Restriction map for
pF1KA0850
(Linear) (Six-base) MAPSORT of: KIAA0850.seq Check: 6711 from: 1 to: 1926
KIAA0850.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1108 1926
Size: 1108 818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 355 515 863 1926
Size: 91 264 160 348 1063
Fragments arranged by size:
1063 348 264 160 91
AflII C'TTAA_G
Cuts at: 0 511 1926
Size: 511 1415
AhdI GACnn_n'nnGTC
Cuts at: 0 109 1926
Size: 109 1817
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1649 1681 1926
Size: 1649 32 245
Fragments arranged by size:
1649 245 32
AlwNI CAG_nnn'CTG
Cuts at: 0 244 1926
Size: 244 1682
ApoI r'AATT_y
Cuts at: 0 36 206 220 414 488 1852 1926
Size: 36 170 14 194 74 1364 74
Fragments arranged by size:
1364 194 170 74 74 36 14
BanII G_rGCy'C
Cuts at: 0 976 1218 1903 1926
Size: 976 242 685 23
Fragments arranged by size:
976 685 242 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 41 1135 1926
Size: 41 1094 791
Fragments arranged by size:
1094 791 41
BbvCI CC'TCA_GC
Cuts at: 0 722 1926
Size: 722 1204
BclI T'GATC_A
Cuts at: 0 681 1926
Size: 681 1245
Bme1580I G_kGCm'C
Cuts at: 0 1488 1926
Size: 1488 438
BmrI ACTGGGnnnn_n'
Cuts at: 0 609 1926
Size: 609 1317
BmtI G_CTAG'C
Cuts at: 0 151 1926
Size: 151 1775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1676 1926
Size: 1676 250
Bpu10I CC'TnA_GC
Cuts at: 0 722 854 1926
Size: 722 132 1072
Fragments arranged by size:
1072 722 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1903 1926
Size: 1903 23
BsaAI yAC'GTr
Cuts at: 0 778 1926
Size: 778 1148
BsaBI GATnn'nnATC
Cuts at: 0 194 233 1926
Size: 194 39 1693
Fragments arranged by size:
1693 194 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 466 496 972 1002 1643 1649 1673 1679
Size: 466 30 476 30 641 6 24 6
Cuts at: 1679 1926
Size: 247
Fragments arranged by size:
641 476 466 247 30 30 24 6
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 517 1664 1926
Size: 514 3 1147 262
Fragments arranged by size:
1147 514 262 3
BseYI C'CCAG_C
Cuts at: 0 565 1795 1926
Size: 565 1230 131
Fragments arranged by size:
1230 565 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 781 1577 1926
Size: 625 156 796 349
Fragments arranged by size:
796 625 349 156
BsiHKAI G_wGCw'C
Cuts at: 0 976 1380 1926
Size: 976 404 546
Fragments arranged by size:
976 546 404
BsmI GAATG_Cn'
Cuts at: 0 1162 1926
Size: 1162 764
Bsp1286I G_dGCh'C
Cuts at: 0 976 1218 1380 1488 1903 1926
Size: 976 242 162 108 415 23
Fragments arranged by size:
976 415 242 162 108 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 345 1108 1926
Size: 345 763 818
Fragments arranged by size:
818 763 345
BsrDI GCAATG_nn'
Cuts at: 0 576 1797 1854 1926
Size: 576 1221 57 72
Fragments arranged by size:
1221 576 72 57
BsrGI T'GTAC_A
Cuts at: 0 1541 1926
Size: 1541 385
BstBI TT'CG_AA
Cuts at: 0 1146 1926
Size: 1146 780
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1638 1926
Size: 1638 288
Bsu36I CC'TnA_GG
Cuts at: 0 73 1926
Size: 73 1853
BtsI GCAGTG_nn'
Cuts at: 0 149 272 741 1835 1926
Size: 149 123 469 1094 91
Fragments arranged by size:
1094 469 149 123 91
DraIII CAC_nnn'GTG
Cuts at: 0 778 1926
Size: 778 1148
EaeI y'GGCC_r
Cuts at: 0 84 117 786 1272 1926
Size: 84 33 669 486 654
Fragments arranged by size:
669 654 486 84 33
EarI CTCTTCn'nnn_
Cuts at: 0 491 635 647 831 1926
Size: 491 144 12 184 1095
Fragments arranged by size:
1095 491 184 144 12
EcoICRI GAG'CTC
Cuts at: 0 974 1926
Size: 974 952
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 355 515 863 1676 1926
Size: 91 264 160 348 813 250
Fragments arranged by size:
813 348 264 250 160 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 95 127 1168 1200 1926
Size: 95 32 1041 32 726
Fragments arranged by size:
1041 726 95 32 32
HindIII A'AGCT_T
Cuts at: 0 514 1011 1926
Size: 514 497 915
Fragments arranged by size:
915 514 497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 1765 1926
Size: 512 1253 161
Fragments arranged by size:
1253 512 161
MscI TGG'CCA
Cuts at: 0 86 119 788 1274 1926
Size: 86 33 669 486 652
Fragments arranged by size:
669 652 486 86 33
MslI CAynn'nnrTG
Cuts at: 0 1280 1636 1926
Size: 1280 356 290
Fragments arranged by size:
1280 356 290
MspA1I CmG'CkG
Cuts at: 0 398 679 1926
Size: 398 281 1247
Fragments arranged by size:
1247 398 281
NdeI CA'TA_TG
Cuts at: 0 1419 1926
Size: 1419 507
NheI G'CTAG_C
Cuts at: 0 147 1926
Size: 147 1779
PmlI CAC'GTG
Cuts at: 0 778 1926
Size: 778 1148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1649 1681 1926
Size: 1649 32 245
Fragments arranged by size:
1649 245 32
PshAI GACnn'nnGTC
Cuts at: 0 1509 1926
Size: 1509 417
PstI C_TGCA'G
Cuts at: 0 160 324 1119 1518 1926
Size: 160 164 795 399 408
Fragments arranged by size:
795 408 399 164 160
PvuII CAG'CTG
Cuts at: 0 398 679 1926
Size: 398 281 1247
Fragments arranged by size:
1247 398 281
SacI G_AGCT'C
Cuts at: 0 976 1926
Size: 976 950
SapI GCTCTTCn'nnn_
Cuts at: 0 635 1926
Size: 635 1291
ScaI AGT'ACT
Cuts at: 0 987 1926
Size: 987 939
SfcI C'TryA_G
Cuts at: 0 156 320 839 1115 1514 1808 1926
Size: 156 164 519 276 399 294 118
Fragments arranged by size:
519 399 294 276 164 156 118
SmlI C'TyrA_G
Cuts at: 0 511 1882 1926
Size: 511 1371 44
Fragments arranged by size:
1371 511 44
StyI C'CwwG_G
Cuts at: 0 520 1783 1926
Size: 520 1263 143
Fragments arranged by size:
1263 520 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 591 1644 1926
Size: 591 1053 282
Fragments arranged by size:
1053 591 282
TatI w'GTAC_w
Cuts at: 0 668 843 985 1233 1541 1926
Size: 668 175 142 248 308 385
Fragments arranged by size:
668 385 308 248 175 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1467 1499 1643 1675 1926
Size: 1467 32 144 32 251
Fragments arranged by size:
1467 251 144 32 32
XbaI T'CTAG_A
Cuts at: 0 379 1926
Size: 379 1547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1722 1827 1926
Size: 1722 105 99
Fragments arranged by size:
1722 105 99
XmnI GAAnn'nnTTC
Cuts at: 0 656 1142 1926
Size: 656 486 784
Fragments arranged by size:
784 656 486
Enzymes that do cut:
AarI AcuI AflII AhdI AloI AlwNI ApoI BanII
BbsI BbvCI BclI Bme1580I BmrI BmtI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrGI BstBI BstXI Bsu36I
BtsI DraIII EaeI EarI EcoICRI Eco57MI Hin4I HindIII
MmeI MscI MslI MspA1I NdeI NheI PmlI PpiI
PshAI PstI PvuII SacI SapI ScaI SfcI SmlI
StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BplI BsaI BsaHI BsaWI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI
BstAPI BstEII BstYI BstZ17I BtgI BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NgoMIV NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XhoI XmaI ZraI