Restriction map for
pF1KSDA0849
(Linear) (Six-base) MAPSORT of: KIAA0849.seq Check: 9423 from: 1 to: 2859
KIAA0849.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 1439 1527 1594 1618 2042 2859
Size: 426 1013 88 67 24 424 817
Fragments arranged by size:
1013 817 426 424 88 67 24
AfeI AGC'GCT
Cuts at: 0 1660 2859
Size: 1660 1199
AflII C'TTAA_G
Cuts at: 0 1689 2859
Size: 1689 1170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 251 2838 2859
Size: 251 2587 21
Fragments arranged by size:
2587 251 21
AlwNI CAG_nnn'CTG
Cuts at: 0 655 1027 2859
Size: 655 372 1832
Fragments arranged by size:
1832 655 372
ApaLI G'TGCA_C
Cuts at: 0 2792 2859
Size: 2792 67
ApoI r'AATT_y
Cuts at: 0 218 421 2041 2138 2221 2294 2859
Size: 218 203 1620 97 83 73 565
Fragments arranged by size:
1620 565 218 203 97 83 73
BanI G'GyrC_C
Cuts at: 0 2232 2859
Size: 2232 627
BanII G_rGCy'C
Cuts at: 0 1411 2859
Size: 1411 1448
BbsI GAAGACnn'nnnn_
Cuts at: 0 1064 1142 2342 2759 2805 2859
Size: 1064 78 1200 417 46 54
Fragments arranged by size:
1200 1064 417 78 54 46
BfrBI ATG'CAT
Cuts at: 0 2815 2859
Size: 2815 44
BglII A'GATC_T
Cuts at: 0 406 1042 2859
Size: 406 636 1817
Fragments arranged by size:
1817 636 406
Bme1580I G_kGCm'C
Cuts at: 0 1572 2796 2859
Size: 1572 1224 63
Fragments arranged by size:
1572 1224 63
BmgBI CAC'GTC
Cuts at: 0 378 2859
Size: 378 2481
BmrI ACTGGGnnnn_n'
Cuts at: 0 54 834 1311 2494 2538 2859
Size: 54 780 477 1183 44 321
Fragments arranged by size:
1183 780 477 321 54 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 684 1180 2556 2757 2859
Size: 449 235 496 1376 201 102
Fragments arranged by size:
1376 496 449 235 201 102
Bpu10I CC'TnA_GC
Cuts at: 0 780 2859
Size: 780 2079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2441 2687 2859
Size: 2441 246 172
Fragments arranged by size:
2441 246 172
BsaWI w'CCGG_w
Cuts at: 0 475 2406 2859
Size: 475 1931 453
Fragments arranged by size:
1931 475 453
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 95 125 1178 1208 2859
Size: 95 30 1053 30 1651
Fragments arranged by size:
1651 1053 95 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 1193 2859
Size: 334 859 1666
Fragments arranged by size:
1666 859 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 1328 1541 2859
Size: 868 460 213 1318
Fragments arranged by size:
1318 868 460 213
BsiEI CG_ry'CG
Cuts at: 0 2670 2859
Size: 2670 189
BsiHKAI G_wGCw'C
Cuts at: 0 1493 2796 2859
Size: 1493 1303 63
Fragments arranged by size:
1493 1303 63
BsmI GAATG_Cn'
Cuts at: 0 85 168 2398 2760 2859
Size: 85 83 2230 362 99
Fragments arranged by size:
2230 362 99 85 83
Bsp1286I G_dGCh'C
Cuts at: 0 1411 1493 1572 2796 2859
Size: 1411 82 79 1224 63
Fragments arranged by size:
1411 1224 82 79 63
BspEI T'CCGG_A
Cuts at: 0 475 2406 2859
Size: 475 1931 453
Fragments arranged by size:
1931 475 453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 640 660 2095 2455 2765 2859
Size: 640 20 1435 360 310 94
Fragments arranged by size:
1435 640 360 310 94 20
BsrBI CCG'CTC
Cuts at: 0 54 2859
Size: 54 2805
BsrDI GCAATG_nn'
Cuts at: 0 1630 2381 2859
Size: 1630 751 478
Fragments arranged by size:
1630 751 478
BsrFI r'CCGG_y
Cuts at: 0 1638 2859
Size: 1638 1221
BsrGI T'GTAC_A
Cuts at: 0 2852 2859
Size: 2852 7
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2555 2859
Size: 2555 304
BstYI r'GATC_y
Cuts at: 0 406 1012 1042 2031 2859
Size: 406 606 30 989 828
Fragments arranged by size:
989 828 606 406 30
Bsu36I CC'TnA_GG
Cuts at: 0 2036 2859
Size: 2036 823
BtsI GCAGTG_nn'
Cuts at: 0 626 1340 2859
Size: 626 714 1519
Fragments arranged by size:
1519 714 626
DraI TTT'AAA
Cuts at: 0 339 2280 2289 2859
Size: 339 1941 9 570
Fragments arranged by size:
1941 570 339 9
EaeI y'GGCC_r
Cuts at: 0 1290 1370 2663 2859
Size: 1290 80 1293 196
Fragments arranged by size:
1293 1290 196 80
EarI CTCTTCn'nnn_
Cuts at: 0 44 1325 1594 2470 2653 2859
Size: 44 1281 269 876 183 206
Fragments arranged by size:
1281 876 269 206 183 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 449 684 1180 1439 1527 1594 1618
Size: 426 23 235 496 259 88 67 24
Cuts at: 1618 2042 2556 2757 2859
Size: 424 514 201 102
Fragments arranged by size:
514 496 426 424 259 235 201 102
88 67 24 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1576 2859
Size: 1576 1283
EcoO109I rG'GnC_Cy
Cuts at: 0 448 652 2859
Size: 448 204 2207
Fragments arranged by size:
2207 448 204
EcoRI G'AATT_C
Cuts at: 0 2041 2859
Size: 2041 818
EcoRV GAT'ATC
Cuts at: 0 903 1163 2859
Size: 903 260 1696
Fragments arranged by size:
1696 903 260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 197 2482 2859
Size: 197 2285 377
Fragments arranged by size:
2285 377 197
HaeII r_GCGC'y
Cuts at: 0 1585 1662 2859
Size: 1585 77 1197
Fragments arranged by size:
1585 1197 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1990 2022 2221 2253 2469 2501 2859
Size: 1990 32 199 32 216 32 358
Fragments arranged by size:
1990 358 216 199 32 32 32
HincII GTy'rAC
Cuts at: 0 1080 2859
Size: 1080 1779
HindIII A'AGCT_T
Cuts at: 0 777 2859
Size: 777 2082
MfeI C'AATT_G
Cuts at: 0 312 2859
Size: 312 2547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 1438 2859
Size: 699 739 1421
Fragments arranged by size:
1421 739 699
MscI TGG'CCA
Cuts at: 0 1292 1372 2859
Size: 1292 80 1487
Fragments arranged by size:
1487 1292 80
MslI CAynn'nnrTG
Cuts at: 0 896 1264 2238 2820 2859
Size: 896 368 974 582 39
Fragments arranged by size:
974 896 582 368 39
MspA1I CmG'CkG
Cuts at: 0 402 2419 2859
Size: 402 2017 440
Fragments arranged by size:
2017 440 402
NsiI A_TGCA'T
Cuts at: 0 2817 2859
Size: 2817 42
NspI r_CATG'y
Cuts at: 0 2829 2859
Size: 2829 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 2565 2859
Size: 2565 294
PfoI T'CCnGG_A
Cuts at: 0 426 2773 2859
Size: 426 2347 86
Fragments arranged by size:
2347 426 86
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 2859
Size: 310 32 2517
Fragments arranged by size:
2517 310 32
PpuMI rG'GwC_Cy
Cuts at: 0 448 652 2859
Size: 448 204 2207
Fragments arranged by size:
2207 448 204
PstI C_TGCA'G
Cuts at: 0 1609 2859
Size: 1609 1250
PvuI CG_AT'CG
Cuts at: 0 2670 2859
Size: 2670 189
PvuII CAG'CTG
Cuts at: 0 402 2419 2859
Size: 402 2017 440
Fragments arranged by size:
2017 440 402
SapI GCTCTTCn'nnn_
Cuts at: 0 44 1325 1594 2653 2859
Size: 44 1281 269 1059 206
Fragments arranged by size:
1281 1059 269 206 44
ScaI AGT'ACT
Cuts at: 0 2734 2859
Size: 2734 125
SfcI C'TryA_G
Cuts at: 0 1007 1166 1605 2859
Size: 1007 159 439 1254
Fragments arranged by size:
1254 1007 439 159
SmlI C'TyrA_G
Cuts at: 0 1689 2456 2702 2859
Size: 1689 767 246 157
Fragments arranged by size:
1689 767 246 157
SspI AAT'ATT
Cuts at: 0 482 1885 2052 2859
Size: 482 1403 167 807
Fragments arranged by size:
1403 807 482 167
StuI AGG'CCT
Cuts at: 0 365 936 1611 2859
Size: 365 571 675 1248
Fragments arranged by size:
1248 675 571 365
StyI C'CwwG_G
Cuts at: 0 543 1367 2765 2784 2859
Size: 543 824 1398 19 75
Fragments arranged by size:
1398 824 543 75 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1453 2859
Size: 1453 1406
TatI w'GTAC_w
Cuts at: 0 396 877 2732 2852 2859
Size: 396 481 1855 120 7
Fragments arranged by size:
1855 481 396 120 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 95 127 1178 1210 1501 1533 2859
Size: 95 32 1051 32 291 32 1326
Fragments arranged by size:
1326 1051 291 95 32 32 32
XbaI T'CTAG_A
Cuts at: 0 222 1398 2859
Size: 222 1176 1461
Fragments arranged by size:
1461 1176 222
XmnI GAAnn'nnTTC
Cuts at: 0 422 1234 2689 2859
Size: 422 812 1455 170
Fragments arranged by size:
1455 812 422 170
Enzymes that do cut:
AcuI AfeI AflII AlfI AlwNI ApaLI ApoI BanI
BanII BbsI BfrBI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstYI Bsu36I BtsI DraI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NsiI NspI
PflMI PfoI PpiI PpuMI PstI PvuI PvuII SapI
ScaI SfcI SmlI SspI StuI StyI TaqII TatI
TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglI
BlpI BmtI BplI BsaI BsaAI BsaBI BsaHI BseYI
BsiWI BsmBI BspHI BssHII BssSI BstBI BstEII BstXI
BstZ17I BtgI BtgZI ClaI CspCI DraIII DrdI EagI
EciI EcoICRI FseI FspI FspAI HpaI KasI KpnI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI Tth111I XcmI XhoI XmaI
ZraI