Restriction map for
pF1KSDA0828
(Linear) (Six-base) MAPSORT of: KIAA0828.seq Check: 7688 from: 1 to: 1830
KIAA0828.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1736 1830
Size: 1736 94
AatII G_ACGT'C
Cuts at: 0 420 1830
Size: 420 1410
AccI GT'mk_AC
Cuts at: 0 1682 1830
Size: 1682 148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1830
Size: 76 1754
AflIII A'CryG_T
Cuts at: 0 968 1830
Size: 968 862
ApaI G_GGCC'C
Cuts at: 0 206 750 1805 1830
Size: 206 544 1055 25
Fragments arranged by size:
1055 544 206 25
ApoI r'AATT_y
Cuts at: 0 388 1096 1830
Size: 388 708 734
Fragments arranged by size:
734 708 388
AvaI C'yCGr_G
Cuts at: 0 192 198 1830
Size: 192 6 1632
Fragments arranged by size:
1632 192 6
BanI G'GyrC_C
Cuts at: 0 39 115 237 290 328 342 1830
Size: 39 76 122 53 38 14 1488
Fragments arranged by size:
1488 122 76 53 39 38 14
BanII G_rGCy'C
Cuts at: 0 70 199 206 651 750 1243 1805 1830
Size: 70 129 7 445 99 493 562 25
Fragments arranged by size:
562 493 445 129 99 70 25 7
BbeI G_GCGC'C
Cuts at: 0 119 346 1830
Size: 119 227 1484
Fragments arranged by size:
1484 227 119
BciVI GTATCCnnnnn_n'
Cuts at: 0 971 1830
Size: 971 859
BglI GCCn_nnn'nGGC
Cuts at: 0 130 326 1830
Size: 130 196 1504
Fragments arranged by size:
1504 196 130
BglII A'GATC_T
Cuts at: 0 1498 1830
Size: 1498 332
BlpI GC'TnA_GC
Cuts at: 0 468 1830
Size: 468 1362
Bme1580I G_kGCm'C
Cuts at: 0 206 750 1281 1805 1830
Size: 206 544 531 524 25
Fragments arranged by size:
544 531 524 206 25
BmgBI CAC'GTC
Cuts at: 0 1455 1830
Size: 1455 375
BmrI ACTGGGnnnn_n'
Cuts at: 0 744 1830
Size: 744 1086
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 692 1031 1830
Size: 140 552 339 799
Fragments arranged by size:
799 552 339 140
Bpu10I CC'TnA_GC
Cuts at: 0 64 1573 1830
Size: 64 1509 257
Fragments arranged by size:
1509 257 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 1487 1830
Size: 635 852 343
Fragments arranged by size:
852 635 343
BsaI GGTCTCn'nnnn_
Cuts at: 0 137 1830
Size: 137 1693
BsaHI Gr'CG_yC
Cuts at: 0 116 247 343 417 1830
Size: 116 131 96 74 1413
Fragments arranged by size:
1413 131 116 96 74
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1012 1830
Size: 1012 818
BseYI C'CCAG_C
Cuts at: 0 1042 1830
Size: 1042 788
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1830
Size: 28 1802
BsiEI CG_ry'CG
Cuts at: 0 107 1830
Size: 107 1723
BsiHKAI G_wGCw'C
Cuts at: 0 103 651 741 1391 1604 1830
Size: 103 548 90 650 213 226
Fragments arranged by size:
650 548 226 213 103 90
Bsp1286I G_dGCh'C
Cuts at: 0 70 103 199 206 651 741 750 1243
Size: 70 33 96 7 445 90 9 493
Cuts at: 1243 1281 1391 1604 1805 1830
Size: 38 110 213 201 25
Fragments arranged by size:
493 445 213 201 110 96 90 70
38 33 25 9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1736 1830
Size: 1736 94
BsrBI CCG'CTC
Cuts at: 0 174 214 1830
Size: 174 40 1616
Fragments arranged by size:
1616 174 40
BsrGI T'GTAC_A
Cuts at: 0 1357 1830
Size: 1357 473
BstEII G'GTnAC_C
Cuts at: 0 1377 1830
Size: 1377 453
BstXI CCAn_nnnn'nTGG
Cuts at: 0 684 1701 1782 1830
Size: 684 1017 81 48
Fragments arranged by size:
1017 684 81 48
BstYI r'GATC_y
Cuts at: 0 362 933 1498 1830
Size: 362 571 565 332
Fragments arranged by size:
571 565 362 332
Bsu36I CC'TnA_GG
Cuts at: 0 44 149 242 1658 1830
Size: 44 105 93 1416 172
Fragments arranged by size:
1416 172 105 93 44
BtgI C'CryG_G
Cuts at: 0 23 110 119 167 1235 1830
Size: 23 87 9 48 1068 595
Fragments arranged by size:
1068 595 87 48 23 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 491 1830
Size: 491 1339
BtsI GCAGTG_nn'
Cuts at: 0 552 701 1610 1830
Size: 552 149 909 220
Fragments arranged by size:
909 552 220 149
ClaI AT'CG_AT
Cuts at: 0 875 1830
Size: 875 955
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 393 428 1830
Size: 393 35 1402
Fragments arranged by size:
1402 393 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 331 1830
Size: 331 1499
EaeI y'GGCC_r
Cuts at: 0 104 251 1715 1830
Size: 104 147 1464 115
Fragments arranged by size:
1464 147 115 104
EagI C'GGCC_G
Cuts at: 0 104 1830
Size: 104 1726
EarI CTCTTCn'nnn_
Cuts at: 0 585 637 1830
Size: 585 52 1193
Fragments arranged by size:
1193 585 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 94 1830
Size: 94 1736
EcoICRI GAG'CTC
Cuts at: 0 649 1830
Size: 649 1181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 140 692 1031 1830
Size: 76 64 552 339 799
Fragments arranged by size:
799 552 339 76 64
EcoO109I rG'GnC_Cy
Cuts at: 0 60 202 309 746 1802 1830
Size: 60 142 107 437 1056 28
Fragments arranged by size:
1056 437 142 107 60 28
EcoRI G'AATT_C
Cuts at: 0 388 1830
Size: 388 1442
HaeII r_GCGC'y
Cuts at: 0 119 346 1830
Size: 119 227 1484
Fragments arranged by size:
1484 227 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 490 522 665 697 1830
Size: 67 32 391 32 143 32 1133
Fragments arranged by size:
1133 391 143 67 32 32 32
HincII GTy'rAC
Cuts at: 0 1509 1830
Size: 1509 321
KasI G'GCGC_C
Cuts at: 0 115 342 1830
Size: 115 227 1488
Fragments arranged by size:
1488 227 115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1356 1463 1830
Size: 1356 107 367
Fragments arranged by size:
1356 367 107
MscI TGG'CCA
Cuts at: 0 1717 1830
Size: 1717 113
MspA1I CmG'CkG
Cuts at: 0 25 169 288 472 1830
Size: 25 144 119 184 1358
Fragments arranged by size:
1358 184 144 119 25
NarI GG'CG_CC
Cuts at: 0 116 343 1830
Size: 116 227 1487
Fragments arranged by size:
1487 227 116
NcoI C'CATG_G
Cuts at: 0 119 1235 1830
Size: 119 1116 595
Fragments arranged by size:
1116 595 119
NspI r_CATG'y
Cuts at: 0 972 1830
Size: 972 858
PasI CC'CwG_GG
Cuts at: 0 512 1830
Size: 512 1318
PciI A'CATG_T
Cuts at: 0 968 1830
Size: 968 862
PpuMI rG'GwC_Cy
Cuts at: 0 60 1830
Size: 60 1770
PsiI TTA'TAA
Cuts at: 0 953 1830
Size: 953 877
PspOMI G'GGCC_C
Cuts at: 0 202 746 1801 1830
Size: 202 544 1055 29
Fragments arranged by size:
1055 544 202 29
SacI G_AGCT'C
Cuts at: 0 651 1830
Size: 651 1179
SacII CC_GC'GG
Cuts at: 0 26 170 1830
Size: 26 144 1660
Fragments arranged by size:
1660 144 26
ScaI AGT'ACT
Cuts at: 0 1544 1830
Size: 1544 286
SfcI C'TryA_G
Cuts at: 0 462 1821 1830
Size: 462 1359 9
Fragments arranged by size:
1359 462 9
SfoI GGC'GCC
Cuts at: 0 117 344 1830
Size: 117 227 1486
Fragments arranged by size:
1486 227 117
SmaI CCC'GGG
Cuts at: 0 194 1830
Size: 194 1636
SmlI C'TyrA_G
Cuts at: 0 650 1502 1830
Size: 650 852 328
Fragments arranged by size:
852 650 328
SrfI GCCC'GGGC
Cuts at: 0 194 1830
Size: 194 1636
StyI C'CwwG_G
Cuts at: 0 35 119 1235 1830
Size: 35 84 1116 595
Fragments arranged by size:
1116 595 84 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 724 1830
Size: 724 1106
TatI w'GTAC_w
Cuts at: 0 1357 1542 1830
Size: 1357 185 288
Fragments arranged by size:
1357 288 185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1038 1070 1163 1195 1830
Size: 1038 32 93 32 635
Fragments arranged by size:
1038 635 93 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1081 1830
Size: 1081 749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1389 1481 1830
Size: 1389 92 349
Fragments arranged by size:
1389 349 92
XmaI C'CCGG_G
Cuts at: 0 192 1830
Size: 192 1638
XmnI GAAnn'nnTTC
Cuts at: 0 1127 1830
Size: 1127 703
ZraI GAC'GTC
Cuts at: 0 418 1830
Size: 418 1412
Enzymes that do cut:
AarI AatII AccI AcuI AflIII ApaI ApoI AvaI
BanI BanII BbeI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrGI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI HaeII Hin4I HincII KasI MmeI MscI
MspA1I NarI NcoI NspI PasI PciI PpuMI PsiI
PspOMI SacI SacII ScaI SfcI SfoI SmaI SmlI
SrfI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BclI BfrBI BmtI BplI
BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstZ17I
DraI DraIII EcoNI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PflMI PfoI
PmeI PmlI PpiI PshAI PspXI PsrI PstI PvuI
PvuII RsrII SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI
XbaI XhoI