Restriction map for
pF1KSDA0823
(Linear) (Six-base) MAPSORT of: KIAA0823.seq Check: 4252 from: 1 to: 1701
KIAA0823.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 436 1701
Size: 436 1265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 283 1468 1701
Size: 121 162 1185 233
Fragments arranged by size:
1185 233 162 121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 444 641 821 1130 1478 1701
Size: 444 197 180 309 348 223
Fragments arranged by size:
444 348 309 223 197 180
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 901 933 1701
Size: 901 32 768
Fragments arranged by size:
901 768 32
AlwNI CAG_nnn'CTG
Cuts at: 0 39 99 663 1701
Size: 39 60 564 1038
Fragments arranged by size:
1038 564 60 39
ApaI G_GGCC'C
Cuts at: 0 93 1701
Size: 93 1608
ApoI r'AATT_y
Cuts at: 0 1228 1701
Size: 1228 473
BanI G'GyrC_C
Cuts at: 0 48 370 685 1347 1701
Size: 48 322 315 662 354
Fragments arranged by size:
662 354 322 315 48
BanII G_rGCy'C
Cuts at: 0 93 740 794 1290 1429 1701
Size: 93 647 54 496 139 272
Fragments arranged by size:
647 496 272 139 93 54
BbvCI CC'TCA_GC
Cuts at: 0 229 976 1121 1701
Size: 229 747 145 580
Fragments arranged by size:
747 580 229 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 463 497 1701
Size: 463 34 1204
Fragments arranged by size:
1204 463 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 712 1701
Size: 712 989
BclI T'GATC_A
Cuts at: 0 959 1701
Size: 959 742
BglI GCCn_nnn'nGGC
Cuts at: 0 208 1701
Size: 208 1493
BlpI GC'TnA_GC
Cuts at: 0 734 1477 1701
Size: 734 743 224
Fragments arranged by size:
743 734 224
Bme1580I G_kGCm'C
Cuts at: 0 53 93 114 1701
Size: 53 40 21 1587
Fragments arranged by size:
1587 53 40 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 786 1701
Size: 786 915
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 877 1701
Size: 877 824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 79 238 728 955 993 1225 1425
Size: 61 18 159 490 227 38 232 200
Cuts at: 1425 1462 1701
Size: 37 239
Fragments arranged by size:
490 239 232 227 200 159 61 38
37 18
Bpu10I CC'TnA_GC
Cuts at: 0 229 611 976 1121 1492 1701
Size: 229 382 365 145 371 209
Fragments arranged by size:
382 371 365 229 209 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1014 1701
Size: 1014 687
BsaI GGTCTCn'nnnn_
Cuts at: 0 544 1156 1701
Size: 544 612 545
Fragments arranged by size:
612 545 544
BsaAI yAC'GTr
Cuts at: 0 12 1701
Size: 12 1689
BsaHI Gr'CG_yC
Cuts at: 0 239 1391 1701
Size: 239 1152 310
Fragments arranged by size:
1152 310 239
BsaWI w'CCGG_w
Cuts at: 0 1290 1701
Size: 1290 411
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 746 776 901 931 1701
Size: 746 30 125 30 770
Fragments arranged by size:
770 746 125 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 730 922 1675 1684 1701
Size: 349 381 192 753 9 17
Fragments arranged by size:
753 381 349 192 17 9
BseYI C'CCAG_C
Cuts at: 0 92 785 1701
Size: 92 693 916
Fragments arranged by size:
916 693 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 1136 1701
Size: 682 454 565
Fragments arranged by size:
682 565 454
BsiEI CG_ry'CG
Cuts at: 0 177 1701
Size: 177 1524
BsiHKAI G_wGCw'C
Cuts at: 0 740 1701
Size: 740 961
Bsp1286I G_dGCh'C
Cuts at: 0 53 93 114 740 794 1290 1429 1701
Size: 53 40 21 626 54 496 139 272
Fragments arranged by size:
626 496 272 139 54 53 40 21
BspHI T'CATG_A
Cuts at: 0 962 1701
Size: 962 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 436 562 731 1701
Size: 26 410 126 169 970
Fragments arranged by size:
970 410 169 126 26
BsrBI CCG'CTC
Cuts at: 0 63 156 1701
Size: 63 93 1545
Fragments arranged by size:
1545 93 63
BsrDI GCAATG_nn'
Cuts at: 0 297 1596 1701
Size: 297 1299 105
Fragments arranged by size:
1299 297 105
BssSI C'ACGA_G
Cuts at: 0 1247 1701
Size: 1247 454
BstAPI GCAn_nnn'nTGC
Cuts at: 0 129 1347 1701
Size: 129 1218 354
Fragments arranged by size:
1218 354 129
BstEII G'GTnAC_C
Cuts at: 0 1614 1701
Size: 1614 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 1701
Size: 12 1689
BstYI r'GATC_y
Cuts at: 0 452 1241 1626 1701
Size: 452 789 385 75
Fragments arranged by size:
789 452 385 75
BtgI C'CryG_G
Cuts at: 0 366 750 849 878 1023 1701
Size: 366 384 99 29 145 678
Fragments arranged by size:
678 384 366 145 99 29
BtsI GCAGTG_nn'
Cuts at: 0 636 691 1545 1701
Size: 636 55 854 156
Fragments arranged by size:
854 636 156 55
DraIII CAC_nnn'GTG
Cuts at: 0 1252 1701
Size: 1252 449
EaeI y'GGCC_r
Cuts at: 0 2 174 432 1316 1385 1517 1701
Size: 2 172 258 884 69 132 184
Fragments arranged by size:
884 258 184 172 132 69 2
EagI C'GGCC_G
Cuts at: 0 174 1701
Size: 174 1527
EarI CTCTTCn'nnn_
Cuts at: 0 908 1701
Size: 908 793
EcoICRI GAG'CTC
Cuts at: 0 738 1701
Size: 738 963
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 79 121 238 283 728 955 993
Size: 61 18 42 117 45 445 227 38
Cuts at: 993 1225 1425 1462 1468 1701
Size: 232 200 37 6 233
Fragments arranged by size:
445 233 232 227 200 117 61 45
42 38 37 18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 217 228 520 904 1701
Size: 217 11 292 384 797
Fragments arranged by size:
797 384 292 217 11
EcoO109I rG'GnC_Cy
Cuts at: 0 660 924 1004 1188 1551 1595 1650 1701
Size: 660 264 80 184 363 44 55 51
Fragments arranged by size:
660 363 264 184 80 55 51 44
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1008 1572 1701
Size: 1008 564 129
Fragments arranged by size:
1008 564 129
HaeII r_GCGC'y
Cuts at: 0 87 1701
Size: 87 1614
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1082 1114 1701
Size: 1082 32 587
Fragments arranged by size:
1082 587 32
HincII GTy'rAC
Cuts at: 0 489 1701
Size: 489 1212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 1701
Size: 1034 667
MscI TGG'CCA
Cuts at: 0 4 1318 1519 1701
Size: 4 1314 201 182
Fragments arranged by size:
1314 201 182 4
MslI CAynn'nnrTG
Cuts at: 0 950 1701
Size: 950 751
MspA1I CmG'CkG
Cuts at: 0 36 99 652 733 805 975 1110 1120
Size: 36 63 553 81 72 170 135 10
Cuts at: 1120 1131 1458 1622 1701
Size: 11 327 164 79
Fragments arranged by size:
553 327 170 164 135 81 79 72
63 36 11 10
NcoI C'CATG_G
Cuts at: 0 366 750 849 878 1701
Size: 366 384 99 29 823
Fragments arranged by size:
823 384 366 99 29
NdeI CA'TA_TG
Cuts at: 0 510 1701
Size: 510 1191
NotI GC'GGCC_GC
Cuts at: 0 174 1701
Size: 174 1527
NspI r_CATG'y
Cuts at: 0 507 802 1266 1701
Size: 507 295 464 435
Fragments arranged by size:
507 464 435 295
PasI CC'CwG_GG
Cuts at: 0 681 1701
Size: 681 1020
PflMI CCAn_nnn'nTGG
Cuts at: 0 663 1103 1701
Size: 663 440 598
Fragments arranged by size:
663 598 440
PfoI T'CCnGG_A
Cuts at: 0 743 1701
Size: 743 958
PmlI CAC'GTG
Cuts at: 0 12 1701
Size: 12 1689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 901 933 1701
Size: 901 32 768
Fragments arranged by size:
901 768 32
PpuMI rG'GwC_Cy
Cuts at: 0 660 924 1004 1188 1595 1701
Size: 660 264 80 184 407 106
Fragments arranged by size:
660 407 264 184 106 80
PspOMI G'GGCC_C
Cuts at: 0 89 1701
Size: 89 1612
PstI C_TGCA'G
Cuts at: 0 35 423 1467 1701
Size: 35 388 1044 234
Fragments arranged by size:
1044 388 234 35
PvuII CAG'CTG
Cuts at: 0 36 99 733 805 975 1120 1701
Size: 36 63 634 72 170 145 581
Fragments arranged by size:
634 581 170 145 72 63 36
SacI G_AGCT'C
Cuts at: 0 740 1701
Size: 740 961
SexAI A'CCwGG_T
Cuts at: 0 443 1701
Size: 443 1258
SfcI C'TryA_G
Cuts at: 0 31 419 1356 1463 1701
Size: 31 388 937 107 238
Fragments arranged by size:
937 388 238 107 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 208 1701
Size: 208 1493
SmlI C'TyrA_G
Cuts at: 0 993 1701
Size: 993 708
SphI G_CATG'C
Cuts at: 0 802 1701
Size: 802 899
StuI AGG'CCT
Cuts at: 0 223 1701
Size: 223 1478
StyI C'CwwG_G
Cuts at: 0 366 386 750 849 878 1701
Size: 366 20 364 99 29 823
Fragments arranged by size:
823 366 364 99 29 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 592 1701
Size: 592 1109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 746 778 973 1005 1701
Size: 746 32 195 32 696
Fragments arranged by size:
746 696 195 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1662 1701
Size: 1662 39
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApaI ApoI BanI
BanII BbvCI BcgI BciVI BclI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BssSI BstAPI BstEII BstXI
BstYI BtgI BtsI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NcoI NdeI NotI NspI PasI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SexAI SfcI SfiI SmlI SphI StuI StyI
TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BfrBI BglII BmgBI BmtI
BsaBI BsiWI BsmI BsmBI BspEI BsrFI BsrGI BssHII
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EciI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NruI NsiI PacI PciI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TatI Tth111I XbaI XhoI XmaI XmnI
ZraI