Restriction map for
pF1KSDA0839
(Linear) (Six-base) MAPSORT of: KIAA0839.seq Check: 4909 from: 1 to: 4179
KIAA0839.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 80 903 4179
Size: 80 823 3276
Fragments arranged by size:
3276 823 80
AccI GT'mk_AC
Cuts at: 0 771 4179
Size: 771 3408
Acc65I G'GTAC_C
Cuts at: 0 1271 4179
Size: 1271 2908
AclI AA'CG_TT
Cuts at: 0 1607 1806 4179
Size: 1607 199 2373
Fragments arranged by size:
2373 1607 199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 467 2680 2835 2879 3304 3538 4070
Size: 200 267 2213 155 44 425 234 532
Cuts at: 4070 4179
Size: 109
Fragments arranged by size:
2213 532 425 267 234 200 155 109
44
AflII C'TTAA_G
Cuts at: 0 2038 4179
Size: 2038 2141
AflIII A'CryG_T
Cuts at: 0 3141 4179
Size: 3141 1038
AleI CACnn'nnGTG
Cuts at: 0 4051 4179
Size: 4051 128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2555 3063 4179
Size: 2555 508 1116
Fragments arranged by size:
2555 1116 508
AlwNI CAG_nnn'CTG
Cuts at: 0 1585 2703 3912 4179
Size: 1585 1118 1209 267
Fragments arranged by size:
1585 1209 1118 267
ApoI r'AATT_y
Cuts at: 0 1359 1418 1956 2575 3621 4179
Size: 1359 59 538 619 1046 558
Fragments arranged by size:
1359 1046 619 558 538 59
AseI AT'TA_AT
Cuts at: 0 1741 4179
Size: 1741 2438
AvaI C'yCGr_G
Cuts at: 0 50 275 4179
Size: 50 225 3904
Fragments arranged by size:
3904 225 50
BamHI G'GATC_C
Cuts at: 0 3615 4179
Size: 3615 564
BanI G'GyrC_C
Cuts at: 0 94 1271 3363 4179
Size: 94 1177 2092 816
Fragments arranged by size:
2092 1177 816 94
BanII G_rGCy'C
Cuts at: 0 3026 4179
Size: 3026 1153
BbeI G_GCGC'C
Cuts at: 0 98 4179
Size: 98 4081
BbsI GAAGACnn'nnnn_
Cuts at: 0 850 2371 3526 4179
Size: 850 1521 1155 653
Fragments arranged by size:
1521 1155 850 653
BbvCI CC'TCA_GC
Cuts at: 0 88 2297 4179
Size: 88 2209 1882
Fragments arranged by size:
2209 1882 88
BciVI GTATCCnnnnn_n'
Cuts at: 0 421 1505 3369 4179
Size: 421 1084 1864 810
Fragments arranged by size:
1864 1084 810 421
BclI T'GATC_A
Cuts at: 0 795 834 4179
Size: 795 39 3345
Fragments arranged by size:
3345 795 39
BfrBI ATG'CAT
Cuts at: 0 3565 4179
Size: 3565 614
BglII A'GATC_T
Cuts at: 0 1562 3323 4179
Size: 1562 1761 856
Fragments arranged by size:
1761 1562 856
BlpI GC'TnA_GC
Cuts at: 0 2756 4179
Size: 2756 1423
BmrI ACTGGGnnnn_n'
Cuts at: 0 107 138 452 544 4179
Size: 107 31 314 92 3635
Fragments arranged by size:
3635 314 107 92 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3489 3530 4179
Size: 3489 41 649
Fragments arranged by size:
3489 649 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 378 2029 2698 4179
Size: 25 353 1651 669 1481
Fragments arranged by size:
1651 1481 669 353 25
Bpu10I CC'TnA_GC
Cuts at: 0 88 2297 2401 4179
Size: 88 2209 104 1778
Fragments arranged by size:
2209 1778 104 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1894 2959 3040 4179
Size: 1894 1065 81 1139
Fragments arranged by size:
1894 1139 1065 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 2422 4179
Size: 2422 1757
BsaHI Gr'CG_yC
Cuts at: 0 95 447 1152 4179
Size: 95 352 705 3027
Fragments arranged by size:
3027 705 352 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1686 1716 3714 3744 4179
Size: 1686 30 1998 30 435
Fragments arranged by size:
1998 1686 435 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 447 4179
Size: 94 353 3732
Fragments arranged by size:
3732 353 94
BseYI C'CCAG_C
Cuts at: 0 3050 4179
Size: 3050 1129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2528 3686 4179
Size: 2528 1158 493
Fragments arranged by size:
2528 1158 493
BsiHKAI G_wGCw'C
Cuts at: 0 405 521 1438 2171 3026 3470 3487 3920
Size: 405 116 917 733 855 444 17 433
Cuts at: 3920 4179
Size: 259
Fragments arranged by size:
917 855 733 444 433 405 259 116
17
BsmI GAATG_Cn'
Cuts at: 0 2532 4179
Size: 2532 1647
Bsp1286I G_dGCh'C
Cuts at: 0 405 521 1438 2171 3026 3470 3487 3920
Size: 405 116 917 733 855 444 17 433
Cuts at: 3920 4179
Size: 259
Fragments arranged by size:
917 855 733 444 433 405 259 116
17
BspHI T'CATG_A
Cuts at: 0 3503 3882 4179
Size: 3503 379 297
Fragments arranged by size:
3503 379 297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 469 903 4179
Size: 80 389 434 3276
Fragments arranged by size:
3276 434 389 80
BsrBI CCG'CTC
Cuts at: 0 2509 4179
Size: 2509 1670
BsrDI GCAATG_nn'
Cuts at: 0 2810 3042 3155 3290 4028 4179
Size: 2810 232 113 135 738 151
Fragments arranged by size:
2810 738 232 151 135 113
BstAPI GCAn_nnn'nTGC
Cuts at: 0 712 2469 4179
Size: 712 1757 1710
Fragments arranged by size:
1757 1710 712
BstYI r'GATC_y
Cuts at: 0 83 1562 3078 3312 3323 3615 3705 4179
Size: 83 1479 1516 234 11 292 90 474
Fragments arranged by size:
1516 1479 474 292 234 90 83 11
BtgI C'CryG_G
Cuts at: 0 1155 2455 3398 4179
Size: 1155 1300 943 781
Fragments arranged by size:
1300 1155 943 781
BtsI GCAGTG_nn'
Cuts at: 0 1479 2746 4179
Size: 1479 1267 1433
Fragments arranged by size:
1479 1433 1267
ClaI AT'CG_AT
Cuts at: 0 2423 4179
Size: 2423 1756
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 843 878 3717 3752 4179
Size: 843 35 2839 35 427
Fragments arranged by size:
2839 843 427 35 35
DraI TTT'AAA
Cuts at: 0 1520 1859 2841 4179
Size: 1520 339 982 1338
Fragments arranged by size:
1520 1338 982 339
DraIII CAC_nnn'GTG
Cuts at: 0 1630 1668 4004 4179
Size: 1630 38 2336 175
Fragments arranged by size:
2336 1630 175 38
DrdI GACnn_nn'nnGTC
Cuts at: 0 3892 4179
Size: 3892 287
EaeI y'GGCC_r
Cuts at: 0 2417 3754 4179
Size: 2417 1337 425
Fragments arranged by size:
2417 1337 425
EarI CTCTTCn'nnn_
Cuts at: 0 611 686 1633 2186 3710 3857 4172 4179
Size: 611 75 947 553 1524 147 315 7
Fragments arranged by size:
1524 947 611 553 315 147 75 7
EcoICRI GAG'CTC
Cuts at: 0 3024 4179
Size: 3024 1155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 200 378 467 2029 2680 2698 2835
Size: 25 175 178 89 1562 651 18 137
Cuts at: 2835 2879 3304 3538 4070 4179
Size: 44 425 234 532 109
Fragments arranged by size:
1562 651 532 425 234 178 175 137
109 89 44 25 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 76 102 1454 4179
Size: 76 26 1352 2725
Fragments arranged by size:
2725 1352 76 26
EcoO109I rG'GnC_Cy
Cuts at: 0 36 108 546 1311 1447 2610 3894 4035
Size: 36 72 438 765 136 1163 1284 141
Cuts at: 4035 4179
Size: 144
Fragments arranged by size:
1284 1163 765 438 144 141 136 72
36
EcoRV GAT'ATC
Cuts at: 0 2552 4179
Size: 2552 1627
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2053 3913 4179
Size: 2053 1860 266
Fragments arranged by size:
2053 1860 266
FspI TGC'GCA
Cuts at: 0 2473 2522 4179
Size: 2473 49 1657
Fragments arranged by size:
2473 1657 49
FspAI rTGC'GCAy
Cuts at: 0 2473 4179
Size: 2473 1706
HaeII r_GCGC'y
Cuts at: 0 98 4179
Size: 98 4081
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1567 1599 2428 2460 4179
Size: 1567 32 829 32 1719
Fragments arranged by size:
1719 1567 829 32 32
HincII GTy'rAC
Cuts at: 0 2925 3849 4179
Size: 2925 924 330
Fragments arranged by size:
2925 924 330
HindIII A'AGCT_T
Cuts at: 0 1778 4179
Size: 1778 2401
HpaI GTT'AAC
Cuts at: 0 2925 4179
Size: 2925 1254
KasI G'GCGC_C
Cuts at: 0 94 4179
Size: 94 4085
KpnI G_GTAC'C
Cuts at: 0 1275 4179
Size: 1275 2904
MfeI C'AATT_G
Cuts at: 0 4058 4179
Size: 4058 121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1033 3207 4179
Size: 274 759 2174 972
Fragments arranged by size:
2174 972 759 274
MscI TGG'CCA
Cuts at: 0 2419 3756 4179
Size: 2419 1337 423
Fragments arranged by size:
2419 1337 423
MslI CAynn'nnrTG
Cuts at: 0 469 803 3283 4051 4179
Size: 469 334 2480 768 128
Fragments arranged by size:
2480 768 469 334 128
MspA1I CmG'CkG
Cuts at: 0 92 640 2301 2676 3670 3909 4179
Size: 92 548 1661 375 994 239 270
Fragments arranged by size:
1661 994 548 375 270 239 92
NarI GG'CG_CC
Cuts at: 0 95 4179
Size: 95 4084
NcoI C'CATG_G
Cuts at: 0 1155 2455 4179
Size: 1155 1300 1724
Fragments arranged by size:
1724 1300 1155
NdeI CA'TA_TG
Cuts at: 0 1168 4179
Size: 1168 3011
NsiI A_TGCA'T
Cuts at: 0 3567 4179
Size: 3567 612
NspI r_CATG'y
Cuts at: 0 1263 2527 3040 3145 4179
Size: 1263 1264 513 105 1034
Fragments arranged by size:
1264 1263 1034 513 105
PciI A'CATG_T
Cuts at: 0 3141 4179
Size: 3141 1038
PflMI CCAn_nnn'nTGG
Cuts at: 0 937 1043 1585 4179
Size: 937 106 542 2594
Fragments arranged by size:
2594 937 542 106
PfoI T'CCnGG_A
Cuts at: 0 32 4179
Size: 32 4147
PpuMI rG'GwC_Cy
Cuts at: 0 36 108 546 1311 1447 3894 4035 4179
Size: 36 72 438 765 136 2447 141 144
Fragments arranged by size:
2447 765 438 144 141 136 72 36
PsiI TTA'TAA
Cuts at: 0 758 4179
Size: 758 3421
PstI C_TGCA'G
Cuts at: 0 717 1486 2753 4179
Size: 717 769 1267 1426
Fragments arranged by size:
1426 1267 769 717
PvuII CAG'CTG
Cuts at: 0 640 2301 2676 3670 3909 4179
Size: 640 1661 375 994 239 270
Fragments arranged by size:
1661 994 640 375 270 239
SacI G_AGCT'C
Cuts at: 0 3026 4179
Size: 3026 1153
SanDI GG'GwC_CC
Cuts at: 0 108 4179
Size: 108 4071
ScaI AGT'ACT
Cuts at: 0 554 2653 4179
Size: 554 2099 1526
Fragments arranged by size:
2099 1526 554
SfcI C'TryA_G
Cuts at: 0 713 917 1304 1482 1897 2749 3874 4179
Size: 713 204 387 178 415 852 1125 305
Fragments arranged by size:
1125 852 713 415 387 305 204 178
SfoI GGC'GCC
Cuts at: 0 96 4179
Size: 96 4083
SmaI CCC'GGG
Cuts at: 0 52 4179
Size: 52 4127
SmlI C'TyrA_G
Cuts at: 0 275 1873 2038 2938 3019 4179
Size: 275 1598 165 900 81 1160
Fragments arranged by size:
1598 1160 900 275 165 81
SpeI A'CTAG_T
Cuts at: 0 4077 4179
Size: 4077 102
SphI G_CATG'C
Cuts at: 0 3040 4179
Size: 3040 1139
SspI AAT'ATT
Cuts at: 0 2146 3131 4179
Size: 2146 985 1048
Fragments arranged by size:
2146 1048 985
StyI C'CwwG_G
Cuts at: 0 1155 1652 2455 2767 4179
Size: 1155 497 803 312 1412
Fragments arranged by size:
1412 1155 803 497 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2584 3741 3970 4011 4179
Size: 2584 1157 229 41 168
Fragments arranged by size:
2584 1157 229 168 41
TatI w'GTAC_w
Cuts at: 0 552 2651 4006 4179
Size: 552 2099 1355 173
Fragments arranged by size:
2099 1355 552 173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 303 335 432 464 3714 3746 4179
Size: 303 32 97 32 3250 32 433
Fragments arranged by size:
3250 433 303 97 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 822 4179
Size: 822 3357
XbaI T'CTAG_A
Cuts at: 0 1147 4179
Size: 1147 3032
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 1713 3423 4179
Size: 261 1452 1710 756
Fragments arranged by size:
1710 1452 756 261
XhoI C'TCGA_G
Cuts at: 0 275 4179
Size: 275 3904
XmaI C'CCGG_G
Cuts at: 0 50 4179
Size: 50 4129
XmnI GAAnn'nnTTC
Cuts at: 0 1742 3178 4179
Size: 1742 1436 1001
Fragments arranged by size:
1742 1436 1001
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AlwNI ApoI AseI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BstAPI BstYI BtgI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MmeI MscI MslI
MspA1I NarI NcoI NdeI NsiI NspI PciI PflMI
PfoI PpuMI PsiI PstI PvuII SacI SanDI ScaI
SfcI SfoI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AloI ApaI ApaLI AscI
AsiSI AvrII BaeI BcgI BglI Bme1580I BmgBI BmtI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI EagI EciI EcoRI FseI MluI NaeI NgoMIV
NheI NotI NruI PacI PasI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SrfI
StuI SwaI ZraI