Restriction map for
pF1KA0835
(Linear) (Six-base) MAPSORT of: KIAA0835.seq Check: 1825 from: 1 to: 3363
KIAA0835.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 596 3363
Size: 596 2767
AatII G_ACGT'C
Cuts at: 0 358 3363
Size: 358 3005
AccI GT'mk_AC
Cuts at: 0 1988 3363
Size: 1988 1375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 413 545 577 1180 1471 2294 2377
Size: 256 157 132 32 603 291 823 83
Cuts at: 2377 2836 3136 3363
Size: 459 300 227
Fragments arranged by size:
823 603 459 300 291 256 227 157
132 83 32
AfeI AGC'GCT
Cuts at: 0 691 3363
Size: 691 2672
AflIII A'CryG_T
Cuts at: 0 2987 3287 3363
Size: 2987 300 76
Fragments arranged by size:
2987 300 76
AleI CACnn'nnGTG
Cuts at: 0 292 3363
Size: 292 3071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1810 2763 3363
Size: 1810 953 600
Fragments arranged by size:
1810 953 600
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2431 2463 3363
Size: 2431 32 900
Fragments arranged by size:
2431 900 32
AlwNI CAG_nnn'CTG
Cuts at: 0 574 702 717 925 1090 2063 3363
Size: 574 128 15 208 165 973 1300
Fragments arranged by size:
1300 973 574 208 165 128 15
ApaI G_GGCC'C
Cuts at: 0 991 3363
Size: 991 2372
ApaLI G'TGCA_C
Cuts at: 0 583 3363
Size: 583 2780
ApoI r'AATT_y
Cuts at: 0 2304 3255 3363
Size: 2304 951 108
Fragments arranged by size:
2304 951 108
AvaI C'yCGr_G
Cuts at: 0 382 1121 1654 2662 2844 3363
Size: 382 739 533 1008 182 519
Fragments arranged by size:
1008 739 533 519 382 182
BamHI G'GATC_C
Cuts at: 0 424 1412 1622 3363
Size: 424 988 210 1741
Fragments arranged by size:
1741 988 424 210
BanI G'GyrC_C
Cuts at: 0 140 2634 3363
Size: 140 2494 729
Fragments arranged by size:
2494 729 140
BanII G_rGCy'C
Cuts at: 0 30 406 983 991 2702 3363
Size: 30 376 577 8 1711 661
Fragments arranged by size:
1711 661 577 376 30 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 23 950 3363
Size: 23 927 2413
Fragments arranged by size:
2413 927 23
BbvCI CC'TCA_GC
Cuts at: 0 82 194 1939 3363
Size: 82 112 1745 1424
Fragments arranged by size:
1745 1424 112 82
BciVI GTATCCnnnnn_n'
Cuts at: 0 2333 3363
Size: 2333 1030
BfrBI ATG'CAT
Cuts at: 0 1437 3363
Size: 1437 1926
BglI GCCn_nnn'nGGC
Cuts at: 0 970 1162 1223 1391 1717 3363
Size: 970 192 61 168 326 1646
Fragments arranged by size:
1646 970 326 192 168 61
BglII A'GATC_T
Cuts at: 0 1424 3092 3363
Size: 1424 1668 271
Fragments arranged by size:
1668 1424 271
BlpI GC'TnA_GC
Cuts at: 0 200 3363
Size: 200 3163
Bme1580I G_kGCm'C
Cuts at: 0 119 587 991 1459 2533 2680 3363
Size: 119 468 404 468 1074 147 683
Fragments arranged by size:
1074 683 468 468 404 147 119
BmgBI CAC'GTC
Cuts at: 0 120 2988 3363
Size: 120 2868 375
Fragments arranged by size:
2868 375 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 705 995 2145 2674 3363
Size: 705 290 1150 529 689
Fragments arranged by size:
1150 705 689 529 290
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1056 1755 3069 3363
Size: 1056 699 1314 294
Fragments arranged by size:
1314 1056 699 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 205 355 739 1162 1750 3193 3346
Size: 120 85 150 384 423 588 1443 153
Cuts at: 3346 3363
Size: 17
Fragments arranged by size:
1443 588 423 384 153 150 120 85
17
Bpu10I CC'TnA_GC
Cuts at: 0 82 111 194 977 1775 1939 3187 3363
Size: 82 29 83 783 798 164 1248 176
Fragments arranged by size:
1248 798 783 176 164 83 82 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2031 2509 3363
Size: 2031 478 854
Fragments arranged by size:
2031 854 478
BsaI GGTCTCn'nnnn_
Cuts at: 0 58 523 3029 3363
Size: 58 465 2506 334
Fragments arranged by size:
2506 465 334 58
BsaAI yAC'GTr
Cuts at: 0 1488 3363
Size: 1488 1875
BsaHI Gr'CG_yC
Cuts at: 0 302 355 1865 2096 3363
Size: 302 53 1510 231 1267
Fragments arranged by size:
1510 1267 302 231 53
BsaWI w'CCGG_w
Cuts at: 0 2585 2891 3363
Size: 2585 306 472
Fragments arranged by size:
2585 472 306
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1054 1084 1943 1973 2305 2335 2431 2461
Size: 1054 30 859 30 332 30 96 30
Cuts at: 2461 2615 2645 3277 3307 3363
Size: 154 30 632 30 56
Fragments arranged by size:
1054 859 632 332 154 96 56 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 444 637 679 751 790 799 802 805
Size: 444 193 42 72 39 9 3 3
Cuts at: 805 808 811 820 823 826 829 856 859
Size: 3 3 9 3 3 3 27 3
Cuts at: 859 874 877 880 895 898 919 922 925
Size: 15 3 3 15 3 21 3 3
Cuts at: 925 928 2239 2251 2326 3019 3087 3142 3145
Size: 3 1311 12 75 693 68 55 3
Cuts at: 3145 3163 3363
Size: 18 200
Fragments arranged by size:
1311 693 444 200 193 75 72 68
55 42 39 27 21 18 15 15
12 9 9 3 3 3 3 3
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 1221 1919 1963 2050 2102 2128 3363
Size: 1221 698 44 87 52 26 1235
Fragments arranged by size:
1235 1221 698 87 52 44 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1455 1859 3097 3363
Size: 1455 404 1238 266
Fragments arranged by size:
1455 1238 404 266
BsiEI CG_ry'CG
Cuts at: 0 1620 3363
Size: 1620 1743
BsiHKAI G_wGCw'C
Cuts at: 0 30 206 587 983 3277 3363
Size: 30 176 381 396 2294 86
Fragments arranged by size:
2294 396 381 176 86 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 346 3363
Size: 346 3017
Bsp1286I G_dGCh'C
Cuts at: 0 30 119 206 406 587 983 991 1459
Size: 30 89 87 200 181 396 8 468
Cuts at: 1459 2533 2680 2702 3277 3363
Size: 1074 147 22 575 86
Fragments arranged by size:
1074 575 468 396 200 181 147 89
87 86 30 22 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 596 2842 3363
Size: 596 2246 521
Fragments arranged by size:
2246 596 521
BsrBI CCG'CTC
Cuts at: 0 1040 2316 3363
Size: 1040 1276 1047
Fragments arranged by size:
1276 1047 1040
BsrDI GCAATG_nn'
Cuts at: 0 1532 3363
Size: 1532 1831
BsrFI r'CCGG_y
Cuts at: 0 1679 2186 3363
Size: 1679 507 1177
Fragments arranged by size:
1679 1177 507
BssSI C'ACGA_G
Cuts at: 0 2606 3363
Size: 2606 757
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1184 2063 2923 3363
Size: 1184 879 860 440
Fragments arranged by size:
1184 879 860 440
BstEII G'GTnAC_C
Cuts at: 0 1746 3363
Size: 1746 1617
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1728 2872 3363
Size: 1728 1144 491
Fragments arranged by size:
1728 1144 491
BstYI r'GATC_y
Cuts at: 0 424 1290 1412 1424 1590 1622 3029 3092
Size: 424 866 122 12 166 32 1407 63
Cuts at: 3092 3363
Size: 271
Fragments arranged by size:
1407 866 424 271 166 122 63 32
12
Bsu36I CC'TnA_GG
Cuts at: 0 1627 2234 3363
Size: 1627 607 1129
Fragments arranged by size:
1627 1129 607
BtgI C'CryG_G
Cuts at: 0 3071 3101 3363
Size: 3071 30 262
Fragments arranged by size:
3071 262 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 361 474 1137 3363
Size: 361 113 663 2226
Fragments arranged by size:
2226 663 361 113
BtsI GCAGTG_nn'
Cuts at: 0 285 446 1889 3363
Size: 285 161 1443 1474
Fragments arranged by size:
1474 1443 285 161
DraIII CAC_nnn'GTG
Cuts at: 0 210 3363
Size: 210 3153
EaeI y'GGCC_r
Cuts at: 0 167 560 1350 1718 2424 2556 2756 2862
Size: 167 393 790 368 706 132 200 106
Cuts at: 2862 3363
Size: 501
Fragments arranged by size:
790 706 501 393 368 200 167 132
106
EarI CTCTTCn'nnn_
Cuts at: 0 169 521 536 646 767 797 821 827
Size: 169 352 15 110 121 30 24 6
Cuts at: 827 833 845 851 866 872 884 890 896
Size: 6 12 6 15 6 12 6 6
Cuts at: 896 1267 2294 3363
Size: 371 1027 1069
Fragments arranged by size:
1069 1027 371 352 169 121 110 30
24 15 15 12 12 6 6 6
6 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3211 3363
Size: 3211 152
EcoICRI GAG'CTC
Cuts at: 0 28 981 3363
Size: 28 953 2382
Fragments arranged by size:
2382 953 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 205 256 355 413 545 577 739
Size: 120 85 51 99 58 132 32 162
Cuts at: 739 1162 1180 1471 1750 2294 2377 2836 3136
Size: 423 18 291 279 544 83 459 300
Cuts at: 3136 3193 3346 3363
Size: 57 153 17
Fragments arranged by size:
544 459 423 300 291 279 162 153
132 120 99 85 83 58 57 51
32 18 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 1144 2221 2730 3190 3363
Size: 592 552 1077 509 460 173
Fragments arranged by size:
1077 592 552 509 460 173
EcoO109I rG'GnC_Cy
Cuts at: 0 45 55 571 636 699 972 987 1659
Size: 45 10 516 65 63 273 15 672
Cuts at: 1659 1956 2658 2780 2958 3195 3303 3318 3363
Size: 297 702 122 178 237 108 15 45
Fragments arranged by size:
702 672 516 297 273 237 178 122
108 65 63 45 45 15 15 10
EcoRV GAT'ATC
Cuts at: 0 1980 3363
Size: 1980 1383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 657 1767 3363
Size: 657 1110 1596
Fragments arranged by size:
1596 1110 657
FspI TGC'GCA
Cuts at: 0 1177 3363
Size: 1177 2186
FspAI rTGC'GCAy
Cuts at: 0 1177 3363
Size: 1177 2186
HaeII r_GCGC'y
Cuts at: 0 194 446 693 2109 2126 3363
Size: 194 252 247 1416 17 1237
Fragments arranged by size:
1416 1237 252 247 194 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1054 1086 1308 1340 1575 1607 3277 3309
Size: 1054 32 222 32 235 32 1670 32
Cuts at: 3309 3363
Size: 54
Fragments arranged by size:
1670 1054 235 222 54 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1082 3363
Size: 1082 2281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1079 1351 2276 2972 3074 3320 3363
Size: 452 627 272 925 696 102 246 43
Fragments arranged by size:
925 696 627 452 272 246 102 43
MscI TGG'CCA
Cuts at: 0 169 1352 1720 2558 2864 3363
Size: 169 1183 368 838 306 499
Fragments arranged by size:
1183 838 499 368 306 169
MslI CAynn'nnrTG
Cuts at: 0 292 2870 3286 3363
Size: 292 2578 416 77
Fragments arranged by size:
2578 416 292 77
MspA1I CmG'CkG
Cuts at: 0 73 86 391 1221 2063 2423 2714 3018
Size: 73 13 305 830 842 360 291 304
Cuts at: 3018 3081 3363
Size: 63 282
Fragments arranged by size:
842 830 360 305 304 291 282 73
63 13
NcoI C'CATG_G
Cuts at: 0 3071 3101 3363
Size: 3071 30 262
Fragments arranged by size:
3071 262 30
NdeI CA'TA_TG
Cuts at: 0 1668 3363
Size: 1668 1695
NsiI A_TGCA'T
Cuts at: 0 1439 3363
Size: 1439 1924
NspI r_CATG'y
Cuts at: 0 1797 2019 3291 3363
Size: 1797 222 1272 72
Fragments arranged by size:
1797 1272 222 72
PasI CC'CwG_GG
Cuts at: 0 1706 3363
Size: 1706 1657
PciI A'CATG_T
Cuts at: 0 3287 3363
Size: 3287 76
PflMI CCAn_nnn'nTGG
Cuts at: 0 574 717 3363
Size: 574 143 2646
Fragments arranged by size:
2646 574 143
PfoI T'CCnGG_A
Cuts at: 0 1072 1139 2803 3323 3363
Size: 1072 67 1664 520 40
Fragments arranged by size:
1664 1072 520 67 40
PmlI CAC'GTG
Cuts at: 0 1488 3363
Size: 1488 1875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2431 2463 3363
Size: 2431 32 900
Fragments arranged by size:
2431 900 32
PpuMI rG'GwC_Cy
Cuts at: 0 55 571 699 1659 1956 2658 2780 2958
Size: 55 516 128 960 297 702 122 178
Cuts at: 2958 3303 3318 3363
Size: 345 15 45
Fragments arranged by size:
960 702 516 345 297 178 128 122
55 45 15
PshAI GACnn'nnGTC
Cuts at: 0 704 3363
Size: 704 2659
PspOMI G'GGCC_C
Cuts at: 0 987 3363
Size: 987 2376
PspXI vC'TCGA_Gb
Cuts at: 0 1654 3363
Size: 1654 1709
PstI C_TGCA'G
Cuts at: 0 78 606 2068 3086 3363
Size: 78 528 1462 1018 277
Fragments arranged by size:
1462 1018 528 277 78
PvuI CG_AT'CG
Cuts at: 0 1620 3363
Size: 1620 1743
PvuII CAG'CTG
Cuts at: 0 73 86 391 1221 2063 3018 3081 3363
Size: 73 13 305 830 842 955 63 282
Fragments arranged by size:
955 842 830 305 282 73 63 13
SacI G_AGCT'C
Cuts at: 0 30 983 3363
Size: 30 953 2380
Fragments arranged by size:
2380 953 30
SanDI GG'GwC_CC
Cuts at: 0 2780 3363
Size: 2780 583
ScaI AGT'ACT
Cuts at: 0 133 1018 3363
Size: 133 885 2345
Fragments arranged by size:
2345 885 133
SexAI A'CCwGG_T
Cuts at: 0 206 3363
Size: 206 3157
SfcI C'TryA_G
Cuts at: 0 74 468 602 1281 2064 3082 3363
Size: 74 394 134 679 783 1018 281
Fragments arranged by size:
1018 783 679 394 281 134 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1717 3363
Size: 1717 1646
SmaI CCC'GGG
Cuts at: 0 1123 2846 3363
Size: 1123 1723 517
Fragments arranged by size:
1723 1123 517
SmlI C'TyrA_G
Cuts at: 0 1654 2010 2524 3363
Size: 1654 356 514 839
Fragments arranged by size:
1654 839 514 356
SphI G_CATG'C
Cuts at: 0 1797 2019 3363
Size: 1797 222 1344
Fragments arranged by size:
1797 1344 222
StuI AGG'CCT
Cuts at: 0 2164 3363
Size: 2164 1199
StyI C'CwwG_G
Cuts at: 0 41 458 1721 1742 2369 2642 3071 3101
Size: 41 417 1263 21 627 273 429 30
Cuts at: 3101 3363
Size: 262
Fragments arranged by size:
1263 627 429 417 273 262 41 30
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1014 1081 1514 1719 2652 2692 2844 2902
Size: 1014 67 433 205 933 40 152 58
Cuts at: 2902 3363
Size: 461
Fragments arranged by size:
1014 933 461 433 205 152 67 58
40
TatI w'GTAC_w
Cuts at: 0 131 1016 3290 3363
Size: 131 885 2274 73
Fragments arranged by size:
2274 885 131 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 196 228 1943 1975 2615 2647 3363
Size: 196 32 1715 32 640 32 716
Fragments arranged by size:
1715 716 640 196 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1713 1971 3057 3363
Size: 1713 258 1086 306
Fragments arranged by size:
1713 1086 306 258
XhoI C'TCGA_G
Cuts at: 0 1654 3363
Size: 1654 1709
XmaI C'CCGG_G
Cuts at: 0 1121 2844 3363
Size: 1121 1723 519
Fragments arranged by size:
1723 1121 519
ZraI GAC'GTC
Cuts at: 0 356 3363
Size: 356 3007
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI FspI FspAI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NcoI NdeI NsiI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII SacI SanDI ScaI SexAI
SfcI SfiI SmaI SmlI SphI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BclI BmtI BsaBI BsiWI
BsmI BspEI BspHI BsrGI BssHII BstBI BstZ17I ClaI
CspCI DraI DrdI EagI EcoRI FseI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI PacI PmeI PsiI PsrI RsrII SacII
SalI SapI SbfI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XmnI