Restriction map for
pF1KSDA0831
(Linear) (Six-base) MAPSORT of: KIAA0831.seq Check: 4512 from: 1 to: 1137
KIAA0831.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1060 1137
Size: 1060 77
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 311 395 1137
Size: 95 216 84 742
Fragments arranged by size:
742 216 95 84
AflII C'TTAA_G
Cuts at: 0 207 1137
Size: 207 930
AhdI GACnn_n'nnGTC
Cuts at: 0 409 1137
Size: 409 728
ApaI G_GGCC'C
Cuts at: 0 842 1137
Size: 842 295
ApoI r'AATT_y
Cuts at: 0 69 658 688 753 877 1137
Size: 69 589 30 65 124 260
Fragments arranged by size:
589 260 124 69 65 30
AvaI C'yCGr_G
Cuts at: 0 695 1137
Size: 695 442
AvrII C'CTAG_G
Cuts at: 0 825 1137
Size: 825 312
BamHI G'GATC_C
Cuts at: 0 885 1137
Size: 885 252
BanI G'GyrC_C
Cuts at: 0 131 1137
Size: 131 1006
BanII G_rGCy'C
Cuts at: 0 263 842 1137
Size: 263 579 295
Fragments arranged by size:
579 295 263
BbsI GAAGACnn'nnnn_
Cuts at: 0 317 1137
Size: 317 820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1079 1113 1137
Size: 1079 34 24
Fragments arranged by size:
1079 34 24
Bme1580I G_kGCm'C
Cuts at: 0 842 958 1137
Size: 842 116 179
Fragments arranged by size:
842 179 116
BmrI ACTGGGnnnn_n'
Cuts at: 0 972 999 1137
Size: 972 27 138
Fragments arranged by size:
972 138 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 282 1137
Size: 282 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 920 1137
Size: 336 584 217
Fragments arranged by size:
584 336 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1137
Size: 883 254
BsaI GGTCTCn'nnnn_
Cuts at: 0 302 1137
Size: 302 835
BsaBI GATnn'nnATC
Cuts at: 0 629 1137
Size: 629 508
BsaHI Gr'CG_yC
Cuts at: 0 1042 1137
Size: 1042 95
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 526 880 1074 1137
Size: 526 354 194 63
Fragments arranged by size:
526 354 194 63
BseYI C'CCAG_C
Cuts at: 0 503 1137
Size: 503 634
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1088 1137
Size: 1088 49
BsiHKAI G_wGCw'C
Cuts at: 0 126 263 1036 1137
Size: 126 137 773 101
Fragments arranged by size:
773 137 126 101
BsmI GAATG_Cn'
Cuts at: 0 720 1137
Size: 720 417
Bsp1286I G_dGCh'C
Cuts at: 0 126 263 842 958 1036 1137
Size: 126 137 579 116 78 101
Fragments arranged by size:
579 137 126 116 101 78
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 821 1060 1137
Size: 821 239 77
Fragments arranged by size:
821 239 77
BsrBI CCG'CTC
Cuts at: 0 917 1137
Size: 917 220
BstEII G'GTnAC_C
Cuts at: 0 178 1101 1137
Size: 178 923 36
Fragments arranged by size:
923 178 36
BstYI r'GATC_y
Cuts at: 0 885 1137
Size: 885 252
Bsu36I CC'TnA_GG
Cuts at: 0 784 1137
Size: 784 353
BtgI C'CryG_G
Cuts at: 0 872 1137
Size: 872 265
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 950 1038 1137
Size: 950 88 99
Fragments arranged by size:
950 99 88
BtsI GCAGTG_nn'
Cuts at: 0 707 772 1071 1137
Size: 707 65 299 66
Fragments arranged by size:
707 299 66 65
DraI TTT'AAA
Cuts at: 0 1116 1137
Size: 1116 21
EcoICRI GAG'CTC
Cuts at: 0 261 1137
Size: 261 876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 311 336 395 920 1137
Size: 95 216 25 59 525 217
Fragments arranged by size:
525 217 216 95 59 25
EcoO109I rG'GnC_Cy
Cuts at: 0 454 532 838 839 1137
Size: 454 78 306 1 298
Fragments arranged by size:
454 306 298 78 1
EcoRV GAT'ATC
Cuts at: 0 996 1137
Size: 996 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 150 182 395 427 1137
Size: 150 32 213 32 710
Fragments arranged by size:
710 213 150 32 32
HincII GTy'rAC
Cuts at: 0 603 1137
Size: 603 534
HindIII A'AGCT_T
Cuts at: 0 109 367 1118 1137
Size: 109 258 751 19
Fragments arranged by size:
751 258 109 19
MspA1I CmG'CkG
Cuts at: 0 503 597 1137
Size: 503 94 540
Fragments arranged by size:
540 503 94
NcoI C'CATG_G
Cuts at: 0 872 1137
Size: 872 265
NruI TCG'CGA
Cuts at: 0 1057 1137
Size: 1057 80
NspI r_CATG'y
Cuts at: 0 751 1137
Size: 751 386
PasI CC'CwG_GG
Cuts at: 0 528 1137
Size: 528 609
PflMI CCAn_nnn'nTGG
Cuts at: 0 1011 1137
Size: 1011 126
PfoI T'CCnGG_A
Cuts at: 0 888 1137
Size: 888 249
PspOMI G'GGCC_C
Cuts at: 0 838 1137
Size: 838 299
PspXI vC'TCGA_Gb
Cuts at: 0 695 1137
Size: 695 442
PstI C_TGCA'G
Cuts at: 0 1092 1137
Size: 1092 45
PvuII CAG'CTG
Cuts at: 0 503 597 1137
Size: 503 94 540
Fragments arranged by size:
540 503 94
SacI G_AGCT'C
Cuts at: 0 263 1137
Size: 263 874
SexAI A'CCwGG_T
Cuts at: 0 182 800 1137
Size: 182 618 337
Fragments arranged by size:
618 337 182
SfcI C'TryA_G
Cuts at: 0 498 1088 1137
Size: 498 590 49
Fragments arranged by size:
590 498 49
SmlI C'TyrA_G
Cuts at: 0 207 695 862 1137
Size: 207 488 167 275
Fragments arranged by size:
488 275 207 167
SspI AAT'ATT
Cuts at: 0 723 1137
Size: 723 414
StuI AGG'CCT
Cuts at: 0 80 1137
Size: 80 1057
StyI C'CwwG_G
Cuts at: 0 92 457 825 872 1137
Size: 92 365 368 47 265
Fragments arranged by size:
368 365 265 92 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 495 1051 1065 1137
Size: 495 556 14 72
Fragments arranged by size:
556 495 72 14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 879 1137
Size: 879 258
XhoI C'TCGA_G
Cuts at: 0 695 1137
Size: 695 442
XmnI GAAnn'nnTTC
Cuts at: 0 80 1137
Size: 80 1057
Enzymes that do cut:
AarI AcuI AflII AhdI ApaI ApoI AvaI AvrII
BamHI BanI BanII BbsI BcgI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaBI BsaHI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI EcoICRI Eco57MI EcoO109I EcoRV
Hin4I HincII HindIII MspA1I NcoI NruI NspI PasI
PflMI PfoI PspOMI PspXI PstI PvuII SacI SexAI
SfcI SmlI SspI StuI StyI TaqII XcmI XhoI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI BaeI
BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI Bpu10I BsaAI BsaWI BsaXI BsiEI BsiWI
BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstZ17I ClaI CspCI DraIII DrdI
EaeI EagI EarI EciI EcoNI EcoRI FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI NaeI NarI NdeI NgoMIV NheI
NotI NsiI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI TatI TstI Tth111I
XbaI XmaI ZraI