Restriction map for
pF1KA0819
(Linear) (Six-base) MAPSORT of: KIAA0819.seq Check: 7894 from: 1 to: 5754
KIAA0819.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 79 5316 5754
Size: 79 5237 438
Fragments arranged by size:
5237 438 79
AatII G_ACGT'C
Cuts at: 0 2792 5754
Size: 2792 2962
AccI GT'mk_AC
Cuts at: 0 2312 5532 5754
Size: 2312 3220 222
Fragments arranged by size:
3220 2312 222
Acc65I G'GTAC_C
Cuts at: 0 2724 5754
Size: 2724 3030
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1362 3804 4756 5721 5754
Size: 1362 2442 952 965 33
Fragments arranged by size:
2442 1362 965 952 33
AflII C'TTAA_G
Cuts at: 0 5281 5578 5754
Size: 5281 297 176
Fragments arranged by size:
5281 297 176
AgeI A'CCGG_T
Cuts at: 0 50 5754
Size: 50 5704
AhdI GACnn_n'nnGTC
Cuts at: 0 3075 5019 5754
Size: 3075 1944 735
Fragments arranged by size:
3075 1944 735
AleI CACnn'nnGTG
Cuts at: 0 3772 5754
Size: 3772 1982
AlwNI CAG_nnn'CTG
Cuts at: 0 831 1050 2187 2271 4572 5754
Size: 831 219 1137 84 2301 1182
Fragments arranged by size:
2301 1182 1137 831 219 84
ApaI G_GGCC'C
Cuts at: 0 3284 3794 4792 5754
Size: 3284 510 998 962
Fragments arranged by size:
3284 998 962 510
ApaLI G'TGCA_C
Cuts at: 0 249 610 3403 5754
Size: 249 361 2793 2351
Fragments arranged by size:
2793 2351 361 249
ApoI r'AATT_y
Cuts at: 0 544 643 706 741 817 1512 2194 2431
Size: 544 99 63 35 76 695 682 237
Cuts at: 2431 4372 5754
Size: 1941 1382
Fragments arranged by size:
1941 1382 695 682 544 237 99 76
63 35
AvaI C'yCGr_G
Cuts at: 0 977 1235 2078 3760 3793 4140 4241 4405
Size: 977 258 843 1682 33 347 101 164
Cuts at: 4405 5501 5754
Size: 1096 253
Fragments arranged by size:
1682 1096 977 843 347 258 253 164
101 33
AvrII C'CTAG_G
Cuts at: 0 516 5754
Size: 516 5238
BamHI G'GATC_C
Cuts at: 0 2903 5754
Size: 2903 2851
BanI G'GyrC_C
Cuts at: 0 421 1580 2542 2656 2724 2776 4630 5111
Size: 421 1159 962 114 68 52 1854 481
Cuts at: 5111 5754
Size: 643
Fragments arranged by size:
1854 1159 962 643 481 421 114 68
52
BanII G_rGCy'C
Cuts at: 0 104 968 1300 2245 2633 3284 3472 3767
Size: 104 864 332 945 388 651 188 295
Cuts at: 3767 3794 3800 4048 4154 4190 4720 4730 4792
Size: 27 6 248 106 36 530 10 62
Cuts at: 4792 4822 5301 5549 5754
Size: 30 479 248 205
Fragments arranged by size:
945 864 651 530 479 388 332 295
248 248 205 188 106 104 62 36
30 27 10 6
BbeI G_GCGC'C
Cuts at: 0 2546 2780 4634 5115 5754
Size: 2546 234 1854 481 639
Fragments arranged by size:
2546 1854 639 481 234
BbsI GAAGACnn'nnnn_
Cuts at: 0 4066 4968 5525 5754
Size: 4066 902 557 229
Fragments arranged by size:
4066 902 557 229
BbvCI CC'TCA_GC
Cuts at: 0 2708 3116 3139 4656 5754
Size: 2708 408 23 1517 1098
Fragments arranged by size:
2708 1517 1098 408 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2166 2200 4174 4208 5754
Size: 2166 34 1974 34 1546
Fragments arranged by size:
2166 1974 1546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 484 1421 5754
Size: 484 937 4333
Fragments arranged by size:
4333 937 484
BclI T'GATC_A
Cuts at: 0 653 5754
Size: 653 5101
BglI GCCn_nnn'nGGC
Cuts at: 0 196 736 1021 1212 4796 5754
Size: 196 540 285 191 3584 958
Fragments arranged by size:
3584 958 540 285 196 191
BglII A'GATC_T
Cuts at: 0 1623 5754
Size: 1623 4131
BlpI GC'TnA_GC
Cuts at: 0 1006 4253 4597 5119 5754
Size: 1006 3247 344 522 635
Fragments arranged by size:
3247 1006 635 522 344
Bme1580I G_kGCm'C
Cuts at: 0 253 606 614 2659 3112 3284 3407 3794
Size: 253 353 8 2045 453 172 123 387
Cuts at: 3794 4792 5754
Size: 998 962
Fragments arranged by size:
2045 998 962 453 387 353 253 172
123 8
BmgBI CAC'GTC
Cuts at: 0 534 5754
Size: 534 5220
BmrI ACTGGGnnnn_n'
Cuts at: 0 331 3570 3679 4075 5754
Size: 331 3239 109 396 1679
Fragments arranged by size:
3239 1679 396 331 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4641 4782 5187 5754
Size: 4641 141 405 567
Fragments arranged by size:
4641 567 405 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 2644 2698 3154 4101 4480 4806 5025
Size: 473 2171 54 456 947 379 326 219
Cuts at: 5025 5130 5371 5530 5650 5689 5731 5754
Size: 105 241 159 120 39 42 23
Fragments arranged by size:
2171 947 473 456 379 326 241 219
159 120 105 54 42 39 23
Bpu10I CC'TnA_GC
Cuts at: 0 1960 2708 2755 3116 3139 3344 4174 4656
Size: 1960 748 47 361 23 205 830 482
Cuts at: 4656 5754
Size: 1098
Fragments arranged by size:
1960 1098 830 748 482 361 205 47
23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 142 3871 4341 5182 5754
Size: 70 72 3729 470 841 572
Fragments arranged by size:
3729 841 572 470 72 70
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 298 1289 2481 3230 5754
Size: 13 285 991 1192 749 2524
Fragments arranged by size:
2524 1192 991 749 285 13
BsaAI yAC'GTr
Cuts at: 0 2316 5754
Size: 2316 3438
BsaHI Gr'CG_yC
Cuts at: 0 2543 2777 2789 4631 4651 5112 5754
Size: 2543 234 12 1842 20 461 642
Fragments arranged by size:
2543 1842 642 461 234 20 12
BsaWI w'CCGG_w
Cuts at: 0 50 2354 2727 3221 5754
Size: 50 2304 373 494 2533
Fragments arranged by size:
2533 2304 494 373 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 3028 3058 4030 4060 4870 4900
Size: 124 30 2874 30 972 30 810 30
Cuts at: 4900 5581 5611 5754
Size: 681 30 143
Fragments arranged by size:
2874 972 810 681 143 124 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 1927 2055 2066 2077 2080 2105 2641
Size: 19 1908 128 11 11 3 25 536
Cuts at: 2641 2746 2797 2836 2839 2848 2851 2854 2857
Size: 105 51 39 3 9 3 3 3
Cuts at: 2857 2860 2950 2992 2995 2998 3001 3004 3022
Size: 3 90 42 3 3 3 3 18
Cuts at: 3022 3025 3028 3031 3046 3049 3172 3343 3736
Size: 3 3 3 15 3 123 171 393
Cuts at: 3736 4012 4038 4162 4282 4315 4543 4738 4771
Size: 276 26 124 120 33 228 195 33
Cuts at: 4771 5224 5290 5347 5602 5605 5686 5710 5754
Size: 453 66 57 255 3 81 24 44
Fragments arranged by size:
1908 536 453 393 276 255 228 195
171 128 124 123 120 105 90 81
66 57 51 44 42 39 33 33
26 25 24 19 18 15 11 11
9 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 30 1040 1572 2032 2180 2632 3111 3511
Size: 30 1010 532 460 148 452 479 400
Cuts at: 3511 3533 3539 3799 3912 4918 5029 5754
Size: 22 6 260 113 1006 111 725
Fragments arranged by size:
1010 1006 725 532 479 460 452 400
260 148 113 111 30 22 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4021 5470 5754
Size: 4021 1449 284
Fragments arranged by size:
4021 1449 284
BsiHKAI G_wGCw'C
Cuts at: 0 104 253 614 968 2116 2750 3407 4048
Size: 104 149 361 354 1148 634 657 641
Cuts at: 4048 5549 5754
Size: 1501 205
Fragments arranged by size:
1501 1148 657 641 634 361 354 205
149 104
BsmI GAATG_Cn'
Cuts at: 0 2211 5226 5754
Size: 2211 3015 528
Fragments arranged by size:
3015 2211 528
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1061 5754
Size: 1061 4693
Bsp1286I G_dGCh'C
Cuts at: 0 104 253 606 614 968 1300 2116 2245
Size: 104 149 353 8 354 332 816 129
Cuts at: 2245 2633 2659 2750 3112 3284 3407 3472 3767
Size: 388 26 91 362 172 123 65 295
Cuts at: 3767 3794 3800 4048 4154 4190 4720 4730 4792
Size: 27 6 248 106 36 530 10 62
Cuts at: 4792 4822 5301 5549 5754
Size: 30 479 248 205
Fragments arranged by size:
816 530 479 388 362 354 353 332
295 248 248 205 172 149 129 123
106 104 91 65 62 36 30 27
26 10 8 6
BspHI T'CATG_A
Cuts at: 0 1784 5754
Size: 1784 3970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 79 5316 5754
Size: 79 5237 438
Fragments arranged by size:
5237 438 79
BsrBI CCG'CTC
Cuts at: 0 140 2667 5754
Size: 140 2527 3087
Fragments arranged by size:
3087 2527 140
BsrDI GCAATG_nn'
Cuts at: 0 1548 1751 5754
Size: 1548 203 4003
Fragments arranged by size:
4003 1548 203
BsrFI r'CCGG_y
Cuts at: 0 50 2490 4682 4901 5522 5754
Size: 50 2440 2192 219 621 232
Fragments arranged by size:
2440 2192 621 232 219 50
BstAPI GCAn_nnn'nTGC
Cuts at: 0 921 1050 5076 5754
Size: 921 129 4026 678
Fragments arranged by size:
4026 921 678 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2577 3624 3912 4521 4932 5754
Size: 2577 1047 288 609 411 822
Fragments arranged by size:
2577 1047 822 609 411 288
BstYI r'GATC_y
Cuts at: 0 1623 1942 2903 3390 3746 5754
Size: 1623 319 961 487 356 2008
Fragments arranged by size:
2008 1623 961 487 356 319
Bsu36I CC'TnA_GG
Cuts at: 0 569 2698 3326 4626 5754
Size: 569 2129 628 1300 1128
Fragments arranged by size:
2129 1300 1128 628 569
BtgI C'CryG_G
Cuts at: 0 711 1808 1832 2576 2884 3488 4505 4583
Size: 711 1097 24 744 308 604 1017 78
Cuts at: 4583 5051 5465 5754
Size: 468 414 289
Fragments arranged by size:
1097 1017 744 711 604 468 414 308
289 78 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 711 2622 5754
Size: 711 1911 3132
Fragments arranged by size:
3132 1911 711
BtsI GCAGTG_nn'
Cuts at: 0 2501 2713 5570 5754
Size: 2501 212 2857 184
Fragments arranged by size:
2857 2501 212 184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 831 866 5754
Size: 831 35 4888
Fragments arranged by size:
4888 831 35
DraIII CAC_nnn'GTG
Cuts at: 0 263 5076 5754
Size: 263 4813 678
Fragments arranged by size:
4813 678 263
DrdI GACnn_nn'nnGTC
Cuts at: 0 55 5754
Size: 55 5699
EaeI y'GGCC_r
Cuts at: 0 388 722 1097 1213 1438 1971 2189 2579
Size: 388 334 375 116 225 533 218 390
Cuts at: 2579 3481 3605 4396 4430 4523 4586 5754
Size: 902 124 791 34 93 63 1168
Fragments arranged by size:
1168 902 791 533 390 388 375 334
225 218 124 116 93 63 34
EarI CTCTTCn'nnn_
Cuts at: 0 776 2032 2195 2479 2927 2969 2999 3035
Size: 776 1256 163 284 448 42 30 36
Cuts at: 3035 3248 3730 4477 4555 4825 4834 4858 5597
Size: 213 482 747 78 270 9 24 739
Cuts at: 5597 5754
Size: 157
Fragments arranged by size:
1256 776 747 739 482 448 284 270
213 163 157 78 42 36 30 24
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3739 4086 5754
Size: 3739 347 1668
Fragments arranged by size:
3739 1668 347
EcoICRI GAG'CTC
Cuts at: 0 102 966 4046 5547 5754
Size: 102 864 3080 1501 207
Fragments arranged by size:
3080 1501 864 207 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1362 2644 2698 3154 3804 4101 4480
Size: 473 889 1282 54 456 650 297 379
Cuts at: 4480 4756 4806 5025 5130 5371 5530 5650 5689
Size: 276 50 219 105 241 159 120 39
Cuts at: 5689 5721 5731 5754
Size: 32 10 23
Fragments arranged by size:
1282 889 650 473 456 379 297 276
241 219 159 120 105 54 50 39
32 23 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2701 3718 4164 4432 5754
Size: 2701 1017 446 268 1322
Fragments arranged by size:
2701 1322 1017 446 268
EcoO109I rG'GnC_Cy
Cuts at: 0 2527 3196 3280 3281 3696 3790 4640 4761
Size: 2527 669 84 1 415 94 850 121
Cuts at: 4761 4788 5706 5754
Size: 27 918 48
Fragments arranged by size:
2527 918 850 669 415 121 94 84
48 27 1
EcoRI G'AATT_C
Cuts at: 0 544 706 1512 5754
Size: 544 162 806 4242
Fragments arranged by size:
4242 806 544 162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4992 5593 5754
Size: 4992 601 161
Fragments arranged by size:
4992 601 161
FseI GG_CCGG'CC
Cuts at: 0 4905 5754
Size: 4905 849
HaeII r_GCGC'y
Cuts at: 0 2546 2780 3336 3992 4573 4634 4683 5115
Size: 2546 234 556 656 581 61 49 432
Cuts at: 5115 5383 5665 5754
Size: 268 282 89
Fragments arranged by size:
2546 656 581 556 432 282 268 234
89 61 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 346 378 1813 1845 1934 1966
Size: 124 32 190 32 1435 32 89 32
Cuts at: 1966 1968 2000 3028 3060 3061 3093 3370 3402
Size: 2 32 1028 32 1 32 277 32
Cuts at: 3402 3928 3960 4870 4902 5005 5037 5288 5320
Size: 526 32 910 32 103 32 251 32
Cuts at: 5320 5581 5613 5754
Size: 261 32 141
Fragments arranged by size:
1435 1028 910 526 277 261 251 190
141 124 103 89 32 32 32 32
32 32 32 32 32 32 32 32
32 2 1
HincII GTy'rAC
Cuts at: 0 1428 5533 5754
Size: 1428 4105 221
Fragments arranged by size:
4105 1428 221
HindIII A'AGCT_T
Cuts at: 0 166 5754
Size: 166 5588
KasI G'GCGC_C
Cuts at: 0 2542 2776 4630 5111 5754
Size: 2542 234 1854 481 643
Fragments arranged by size:
2542 1854 643 481 234
KpnI G_GTAC'C
Cuts at: 0 2728 5754
Size: 2728 3026
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 652 679 3059 3128 3995 4076 4757
Size: 510 142 27 2380 69 867 81 681
Cuts at: 4757 4949 5462 5754
Size: 192 513 292
Fragments arranged by size:
2380 867 681 513 510 292 192 142
81 69 27
MscI TGG'CCA
Cuts at: 0 390 724 1099 1215 1973 2191 2581 4432
Size: 390 334 375 116 758 218 390 1851
Cuts at: 4432 4588 5754
Size: 156 1166
Fragments arranged by size:
1851 1166 758 390 390 375 334 218
156 116
MslI CAynn'nnrTG
Cuts at: 0 536 1117 1460 1837 3772 4228 5563 5754
Size: 536 581 343 377 1935 456 1335 191
Fragments arranged by size:
1935 1335 581 536 456 377 343 191
MspA1I CmG'CkG
Cuts at: 0 1050 2184 3115 4507 4596 5045 5118 5313
Size: 1050 1134 931 1392 89 449 73 195
Cuts at: 5313 5319 5343 5679 5754
Size: 6 24 336 75
Fragments arranged by size:
1392 1134 1050 931 449 336 195 89
75 73 24 6
NaeI GCC'GGC
Cuts at: 0 4684 4903 5754
Size: 4684 219 851
Fragments arranged by size:
4684 851 219
NarI GG'CG_CC
Cuts at: 0 2543 2777 4631 5112 5754
Size: 2543 234 1854 481 642
Fragments arranged by size:
2543 1854 642 481 234
NcoI C'CATG_G
Cuts at: 0 1832 4583 5465 5754
Size: 1832 2751 882 289
Fragments arranged by size:
2751 1832 882 289
NgoMIV G'CCGG_C
Cuts at: 0 4682 4901 5754
Size: 4682 219 853
Fragments arranged by size:
4682 853 219
NspI r_CATG'y
Cuts at: 0 2291 4539 5754
Size: 2291 2248 1215
Fragments arranged by size:
2291 2248 1215
PasI CC'CwG_GG
Cuts at: 0 4668 5754
Size: 4668 1086
PflMI CCAn_nnn'nTGG
Cuts at: 0 1637 2187 5754
Size: 1637 550 3567
Fragments arranged by size:
3567 1637 550
PfoI T'CCnGG_A
Cuts at: 0 95 824 1636 4931 5549 5684 5754
Size: 95 729 812 3295 618 135 70
Fragments arranged by size:
3295 812 729 618 135 95 70
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2329 2361 5754
Size: 2329 32 3393
Fragments arranged by size:
3393 2329 32
PpuMI rG'GwC_Cy
Cuts at: 0 2527 3196 4761 5706 5754
Size: 2527 669 1565 945 48
Fragments arranged by size:
2527 1565 945 669 48
PshAI GACnn'nnGTC
Cuts at: 0 3454 5754
Size: 3454 2300
PspOMI G'GGCC_C
Cuts at: 0 3280 3790 4788 5754
Size: 3280 510 998 966
Fragments arranged by size:
3280 998 966 510
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 853 885 5754
Size: 853 32 4869
Fragments arranged by size:
4869 853 32
PstI C_TGCA'G
Cuts at: 0 2537 2720 4367 4475 5105 5324 5540 5754
Size: 2537 183 1647 108 630 219 216 214
Fragments arranged by size:
2537 1647 630 219 216 214 183 108
PvuII CAG'CTG
Cuts at: 0 1050 2184 3115 4596 5118 5319 5754
Size: 1050 1134 931 1481 522 201 435
Fragments arranged by size:
1481 1134 1050 931 522 435 201
SacI G_AGCT'C
Cuts at: 0 104 968 4048 5549 5754
Size: 104 864 3080 1501 205
Fragments arranged by size:
3080 1501 864 205 104
SacII CC_GC'GG
Cuts at: 0 4508 5754
Size: 4508 1246
SalI G'TCGA_C
Cuts at: 0 5531 5754
Size: 5531 223
SapI GCTCTTCn'nnn_
Cuts at: 0 3248 4555 4825 5754
Size: 3248 1307 270 929
Fragments arranged by size:
3248 1307 929 270
SbfI CC_TGCA'GG
Cuts at: 0 2537 4367 5754
Size: 2537 1830 1387
Fragments arranged by size:
2537 1830 1387
ScaI AGT'ACT
Cuts at: 0 2377 5754
Size: 2377 3377
SexAI A'CCwGG_T
Cuts at: 0 3713 5754
Size: 3713 2041
SfcI C'TryA_G
Cuts at: 0 1550 2533 2716 4363 4471 5101 5320 5536
Size: 1550 983 183 1647 108 630 219 216
Cuts at: 5536 5754
Size: 218
Fragments arranged by size:
1647 1550 983 630 219 218 216 183
108
SfoI GGC'GCC
Cuts at: 0 2544 2778 4632 5113 5754
Size: 2544 234 1854 481 641
Fragments arranged by size:
2544 1854 641 481 234
SmaI CCC'GGG
Cuts at: 0 1237 4142 4243 5503 5754
Size: 1237 2905 101 1260 251
Fragments arranged by size:
2905 1260 1237 251 101
SmlI C'TyrA_G
Cuts at: 0 85 157 3886 4356 5161 5281 5578 5754
Size: 85 72 3729 470 805 120 297 176
Fragments arranged by size:
3729 805 470 297 176 120 85 72
SpeI A'CTAG_T
Cuts at: 0 1173 5754
Size: 1173 4581
SphI G_CATG'C
Cuts at: 0 4539 5754
Size: 4539 1215
StuI AGG'CCT
Cuts at: 0 2947 5754
Size: 2947 2807
StyI C'CwwG_G
Cuts at: 0 329 516 549 683 1142 1832 1895 2000
Size: 329 187 33 134 459 690 63 105
Cuts at: 2000 3659 4433 4496 4514 4583 5465 5729 5754
Size: 1659 774 63 18 69 882 264 25
Fragments arranged by size:
1659 882 774 690 459 329 264 187
134 105 69 63 63 33 25 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 409 2115 2863 3325 3525 5754
Size: 409 1706 748 462 200 2229
Fragments arranged by size:
2229 1706 748 462 409 200
TatI w'GTAC_w
Cuts at: 0 1385 2168 2375 5754
Size: 1385 783 207 3379
Fragments arranged by size:
3379 1385 783 207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1395 1427 3723 3755 4030 4062 5754
Size: 1395 32 2296 32 275 32 1692
Fragments arranged by size:
2296 1692 1395 275 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1399 5155 5754
Size: 1399 3756 599
Fragments arranged by size:
3756 1399 599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3666 4197 5754
Size: 3666 531 1557
Fragments arranged by size:
3666 1557 531
XmaI C'CCGG_G
Cuts at: 0 1235 4140 4241 5501 5754
Size: 1235 2905 101 1260 253
Fragments arranged by size:
2905 1260 1235 253 101
XmnI GAAnn'nnTTC
Cuts at: 0 692 706 2257 4375 5754
Size: 692 14 1551 2118 1379
Fragments arranged by size:
2118 1551 1379 692 14
ZraI GAC'GTC
Cuts at: 0 2790 5754
Size: 2790 2964
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII
Hin4I HincII HindIII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PpiI PpuMI PshAI PspOMI PsrI PstI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflIII AlfI AloI AscI AseI AsiSI
BaeI BfrBI BmtI BsaBI BsiEI BsiWI BspEI BsrGI
BssHII BssSI BstBI BstEII BstZ17I ClaI DraI EagI
EcoRV FspI FspAI HpaI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PsiI
PspXI PvuI RsrII SanDI SfiI SgfI SgrAI SnaBI
SrfI SspI SwaI XbaI XhoI