Restriction map for
pF1KA0815
(Linear) (Six-base) MAPSORT of: KIAA0815.seq Check: 1598 from: 1 to: 3687
KIAA0815.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 825 1612 1792 2491 3130 3214 3388 3687
Size: 825 787 180 699 639 84 174 299
Fragments arranged by size:
825 787 699 639 299 180 174 84
AatII G_ACGT'C
Cuts at: 0 1154 2669 3687
Size: 1154 1515 1018
Fragments arranged by size:
1515 1154 1018
AccI GT'mk_AC
Cuts at: 0 803 3687
Size: 803 2884
AfeI AGC'GCT
Cuts at: 0 1726 2313 2818 3687
Size: 1726 587 505 869
Fragments arranged by size:
1726 869 587 505
AflIII A'CryG_T
Cuts at: 0 751 2129 3074 3358 3687
Size: 751 1378 945 284 329
Fragments arranged by size:
1378 945 751 329 284
AgeI A'CCGG_T
Cuts at: 0 206 3179 3687
Size: 206 2973 508
Fragments arranged by size:
2973 508 206
AleI CACnn'nnGTG
Cuts at: 0 191 332 731 3073 3077 3687
Size: 191 141 399 2342 4 610
Fragments arranged by size:
2342 610 399 191 141 4
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 466 1800 3687
Size: 466 1334 1887
Fragments arranged by size:
1887 1334 466
AlwNI CAG_nnn'CTG
Cuts at: 0 281 2120 2561 3687
Size: 281 1839 441 1126
Fragments arranged by size:
1839 1126 441 281
ApaI G_GGCC'C
Cuts at: 0 743 1932 2377 2417 2635 3476 3566 3587
Size: 743 1189 445 40 218 841 90 21
Cuts at: 3587 3687
Size: 100
Fragments arranged by size:
1189 841 743 445 218 100 90 40
21
ApaLI G'TGCA_C
Cuts at: 0 747 840 3687
Size: 747 93 2847
Fragments arranged by size:
2847 747 93
AvaI C'yCGr_G
Cuts at: 0 11 376 1312 1443 1905 2005 2230 2660
Size: 11 365 936 131 462 100 225 430
Cuts at: 2660 2917 3016 3135 3598 3687
Size: 257 99 119 463 89
Fragments arranged by size:
936 463 462 430 365 257 225 131
119 100 99 89 11
BanI G'GyrC_C
Cuts at: 0 38 458 637 1538 2503 3118 3376 3687
Size: 38 420 179 901 965 615 258 311
Fragments arranged by size:
965 901 615 420 311 258 179 38
BanII G_rGCy'C
Cuts at: 0 50 743 1331 1580 1912 1932 2377 2417
Size: 50 693 588 249 332 20 445 40
Cuts at: 2417 2635 2779 2971 3476 3566 3587 3625 3687
Size: 218 144 192 505 90 21 38 62
Fragments arranged by size:
693 588 505 445 332 249 218 192
144 90 62 50 40 38 21 20
BbeI G_GCGC'C
Cuts at: 0 641 3687
Size: 641 3046
BbsI GAAGACnn'nnnn_
Cuts at: 0 1991 3687
Size: 1991 1696
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 783 793 817 827 3687
Size: 783 10 24 10 2860
Fragments arranged by size:
2860 783 24 10 10
BglI GCCn_nnn'nGGC
Cuts at: 0 131 500 947 1289 1298 1316 1484 1793
Size: 131 369 447 342 9 18 168 309
Cuts at: 1793 2102 2141 2492 2527 2531 2621 2630 2750
Size: 309 39 351 35 4 90 9 120
Cuts at: 2750 2789 2989 3056 3389 3591 3687
Size: 39 200 67 333 202 96
Fragments arranged by size:
447 369 351 342 333 309 309 202
200 168 131 120 96 90 67 39
39 35 18 9 9 4
BglII A'GATC_T
Cuts at: 0 2036 3687
Size: 2036 1651
BlpI GC'TnA_GC
Cuts at: 0 3624 3687
Size: 3624 63
Bme1580I G_kGCm'C
Cuts at: 0 79 576 743 751 844 1879 1932 2137
Size: 79 497 167 8 93 1035 53 205
Cuts at: 2137 2195 2229 2276 2377 2417 2506 2635 2814
Size: 58 34 47 101 40 89 129 179
Cuts at: 2814 3379 3476 3566 3587 3634 3687
Size: 565 97 90 21 47 53
Fragments arranged by size:
1035 565 497 205 179 167 129 101
97 93 90 89 79 58 53 53
47 47 40 34 21 8
BmgBI CAC'GTC
Cuts at: 0 2130 2466 2853 3687
Size: 2130 336 387 834
Fragments arranged by size:
2130 834 387 336
BmrI ACTGGGnnnn_n'
Cuts at: 0 561 1334 1783 1988 2279 2286 2768 2867
Size: 561 773 449 205 291 7 482 99
Cuts at: 2867 3017 3687
Size: 150 670
Fragments arranged by size:
773 670 561 482 449 291 205 150
99 7
BmtI G_CTAG'C
Cuts at: 0 514 3687
Size: 514 3173
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 951 3687
Size: 951 2736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 373 921 1066 1099 2517 3352 3630
Size: 117 256 548 145 33 1418 835 278
Cuts at: 3630 3687
Size: 57
Fragments arranged by size:
1418 835 548 278 256 145 117 57
33
Bpu10I CC'TnA_GC
Cuts at: 0 3429 3612 3687
Size: 3429 183 75
Fragments arranged by size:
3429 183 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 2071 3687
Size: 2071 1616
BsaAI yAC'GTr
Cuts at: 0 498 2370 3075 3359 3569 3687
Size: 498 1872 705 284 210 118
Fragments arranged by size:
1872 705 498 284 210 118
BsaBI GATnn'nnATC
Cuts at: 0 673 3687
Size: 673 3014
BsaHI Gr'CG_yC
Cuts at: 0 7 638 1151 2666 3149 3687
Size: 7 631 513 1515 483 538
Fragments arranged by size:
1515 631 538 513 483 7
BsaWI w'CCGG_w
Cuts at: 0 206 659 1353 2080 2209 3179 3687
Size: 206 453 694 727 129 970 508
Fragments arranged by size:
970 727 694 508 453 206 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1335 1365 1633 1663 2953 2983 3193 3223
Size: 1335 30 268 30 1290 30 210 30
Cuts at: 3223 3687
Size: 464
Fragments arranged by size:
1335 1290 464 268 210 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1063 1654 3687
Size: 1063 591 2033
Fragments arranged by size:
2033 1063 591
BseYI C'CCAG_C
Cuts at: 0 633 736 1746 2015 2022 2043 2136 2155
Size: 633 103 1010 269 7 21 93 19
Cuts at: 2155 2202 2745 2813 3475 3687
Size: 47 543 68 662 212
Fragments arranged by size:
1010 662 633 543 269 212 103 93
68 47 21 19 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 948 1000 1675 2463 2553 3051 3465
Size: 833 115 52 675 788 90 498 414
Cuts at: 3465 3472 3687
Size: 7 215
Fragments arranged by size:
833 788 675 498 414 215 115 90
52 7
BsiEI CG_ry'CG
Cuts at: 0 1587 2492 2531 3687
Size: 1587 905 39 1156
Fragments arranged by size:
1587 1156 905 39
BsiHKAI G_wGCw'C
Cuts at: 0 751 844 959 994 1115 1912 2176 3203
Size: 751 93 115 35 121 797 264 1027
Cuts at: 3203 3340 3422 3687
Size: 137 82 265
Fragments arranged by size:
1027 797 751 265 264 137 121 115
93 82 35
BsmI GAATG_Cn'
Cuts at: 0 181 3687
Size: 181 3506
Bsp1286I G_dGCh'C
Cuts at: 0 50 79 576 743 751 844 959 994
Size: 50 29 497 167 8 93 115 35
Cuts at: 994 1115 1331 1580 1879 1912 1932 2137 2176
Size: 121 216 249 299 33 20 205 39
Cuts at: 2176 2195 2229 2276 2377 2417 2506 2635 2779
Size: 19 34 47 101 40 89 129 144
Cuts at: 2779 2814 2971 3203 3340 3379 3422 3476 3566
Size: 35 157 232 137 39 43 54 90
Cuts at: 3566 3587 3625 3634 3687
Size: 21 38 9 53
Fragments arranged by size:
497 299 249 232 216 205 167 157
144 137 129 121 115 101 93 90
89 54 53 50 47 43 40 39
39 38 35 35 34 33 29 21
20 19 9 8
BspEI T'CCGG_A
Cuts at: 0 1353 3687
Size: 1353 2334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 289 799 825 1288 1417 1612 1792 2491
Size: 289 510 26 463 129 195 180 699
Cuts at: 2491 3130 3214 3388 3687
Size: 639 84 174 299
Fragments arranged by size:
699 639 510 463 299 289 195 180
174 129 84 26
BsrBI CCG'CTC
Cuts at: 0 1817 3668 3687
Size: 1817 1851 19
Fragments arranged by size:
1851 1817 19
BsrDI GCAATG_nn'
Cuts at: 0 318 1103 1344 3687
Size: 318 785 241 2343
Fragments arranged by size:
2343 785 318 241
BsrFI r'CCGG_y
Cuts at: 0 130 148 206 371 647 880 887 976
Size: 130 18 58 165 276 233 7 89
Cuts at: 976 1003 1010 1535 1958 2239 2491 2530 2681
Size: 27 7 525 423 281 252 39 151
Cuts at: 2681 2749 3115 3145 3179 3422 3687
Size: 68 366 30 34 243 265
Fragments arranged by size:
525 423 366 281 276 265 252 243
233 165 151 130 89 68 58 39
34 30 27 18 7 7
BssHII G'CGCG_C
Cuts at: 0 64 3687
Size: 64 3623
BssSI C'ACGA_G
Cuts at: 0 1018 3687
Size: 1018 2669
BstAPI GCAn_nnn'nTGC
Cuts at: 0 281 1514 2120 2510 2561 3687
Size: 281 1233 606 390 51 1126
Fragments arranged by size:
1233 1126 606 390 281 51
BstEII G'GTnAC_C
Cuts at: 0 151 3687
Size: 151 3536
BstXI CCAn_nnnn'nTGG
Cuts at: 0 193 2144 2753 2857 3079 3687
Size: 193 1951 609 104 222 608
Fragments arranged by size:
1951 609 608 222 193 104
BstYI r'GATC_y
Cuts at: 0 2036 3687
Size: 2036 1651
BtgI C'CryG_G
Cuts at: 0 18 576 714 759 942 1612 1938 2319
Size: 18 558 138 45 183 670 326 381
Cuts at: 2319 2377 2984 3371 3687
Size: 58 607 387 316
Fragments arranged by size:
670 607 558 387 381 326 316 183
138 58 45 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 343 3687
Size: 343 3344
BtsI GCAGTG_nn'
Cuts at: 0 658 960 1960 2193 2214 2398 2562 3282
Size: 658 302 1000 233 21 184 164 720
Cuts at: 3282 3541 3634 3687
Size: 259 93 53
Fragments arranged by size:
1000 720 658 302 259 233 184 164
93 53 21
ClaI AT'CG_AT
Cuts at: 0 797 3687
Size: 797 2890
DraIII CAC_nnn'GTG
Cuts at: 0 3546 3687
Size: 3546 141
EaeI y'GGCC_r
Cuts at: 0 26 287 645 974 1233 1584 1866 2316
Size: 26 261 358 329 259 351 282 450
Cuts at: 2316 2489 2528 2622 2981 3405 3687
Size: 173 39 94 359 424 282
Fragments arranged by size:
450 424 359 358 351 329 282 282
261 259 173 94 39 26
EagI C'GGCC_G
Cuts at: 0 1584 2489 2528 3687
Size: 1584 905 39 1159
Fragments arranged by size:
1584 1159 905 39
EarI CTCTTCn'nnn_
Cuts at: 0 1141 1163 3687
Size: 1141 22 2524
Fragments arranged by size:
2524 1141 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2903 3687
Size: 2903 784
EcoICRI GAG'CTC
Cuts at: 0 1910 3687
Size: 1910 1777
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 373 921 1066 1099 2517 3352 3630
Size: 117 256 548 145 33 1418 835 278
Cuts at: 3630 3687
Size: 57
Fragments arranged by size:
1418 835 548 278 256 145 117 57
33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 124 3687
Size: 124 3563
EcoO109I rG'GnC_Cy
Cuts at: 0 57 278 474 599 740 788 1277 1406
Size: 57 221 196 125 141 48 489 129
Cuts at: 1406 1427 1556 1928 1929 2281 2474 2631 2632
Size: 21 129 372 1 352 193 157 1
Cuts at: 2632 2709 2861 2990 3225 3562 3584 3687
Size: 77 152 129 235 337 22 103
Fragments arranged by size:
489 372 352 337 235 221 196 193
157 152 141 129 129 129 125 103
77 57 48 22 21 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 270 1728 2192 3687
Size: 270 1458 464 1495
Fragments arranged by size:
1495 1458 464 270
HaeII r_GCGC'y
Cuts at: 0 149 641 1728 2315 2820 3312 3429 3687
Size: 149 492 1087 587 505 492 117 258
Fragments arranged by size:
1087 587 505 492 492 258 149 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1335 1367 3134 3166 3592 3624 3687
Size: 1335 32 1767 32 426 32 63
Fragments arranged by size:
1767 1335 426 63 32 32 32
HincII GTy'rAC
Cuts at: 0 804 3687
Size: 804 2883
KasI G'GCGC_C
Cuts at: 0 637 3687
Size: 637 3050
MscI TGG'CCA
Cuts at: 0 289 1235 1868 2318 3407 3687
Size: 289 946 633 450 1089 280
Fragments arranged by size:
1089 946 633 450 289 280
MslI CAynn'nnrTG
Cuts at: 0 191 332 731 1108 3073 3077 3687
Size: 191 141 399 377 1965 4 610
Fragments arranged by size:
1965 610 399 377 191 141 4
MspA1I CmG'CkG
Cuts at: 0 20 446 485 517 686 761 1391 1436
Size: 20 426 39 32 169 75 630 45
Cuts at: 1436 1694 2015 2066 2120 2159 2222 2342 2379
Size: 258 321 51 54 39 63 120 37
Cuts at: 2379 2430 2471 2620 2639 2690 2735 2825 2839
Size: 51 41 149 19 51 45 90 14
Cuts at: 2839 2870 2946 2999 3155 3251 3386 3479 3628
Size: 31 76 53 156 96 135 93 149
Cuts at: 3628 3687
Size: 59
Fragments arranged by size:
630 426 321 258 169 156 149 149
135 120 96 93 90 76 75 63
59 54 53 51 51 51 45 45
41 39 39 37 32 31 20 19
14
NaeI GCC'GGC
Cuts at: 0 132 373 649 882 978 1005 2493 2532
Size: 132 241 276 233 96 27 1488 39
Cuts at: 2532 2683 2751 3147 3687
Size: 151 68 396 540
Fragments arranged by size:
1488 540 396 276 241 233 151 132
96 68 39 27
NarI GG'CG_CC
Cuts at: 0 638 3687
Size: 638 3049
NcoI C'CATG_G
Cuts at: 0 576 714 3687
Size: 576 138 2973
Fragments arranged by size:
2973 576 138
NgoMIV G'CCGG_C
Cuts at: 0 130 371 647 880 976 1003 2491 2530
Size: 130 241 276 233 96 27 1488 39
Cuts at: 2530 2681 2749 3145 3687
Size: 151 68 396 542
Fragments arranged by size:
1488 542 396 276 241 233 151 130
96 68 39 27
NheI G'CTAG_C
Cuts at: 0 510 3687
Size: 510 3177
NspI r_CATG'y
Cuts at: 0 453 755 1781 2051 2521 3687
Size: 453 302 1026 270 470 1166
Fragments arranged by size:
1166 1026 470 453 302 270
PasI CC'CwG_GG
Cuts at: 0 306 594 1573 2400 3304 3618 3687
Size: 306 288 979 827 904 314 69
Fragments arranged by size:
979 904 827 314 306 288 69
PciI A'CATG_T
Cuts at: 0 751 3687
Size: 751 2936
PfoI T'CCnGG_A
Cuts at: 0 389 1115 1139 3661 3687
Size: 389 726 24 2522 26
Fragments arranged by size:
2522 726 389 26 24
PmlI CAC'GTG
Cuts at: 0 498 3075 3359 3569 3687
Size: 498 2577 284 210 118
Fragments arranged by size:
2577 498 284 210 118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1181 1213 3687
Size: 1181 32 2474
Fragments arranged by size:
2474 1181 32
PpuMI rG'GwC_Cy
Cuts at: 0 278 599 788 1277 1406 2281 3687
Size: 278 321 189 489 129 875 1406
Fragments arranged by size:
1406 875 489 321 278 189 129
PshAI GACnn'nnGTC
Cuts at: 0 2768 3687
Size: 2768 919
PspOMI G'GGCC_C
Cuts at: 0 739 1928 2373 2413 2631 3472 3562 3583
Size: 739 1189 445 40 218 841 90 21
Cuts at: 3583 3687
Size: 104
Fragments arranged by size:
1189 841 739 445 218 104 90 40
21
PspXI vC'TCGA_Gb
Cuts at: 0 1905 3687
Size: 1905 1782
PstI C_TGCA'G
Cuts at: 0 724 1039 1246 1267 1378 1390 2221 2293
Size: 724 315 207 21 111 12 831 72
Cuts at: 2293 2311 2824 3217 3687
Size: 18 513 393 470
Fragments arranged by size:
831 724 513 470 393 315 207 111
72 21 18 12
PvuII CAG'CTG
Cuts at: 0 446 686 1391 1694 2159 2222 2430 2825
Size: 446 240 705 303 465 63 208 395
Cuts at: 2825 2946 3251 3479 3687
Size: 121 305 228 208
Fragments arranged by size:
705 465 446 395 305 303 240 228
208 208 121 63
SacI G_AGCT'C
Cuts at: 0 1912 3687
Size: 1912 1775
SacII CC_GC'GG
Cuts at: 0 21 762 2380 3687
Size: 21 741 1618 1307
Fragments arranged by size:
1618 1307 741 21
SalI G'TCGA_C
Cuts at: 0 802 3687
Size: 802 2885
SfcI C'TryA_G
Cuts at: 0 720 1035 1242 1263 1374 1386 2217 2289
Size: 720 315 207 21 111 12 831 72
Cuts at: 2289 2307 2820 3213 3687
Size: 18 513 393 474
Fragments arranged by size:
831 720 513 474 393 315 207 111
72 21 18 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2630 2989 3056 3687
Size: 2630 359 67 631
Fragments arranged by size:
2630 631 359 67
SfoI GGC'GCC
Cuts at: 0 639 3687
Size: 639 3048
SgrAI Cr'CCGG_yG
Cuts at: 0 148 206 3422 3687
Size: 148 58 3216 265
Fragments arranged by size:
3216 265 148 58
SmaI CCC'GGG
Cuts at: 0 13 378 1445 2232 2662 2919 3137 3687
Size: 13 365 1067 787 430 257 218 550
Fragments arranged by size:
1067 787 550 430 365 257 218 13
SmlI C'TyrA_G
Cuts at: 0 1905 3687
Size: 1905 1782
SphI G_CATG'C
Cuts at: 0 453 1781 2051 2521 3687
Size: 453 1328 270 470 1166
Fragments arranged by size:
1328 1166 470 453 270
SrfI GCCC'GGGC
Cuts at: 0 2919 3687
Size: 2919 768
StuI AGG'CCT
Cuts at: 0 529 2806 3412 3687
Size: 529 2277 606 275
Fragments arranged by size:
2277 606 529 275
StyI C'CwwG_G
Cuts at: 0 42 576 714 1481 3687
Size: 42 534 138 767 2206
Fragments arranged by size:
2206 767 534 138 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 607 2216 2942 3071 3085 3687
Size: 607 1609 726 129 14 602
Fragments arranged by size:
1609 726 607 602 129 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1633 1654 1665 1686 3193 3225 3687
Size: 1633 21 11 21 1507 32 462
Fragments arranged by size:
1633 1507 462 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 0 1150 1417 2464 3523 3687
Size: 1150 267 1047 1059 164
Fragments arranged by size:
1150 1059 1047 267 164
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 574 733 2407 2443 2854 3181 3444 3687
Size: 574 159 1674 36 411 327 263 243
Fragments arranged by size:
1674 574 411 327 263 243 159 36
XhoI C'TCGA_G
Cuts at: 0 1905 3687
Size: 1905 1782
XmaI C'CCGG_G
Cuts at: 0 11 376 1443 2230 2660 2917 3135 3687
Size: 11 365 1067 787 430 257 218 552
Fragments arranged by size:
1067 787 552 430 365 257 218 11
ZraI GAC'GTC
Cuts at: 0 1152 2667 3687
Size: 1152 1515 1020
Fragments arranged by size:
1515 1152 1020
Enzymes that do cut:
AarI AatII AccI AfeI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SacII SalI SfcI SfiI SfoI SgrAI
SmaI SmlI SphI SrfI StuI StyI TaqII TstI
Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AflII AhdI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BpuEI BsiWI BsmBI BspHI BsrGI BstBI BstZ17I
Bsu36I CspCI DraI DrdI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MmeI NdeI
NotI NruI NsiI PacI PflMI PmeI PsiI PsrI
PvuI RsrII SanDI SapI SbfI ScaI SexAI SgfI
SnaBI SpeI SspI SwaI TatI XbaI XmnI