Restriction map for
pF1KSDA0798
(Linear) (Six-base) MAPSORT of: KIAA0798.seq Check: 5398 from: 1 to: 1956
KIAA0798.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1956
Size: 313 1643
AlwNI CAG_nnn'CTG
Cuts at: 0 72 1956
Size: 72 1884
ApaI G_GGCC'C
Cuts at: 0 79 1956
Size: 79 1877
ApoI r'AATT_y
Cuts at: 0 417 469 532 758 1682 1956
Size: 417 52 63 226 924 274
Fragments arranged by size:
924 417 274 226 63 52
BanII G_rGCy'C
Cuts at: 0 79 1956
Size: 79 1877
BbsI GAAGACnn'nnnn_
Cuts at: 0 798 1956
Size: 798 1158
BclI T'GATC_A
Cuts at: 0 2 255 663 1956
Size: 2 253 408 1293
Fragments arranged by size:
1293 408 253 2
BfrBI ATG'CAT
Cuts at: 0 325 1956
Size: 325 1631
BglII A'GATC_T
Cuts at: 0 116 1956
Size: 116 1840
Bme1580I G_kGCm'C
Cuts at: 0 79 1956
Size: 79 1877
BmrI ACTGGGnnnn_n'
Cuts at: 0 989 1956
Size: 989 967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 711 872 956 1460 1544 1628 1796
Size: 49 662 161 84 504 84 84 168
Cuts at: 1796 1956
Size: 160
Fragments arranged by size:
662 504 168 161 160 84 84 84
49
BsaAI yAC'GTr
Cuts at: 0 615 1956
Size: 615 1341
BsaBI GATnn'nnATC
Cuts at: 0 257 1956
Size: 257 1699
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 200 1893 1956
Size: 73 127 1693 63
Fragments arranged by size:
1693 127 73 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1479 1647 1956
Size: 1479 168 309
Fragments arranged by size:
1479 309 168
Bsp1286I G_dGCh'C
Cuts at: 0 79 1956
Size: 79 1877
BspHI T'CATG_A
Cuts at: 0 378 594 666 1956
Size: 378 216 72 1290
Fragments arranged by size:
1290 378 216 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 137 1956
Size: 137 1819
BsrDI GCAATG_nn'
Cuts at: 0 879 1131 1551 1956
Size: 879 252 420 405
Fragments arranged by size:
879 420 405 252
BsrGI T'GTAC_A
Cuts at: 0 915 1251 1419 1587 1671 1923 1956
Size: 915 336 168 168 84 252 33
Fragments arranged by size:
915 336 252 168 168 84 33
BstYI r'GATC_y
Cuts at: 0 116 1956
Size: 116 1840
BtgI C'CryG_G
Cuts at: 0 196 1956
Size: 196 1760
BtsI GCAGTG_nn'
Cuts at: 0 627 795 1215 1383 1467 1635 1887 1956
Size: 627 168 420 168 84 168 252 69
Fragments arranged by size:
627 420 252 168 168 168 84 69
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 861 896 1113 1148 1533 1568 1956
Size: 861 35 217 35 385 35 388
Fragments arranged by size:
861 388 385 217 35 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 289 811 979 1147 1231 1399 1688 1719
Size: 289 522 168 168 84 168 289 31
Cuts at: 1719 1819 1956
Size: 100 137
Fragments arranged by size:
522 289 289 168 168 168 137 100
84 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 313 711 872 956 1460 1544 1628
Size: 49 264 398 161 84 504 84 84
Cuts at: 1628 1796 1956
Size: 168 160
Fragments arranged by size:
504 398 264 168 161 160 84 84
84 49
EcoO109I rG'GnC_Cy
Cuts at: 0 76 1956
Size: 76 1880
EcoRI G'AATT_C
Cuts at: 0 758 1956
Size: 758 1198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 154 186 472 504 819 851 1155 1187
Size: 154 32 286 32 315 32 304 32
Cuts at: 1187 1407 1439 1491 1523 1956
Size: 220 32 52 32 433
Fragments arranged by size:
433 315 304 286 220 154 52 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 432 1956
Size: 432 1524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 158 1956
Size: 92 66 1798
Fragments arranged by size:
1798 92 66
MslI CAynn'nnrTG
Cuts at: 0 1119 1287 1956
Size: 1119 168 669
Fragments arranged by size:
1119 669 168
NcoI C'CATG_G
Cuts at: 0 196 1956
Size: 196 1760
NdeI CA'TA_TG
Cuts at: 0 868 1204 1540 1956
Size: 868 336 336 416
Fragments arranged by size:
868 416 336 336
NsiI A_TGCA'T
Cuts at: 0 327 1956
Size: 327 1629
NspI r_CATG'y
Cuts at: 0 993 1329 1376 1956
Size: 993 336 47 580
Fragments arranged by size:
993 580 336 47
PspOMI G'GGCC_C
Cuts at: 0 75 1956
Size: 75 1881
PstI C_TGCA'G
Cuts at: 0 266 528 1956
Size: 266 262 1428
Fragments arranged by size:
1428 266 262
SapI GCTCTTCn'nnn_
Cuts at: 0 811 979 1147 1819 1956
Size: 811 168 168 672 137
Fragments arranged by size:
811 672 168 168 137
SfcI C'TryA_G
Cuts at: 0 120 262 524 1038 1956
Size: 120 142 262 514 918
Fragments arranged by size:
918 514 262 142 120
SphI G_CATG'C
Cuts at: 0 993 1329 1956
Size: 993 336 627
Fragments arranged by size:
993 627 336
StyI C'CwwG_G
Cuts at: 0 196 1736 1956
Size: 196 1540 220
Fragments arranged by size:
1540 220 196
TatI w'GTAC_w
Cuts at: 0 544 703 915 1251 1419 1587 1671 1923
Size: 544 159 212 336 168 168 84 252
Cuts at: 1923 1956
Size: 33
Fragments arranged by size:
544 336 252 212 168 168 159 84
33
XmnI GAAnn'nnTTC
Cuts at: 0 1057 1141 1393 1477 1956
Size: 1057 84 252 84 479
Fragments arranged by size:
1057 479 252 84 84
Enzymes that do cut:
AcuI AlwNI ApaI ApoI BanII BbsI BclI BfrBI
BglII Bme1580I BmrI BpmI BsaAI BsaBI BseRI BsgI
Bsp1286I BspHI BspMI BsrDI BsrGI BstYI BtgI BtsI
CspCI EarI Eco57MI EcoO109I EcoRI Hin4I HincII MmeI
MslI NcoI NdeI NsiI NspI PspOMI PstI SapI
SfcI SphI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BglI BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI MspA1I NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI