Restriction map for
pF1KSDA0814
(Linear) (Six-base) MAPSORT of: KIAA0814.seq Check: 2543 from: 1 to: 4569
KIAA0814.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2881 2914 3037 3304 3433 3543 4569
Size: 2881 33 123 267 129 110 1026
Fragments arranged by size:
2881 1026 267 129 123 110 33
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1018 2367 2537 3294 4569
Size: 343 675 1349 170 757 1275
Fragments arranged by size:
1349 1275 757 675 343 170
AfeI AGC'GCT
Cuts at: 0 536 4569
Size: 536 4033
AflIII A'CryG_T
Cuts at: 0 782 1190 2390 3053 4166 4569
Size: 782 408 1200 663 1113 403
Fragments arranged by size:
1200 1113 782 663 408 403
AhdI GACnn_n'nnGTC
Cuts at: 0 4357 4569
Size: 4357 212
AleI CACnn'nnGTG
Cuts at: 0 472 1543 2915 3031 4569
Size: 472 1071 1372 116 1538
Fragments arranged by size:
1538 1372 1071 472 116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2380 3424 4569
Size: 2380 1044 1145
Fragments arranged by size:
2380 1145 1044
AlwNI CAG_nnn'CTG
Cuts at: 0 321 1059 1203 1566 1755 3747 3975 4569
Size: 321 738 144 363 189 1992 228 594
Fragments arranged by size:
1992 738 594 363 321 228 189 144
ApaI G_GGCC'C
Cuts at: 0 1386 3261 3396 4451 4569
Size: 1386 1875 135 1055 118
Fragments arranged by size:
1875 1386 1055 135 118
ApaLI G'TGCA_C
Cuts at: 0 1786 2184 4500 4569
Size: 1786 398 2316 69
Fragments arranged by size:
2316 1786 398 69
AvaI C'yCGr_G
Cuts at: 0 7 167 444 1516 4103 4569
Size: 7 160 277 1072 2587 466
Fragments arranged by size:
2587 1072 466 277 160 7
AvrII C'CTAG_G
Cuts at: 0 2062 4569
Size: 2062 2507
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3042 3075 4569
Size: 3042 33 1494
Fragments arranged by size:
3042 1494 33
BamHI G'GATC_C
Cuts at: 0 440 4569
Size: 440 4129
BanI G'GyrC_C
Cuts at: 0 25 307 2066 2902 4416 4569
Size: 25 282 1759 836 1514 153
Fragments arranged by size:
1759 1514 836 282 153 25
BanII G_rGCy'C
Cuts at: 0 251 809 1265 1386 2167 2228 2666 3261
Size: 251 558 456 121 781 61 438 595
Cuts at: 3261 3396 3422 4289 4451 4569
Size: 135 26 867 162 118
Fragments arranged by size:
867 781 595 558 456 438 251 162
135 121 118 61 26
BbeI G_GCGC'C
Cuts at: 0 29 311 4569
Size: 29 282 4258
Fragments arranged by size:
4258 282 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 284 2584 4485 4569
Size: 284 2300 1901 84
Fragments arranged by size:
2300 1901 284 84
BbvCI CC'TCA_GC
Cuts at: 0 1834 2431 4569
Size: 1834 597 2138
Fragments arranged by size:
2138 1834 597
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 849 883 3004 3038 4569
Size: 849 34 2121 34 1531
Fragments arranged by size:
2121 1531 849 34 34
BfrBI ATG'CAT
Cuts at: 0 3914 4393 4569
Size: 3914 479 176
Fragments arranged by size:
3914 479 176
BglI GCCn_nnn'nGGC
Cuts at: 0 46 1814 2957 3305 3440 4070 4307 4569
Size: 46 1768 1143 348 135 630 237 262
Fragments arranged by size:
1768 1143 630 348 262 237 135 46
BlpI GC'TnA_GC
Cuts at: 0 188 2161 4236 4569
Size: 188 1973 2075 333
Fragments arranged by size:
2075 1973 333 188
Bme1580I G_kGCm'C
Cuts at: 0 790 1386 1516 1540 1790 2188 2303 2864
Size: 790 596 130 24 250 398 115 561
Cuts at: 2864 3261 3396 3883 4421 4451 4504 4569
Size: 397 135 487 538 30 53 65
Fragments arranged by size:
790 596 561 538 487 398 397 250
135 130 115 65 53 30 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 978 3255 3390 4163 4446 4569
Size: 978 2277 135 773 283 123
Fragments arranged by size:
2277 978 773 283 135 123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 101 3683 4569
Size: 101 3582 886
Fragments arranged by size:
3582 886 101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1025 1339 1660 1798 2748 3637 3778
Size: 71 954 314 321 138 950 889 141
Cuts at: 3778 4569
Size: 791
Fragments arranged by size:
954 950 889 791 321 314 141 138
71
Bpu10I CC'TnA_GC
Cuts at: 0 1455 1834 2344 2416 2431 2660 3119 4569
Size: 1455 379 510 72 15 229 459 1450
Fragments arranged by size:
1455 1450 510 459 379 229 72 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 2071 4569
Size: 235 1836 2498
Fragments arranged by size:
2498 1836 235
BsaI GGTCTCn'nnnn_
Cuts at: 0 1774 4569
Size: 1774 2795
BsaAI yAC'GTr
Cuts at: 0 783 854 3054 3504 3636 4569
Size: 783 71 2200 450 132 933
Fragments arranged by size:
2200 933 783 450 132 71
BsaBI GATnn'nnATC
Cuts at: 0 2098 4569
Size: 2098 2471
BsaHI Gr'CG_yC
Cuts at: 0 26 308 4569
Size: 26 282 4261
Fragments arranged by size:
4261 282 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 769 799 817 847 1133 1163 1207 1237
Size: 769 30 18 30 286 30 44 30
Cuts at: 1237 4569
Size: 3332
Fragments arranged by size:
3332 769 286 44 30 30 30 30
18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1141 1144 1851 2158 4503 4569
Size: 1141 3 707 307 2345 66
Fragments arranged by size:
2345 1141 707 307 66 3
BseYI C'CCAG_C
Cuts at: 0 147 244 789 2559 4569
Size: 147 97 545 1770 2010
Fragments arranged by size:
2010 1770 545 147 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 834 876 1768 2838 3430 3987 4569
Size: 787 47 42 892 1070 592 557 582
Fragments arranged by size:
1070 892 787 592 582 557 47 42
BsiHKAI G_wGCw'C
Cuts at: 0 394 1790 2188 4325 4504 4569
Size: 394 1396 398 2137 179 65
Fragments arranged by size:
2137 1396 398 394 179 65
Bsp1286I G_dGCh'C
Cuts at: 0 251 394 790 809 1265 1386 1516 1540
Size: 251 143 396 19 456 121 130 24
Cuts at: 1540 1790 2167 2188 2228 2303 2666 2864 3261
Size: 250 377 21 40 75 363 198 397
Cuts at: 3261 3396 3422 3883 4289 4325 4421 4451 4504
Size: 135 26 461 406 36 96 30 53
Cuts at: 4504 4569
Size: 65
Fragments arranged by size:
461 456 406 397 396 377 363 251
250 198 143 135 130 121 96 75
65 53 40 36 30 26 24 21
19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 124 491 1211 1469 1616 1682 2881 2914
Size: 124 367 720 258 147 66 1199 33
Cuts at: 2914 3037 3304 3433 3543 4569
Size: 123 267 129 110 1026
Fragments arranged by size:
1199 1026 720 367 267 258 147 129
124 123 110 66 33
BsrBI CCG'CTC
Cuts at: 0 4031 4569
Size: 4031 538
BsrDI GCAATG_nn'
Cuts at: 0 825 2502 2757 4569
Size: 825 1677 255 1812
Fragments arranged by size:
1812 1677 825 255
BsrFI r'CCGG_y
Cuts at: 0 1876 2426 3448 3856 3887 4078 4569
Size: 1876 550 1022 408 31 191 491
Fragments arranged by size:
1876 1022 550 491 408 191 31
BsrGI T'GTAC_A
Cuts at: 0 3680 4569
Size: 3680 889
BssHII G'CGCG_C
Cuts at: 0 36 4569
Size: 36 4533
BssSI C'ACGA_G
Cuts at: 0 1507 4569
Size: 1507 3062
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1493 2756 2852 4001 4010 4399 4569
Size: 1493 1263 96 1149 9 389 170
Fragments arranged by size:
1493 1263 1149 389 170 96 9
BstEII G'GTnAC_C
Cuts at: 0 591 4569
Size: 591 3978
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2932 3033 4569
Size: 2932 101 1536
Fragments arranged by size:
2932 1536 101
BstYI r'GATC_y
Cuts at: 0 431 440 551 617 4569
Size: 431 9 111 66 3952
Fragments arranged by size:
3952 431 111 66 9
BtgI C'CryG_G
Cuts at: 0 141 459 1803 2490 2666 3252 3341 3906
Size: 141 318 1344 687 176 586 89 565
Cuts at: 3906 4034 4134 4254 4569
Size: 128 100 120 315
Fragments arranged by size:
1344 687 586 565 318 315 176 141
128 120 100 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1058 2223 2625 4569
Size: 1058 1165 402 1944
Fragments arranged by size:
1944 1165 1058 402
BtsI GCAGTG_nn'
Cuts at: 0 2188 3815 4267 4323 4569
Size: 2188 1627 452 56 246
Fragments arranged by size:
2188 1627 452 246 56
DraIII CAC_nnn'GTG
Cuts at: 0 110 3114 3720 4010 4569
Size: 110 3004 606 290 559
Fragments arranged by size:
3004 606 559 290 110
DrdI GACnn_nn'nnGTC
Cuts at: 0 286 4569
Size: 286 4283
EaeI y'GGCC_r
Cuts at: 0 708 1343 2111 2741 3557 4119 4569
Size: 708 635 768 630 816 562 450
Fragments arranged by size:
816 768 708 635 630 562 450
EarI CTCTTCn'nnn_
Cuts at: 0 1270 2032 4520 4569
Size: 1270 762 2488 49
Fragments arranged by size:
2488 1270 762 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 775 2058 2304 3056 3507 4363 4497 4569
Size: 775 1283 246 752 451 856 134 72
Fragments arranged by size:
1283 856 775 752 451 246 134 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 343 1018 1025 1339 1660 1798 2367
Size: 71 272 675 7 314 321 138 569
Cuts at: 2367 2537 2748 3294 3637 3778 4569
Size: 170 211 546 343 141 791
Fragments arranged by size:
791 675 569 546 343 321 314 272
211 170 141 138 71 7
EcoO109I rG'GnC_Cy
Cuts at: 0 318 1056 1200 1382 1940 2799 3257 3392
Size: 318 738 144 182 558 859 458 135
Cuts at: 3392 3957 4107 4157 4448 4569
Size: 565 150 50 291 121
Fragments arranged by size:
859 738 565 558 458 318 291 182
150 144 135 121 50
EcoRV GAT'ATC
Cuts at: 0 1028 4569
Size: 1028 3541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 255 1281 4569
Size: 255 1026 3288
Fragments arranged by size:
3288 1026 255
FspI TGC'GCA
Cuts at: 0 3928 4569
Size: 3928 641
FspAI rTGC'GCAy
Cuts at: 0 3928 4569
Size: 3928 641
HaeII r_GCGC'y
Cuts at: 0 29 52 64 195 311 538 2560 4569
Size: 29 23 12 131 116 227 2022 2009
Fragments arranged by size:
2022 2009 227 131 116 29 23 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 609 641 796 828 2524 2556 4343 4355
Size: 609 32 155 32 1696 32 1787 12
Cuts at: 4355 4375 4387 4498 4530 4569
Size: 20 12 111 32 39
Fragments arranged by size:
1787 1696 609 155 111 39 32 32
32 32 20 12 12
HincII GTy'rAC
Cuts at: 0 3480 4569
Size: 3480 1089
KasI G'GCGC_C
Cuts at: 0 25 307 4569
Size: 25 282 4262
Fragments arranged by size:
4262 282 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1580 2009 2346 2585 3552 3694 4569
Size: 665 915 429 337 239 967 142 875
Fragments arranged by size:
967 915 875 665 429 337 239 142
MscI TGG'CCA
Cuts at: 0 710 2113 2743 3559 4569
Size: 710 1403 630 816 1010
Fragments arranged by size:
1403 1010 816 710 630
MslI CAynn'nnrTG
Cuts at: 0 232 472 1317 1543 2191 2362 2495 2915
Size: 232 240 845 226 648 171 133 420
Cuts at: 2915 2930 3031 3406 4167 4569
Size: 15 101 375 761 402
Fragments arranged by size:
845 761 648 420 402 375 240 232
226 171 133 101 15
MspA1I CmG'CkG
Cuts at: 0 124 461 509 1146 1388 1451 1776 2160
Size: 124 337 48 637 242 63 325 384
Cuts at: 2160 2648 2819 2942 2973 3176 3200 3431 4569
Size: 488 171 123 31 203 24 231 1138
Fragments arranged by size:
1138 637 488 384 337 325 242 231
203 171 124 123 63 48 31 24
NaeI GCC'GGC
Cuts at: 0 1878 3450 4569
Size: 1878 1572 1119
Fragments arranged by size:
1878 1572 1119
NarI GG'CG_CC
Cuts at: 0 26 308 4569
Size: 26 282 4261
Fragments arranged by size:
4261 282 26
NcoI C'CATG_G
Cuts at: 0 2490 2666 3341 4134 4254 4569
Size: 2490 176 675 793 120 315
Fragments arranged by size:
2490 793 675 315 176 120
NgoMIV G'CCGG_C
Cuts at: 0 1876 3448 4569
Size: 1876 1572 1121
Fragments arranged by size:
1876 1572 1121
NsiI A_TGCA'T
Cuts at: 0 3916 4395 4569
Size: 3916 479 174
Fragments arranged by size:
3916 479 174
NspI r_CATG'y
Cuts at: 0 612 2250 2367 2394 2792 3293 4170 4569
Size: 612 1638 117 27 398 501 877 399
Fragments arranged by size:
1638 877 612 501 399 398 117 27
PasI CC'CwG_GG
Cuts at: 0 1936 3303 3975 4569
Size: 1936 1367 672 594
Fragments arranged by size:
1936 1367 672 594
PciI A'CATG_T
Cuts at: 0 2390 4166 4569
Size: 2390 1776 403
Fragments arranged by size:
2390 1776 403
PflMI CCAn_nnn'nTGG
Cuts at: 0 147 1566 2109 3267 4569
Size: 147 1419 543 1158 1302
Fragments arranged by size:
1419 1302 1158 543 147
PfoI T'CCnGG_A
Cuts at: 0 314 1355 2102 2117 4569
Size: 314 1041 747 15 2452
Fragments arranged by size:
2452 1041 747 314 15
PmlI CAC'GTG
Cuts at: 0 854 3636 4569
Size: 854 2782 933
Fragments arranged by size:
2782 933 854
PpuMI rG'GwC_Cy
Cuts at: 0 318 1200 2799 4107 4157 4569
Size: 318 882 1599 1308 50 412
Fragments arranged by size:
1599 1308 882 412 318 50
PshAI GACnn'nnGTC
Cuts at: 0 1899 2588 4569
Size: 1899 689 1981
Fragments arranged by size:
1981 1899 689
PspOMI G'GGCC_C
Cuts at: 0 1382 3257 3392 4447 4569
Size: 1382 1875 135 1055 122
Fragments arranged by size:
1875 1382 1055 135 122
PstI C_TGCA'G
Cuts at: 0 972 1208 1247 1280 1393 2590 2653 3356
Size: 972 236 39 33 113 1197 63 703
Cuts at: 3356 3554 3947 4569
Size: 198 393 622
Fragments arranged by size:
1197 972 703 622 393 236 198 113
63 39 33
PvuII CAG'CTG
Cuts at: 0 509 1146 1776 2160 2942 3176 4569
Size: 509 637 630 384 782 234 1393
Fragments arranged by size:
1393 782 637 630 509 384 234
RsrII CG'GwC_CG
Cuts at: 0 3885 4569
Size: 3885 684
SacII CC_GC'GG
Cuts at: 0 462 4569
Size: 462 4107
SanDI GG'GwC_CC
Cuts at: 0 4107 4569
Size: 4107 462
SapI GCTCTTCn'nnn_
Cuts at: 0 1270 4569
Size: 1270 3299
SbfI CC_TGCA'GG
Cuts at: 0 972 3554 4569
Size: 972 2582 1015
Fragments arranged by size:
2582 1015 972
SexAI A'CCwGG_T
Cuts at: 0 284 4569
Size: 284 4285
SfcI C'TryA_G
Cuts at: 0 968 1141 1204 1243 1276 1389 2586 2649
Size: 968 173 63 39 33 113 1197 63
Cuts at: 2649 2832 3195 3352 3550 3943 4569
Size: 183 363 157 198 393 626
Fragments arranged by size:
1197 968 626 393 363 198 183 173
157 113 63 63 39 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1814 4569
Size: 1814 2755
SfoI GGC'GCC
Cuts at: 0 27 309 4569
Size: 27 282 4260
Fragments arranged by size:
4260 282 27
SmaI CCC'GGG
Cuts at: 0 9 4105 4569
Size: 9 4096 464
Fragments arranged by size:
4096 464 9
SmlI C'TyrA_G
Cuts at: 0 250 2086 4569
Size: 250 1836 2483
Fragments arranged by size:
2483 1836 250
SphI G_CATG'C
Cuts at: 0 2250 2367 4569
Size: 2250 117 2202
Fragments arranged by size:
2250 2202 117
StyI C'CwwG_G
Cuts at: 0 56 1112 2062 2252 2490 2666 3341 3521
Size: 56 1056 950 190 238 176 675 180
Cuts at: 3521 3834 4134 4254 4278 4412 4569
Size: 313 300 120 24 134 157
Fragments arranged by size:
1056 950 675 313 300 238 190 180
176 157 134 120 56 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 997 2278 2601 2809 3354 4569
Size: 997 1281 323 208 545 1215
Fragments arranged by size:
1281 1215 997 545 323 208
TatI w'GTAC_w
Cuts at: 0 650 986 3680 4569
Size: 650 336 2694 889
Fragments arranged by size:
2694 889 650 336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 769 801 1495 1527 2037 2069 3195 3227
Size: 769 32 694 32 510 32 1126 32
Cuts at: 3227 3329 3361 4130 4162 4569
Size: 102 32 769 32 407
Fragments arranged by size:
1126 769 769 694 510 407 102 32
32 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1563 2106 3310 4569
Size: 1563 543 1204 1259
Fragments arranged by size:
1563 1259 1204 543
XmaI C'CCGG_G
Cuts at: 0 7 4103 4569
Size: 7 4096 466
Fragments arranged by size:
4096 466 7
XmnI GAAnn'nnTTC
Cuts at: 0 454 4569
Size: 454 4115
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BfrBI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI
EarI EciI Eco57MI EcoO109I EcoRV FalI FspI FspAI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII
RsrII SacII SanDI SapI SbfI SexAI SfcI SfiI
SfoI SmaI SmlI SphI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AloI ApoI
AscI AseI AsiSI BciVI BclI BglII BmgBI BmtI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BstBI
BstZ17I Bsu36I ClaI CspCI DraI EagI EcoICRI EcoNI
EcoRI FseI HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI PacI PmeI PpiI PsiI PspXI
PsrI PvuI SacI SalI ScaI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI
ZraI