Restriction map for
pF1KSDA0797
(Linear) (Six-base) MAPSORT of: KIAA0797.seq Check: 7830 from: 1 to: 3336
KIAA0797.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 402 739 1347 2792 3336
Size: 402 337 608 1445 544
Fragments arranged by size:
1445 608 544 402 337
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2116 2402 2780 3063 3247 3336
Size: 2116 286 378 283 184 89
Fragments arranged by size:
2116 378 286 283 184 89
AflII C'TTAA_G
Cuts at: 0 2721 3336
Size: 2721 615
AflIII A'CryG_T
Cuts at: 0 326 3336
Size: 326 3010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1576 3336
Size: 1576 1760
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 377 409 3336
Size: 377 32 2927
Fragments arranged by size:
2927 377 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3086 3336
Size: 3086 250
ApoI r'AATT_y
Cuts at: 0 247 774 1620 1745 1888 2038 2117 2617
Size: 247 527 846 125 143 150 79 500
Cuts at: 2617 2966 3159 3336
Size: 349 193 177
Fragments arranged by size:
846 527 500 349 247 193 177 150
143 125 79
AseI AT'TA_AT
Cuts at: 0 515 1700 3336
Size: 515 1185 1636
Fragments arranged by size:
1636 1185 515
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 618 651 3336
Size: 618 33 2685
Fragments arranged by size:
2685 618 33
BanII G_rGCy'C
Cuts at: 0 3049 3336
Size: 3049 287
BbsI GAAGACnn'nnnn_
Cuts at: 0 265 1175 2111 3336
Size: 265 910 936 1225
Fragments arranged by size:
1225 936 910 265
BbvCI CC'TCA_GC
Cuts at: 0 1058 3336
Size: 1058 2278
BciVI GTATCCnnnnn_n'
Cuts at: 0 2926 3336
Size: 2926 410
BclI T'GATC_A
Cuts at: 0 108 3336
Size: 108 3228
BlpI GC'TnA_GC
Cuts at: 0 1549 3336
Size: 1549 1787
BmrI ACTGGGnnnn_n'
Cuts at: 0 1942 3336
Size: 1942 1394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 800 1268 1940 1964 3336
Size: 800 468 672 24 1372
Fragments arranged by size:
1372 800 672 468 24
Bpu10I CC'TnA_GC
Cuts at: 0 1058 1985 3336
Size: 1058 927 1351
Fragments arranged by size:
1351 1058 927
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1259 3336
Size: 1259 2077
BsaAI yAC'GTr
Cuts at: 0 2241 3336
Size: 2241 1095
BsaBI GATnn'nnATC
Cuts at: 0 2516 3336
Size: 2516 820
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 641 644 1046 2181 3336
Size: 641 3 402 1135 1155
Fragments arranged by size:
1155 1135 641 402 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1073 2002 2273 3336
Size: 1073 929 271 1063
Fragments arranged by size:
1073 1063 929 271
BsiEI CG_ry'CG
Cuts at: 0 8 3336
Size: 8 3328
BsmI GAATG_Cn'
Cuts at: 0 1160 3336
Size: 1160 2176
Bsp1286I G_dGCh'C
Cuts at: 0 3049 3336
Size: 3049 287
BspHI T'CATG_A
Cuts at: 0 111 2175 3336
Size: 111 2064 1161
Fragments arranged by size:
2064 1161 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2024 3336
Size: 2024 1312
BsrBI CCG'CTC
Cuts at: 0 17 3336
Size: 17 3319
BsrDI GCAATG_nn'
Cuts at: 0 1192 2493 3336
Size: 1192 1301 843
Fragments arranged by size:
1301 1192 843
BsrFI r'CCGG_y
Cuts at: 0 7 3336
Size: 7 3329
BsrGI T'GTAC_A
Cuts at: 0 1783 3336
Size: 1783 1553
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2430 3336
Size: 2430 906
BstZ17I GTA'TAC
Cuts at: 0 1348 3336
Size: 1348 1988
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2762 3336
Size: 2762 574
BtsI GCAGTG_nn'
Cuts at: 0 2571 2835 3336
Size: 2571 264 501
Fragments arranged by size:
2571 501 264
DraI TTT'AAA
Cuts at: 0 90 294 1334 2045 2888 3336
Size: 90 204 1040 711 843 448
Fragments arranged by size:
1040 843 711 448 204 90
EaeI y'GGCC_r
Cuts at: 0 5 3336
Size: 5 3331
EagI C'GGCC_G
Cuts at: 0 5 3336
Size: 5 3331
EarI CTCTTCn'nnn_
Cuts at: 0 566 1035 1369 1793 2457 3336
Size: 566 469 334 424 664 879
Fragments arranged by size:
879 664 566 469 424 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 800 1268 1940 1964 2116 2402 2780 3063
Size: 800 468 672 24 152 286 378 283
Cuts at: 3063 3247 3336
Size: 184 89
Fragments arranged by size:
800 672 468 378 286 283 184 152
89 24
EcoO109I rG'GnC_Cy
Cuts at: 0 2013 2941 3336
Size: 2013 928 395
Fragments arranged by size:
2013 928 395
EcoRI G'AATT_C
Cuts at: 0 247 774 2117 2617 3336
Size: 247 527 1343 500 719
Fragments arranged by size:
1343 719 527 500 247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 93 125 565 597 1953 1985 2157 2189
Size: 93 32 440 32 1356 32 172 32
Cuts at: 2189 3194 3226 3262 3294 3336
Size: 1005 32 36 32 42
Fragments arranged by size:
1356 1005 440 172 93 42 36 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2793 3336
Size: 2793 543
HindIII A'AGCT_T
Cuts at: 0 50 3336
Size: 50 3286
MfeI C'AATT_G
Cuts at: 0 314 851 3336
Size: 314 537 2485
Fragments arranged by size:
2485 537 314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1581 3336
Size: 1581 1755
MslI CAynn'nnrTG
Cuts at: 0 419 2360 2366 2714 3336
Size: 419 1941 6 348 622
Fragments arranged by size:
1941 622 419 348 6
NaeI GCC'GGC
Cuts at: 0 9 3336
Size: 9 3327
NdeI CA'TA_TG
Cuts at: 0 264 2560 3336
Size: 264 2296 776
Fragments arranged by size:
2296 776 264
NgoMIV G'CCGG_C
Cuts at: 0 7 3336
Size: 7 3329
NspI r_CATG'y
Cuts at: 0 330 1079 3336
Size: 330 749 2257
Fragments arranged by size:
2257 749 330
PciI A'CATG_T
Cuts at: 0 326 3336
Size: 326 3010
PflMI CCAn_nnn'nTGG
Cuts at: 0 1099 3336
Size: 1099 2237
PpuMI rG'GwC_Cy
Cuts at: 0 2013 3336
Size: 2013 1323
PshAI GACnn'nnGTC
Cuts at: 0 789 3336
Size: 789 2547
PsiI TTA'TAA
Cuts at: 0 2549 3336
Size: 2549 787
PstI C_TGCA'G
Cuts at: 0 2863 3336
Size: 2863 473
SalI G'TCGA_C
Cuts at: 0 2791 3336
Size: 2791 545
SfcI C'TryA_G
Cuts at: 0 389 630 1442 1508 2393 2680 2742 2859
Size: 389 241 812 66 885 287 62 117
Cuts at: 2859 3232 3336
Size: 373 104
Fragments arranged by size:
885 812 389 373 287 241 117 104
66 62
SmlI C'TyrA_G
Cuts at: 0 1274 2721 3336
Size: 1274 1447 615
Fragments arranged by size:
1447 1274 615
StyI C'CwwG_G
Cuts at: 0 540 3336
Size: 540 2796
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 482 2129 2939 3336
Size: 482 1647 810 397
Fragments arranged by size:
1647 810 482 397
TatI w'GTAC_w
Cuts at: 0 612 1783 3336
Size: 612 1171 1553
Fragments arranged by size:
1553 1171 612
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 482 514 2652 2684 3336
Size: 482 32 2138 32 652
Fragments arranged by size:
2138 652 482 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1324 3336
Size: 1324 2012
XmnI GAAnn'nnTTC
Cuts at: 0 845 3024 3336
Size: 845 2179 312
Fragments arranged by size:
2179 845 312
Enzymes that do cut:
AccI AcuI AflII AflIII AlfI AloI AlwNI ApoI
AseI BaeI BanII BbsI BbvCI BciVI BclI BlpI
BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BseRI BsgI
BsiEI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstXI BstZ17I BtgZI BtsI DraI EaeI EagI
EarI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII MfeI
MmeI MslI NaeI NdeI NgoMIV NspI PciI PflMI
PpuMI PshAI PsiI PstI SalI SfcI SmlI StyI
TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
ApaI ApaLI AscI AsiSI AvaI AvrII BamHI BanI
BbeI BcgI BfrBI BglI BglII Bme1580I BmgBI BmtI
BplI BsaI BsaHI BsaWI BsaXI BseYI BsiHKAI BsiWI
BsmBI BspEI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI ClaI CspCI DraIII DrdI EciI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HpaI
KasI KpnI MluI MscI MspA1I NarI NcoI NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XcmI XhoI XmaI ZraI