Restriction map for
pF1KSDA0827
(Linear) (Six-base) MAPSORT of: KIAA0827.seq Check: 1906 from: 1 to: 4593
KIAA0827.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 2827 4593
Size: 2827 1766
Acc65I G'GTAC_C
Cuts at: 0 588 884 3109 4593
Size: 588 296 2225 1484
Fragments arranged by size:
2225 1484 588 296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 531 865 2296 2411 2660 2744 2761
Size: 191 340 334 1431 115 249 84 17
Cuts at: 2761 3371 3433 3493 3991 4593
Size: 610 62 60 498 602
Fragments arranged by size:
1431 610 602 498 340 334 249 191
115 84 62 60 17
AflIII A'CryG_T
Cuts at: 0 2621 4593
Size: 2621 1972
AhdI GACnn_n'nnGTC
Cuts at: 0 3405 4593
Size: 3405 1188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 322 1710 2495 4593
Size: 322 1388 785 2098
Fragments arranged by size:
2098 1388 785 322
AlwNI CAG_nnn'CTG
Cuts at: 0 1474 1642 2077 2331 2884 4593
Size: 1474 168 435 254 553 1709
Fragments arranged by size:
1709 1474 553 435 254 168
ApoI r'AATT_y
Cuts at: 0 2507 3174 4220 4302 4491 4593
Size: 2507 667 1046 82 189 102
Fragments arranged by size:
2507 1046 667 189 102 82
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 572 605 2168 2201 4072 4105 4593
Size: 572 33 1563 33 1871 33 488
Fragments arranged by size:
1871 1563 572 488 33 33 33
BanI G'GyrC_C
Cuts at: 0 588 884 3109 4593
Size: 588 296 2225 1484
Fragments arranged by size:
2225 1484 588 296
BanII G_rGCy'C
Cuts at: 0 799 882 3385 3649 4593
Size: 799 83 2503 264 944
Fragments arranged by size:
2503 944 799 264 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 3182 4593
Size: 3182 1411
BbvCI CC'TCA_GC
Cuts at: 0 327 4593
Size: 327 4266
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 862 896 4593
Size: 862 34 3697
Fragments arranged by size:
3697 862 34
BclI T'GATC_A
Cuts at: 0 4401 4593
Size: 4401 192
BglII A'GATC_T
Cuts at: 0 1594 1849 4593
Size: 1594 255 2744
Fragments arranged by size:
2744 1594 255
BlpI GC'TnA_GC
Cuts at: 0 25 4593
Size: 25 4568
BmrI ACTGGGnnnn_n'
Cuts at: 0 4020 4593
Size: 4020 573
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 186 339 3749 4593
Size: 186 153 3410 844
Fragments arranged by size:
3410 844 186 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 803 1610 2429 4115 4593
Size: 164 639 807 819 1686 478
Fragments arranged by size:
1686 819 807 639 478 164
Bpu10I CC'TnA_GC
Cuts at: 0 327 4593
Size: 327 4266
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3041 3248 3380 4085 4593
Size: 3041 207 132 705 508
Fragments arranged by size:
3041 705 508 207 132
BsaI GGTCTCn'nnnn_
Cuts at: 0 2011 4593
Size: 2011 2582
BsaBI GATnn'nnATC
Cuts at: 0 498 4593
Size: 498 4095
BsaHI Gr'CG_yC
Cuts at: 0 3838 4593
Size: 3838 755
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 1608 1638 4593
Size: 91 30 1487 30 2955
Fragments arranged by size:
2955 1487 91 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 167 207 315 2861 3312 4055 4178 4593
Size: 167 40 108 2546 451 743 123 415
Fragments arranged by size:
2546 743 451 415 167 123 108 40
BseYI C'CCAG_C
Cuts at: 0 1632 4593
Size: 1632 2961
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2501 4593
Size: 2501 2092
BsiHKAI G_wGCw'C
Cuts at: 0 562 882 4593
Size: 562 320 3711
Fragments arranged by size:
3711 562 320
BsmI GAATG_Cn'
Cuts at: 0 1179 4349 4593
Size: 1179 3170 244
Fragments arranged by size:
3170 1179 244
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3845 4593
Size: 3845 748
Bsp1286I G_dGCh'C
Cuts at: 0 562 799 882 3385 3649 4593
Size: 562 237 83 2503 264 944
Fragments arranged by size:
2503 944 562 264 237 83
BspHI T'CATG_A
Cuts at: 0 1256 1533 1599 4593
Size: 1256 277 66 2994
Fragments arranged by size:
2994 1256 277 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2907 4593
Size: 2907 1686
BsrDI GCAATG_nn'
Cuts at: 0 19 1727 2690 3365 4393 4555 4593
Size: 19 1708 963 675 1028 162 38
Fragments arranged by size:
1708 1028 963 675 162 38 19
BsrGI T'GTAC_A
Cuts at: 0 425 855 4593
Size: 425 430 3738
Fragments arranged by size:
3738 430 425
BssSI C'ACGA_G
Cuts at: 0 1226 4593
Size: 1226 3367
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1645 2077 3481 4593
Size: 1645 432 1404 1112
Fragments arranged by size:
1645 1404 1112 432
BstEII G'GTnAC_C
Cuts at: 0 4088 4593
Size: 4088 505
BstYI r'GATC_y
Cuts at: 0 1594 1849 4593
Size: 1594 255 2744
Fragments arranged by size:
2744 1594 255
Bsu36I CC'TnA_GG
Cuts at: 0 443 3632 4593
Size: 443 3189 961
Fragments arranged by size:
3189 961 443
BtgI C'CryG_G
Cuts at: 0 227 332 722 903 3020 3824 4593
Size: 227 105 390 181 2117 804 769
Fragments arranged by size:
2117 804 769 390 227 181 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2117 4593
Size: 2117 2476
BtsI GCAGTG_nn'
Cuts at: 0 1282 2379 2857 4593
Size: 1282 1097 478 1736
Fragments arranged by size:
1736 1282 1097 478
DraIII CAC_nnn'GTG
Cuts at: 0 705 4593
Size: 705 3888
DrdI GACnn_nn'nnGTC
Cuts at: 0 1875 4593
Size: 1875 2718
EaeI y'GGCC_r
Cuts at: 0 4412 4593
Size: 4412 181
EarI CTCTTCn'nnn_
Cuts at: 0 225 264 403 787 1302 1700 2583 3390
Size: 225 39 139 384 515 398 883 807
Cuts at: 3390 4126 4593
Size: 736 467
Fragments arranged by size:
883 807 736 515 467 398 384 225
139 39
EcoICRI GAG'CTC
Cuts at: 0 880 4593
Size: 880 3713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 191 531 803 865 1610 2296 2411
Size: 164 27 340 272 62 745 686 115
Cuts at: 2411 2429 2660 2744 2761 3371 3433 3493 3991
Size: 18 231 84 17 610 62 60 498
Cuts at: 3991 4115 4593
Size: 124 478
Fragments arranged by size:
745 686 610 498 478 340 272 231
164 124 115 84 62 62 60 27
18 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 892 3253 4593
Size: 892 2361 1340
Fragments arranged by size:
2361 1340 892
EcoO109I rG'GnC_Cy
Cuts at: 0 4018 4593
Size: 4018 575
EcoRI G'AATT_C
Cuts at: 0 4220 4302 4491 4593
Size: 4220 82 189 102
Fragments arranged by size:
4220 189 102 82
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1129 1368 2491 3082 4593
Size: 1129 239 1123 591 1511
Fragments arranged by size:
1511 1129 1123 591 239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 31 33 62 63 94 523 555
Size: 1 30 2 29 1 31 429 32
Cuts at: 555 1238 1270 1441 1473 3142 3174 3391 3423
Size: 683 32 171 32 1669 32 217 32
Cuts at: 3423 4593
Size: 1170
Fragments arranged by size:
1669 1170 683 429 217 171 32 32
32 32 32 31 30 29 2 1
1
HincII GTy'rAC
Cuts at: 0 371 2385 3063 3410 3515 4593
Size: 371 2014 678 347 105 1078
Fragments arranged by size:
2014 1078 678 371 347 105
HindIII A'AGCT_T
Cuts at: 0 1350 4593
Size: 1350 3243
HpaI GTT'AAC
Cuts at: 0 371 3063 4593
Size: 371 2692 1530
Fragments arranged by size:
2692 1530 371
KpnI G_GTAC'C
Cuts at: 0 592 888 3113 4593
Size: 592 296 2225 1480
Fragments arranged by size:
2225 1480 592 296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 566 1863 2418 3570 4010 4593
Size: 445 121 1297 555 1152 440 583
Fragments arranged by size:
1297 1152 583 555 445 440 121
MscI TGG'CCA
Cuts at: 0 4414 4593
Size: 4414 179
MslI CAynn'nnrTG
Cuts at: 0 2938 3370 4593
Size: 2938 432 1223
Fragments arranged by size:
2938 1223 432
MspA1I CmG'CkG
Cuts at: 0 184 1642 2091 2331 2722 4593
Size: 184 1458 449 240 391 1871
Fragments arranged by size:
1871 1458 449 391 240 184
NcoI C'CATG_G
Cuts at: 0 227 3020 3824 4593
Size: 227 2793 804 769
Fragments arranged by size:
2793 804 769 227
NruI TCG'CGA
Cuts at: 0 69 4593
Size: 69 4524
NspI r_CATG'y
Cuts at: 0 4215 4593
Size: 4215 378
PasI CC'CwG_GG
Cuts at: 0 3642 4593
Size: 3642 951
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 549 581 4593
Size: 549 32 4012
Fragments arranged by size:
4012 549 32
PpuMI rG'GwC_Cy
Cuts at: 0 4018 4593
Size: 4018 575
PshAI GACnn'nnGTC
Cuts at: 0 3022 4593
Size: 3022 1571
PstI C_TGCA'G
Cuts at: 0 517 1042 2880 4301 4593
Size: 517 525 1838 1421 292
Fragments arranged by size:
1838 1421 525 517 292
PvuII CAG'CTG
Cuts at: 0 184 1642 2091 2331 2722 4593
Size: 184 1458 449 240 391 1871
Fragments arranged by size:
1871 1458 449 391 240 184
SacI G_AGCT'C
Cuts at: 0 882 4593
Size: 882 3711
SapI GCTCTTCn'nnn_
Cuts at: 0 787 2583 3390 4593
Size: 787 1796 807 1203
Fragments arranged by size:
1796 1203 807 787
ScaI AGT'ACT
Cuts at: 0 2358 4593
Size: 2358 2235
SfcI C'TryA_G
Cuts at: 0 131 513 941 971 1038 2876 4297 4362
Size: 131 382 428 30 67 1838 1421 65
Cuts at: 4362 4593
Size: 231
Fragments arranged by size:
1838 1421 428 382 231 131 67 65
30
SmlI C'TyrA_G
Cuts at: 0 3056 3263 3395 4100 4593
Size: 3056 207 132 705 493
Fragments arranged by size:
3056 705 493 207 132
SspI AAT'ATT
Cuts at: 0 1758 2280 4593
Size: 1758 522 2313
Fragments arranged by size:
2313 1758 522
StuI AGG'CCT
Cuts at: 0 1036 4593
Size: 1036 3557
StyI C'CwwG_G
Cuts at: 0 227 2000 3020 3824 4566 4593
Size: 227 1773 1020 804 742 27
Fragments arranged by size:
1773 1020 804 742 227 27
TatI w'GTAC_w
Cuts at: 0 425 855 1312 2356 4593
Size: 425 430 457 1044 2237
Fragments arranged by size:
2237 1044 457 430 425
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 472 504 1608 1640 4593
Size: 472 32 1104 32 2953
Fragments arranged by size:
2953 1104 472 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2389 4593
Size: 2389 2204
XbaI T'CTAG_A
Cuts at: 0 118 2152 4593
Size: 118 2034 2441
Fragments arranged by size:
2441 2034 118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 778 3831 4593
Size: 778 3053 762
Fragments arranged by size:
3053 778 762
XmnI GAAnn'nnTTC
Cuts at: 0 1493 2535 2600 4593
Size: 1493 1042 65 1993
Fragments arranged by size:
1993 1493 1042 65
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI AlfI AlwNI ApoI
BaeI BanI BanII BbsI BbvCI BcgI BclI BglII
BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I
NcoI NruI NspI PasI PpiI PpuMI PshAI PstI
PvuII SacI SapI ScaI SfcI SmlI SspI StuI
StyI TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BciVI BfrBI BglI Bme1580I BmgBI BmtI BsaAI
BsaWI BsiEI BsiWI BspEI BsrBI BsrFI BssHII BstBI
BstXI BstZ17I ClaI CspCI DraI EagI EciI EcoRV
FseI FspI FspAI HaeII KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NsiI PacI PciI
PflMI PfoI PmeI PmlI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TaqII XhoI XmaI ZraI