Restriction map for
pF1KSDA0792
(Linear) (Six-base) MAPSORT of: KIAA0792.seq Check: 1717 from: 1 to: 2421
KIAA0792.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 858 2152 2421
Size: 858 1294 269
Fragments arranged by size:
1294 858 269
AatII G_ACGT'C
Cuts at: 0 173 2421
Size: 173 2248
AccI GT'mk_AC
Cuts at: 0 1448 1517 1958 2421
Size: 1448 69 441 463
Fragments arranged by size:
1448 463 441 69
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 2421
Size: 1108 1313
AflIII A'CryG_T
Cuts at: 0 749 760 2421
Size: 749 11 1661
Fragments arranged by size:
1661 749 11
AhdI GACnn_n'nnGTC
Cuts at: 0 267 2421
Size: 267 2154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1729 2421
Size: 1729 692
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 30 1007 1039 2421
Size: 30 977 32 1382
Fragments arranged by size:
1382 977 32 30
AlwNI CAG_nnn'CTG
Cuts at: 0 384 1038 1266 2421
Size: 384 654 228 1155
Fragments arranged by size:
1155 654 384 228
AvaI C'yCGr_G
Cuts at: 0 67 2421
Size: 67 2354
BamHI G'GATC_C
Cuts at: 0 1985 2421
Size: 1985 436
BanI G'GyrC_C
Cuts at: 0 635 1657 2332 2421
Size: 635 1022 675 89
Fragments arranged by size:
1022 675 635 89
BanII G_rGCy'C
Cuts at: 0 1142 1169 2421
Size: 1142 27 1252
Fragments arranged by size:
1252 1142 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 838 880 959 1632 1842 2421
Size: 838 42 79 673 210 579
Fragments arranged by size:
838 673 579 210 79 42
BbvCI CC'TCA_GC
Cuts at: 0 2167 2421
Size: 2167 254
BciVI GTATCCnnnnn_n'
Cuts at: 0 764 2421
Size: 764 1657
BclI T'GATC_A
Cuts at: 0 1754 2421
Size: 1754 667
BfrBI ATG'CAT
Cuts at: 0 1834 2421
Size: 1834 587
BglI GCCn_nnn'nGGC
Cuts at: 0 1768 2421
Size: 1768 653
BmgBI CAC'GTC
Cuts at: 0 1023 2421
Size: 1023 1398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 109 1015 1486 1996 2421
Size: 40 69 906 471 510 425
Fragments arranged by size:
906 510 471 425 69 40
Bpu10I CC'TnA_GC
Cuts at: 0 2167 2421
Size: 2167 254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1906 2421
Size: 1906 515
BsaI GGTCTCn'nnnn_
Cuts at: 0 1567 1735 2421
Size: 1567 168 686
Fragments arranged by size:
1567 686 168
BsaAI yAC'GTr
Cuts at: 0 761 2256 2421
Size: 761 1495 165
Fragments arranged by size:
1495 761 165
BsaBI GATnn'nnATC
Cuts at: 0 288 2421
Size: 288 2133
BsaHI Gr'CG_yC
Cuts at: 0 170 404 956 2421
Size: 170 234 552 1465
Fragments arranged by size:
1465 552 234 170
BsaWI w'CCGG_w
Cuts at: 0 1586 2421
Size: 1586 835
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 291 679 2421
Size: 291 388 1742
Fragments arranged by size:
1742 388 291
BseYI C'CCAG_C
Cuts at: 0 60 330 936 1142 1266 1965 2421
Size: 60 270 606 206 124 699 456
Fragments arranged by size:
699 606 456 270 206 124 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 799 2421
Size: 568 231 1622
Fragments arranged by size:
1622 568 231
BsiHKAI G_wGCw'C
Cuts at: 0 125 1169 2421
Size: 125 1044 1252
Fragments arranged by size:
1252 1044 125
Bsp1286I G_dGCh'C
Cuts at: 0 125 1142 1169 2421
Size: 125 1017 27 1252
Fragments arranged by size:
1252 1017 125 27
BspHI T'CATG_A
Cuts at: 0 1502 2421
Size: 1502 919
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 858 872 2152 2421
Size: 858 14 1280 269
Fragments arranged by size:
1280 858 269 14
BsrDI GCAATG_nn'
Cuts at: 0 239 1707 2421
Size: 239 1468 714
Fragments arranged by size:
1468 714 239
BsrFI r'CCGG_y
Cuts at: 0 74 1937 2421
Size: 74 1863 484
Fragments arranged by size:
1863 484 74
BstEII G'GTnAC_C
Cuts at: 0 132 2421
Size: 132 2289
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1629 2421
Size: 1629 792
BstYI r'GATC_y
Cuts at: 0 380 1985 2421
Size: 380 1605 436
Fragments arranged by size:
1605 436 380
Bsu36I CC'TnA_GG
Cuts at: 0 303 1208 2421
Size: 303 905 1213
Fragments arranged by size:
1213 905 303
BtgI C'CryG_G
Cuts at: 0 1073 1334 2387 2421
Size: 1073 261 1053 34
Fragments arranged by size:
1073 1053 261 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1665 1875 2421
Size: 1665 210 546
Fragments arranged by size:
1665 546 210
BtsI GCAGTG_nn'
Cuts at: 0 47 1459 2400 2421
Size: 47 1412 941 21
Fragments arranged by size:
1412 941 47 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 891 926 2421
Size: 891 35 1495
Fragments arranged by size:
1495 891 35
DraIII CAC_nnn'GTG
Cuts at: 0 1932 2421
Size: 1932 489
DrdI GACnn_nn'nnGTC
Cuts at: 0 2298 2421
Size: 2298 123
EaeI y'GGCC_r
Cuts at: 0 104 234 797 912 1076 1760 2421
Size: 104 130 563 115 164 684 661
Fragments arranged by size:
684 661 563 164 130 115 104
EarI CTCTTCn'nnn_
Cuts at: 0 616 835 1291 2041 2186 2421
Size: 616 219 456 750 145 235
Fragments arranged by size:
750 616 456 235 219 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 696 2421
Size: 696 1725
EcoICRI GAG'CTC
Cuts at: 0 1167 2421
Size: 1167 1254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 109 1015 1108 1486 1996 2421
Size: 40 69 906 93 378 510 425
Fragments arranged by size:
906 510 425 378 93 69 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 426 817 1623 2286 2421
Size: 426 391 806 663 135
Fragments arranged by size:
806 663 426 391 135
EcoO109I rG'GnC_Cy
Cuts at: 0 1242 2079 2421
Size: 1242 837 342
Fragments arranged by size:
1242 837 342
HaeII r_GCGC'y
Cuts at: 0 2387 2421
Size: 2387 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 253 285 946 978 2421
Size: 253 32 661 32 1443
Fragments arranged by size:
1443 661 253 32 32
HincII GTy'rAC
Cuts at: 0 310 492 2421
Size: 310 182 1929
Fragments arranged by size:
1929 310 182
MluI A'CGCG_T
Cuts at: 0 749 2421
Size: 749 1672
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1610 2421
Size: 1610 811
MscI TGG'CCA
Cuts at: 0 799 914 1078 1762 2421
Size: 799 115 164 684 659
Fragments arranged by size:
799 684 659 164 115
MslI CAynn'nnrTG
Cuts at: 0 976 1756 2421
Size: 976 780 665
Fragments arranged by size:
976 780 665
MspA1I CmG'CkG
Cuts at: 0 60 176 783 885 1250 1999 2351 2421
Size: 60 116 607 102 365 749 352 70
Fragments arranged by size:
749 607 365 352 116 102 70 60
NcoI C'CATG_G
Cuts at: 0 1073 1334 2421
Size: 1073 261 1087
Fragments arranged by size:
1087 1073 261
NsiI A_TGCA'T
Cuts at: 0 1836 2421
Size: 1836 585
NspI r_CATG'y
Cuts at: 0 2235 2421
Size: 2235 186
PasI CC'CwG_GG
Cuts at: 0 1038 1557 2421
Size: 1038 519 864
Fragments arranged by size:
1038 864 519
PflMI CCAn_nnn'nTGG
Cuts at: 0 384 1038 2013 2421
Size: 384 654 975 408
Fragments arranged by size:
975 654 408 384
PfoI T'CCnGG_A
Cuts at: 0 17 383 743 1025 1061 2421
Size: 17 366 360 282 36 1360
Fragments arranged by size:
1360 366 360 282 36 17
PmlI CAC'GTG
Cuts at: 0 761 2421
Size: 761 1660
PshAI GACnn'nnGTC
Cuts at: 0 1513 2421
Size: 1513 908
PsiI TTA'TAA
Cuts at: 0 211 2421
Size: 211 2210
PstI C_TGCA'G
Cuts at: 0 869 2421
Size: 869 1552
PvuII CAG'CTG
Cuts at: 0 60 176 783 2421
Size: 60 116 607 1638
Fragments arranged by size:
1638 607 116 60
SacI G_AGCT'C
Cuts at: 0 1169 2421
Size: 1169 1252
SapI GCTCTTCn'nnn_
Cuts at: 0 835 2186 2421
Size: 835 1351 235
Fragments arranged by size:
1351 835 235
SbfI CC_TGCA'GG
Cuts at: 0 869 2421
Size: 869 1552
SexAI A'CCwGG_T
Cuts at: 0 671 2421
Size: 671 1750
SfcI C'TryA_G
Cuts at: 0 556 865 1258 1869 2421
Size: 556 309 393 611 552
Fragments arranged by size:
611 556 552 393 309
SmlI C'TyrA_G
Cuts at: 0 1921 2421
Size: 1921 500
StuI AGG'CCT
Cuts at: 0 1618 1804 2011 2421
Size: 1618 186 207 410
Fragments arranged by size:
1618 410 207 186
StyI C'CwwG_G
Cuts at: 0 35 1073 1334 2012 2421
Size: 35 1038 261 678 409
Fragments arranged by size:
1038 678 409 261 35
TatI w'GTAC_w
Cuts at: 0 656 2421
Size: 656 1765
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 403 435 631 663 1830 1862 2421
Size: 403 32 196 32 1167 32 559
Fragments arranged by size:
1167 559 403 196 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1482 2421
Size: 1482 939
XbaI T'CTAG_A
Cuts at: 0 1572 2421
Size: 1572 849
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 765 1816 2394 2421
Size: 765 1051 578 27
Fragments arranged by size:
1051 765 578 27
XmnI GAAnn'nnTTC
Cuts at: 0 1096 2421
Size: 1096 1325
ZraI GAC'GTC
Cuts at: 0 171 2421
Size: 171 2250
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AlfI AloI
AlwNI AvaI BamHI BanI BanII BbsI BbvCI BciVI
BclI BfrBI BglI BmgBI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BsrFI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII MluI MmeI MscI MslI MspA1I NcoI NsiI
NspI PasI PflMI PfoI PmlI PshAI PsiI PstI
PvuII SacI SapI SbfI SexAI SfcI SmlI StuI
StyI TatI TstI Tth111I XbaI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AleI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BglII BlpI Bme1580I BmrI BmtI BplI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I ClaI DraI EagI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PciI PmeI PpiI PpuMI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII XhoI XmaI