Restriction map for
pF1KSDA0782
(Linear) (Six-base) MAPSORT of: KIAA0782.seq Check: 9090 from: 1 to: 3615
KIAA0782.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 341 3340 3615
Size: 341 2999 275
Fragments arranged by size:
2999 341 275
AccI GT'mk_AC
Cuts at: 0 862 3296 3615
Size: 862 2434 319
Fragments arranged by size:
2434 862 319
Acc65I G'GTAC_C
Cuts at: 0 2462 3386 3615
Size: 2462 924 229
Fragments arranged by size:
2462 924 229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 1054 3615
Size: 768 286 2561
Fragments arranged by size:
2561 768 286
AfeI AGC'GCT
Cuts at: 0 373 552 979 2362 3615
Size: 373 179 427 1383 1253
Fragments arranged by size:
1383 1253 427 373 179
AflIII A'CryG_T
Cuts at: 0 3483 3615
Size: 3483 132
AhdI GACnn_n'nnGTC
Cuts at: 0 2142 3594 3615
Size: 2142 1452 21
Fragments arranged by size:
2142 1452 21
AleI CACnn'nnGTG
Cuts at: 0 1432 3349 3615
Size: 1432 1917 266
Fragments arranged by size:
1917 1432 266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3211 3615
Size: 3211 404
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1897 1929 3615
Size: 1897 32 1686
Fragments arranged by size:
1897 1686 32
AlwNI CAG_nnn'CTG
Cuts at: 0 504 911 1395 2031 2274 2824 3191 3502
Size: 504 407 484 636 243 550 367 311
Cuts at: 3502 3615
Size: 113
Fragments arranged by size:
636 550 504 484 407 367 311 243
113
ApaI G_GGCC'C
Cuts at: 0 540 1560 1902 1961 2600 3243 3615
Size: 540 1020 342 59 639 643 372
Fragments arranged by size:
1020 643 639 540 372 342 59
ApaLI G'TGCA_C
Cuts at: 0 2308 2572 3615
Size: 2308 264 1043
Fragments arranged by size:
2308 1043 264
ApoI r'AATT_y
Cuts at: 0 1405 3615
Size: 1405 2210
AvaI C'yCGr_G
Cuts at: 0 533 1338 1559 1841 3491 3615
Size: 533 805 221 282 1650 124
Fragments arranged by size:
1650 805 533 282 221 124
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1729 1762 3615
Size: 1729 33 1853
Fragments arranged by size:
1853 1729 33
BanI G'GyrC_C
Cuts at: 0 1175 1434 2462 3386 3615
Size: 1175 259 1028 924 229
Fragments arranged by size:
1175 1028 924 259 229
BanII G_rGCy'C
Cuts at: 0 20 229 540 930 1076 1560 1902 1937
Size: 20 209 311 390 146 484 342 35
Cuts at: 1937 1961 2390 2600 2702 2870 3243 3416 3479
Size: 24 429 210 102 168 373 173 63
Cuts at: 3479 3615
Size: 136
Fragments arranged by size:
484 429 390 373 342 311 210 209
173 168 146 136 102 63 35 24
20
BbsI GAAGACnn'nnnn_
Cuts at: 0 1938 2269 2657 3615
Size: 1938 331 388 958
Fragments arranged by size:
1938 958 388 331
BbvCI CC'TCA_GC
Cuts at: 0 851 932 2861 3615
Size: 851 81 1929 754
Fragments arranged by size:
1929 851 754 81
BciVI GTATCCnnnnn_n'
Cuts at: 0 315 3615
Size: 315 3300
BclI T'GATC_A
Cuts at: 0 327 434 3615
Size: 327 107 3181
Fragments arranged by size:
3181 327 107
BglI GCCn_nnn'nGGC
Cuts at: 0 463 1505 2385 3052 3134 3615
Size: 463 1042 880 667 82 481
Fragments arranged by size:
1042 880 667 481 463 82
BlpI GC'TnA_GC
Cuts at: 0 64 521 3615
Size: 64 457 3094
Fragments arranged by size:
3094 457 64
Bme1580I G_kGCm'C
Cuts at: 0 534 540 1130 1560 1685 1902 1961 2312
Size: 534 6 590 430 125 217 59 351
Cuts at: 2312 2576 2600 3013 3243 3615
Size: 264 24 413 230 372
Fragments arranged by size:
590 534 430 413 372 351 264 230
217 125 59 24 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 951 1127 3615
Size: 951 176 2488
Fragments arranged by size:
2488 951 176
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2472 3615
Size: 2472 1143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1204 1780 2299 2440 2442 2896 3615
Size: 625 579 576 519 141 2 454 719
Fragments arranged by size:
719 625 579 576 519 454 141 2
Bpu10I CC'TnA_GC
Cuts at: 0 166 851 932 1606 1802 1885 2701 2861
Size: 166 685 81 674 196 83 816 160
Cuts at: 2861 3615
Size: 754
Fragments arranged by size:
816 754 685 674 196 166 160 83
81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2299 2341 3615
Size: 2299 42 1274
Fragments arranged by size:
2299 1274 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 1288 2460 3241 3615
Size: 1288 1172 781 374
Fragments arranged by size:
1288 1172 781 374
BsaAI yAC'GTr
Cuts at: 0 402 636 2334 3615
Size: 402 234 1698 1281
Fragments arranged by size:
1698 1281 402 234
BsaBI GATnn'nnATC
Cuts at: 0 326 3615
Size: 326 3289
BsaHI Gr'CG_yC
Cuts at: 0 134 1013 1863 3615
Size: 134 879 850 1752
Fragments arranged by size:
1752 879 850 134
BsaWI w'CCGG_w
Cuts at: 0 1421 3215 3615
Size: 1421 1794 400
Fragments arranged by size:
1794 1421 400
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 808 838 931 961 1177 1207 1897 1927
Size: 808 30 93 30 216 30 690 30
Cuts at: 1927 3615
Size: 1688
Fragments arranged by size:
1688 808 690 216 93 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 694 920 1258 2458 2461 2731 2878
Size: 28 666 226 338 1200 3 270 147
Cuts at: 2878 2965 3615
Size: 87 650
Fragments arranged by size:
1200 666 650 338 270 226 147 87
28 3
BseYI C'CCAG_C
Cuts at: 0 181 561 1086 1099 1150 1162 1321 1857
Size: 181 380 525 13 51 12 159 536
Cuts at: 1857 2100 2402 2768 2844 3006 3335 3513 3615
Size: 243 302 366 76 162 329 178 102
Fragments arranged by size:
536 525 380 366 329 302 243 181
178 162 159 102 76 51 13 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1004 2775 2953 2983 3466 3615
Size: 679 325 1771 178 30 483 149
Fragments arranged by size:
1771 679 483 325 178 149 30
BsiEI CG_ry'CG
Cuts at: 0 1493 3168 3615
Size: 1493 1675 447
Fragments arranged by size:
1675 1493 447
BsiHKAI G_wGCw'C
Cuts at: 0 995 1076 1712 1937 2312 2576 2702 2870
Size: 995 81 636 225 375 264 126 168
Cuts at: 2870 3416 3615
Size: 546 199
Fragments arranged by size:
995 636 546 375 264 225 199 168
126 81
BsmI GAATG_Cn'
Cuts at: 0 1807 3615
Size: 1807 1808
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1020 3615
Size: 1020 2595
Bsp1286I G_dGCh'C
Cuts at: 0 20 229 534 540 930 995 1076 1130
Size: 20 209 305 6 390 65 81 54
Cuts at: 1130 1560 1685 1712 1902 1937 1961 2312 2390
Size: 430 125 27 190 35 24 351 78
Cuts at: 2390 2576 2600 2702 2870 3013 3243 3416 3479
Size: 186 24 102 168 143 230 173 63
Cuts at: 3479 3615
Size: 136
Fragments arranged by size:
430 390 351 305 230 209 190 186
173 168 143 136 125 102 81 78
65 63 54 35 27 24 24 20
6
BspEI T'CCGG_A
Cuts at: 0 3215 3615
Size: 3215 400
BspHI T'CATG_A
Cuts at: 0 1779 3615
Size: 1779 1836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 2384 2935 3340 3615
Size: 341 2043 551 405 275
Fragments arranged by size:
2043 551 405 341 275
BsrBI CCG'CTC
Cuts at: 0 483 1227 1626 1854 2076 3236 3615
Size: 483 744 399 228 222 1160 379
Fragments arranged by size:
1160 744 483 399 379 228 222
BsrFI r'CCGG_y
Cuts at: 0 746 1531 1574 2776 3257 3615
Size: 746 785 43 1202 481 358
Fragments arranged by size:
1202 785 746 481 358 43
BsrGI T'GTAC_A
Cuts at: 0 1724 2060 3615
Size: 1724 336 1555
Fragments arranged by size:
1724 1555 336
BssHII G'CGCG_C
Cuts at: 0 2299 3615
Size: 2299 1316
BstAPI GCAn_nnn'nTGC
Cuts at: 0 504 1395 3615
Size: 504 891 2220
Fragments arranged by size:
2220 891 504
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1287 1365 2617 3615
Size: 1287 78 1252 998
Fragments arranged by size:
1287 1252 998 78
BstYI r'GATC_y
Cuts at: 0 286 1830 2879 3615
Size: 286 1544 1049 736
Fragments arranged by size:
1544 1049 736 286
Bsu36I CC'TnA_GG
Cuts at: 0 1349 3150 3503 3615
Size: 1349 1801 353 112
Fragments arranged by size:
1801 1349 353 112
BtgI C'CryG_G
Cuts at: 0 37 206 515 641 996 1698 3047 3229
Size: 37 169 309 126 355 702 1349 182
Cuts at: 3229 3584 3615
Size: 355 31
Fragments arranged by size:
1349 702 355 355 309 182 169 126
37 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 831 3615
Size: 831 2784
BtsI GCAGTG_nn'
Cuts at: 0 826 1637 1685 2218 2723 2753 3388 3503
Size: 826 811 48 533 505 30 635 115
Cuts at: 3503 3615
Size: 112
Fragments arranged by size:
826 811 635 533 505 115 112 48
30
DraIII CAC_nnn'GTG
Cuts at: 0 2213 2670 3027 3615
Size: 2213 457 357 588
Fragments arranged by size:
2213 588 457 357
EaeI y'GGCC_r
Cuts at: 0 53 638 875 2637 3071 3615
Size: 53 585 237 1762 434 544
Fragments arranged by size:
1762 585 544 434 237 53
EarI CTCTTCn'nnn_
Cuts at: 0 104 1081 1559 1961 2395 2611 2690 2801
Size: 104 977 478 402 434 216 79 111
Cuts at: 2801 3605 3615
Size: 804 10
Fragments arranged by size:
977 804 478 434 402 216 111 104
79 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 161 3615
Size: 161 3454
EcoICRI GAG'CTC
Cuts at: 0 1074 1935 2700 2868 3414 3615
Size: 1074 861 765 168 546 201
Fragments arranged by size:
1074 861 765 546 201 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 768 1054 1204 1780 2299 2440 2442
Size: 625 143 286 150 576 519 141 2
Cuts at: 2442 2896 3615
Size: 454 719
Fragments arranged by size:
719 625 576 519 454 286 150 143
141 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 508 1825 2290 2317 3615
Size: 508 1317 465 27 1298
Fragments arranged by size:
1317 1298 508 465 27
EcoO109I rG'GnC_Cy
Cuts at: 0 501 855 1140 1257 1344 1353 1602 1957
Size: 501 354 285 117 87 9 249 355
Cuts at: 1957 1958 2514 2765 2841 3239 3240 3615
Size: 1 556 251 76 398 1 375
Fragments arranged by size:
556 501 398 375 355 354 285 251
249 117 87 76 9 1 1
EcoRI G'AATT_C
Cuts at: 0 1405 3615
Size: 1405 2210
EcoRV GAT'ATC
Cuts at: 0 2172 3615
Size: 2172 1443
FspI TGC'GCA
Cuts at: 0 3607 3615
Size: 3607 8
HaeII r_GCGC'y
Cuts at: 0 375 554 981 1010 1100 1306 1581 2356
Size: 375 179 427 29 90 206 275 775
Cuts at: 2356 2364 2409 2964 3615
Size: 8 45 555 651
Fragments arranged by size:
775 651 555 427 375 275 206 179
90 45 29 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 278 310 349 381 844 876
Size: 67 32 179 32 39 32 463 32
Cuts at: 876 931 963 2128 2160 2287 2319 2332 2364
Size: 55 32 1165 32 127 32 13 32
Cuts at: 2364 2871 2903 3580 3612 3615
Size: 507 32 677 32 3
Fragments arranged by size:
1165 677 507 463 179 127 67 55
39 32 32 32 32 32 32 32
32 32 32 13 3
HincII GTy'rAC
Cuts at: 0 2496 2940 3615
Size: 2496 444 675
Fragments arranged by size:
2496 675 444
KpnI G_GTAC'C
Cuts at: 0 2466 3390 3615
Size: 2466 924 225
Fragments arranged by size:
2466 924 225
MfeI C'AATT_G
Cuts at: 0 2585 3615
Size: 2585 1030
MluI A'CGCG_T
Cuts at: 0 3483 3615
Size: 3483 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 2126 3615
Size: 806 1320 1489
Fragments arranged by size:
1489 1320 806
MscI TGG'CCA
Cuts at: 0 55 3073 3615
Size: 55 3018 542
Fragments arranged by size:
3018 542 55
MslI CAynn'nnrTG
Cuts at: 0 125 715 721 1432 2336 2777 3349 3406
Size: 125 590 6 711 904 441 572 57
Cuts at: 3406 3615
Size: 209
Fragments arranged by size:
904 711 590 572 441 209 125 57
6
MspA1I CmG'CkG
Cuts at: 0 1086 1896 1974 2192 2271 2712 3191 3586
Size: 1086 810 78 218 79 441 479 395
Cuts at: 3586 3615
Size: 29
Fragments arranged by size:
1086 810 479 441 395 218 79 78
29
NaeI GCC'GGC
Cuts at: 0 1533 1576 3615
Size: 1533 43 2039
Fragments arranged by size:
2039 1533 43
NcoI C'CATG_G
Cuts at: 0 206 515 1698 3047 3615
Size: 206 309 1183 1349 568
Fragments arranged by size:
1349 1183 568 309 206
NgoMIV G'CCGG_C
Cuts at: 0 1531 1574 3615
Size: 1531 43 2041
Fragments arranged by size:
2041 1531 43
NspI r_CATG'y
Cuts at: 0 2776 3615
Size: 2776 839
PflMI CCAn_nnn'nTGG
Cuts at: 0 2031 2670 3027 3048 3335 3615
Size: 2031 639 357 21 287 280
Fragments arranged by size:
2031 639 357 287 280 21
PfoI T'CCnGG_A
Cuts at: 0 1949 2882 3416 3615
Size: 1949 933 534 199
Fragments arranged by size:
1949 933 534 199
PmlI CAC'GTG
Cuts at: 0 402 2334 3615
Size: 402 1932 1281
Fragments arranged by size:
1932 1281 402
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1897 1929 3615
Size: 1897 32 1686
Fragments arranged by size:
1897 1686 32
PpuMI rG'GwC_Cy
Cuts at: 0 1344 1602 2765 2841 3615
Size: 1344 258 1163 76 774
Fragments arranged by size:
1344 1163 774 258 76
PspOMI G'GGCC_C
Cuts at: 0 536 1556 1898 1957 2596 3239 3615
Size: 536 1020 342 59 639 643 376
Fragments arranged by size:
1020 643 639 536 376 342 59
PstI C_TGCA'G
Cuts at: 0 509 897 1148 1272 1400 1994 3615
Size: 509 388 251 124 128 594 1621
Fragments arranged by size:
1621 594 509 388 251 128 124
PvuI CG_AT'CG
Cuts at: 0 3168 3615
Size: 3168 447
PvuII CAG'CTG
Cuts at: 0 1086 3191 3615
Size: 1086 2105 424
Fragments arranged by size:
2105 1086 424
SacI G_AGCT'C
Cuts at: 0 1076 1937 2702 2870 3416 3615
Size: 1076 861 765 168 546 199
Fragments arranged by size:
1076 861 765 546 199 168
SacII CC_GC'GG
Cuts at: 0 3587 3615
Size: 3587 28
SanDI GG'GwC_CC
Cuts at: 0 1344 2765 3615
Size: 1344 1421 850
Fragments arranged by size:
1421 1344 850
SapI GCTCTTCn'nnn_
Cuts at: 0 1081 1961 2395 2611 2690 3615
Size: 1081 880 434 216 79 925
Fragments arranged by size:
1081 925 880 434 216 79
SexAI A'CCwGG_T
Cuts at: 0 2024 3023 3615
Size: 2024 999 592
Fragments arranged by size:
2024 999 592
SfcI C'TryA_G
Cuts at: 0 505 893 1144 1268 1396 1543 1891 1990
Size: 505 388 251 124 128 147 348 99
Cuts at: 1990 3615
Size: 1625
Fragments arranged by size:
1625 505 388 348 251 147 128 124
99
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3052 3615
Size: 3052 563
SmaI CCC'GGG
Cuts at: 0 535 1561 1843 3493 3615
Size: 535 1026 282 1650 122
Fragments arranged by size:
1650 1026 535 282 122
SmlI C'TyrA_G
Cuts at: 0 2314 2356 3615
Size: 2314 42 1259
Fragments arranged by size:
2314 1259 42
SphI G_CATG'C
Cuts at: 0 2776 3615
Size: 2776 839
SrfI GCCC'GGGC
Cuts at: 0 535 3615
Size: 535 3080
StuI AGG'CCT
Cuts at: 0 2425 3615
Size: 2425 1190
StyI C'CwwG_G
Cuts at: 0 96 206 515 1019 1590 1698 3047 3615
Size: 96 110 309 504 571 108 1349 568
Fragments arranged by size:
1349 571 568 504 309 110 108 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 300 1711 3342 3615
Size: 300 1411 1631 273
Fragments arranged by size:
1631 1411 300 273
TatI w'GTAC_w
Cuts at: 0 1724 2060 2531 3615
Size: 1724 336 471 1084
Fragments arranged by size:
1724 1084 471 336
Tth111I GACn'n_nGTC
Cuts at: 0 1600 3615
Size: 1600 2015
XbaI T'CTAG_A
Cuts at: 0 675 3615
Size: 675 2940
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 694 1228 1284 3332 3615
Size: 694 534 56 2048 283
Fragments arranged by size:
2048 694 534 283 56
XmaI C'CCGG_G
Cuts at: 0 533 1559 1841 3491 3615
Size: 533 1026 282 1650 124
Fragments arranged by size:
1650 1026 533 282 124
XmnI GAAnn'nnTTC
Cuts at: 0 1108 1409 3615
Size: 1108 301 2206
Fragments arranged by size:
2206 1108 301
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BaeI
BanI BanII BbsI BbvCI BciVI BclI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PflMI PfoI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII SacI SacII SanDI SapI
SexAI SfcI SfiI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AscI AseI AsiSI AvrII
BamHI BbeI BcgI BfrBI BglII BmgBI BmtI BsiWI
BsrDI BssSI BstBI BstEII BstZ17I ClaI CspCI DraI
DrdI EagI FalI FseI FspAI HindIII HpaI KasI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PshAI PsiI PspXI PsrI RsrII SalI
SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI SspI
SwaI TstI XhoI ZraI