365 928 848
AflIII A'CryG_T
Cuts at: 0 2891 3141
Size: 2891 250
AhdI GACnn_n'nnGTC
Cuts at: 0 2593 3141
Size: 2593 548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2178 3141
Size: 2178 963
ApaI G_GGCC'C
Cuts at: 0 2322 2806 3141
Size: 2322 484 335
Fragments arranged by size:
2322 484 335
ApaLI G'TGCA_C
Cuts at: 0 921 3141
Size: 921 2220
ApoI r'AATT_y
Cuts at: 0 802 873 973 1326 3033 3141
Size: 802 71 100 353 1707 108
Fragments arranged by size:
1707 802 353 108 100 71
AseI AT'TA_AT
Cuts at: 0 413 596 3095 3141
Size: 413 183 2499 46
Fragments arranged by size:
2499 413 183 46
AvaI C'yCGr_G
Cuts at: 0 1489 3141
Size: 1489 1652
BamHI G'GATC_C
Cuts at: 0 2917 3141
Size: 2917 224
BanI G'GyrC_C
Cuts at: 0 390 1712 1770 3141
Size: 390 1322 58 1371
Fragments arranged by size:
1371 1322 390 58
BanII G_rGCy'C
Cuts at: 0 123 620 2322 2806 3141
Size: 123 497 1702 484 335
Fragments arranged by size:
1702 497 484 335 123
BbeI G_GCGC'C
Cuts at: 0 1716 1774 3141
Size: 1716 58 1367
Fragments arranged by size:
1716 1367 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 556 581 1391 3141
Size: 556 25 810 1750
Fragments arranged by size:
1750 810 556 25
BbvCI CC'TCA_GC
Cuts at: 0 1264 2813 3141
Size: 1264 1549 328
Fragments arranged by size:
1549 1264 328
BclI T'GATC_A
Cuts at: 0 809 1029 3141
Size: 809 220 2112
Fragments arranged by size:
2112 809 220
BfrBI ATG'CAT
Cuts at: 0 712 1417 2429 3141
Size: 712 705 1012 712
Fragments arranged by size:
1012 712 712 705
BglII A'GATC_T
Cuts at: 0 1741 2207 3141
Size: 1741 466 934
Fragments arranged by size:
1741 934 466
BlpI GC'TnA_GC
Cuts at: 0 1889 3141
Size: 1889 1252
Bme1580I G_kGCm'C
Cuts at: 0 925 2232 2322 2523 2806 3141
Size: 925 1307 90 201 283 335
Fragments arranged by size:
1307 925 335 283 201 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 1637 1667 3141
Size: 1637 30 1474
Fragments arranged by size:
1637 1474 30
BmtI G_CTAG'C
Cuts at: 0 1307 3141
Size: 1307 1834
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1557 3141
Size: 1557 1584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 1031 1718 2483 3141
Size: 818 213 687 765 658
Fragments arranged by size:
818 765 687 658 213
Bpu10I CC'TnA_GC
Cuts at: 0 1264 2813 3141
Size: 1264 1549 328
Fragments arranged by size:
1549 1264 328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 1213 3141
Size: 659 554 1928
Fragments arranged by size:
1928 659 554
BsaI GGTCTCn'nnnn_
Cuts at: 0 2463 3141
Size: 2463 678
BsaHI Gr'CG_yC
Cuts at: 0 1713 1771 3141
Size: 1713 58 1370
Fragments arranged by size:
1713 1370 58
BsaWI w'CCGG_w
Cuts at: 0 642 3141
Size: 642 2499
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 349 379 2810 2840 3141
Size: 349 30 2431 30 301
Fragments arranged by size:
2431 349 301 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 1098 1101 1825 2297 3141
Size: 85 1013 3 724 472 844
Fragments arranged by size:
1013 844 724 472 85 3
BseYI C'CCAG_C
Cuts at: 0 34 1632 2530 2799 3065 3141
Size: 34 1598 898 269 266 76
Fragments arranged by size:
1598 898 269 266 76 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 881 1926 3141
Size: 425 456 1045 1215
Fragments arranged by size:
1215 1045 456 425
BsiEI CG_ry'CG
Cuts at: 0 2490 3141
Size: 2490 651
BsiHKAI G_wGCw'C
Cuts at: 0 925 2292 3141
Size: 925 1367 849
Fragments arranged by size:
1367 925 849
BsiWI C'GTAC_G
Cuts at: 0 2166 3141
Size: 2166 975
BsmI GAATG_Cn'
Cuts at: 0 2266 3141
Size: 2266 875
Bsp1286I G_dGCh'C
Cuts at: 0 123 620 925 2232 2292 2322 2523 2806
Size: 123 497 305 1307 60 30 201 283
Cuts at: 2806 3141
Size: 335
Fragments arranged by size:
1307 497 335 305 283 201 123 60
30
BspEI T'CCGG_A
Cuts at: 0 642 3141
Size: 642 2499
BspHI T'CATG_A
Cuts at: 0 806 2202 3141
Size: 806 1396 939
Fragments arranged by size:
1396 939 806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1305 2817 3031 3141
Size: 1305 1512 214 110
Fragments arranged by size:
1512 1305 214 110
BsrDI GCAATG_nn'
Cuts at: 0 1986 2936 3141
Size: 1986 950 205
Fragments arranged by size:
1986 950 205
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2435 3141
Size: 2435 706
BstXI CCAn_nnnn'nTGG
Cuts at: 0 796 1609 2530 3141
Size: 796 813 921 611
Fragments arranged by size:
921 813 796 611
BstYI r'GATC_y
Cuts at: 0 1741 2207 2917 3141
Size: 1741 466 710 224
Fragments arranged by size:
1741 710 466 224
Bsu36I CC'TnA_GG
Cuts at: 0 155 1148 1793 2768 3141
Size: 155 993 645 975 373
Fragments arranged by size:
993 975 645 373 155
BtgI C'CryG_G
Cuts at: 0 65 1602 2639 3141
Size: 65 1537 1037 502
Fragments arranged by size:
1537 1037 502 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 292 2693 3141
Size: 292 2401 448
Fragments arranged by size:
2401 448 292
BtsI GCAGTG_nn'
Cuts at: 0 1262 3141
Size: 1262 1879
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2818 2853 3141
Size: 2818 35 288
Fragments arranged by size:
2818 288 35
DraIII CAC_nnn'GTG
Cuts at: 0 1933 3141
Size: 1933 1208
DrdI GACnn_nn'nnGTC
Cuts at: 0 1231 3141
Size: 1231 1910
EaeI y'GGCC_r
Cuts at: 0 8 305 1519 1913 3141
Size: 8 297 1214 394 1228
Fragments arranged by size:
1228 1214 394 297 8
EarI CTCTTCn'nnn_
Cuts at: 0 485 1157 1286 1577 2117 2929 2957 3141
Size: 485 672 129 291 540 812 28 184
Fragments arranged by size:
812 672 540 485 291 184 129 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 818 1031 1082 1268 1718 2483 2815
Size: 39 779 213 51 186 450 765 332
Cuts at: 2815 3141
Size: 326
Fragments arranged by size:
779 765 450 332 326 213 186 51
39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1140 2122 3141
Size: 1140 982 1019
Fragments arranged by size:
1140 1019 982
EcoO109I rG'GnC_Cy
Cuts at: 0 1197 3020 3141
Size: 1197 1823 121
Fragments arranged by size:
1823 1197 121
EcoRI G'AATT_C
Cuts at: 0 802 3033 3141
Size: 802 2231 108
Fragments arranged by size:
2231 802 108
EcoRV GAT'ATC
Cuts at: 0 960 991 2775 3141
Size: 960 31 1784 366
Fragments arranged by size:
1784 960 366 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1448 3141
Size: 1448 1693
HaeII r_GCGC'y
Cuts at: 0 1716 1774 3141
Size: 1716 58 1367
Fragments arranged by size:
1716 1367 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 607 639 1156 1188 1786 1818 2456 2488
Size: 607 32 517 32 598 32 638 32
Cuts at: 2488 2579 2611 2728 2760 2810 2842 2930 2962
Size: 91 32 117 32 50 32 88 32
Cuts at: 2962 3141
Size: 179
Fragments arranged by size:
638 607 598 517 179 117 91 88
50 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1571 2598 3141
Size: 1571 1027 543
Fragments arranged by size:
1571 1027 543
HindIII A'AGCT_T
Cuts at: 0 1113 1409 3141
Size: 1113 296 1732
Fragments arranged by size:
1732 1113 296
HpaI GTT'AAC
Cuts at: 0 1571 3141
Size: 1571 1570
KasI G'GCGC_C
Cuts at: 0 1712 1770 3141
Size: 1712 58 1371
Fragments arranged by size:
1712 1371 58
MscI TGG'CCA
Cuts at: 0 307 1521 1915 3141
Size: 307 1214 394 1226
Fragments arranged by size:
1226 1214 394 307
MslI CAynn'nnrTG
Cuts at: 0 1607 2638 3141
Size: 1607 1031 503
Fragments arranged by size:
1607 1031 503
MspA1I CmG'CkG
Cuts at: 0 38 303 1361 1636 1888 3141
Size: 38 265 1058 275 252 1253
Fragments arranged by size:
1253 1058 275 265 252 38
NarI GG'CG_CC
Cuts at: 0 1713 1771 3141
Size: 1713 58 1370
Fragments arranged by size:
1713 1370 58
NcoI C'CATG_G
Cuts at: 0 65 1602 3141
Size: 65 1537 1539
Fragments arranged by size:
1539 1537 65
NdeI CA'TA_TG
Cuts at: 0 822 3141
Size: 822 2319
NheI G'CTAG_C
Cuts at: 0 1303 3141
Size: 1303 1838
NsiI A_TGCA'T
Cuts at: 0 714 1419 2431 3141
Size: 714 705 1012 710
Fragments arranged by size:
1012 714 710 705
NspI r_CATG'y
Cuts at: 0 546 2582 2895 3141
Size: 546 2036 313 246
Fragments arranged by size:
2036 546 313 246
PciI A'CATG_T
Cuts at: 0 2891 3141
Size: 2891 250
PflMI CCAn_nnn'nTGG
Cuts at: 0 626 3141
Size: 626 2515
PfoI T'CCnGG_A
Cuts at: 0 77 3141
Size: 77 3064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1684 1716 3141
Size: 1684 32 1425
Fragments arranged by size:
1684 1425 32
PpuMI rG'GwC_Cy
Cuts at: 0 1197 3020 3141
Size: 1197 1823 121
Fragments arranged by size:
1823 1197 121
PspOMI G'GGCC_C
Cuts at: 0 2318 2802 3141
Size: 2318 484 339
Fragments arranged by size:
2318 484 339
PstI C_TGCA'G
Cuts at: 0 1082 2440 3141
Size: 1082 1358 701
Fragments arranged by size:
1358 1082 701
PvuII CAG'CTG
Cuts at: 0 38 303 1361 1888 3141
Size: 38 265 1058 527 1253
Fragments arranged by size:
1253 1058 527 265 38
ScaI AGT'ACT
Cuts at: 0 367 3141
Size: 367 2774
SexAI A'CCwGG_T
Cuts at: 0 2897 3141
Size: 2897 244
SfcI C'TryA_G
Cuts at: 0 588 1078 2436 3141
Size: 588 490 1358 705
Fragments arranged by size:
1358 705 588 490
SfoI GGC'GCC
Cuts at: 0 1714 1772 3141
Size: 1714 58 1369
Fragments arranged by size:
1714 1369 58
SmlI C'TyrA_G
Cuts at: 0 674 1228 1365 2293 3141
Size: 674 554 137 928 848
Fragments arranged by size:
928 848 674 554 137
SspI AAT'ATT
Cuts at: 0 322 2098 2869 2883 3141
Size: 322 1776 771 14 258
Fragments arranged by size:
1776 771 322 258 14
StuI AGG'CCT
Cuts at: 0 1146 1731 3141
Size: 1146 585 1410
Fragments arranged by size:
1410 1146 585
StyI C'CwwG_G
Cuts at: 0 65 699 1602 2615 3141
Size: 65 634 903 1013 526
Fragments arranged by size:
1013 903 634 526 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2121 3141
Size: 2121 1020
TatI w'GTAC_w
Cuts at: 0 257 325 365 1369 3141
Size: 257 68 40 1004 1772
Fragments arranged by size:
1772 1004 257 68 40
Tth111I GACn'n_nGTC
Cuts at: 0 1213 1869 3141
Size: 1213 656 1272
Fragments arranged by size:
1272 1213 656
XmnI GAAnn'nnTTC
Cuts at: 0 292 3141
Size: 292 2849
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AhdI AlfI ApaI
ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrDI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII HpaI KasI MscI MslI MspA1I
NarI NcoI NdeI NheI NsiI NspI PciI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII ScaI SexAI
SfcI SfoI SmlI SspI StuI StyI TaqII TatI
Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AleI AloI AlwNI
AscI AsiSI AvrII BaeI BcgI BciVI BglI BmgBI
BsaAI BsaBI BsmBI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I ClaI DraI EagI EciI EcoICRI
FseI FspI FspAI KpnI MfeI MluI MmeI NaeI
NgoMIV NotI NruI PacI PasI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TstI XbaI XcmI XhoI
XmaI ZraI