Restriction map for
pF1KA0847
(Linear) (Six-base) MAPSORT of: KIAA0847.seq Check: 2632 from: 1 to: 3732
KIAA0847.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AatII G_ACGT'C
Cuts at: 0 530 3732
Size: 530 3202
AccI GT'mk_AC
Cuts at: 0 366 3732
Size: 366 3366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1226 1666 1769 1871 2024 2068 2179
Size: 166 1060 440 103 102 153 44 111
Cuts at: 2179 2351 2537 3128 3732
Size: 172 186 591 604
Fragments arranged by size:
1060 604 591 440 186 172 166 153
111 103 102 44
AflIII A'CryG_T
Cuts at: 0 463 641 2219 2583 3732
Size: 463 178 1578 364 1149
Fragments arranged by size:
1578 1149 463 364 178
AhdI GACnn_n'nnGTC
Cuts at: 0 627 3732
Size: 627 3105
AlwNI CAG_nnn'CTG
Cuts at: 0 1387 2887 3732
Size: 1387 1500 845
Fragments arranged by size:
1500 1387 845
ApoI r'AATT_y
Cuts at: 0 101 760 1187 2305 2558 3155 3338 3732
Size: 101 659 427 1118 253 597 183 394
Fragments arranged by size:
1118 659 597 427 394 253 183 101
AvaI C'yCGr_G
Cuts at: 0 326 539 3732
Size: 326 213 3193
Fragments arranged by size:
3193 326 213
AvrII C'CTAG_G
Cuts at: 0 3291 3732
Size: 3291 441
BamHI G'GATC_C
Cuts at: 0 3652 3732
Size: 3652 80
BanI G'GyrC_C
Cuts at: 0 2499 2776 3732
Size: 2499 277 956
Fragments arranged by size:
2499 956 277
BanII G_rGCy'C
Cuts at: 0 1624 2099 3732
Size: 1624 475 1633
Fragments arranged by size:
1633 1624 475
BbsI GAAGACnn'nnnn_
Cuts at: 0 618 901 1231 2666 3732
Size: 618 283 330 1435 1066
Fragments arranged by size:
1435 1066 618 330 283
BclI T'GATC_A
Cuts at: 0 1818 2280 3732
Size: 1818 462 1452
Fragments arranged by size:
1818 1452 462
BfrBI ATG'CAT
Cuts at: 0 577 3732
Size: 577 3155
BglII A'GATC_T
Cuts at: 0 306 2079 2402 3732
Size: 306 1773 323 1330
Fragments arranged by size:
1773 1330 323 306
BlpI GC'TnA_GC
Cuts at: 0 1054 3732
Size: 1054 2678
Bme1580I G_kGCm'C
Cuts at: 0 2781 3732
Size: 2781 951
BmgBI CAC'GTC
Cuts at: 0 3488 3732
Size: 3488 244
BmrI ACTGGGnnnn_n'
Cuts at: 0 897 3132 3446 3732
Size: 897 2235 314 286
Fragments arranged by size:
2235 897 314 286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2424 2874 3392 3732
Size: 2424 450 518 340
Fragments arranged by size:
2424 518 450 340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 740 1025 1390 1432 1777 1786 1900
Size: 696 44 285 365 42 345 9 114
Cuts at: 1900 1943 2864 3278 3582 3732
Size: 43 921 414 304 150
Fragments arranged by size:
921 696 414 365 345 304 285 150
114 44 43 42 9
Bpu10I CC'TnA_GC
Cuts at: 0 1571 1618 3732
Size: 1571 47 2114
Fragments arranged by size:
2114 1571 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1420 1763 2237 2970 3732
Size: 1420 343 474 733 762
Fragments arranged by size:
1420 762 733 474 343
BsaI GGTCTCn'nnnn_
Cuts at: 0 1396 2675 3732
Size: 1396 1279 1057
Fragments arranged by size:
1396 1279 1057
BsaHI Gr'CG_yC
Cuts at: 0 527 1951 3732
Size: 527 1424 1781
Fragments arranged by size:
1781 1424 527
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2411 2441 3307 3337 3438 3468 3732
Size: 2411 30 866 30 101 30 264
Fragments arranged by size:
2411 866 264 101 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 26 1225 1756 2397 2893 3025 3061 3389
Size: 26 1199 531 641 496 132 36 328
Cuts at: 3389 3452 3549 3585 3732
Size: 63 97 36 147
Fragments arranged by size:
1199 641 531 496 328 147 132 97
63 36 36 26
BseYI C'CCAG_C
Cuts at: 0 360 3574 3631 3732
Size: 360 3214 57 101
Fragments arranged by size:
3214 360 101 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1457 3732
Size: 1457 2275
BsiHKAI G_wGCw'C
Cuts at: 0 1624 3059 3373 3732
Size: 1624 1435 314 359
Fragments arranged by size:
1624 1435 359 314
BsmI GAATG_Cn'
Cuts at: 0 392 3732
Size: 392 3340
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3215 3732
Size: 3215 517
Bsp1286I G_dGCh'C
Cuts at: 0 1624 2099 2781 3059 3373 3732
Size: 1624 475 682 278 314 359
Fragments arranged by size:
1624 682 475 359 314 278
BspHI T'CATG_A
Cuts at: 0 2214 2651 3732
Size: 2214 437 1081
Fragments arranged by size:
2214 1081 437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 541 1414 3732
Size: 541 873 2318
Fragments arranged by size:
2318 873 541
BsrBI CCG'CTC
Cuts at: 0 1280 3732
Size: 1280 2452
BsrDI GCAATG_nn'
Cuts at: 0 1602 3621 3732
Size: 1602 2019 111
Fragments arranged by size:
2019 1602 111
BssSI C'ACGA_G
Cuts at: 0 3249 3732
Size: 3249 483
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1861 3732
Size: 1861 1871
BstEII G'GTnAC_C
Cuts at: 0 1262 3732
Size: 1262 2470
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1234 3173 3732
Size: 1234 1939 559
Fragments arranged by size:
1939 1234 559
BstYI r'GATC_y
Cuts at: 0 306 1108 2079 2402 3352 3394 3586 3652
Size: 306 802 971 323 950 42 192 66
Cuts at: 3652 3732
Size: 80
Fragments arranged by size:
971 950 802 323 306 192 80 66
42
Bsu36I CC'TnA_GG
Cuts at: 0 1839 3732
Size: 1839 1893
BtgI C'CryG_G
Cuts at: 0 1221 2090 3732
Size: 1221 869 1642
Fragments arranged by size:
1642 1221 869
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1073 3732
Size: 1073 2659
ClaI AT'CG_AT
Cuts at: 0 261 3732
Size: 261 3471
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 501 536 3732
Size: 501 35 3196
Fragments arranged by size:
3196 501 35
DraI TTT'AAA
Cuts at: 0 1826 3732
Size: 1826 1906
DrdI GACnn_nn'nnGTC
Cuts at: 0 1124 3732
Size: 1124 2608
EaeI y'GGCC_r
Cuts at: 0 1224 3226 3732
Size: 1224 2002 506
Fragments arranged by size:
2002 1224 506
EarI CTCTTCn'nnn_
Cuts at: 0 792 1145 1202 2327 3697 3732
Size: 792 353 57 1125 1370 35
Fragments arranged by size:
1370 1125 792 353 57 35
EcoICRI GAG'CTC
Cuts at: 0 1622 3732
Size: 1622 2110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 696 740 1025 1226 1390 1432 1666
Size: 166 530 44 285 201 164 42 234
Cuts at: 1666 1769 1777 1786 1871 1900 1943 2024 2068
Size: 103 8 9 85 29 43 81 44
Cuts at: 2068 2179 2351 2537 2864 3128 3278 3582 3732
Size: 111 172 186 327 264 150 304 150
Fragments arranged by size:
530 327 304 285 264 234 201 186
172 166 164 150 150 111 103 85
81 44 44 43 42 29 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2168 3597 3600 3732
Size: 2168 1429 3 132
Fragments arranged by size:
2168 1429 132 3
EcoO109I rG'GnC_Cy
Cuts at: 0 1677 1801 1870 1984 3605 3732
Size: 1677 124 69 114 1621 127
Fragments arranged by size:
1677 1621 127 124 114 69
EcoRI G'AATT_C
Cuts at: 0 760 3732
Size: 760 2972
EcoRV GAT'ATC
Cuts at: 0 27 2633 3732
Size: 27 2606 1099
Fragments arranged by size:
2606 1099 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1549 2005 3732
Size: 1549 456 1727
Fragments arranged by size:
1727 1549 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 243 275 613 645 769 801 2394 2426
Size: 243 32 338 32 124 32 1593 32
Cuts at: 2426 2708 2740 3272 3304 3307 3339 3732
Size: 282 32 532 32 3 32 393
Fragments arranged by size:
1593 532 393 338 282 243 124 32
32 32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 0 1614 2036 3180 3732
Size: 1614 422 1144 552
Fragments arranged by size:
1614 1144 552 422
HindIII A'AGCT_T
Cuts at: 0 1180 2046 2533 2938 3732
Size: 1180 866 487 405 794
Fragments arranged by size:
1180 866 794 487 405
HpaI GTT'AAC
Cuts at: 0 1614 3732
Size: 1614 2118
MfeI C'AATT_G
Cuts at: 0 974 3732
Size: 974 2758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 274 2526 3332 3732
Size: 18 256 2252 806 400
Fragments arranged by size:
2252 806 400 256 18
MscI TGG'CCA
Cuts at: 0 1226 3732
Size: 1226 2506
MslI CAynn'nnrTG
Cuts at: 0 646 1520 3732
Size: 646 874 2212
Fragments arranged by size:
2212 874 646
MspA1I CmG'CkG
Cuts at: 0 669 3106 3732
Size: 669 2437 626
Fragments arranged by size:
2437 669 626
NcoI C'CATG_G
Cuts at: 0 1221 2090 3732
Size: 1221 869 1642
Fragments arranged by size:
1642 1221 869
NsiI A_TGCA'T
Cuts at: 0 579 3732
Size: 579 3153
NspI r_CATG'y
Cuts at: 0 123 467 483 645 2223 2587 3732
Size: 123 344 16 162 1578 364 1145
Fragments arranged by size:
1578 1145 364 344 162 123 16
PasI CC'CwG_GG
Cuts at: 0 2320 3732
Size: 2320 1412
PciI A'CATG_T
Cuts at: 0 463 641 2219 2583 3732
Size: 463 178 1578 364 1149
Fragments arranged by size:
1578 1149 463 364 178
PflMI CCAn_nnn'nTGG
Cuts at: 0 1258 1652 3732
Size: 1258 394 2080
Fragments arranged by size:
2080 1258 394
PfoI T'CCnGG_A
Cuts at: 0 1763 3390 3598 3732
Size: 1763 1627 208 134
Fragments arranged by size:
1763 1627 208 134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 551 583 3732
Size: 551 32 3149
Fragments arranged by size:
3149 551 32
PpuMI rG'GwC_Cy
Cuts at: 0 1677 1801 1870 1984 3605 3732
Size: 1677 124 69 114 1621 127
Fragments arranged by size:
1677 1621 127 124 114 69
PspXI vC'TCGA_Gb
Cuts at: 0 539 3732
Size: 539 3193
PstI C_TGCA'G
Cuts at: 0 1866 3732
Size: 1866 1866
PvuII CAG'CTG
Cuts at: 0 669 3106 3732
Size: 669 2437 626
Fragments arranged by size:
2437 669 626
SacI G_AGCT'C
Cuts at: 0 1624 3732
Size: 1624 2108
ScaI AGT'ACT
Cuts at: 0 1754 3732
Size: 1754 1978
SfcI C'TryA_G
Cuts at: 0 1706 1862 3732
Size: 1706 156 1870
Fragments arranged by size:
1870 1706 156
SmlI C'TyrA_G
Cuts at: 0 539 1399 1778 2252 2985 3732
Size: 539 860 379 474 733 747
Fragments arranged by size:
860 747 733 539 474 379
StuI AGG'CCT
Cuts at: 0 3255 3732
Size: 3255 477
StyI C'CwwG_G
Cuts at: 0 113 1221 1257 1804 2090 2119 2973 3160
Size: 113 1108 36 547 286 29 854 187
Cuts at: 3160 3291 3696 3732
Size: 131 405 36
Fragments arranged by size:
1108 854 547 405 286 187 131 113
36 36 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1644 3141 3261 3435 3732
Size: 1644 1497 120 174 297
Fragments arranged by size:
1644 1497 297 174 120
TatI w'GTAC_w
Cuts at: 0 460 1752 3732
Size: 460 1292 1980
Fragments arranged by size:
1980 1292 460
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 32 64 3438 3470 3732
Size: 32 32 3374 32 262
Fragments arranged by size:
3374 262 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2932 3732
Size: 2932 800
XbaI T'CTAG_A
Cuts at: 0 774 3142 3732
Size: 774 2368 590
Fragments arranged by size:
2368 774 590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 357 1962 3732
Size: 357 1605 1770
Fragments arranged by size:
1770 1605 357
XhoI C'TCGA_G
Cuts at: 0 539 3732
Size: 539 3193
XmnI GAAnn'nnTTC
Cuts at: 0 392 1451 1532 1731 1754 2747 3732
Size: 392 1059 81 199 23 993 985
Fragments arranged by size:
1059 993 985 392 199 81 23
ZraI GAC'GTC
Cuts at: 0 528 3732
Size: 528 3204
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AlwNI ApoI AvaI
AvrII BamHI BanI BanII BbsI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI ClaI CspCI
DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII HpaI MfeI
MmeI MscI MslI MspA1I NcoI NsiI NspI PasI
PciI PflMI PfoI PpiI PpuMI PspXI PstI PvuII
SacI ScaI SfcI SmlI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI BaeI BbeI
BbvCI BcgI BciVI BglI BmtI BsaAI BsaBI BsaWI
BsiEI BsiWI BspEI BsrFI BsrGI BssHII BstBI BstZ17I
BtsI DraIII EagI EciI FseI FspI FspAI HaeII
KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PmeI PmlI PshAI PsiI PspOMI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XmaI