Restriction map for
pF1KA0783
(Linear) (Six-base) MAPSORT of: KIAA0783.seq Check: 7875 from: 1 to: 2664
KIAA0783.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 15 2664
Size: 15 2649
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 203 1606 1703 2612 2664
Size: 137 66 1403 97 909 52
Fragments arranged by size:
1403 909 137 97 66 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1401 1649 2664
Size: 1401 248 1015
Fragments arranged by size:
1401 1015 248
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1193 1225 2664
Size: 1193 32 1439
Fragments arranged by size:
1439 1193 32
AlwNI CAG_nnn'CTG
Cuts at: 0 115 421 1093 1198 2664
Size: 115 306 672 105 1466
Fragments arranged by size:
1466 672 306 115 105
ApoI r'AATT_y
Cuts at: 0 246 1178 1895 2425 2664
Size: 246 932 717 530 239
Fragments arranged by size:
932 717 530 246 239
AseI AT'TA_AT
Cuts at: 0 286 2664
Size: 286 2378
BamHI G'GATC_C
Cuts at: 0 2265 2664
Size: 2265 399
BanI G'GyrC_C
Cuts at: 0 1309 2664
Size: 1309 1355
BanII G_rGCy'C
Cuts at: 0 937 2664
Size: 937 1727
BbsI GAAGACnn'nnnn_
Cuts at: 0 749 1340 2559 2664
Size: 749 591 1219 105
Fragments arranged by size:
1219 749 591 105
BclI T'GATC_A
Cuts at: 0 2205 2664
Size: 2205 459
BglII A'GATC_T
Cuts at: 0 670 2664
Size: 670 1994
Bme1580I G_kGCm'C
Cuts at: 0 1227 2664
Size: 1227 1437
BmrI ACTGGGnnnn_n'
Cuts at: 0 1364 2664
Size: 1364 1300
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1089 2664
Size: 1089 1575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 951 1259 2651 2664
Size: 951 308 1392 13
Fragments arranged by size:
1392 951 308 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 2664
Size: 934 1730
BsaBI GATnn'nnATC
Cuts at: 0 675 770 2210 2664
Size: 675 95 1440 454
Fragments arranged by size:
1440 675 454 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 453 483 2664
Size: 453 30 2181
Fragments arranged by size:
2181 453 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 2261 2664
Size: 33 2228 403
Fragments arranged by size:
2228 403 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1407 2664
Size: 1407 1257
BsiHKAI G_wGCw'C
Cuts at: 0 937 1416 2106 2320 2664
Size: 937 479 690 214 344
Fragments arranged by size:
937 690 479 344 214
Bsp1286I G_dGCh'C
Cuts at: 0 937 1227 1416 2106 2320 2664
Size: 937 290 189 690 214 344
Fragments arranged by size:
937 690 344 290 214 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 15 2664
Size: 15 2649
BsrDI GCAATG_nn'
Cuts at: 0 768 2664
Size: 768 1896
BsrFI r'CCGG_y
Cuts at: 0 2088 2664
Size: 2088 576
BssSI C'ACGA_G
Cuts at: 0 2478 2664
Size: 2478 186
BstBI TT'CG_AA
Cuts at: 0 2025 2664
Size: 2025 639
BstXI CCAn_nnnn'nTGG
Cuts at: 0 545 2664
Size: 545 2119
BstYI r'GATC_y
Cuts at: 0 670 1464 2265 2664
Size: 670 794 801 399
Fragments arranged by size:
801 794 670 399
Bsu36I CC'TnA_GG
Cuts at: 0 2513 2664
Size: 2513 151
BtgI C'CryG_G
Cuts at: 0 587 2366 2664
Size: 587 1779 298
Fragments arranged by size:
1779 587 298
BtsI GCAGTG_nn'
Cuts at: 0 1719 1844 2320 2349 2664
Size: 1719 125 476 29 315
Fragments arranged by size:
1719 476 315 125 29
DraI TTT'AAA
Cuts at: 0 93 2664
Size: 93 2571
EaeI y'GGCC_r
Cuts at: 0 2363 2664
Size: 2363 301
EarI CTCTTCn'nnn_
Cuts at: 0 587 808 913 1445 1513 2165 2664
Size: 587 221 105 532 68 652 499
Fragments arranged by size:
652 587 532 499 221 105 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1462 2664
Size: 1462 1202
EcoICRI GAG'CTC
Cuts at: 0 935 2664
Size: 935 1729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 203 951 1259 1606 1703 2612 2651
Size: 137 66 748 308 347 97 909 39
Cuts at: 2651 2664
Size: 13
Fragments arranged by size:
909 748 347 308 137 97 66 39
13
EcoRV GAT'ATC
Cuts at: 0 1773 2664
Size: 1773 891
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 256 1447 1975 2664
Size: 256 1191 528 689
Fragments arranged by size:
1191 689 528 256
FspI TGC'GCA
Cuts at: 0 1885 2664
Size: 1885 779
FspAI rTGC'GCAy
Cuts at: 0 1885 2664
Size: 1885 779
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 568 600 864 896 1398 1430 2142
Size: 27 541 32 264 32 502 32 712
Cuts at: 2142 2174 2664
Size: 32 490
Fragments arranged by size:
712 541 502 490 264 32 32 32
32 27
HincII GTy'rAC
Cuts at: 0 480 2664
Size: 480 2184
HindIII A'AGCT_T
Cuts at: 0 1957 2502 2664
Size: 1957 545 162
Fragments arranged by size:
1957 545 162
HpaI GTT'AAC
Cuts at: 0 480 2664
Size: 480 2184
MfeI C'AATT_G
Cuts at: 0 1020 2664
Size: 1020 1644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 2664
Size: 78 2586
MscI TGG'CCA
Cuts at: 0 2365 2664
Size: 2365 299
MspA1I CmG'CkG
Cuts at: 0 1444 2664
Size: 1444 1220
NcoI C'CATG_G
Cuts at: 0 587 2366 2664
Size: 587 1779 298
Fragments arranged by size:
1779 587 298
NspI r_CATG'y
Cuts at: 0 1495 2664
Size: 1495 1169
PacI TTA_AT'TAA
Cuts at: 0 290 2664
Size: 290 2374
PfoI T'CCnGG_A
Cuts at: 0 1236 2664
Size: 1236 1428
PstI C_TGCA'G
Cuts at: 0 255 1851 2169 2664
Size: 255 1596 318 495
Fragments arranged by size:
1596 495 318 255
SacI G_AGCT'C
Cuts at: 0 937 2664
Size: 937 1727
SapI GCTCTTCn'nnn_
Cuts at: 0 587 2664
Size: 587 2077
ScaI AGT'ACT
Cuts at: 0 266 2171 2664
Size: 266 1905 493
Fragments arranged by size:
1905 493 266
SfcI C'TryA_G
Cuts at: 0 171 251 515 650 1540 1847 2165 2664
Size: 171 80 264 135 890 307 318 499
Fragments arranged by size:
890 499 318 307 264 171 135 80
SmlI C'TyrA_G
Cuts at: 0 913 2664
Size: 913 1751
SpeI A'CTAG_T
Cuts at: 0 1798 2664
Size: 1798 866
SspI AAT'ATT
Cuts at: 0 192 2664
Size: 192 2472
StuI AGG'CCT
Cuts at: 0 1672 2664
Size: 1672 992
StyI C'CwwG_G
Cuts at: 0 587 1111 1313 1681 2108 2366 2585 2664
Size: 587 524 202 368 427 258 219 79
Fragments arranged by size:
587 524 427 368 258 219 202 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2121 2664
Size: 2121 543
TatI w'GTAC_w
Cuts at: 0 264 1765 2169 2664
Size: 264 1501 404 495
Fragments arranged by size:
1501 495 404 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 453 485 2664
Size: 453 32 2179
Fragments arranged by size:
2179 453 32
XmnI GAAnn'nnTTC
Cuts at: 0 1007 1958 2537 2664
Size: 1007 951 579 127
Fragments arranged by size:
1007 951 579 127
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApoI AseI BamHI
BanI BanII BbsI BclI BglII Bme1580I BmrI BplI
BpmI BpuEI BsaBI BsaXI BseRI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BssSI BstBI BstXI BstYI Bsu36I
BtgI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI
EcoRV FalI FspI FspAI Hin4I HincII HindIII HpaI
MfeI MmeI MscI MspA1I NcoI NspI PacI PfoI
PstI SacI SapI ScaI SfcI SmlI SpeI SspI
StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BbeI BbvCI BcgI BciVI BfrBI BglI BlpI
BmgBI BmtI Bpu10I BsaI BsaAI BsaHI BsaWI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BstAPI BstEII BstZ17I BtgZI ClaI CspCI DraIII
DrdI EagI EcoNI EcoO109I EcoRI FseI HaeII KasI
KpnI MluI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI