Restriction map for
pF1KSDA0851
(Linear) (Six-base) MAPSORT of: KIAA0851.seq Check: 3023 from: 1 to: 1761
KIAA0851.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 177 1761
Size: 177 1584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 134 1207 1219 1761
Size: 46 88 1073 12 542
Fragments arranged by size:
1073 542 88 46 12
AhdI GACnn_n'nnGTC
Cuts at: 0 105 1761
Size: 105 1656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 977 1761
Size: 977 784
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1135 1167 1441 1473 1761
Size: 1135 32 274 32 288
Fragments arranged by size:
1135 288 274 32 32
ApaLI G'TGCA_C
Cuts at: 0 730 1761
Size: 730 1031
ApoI r'AATT_y
Cuts at: 0 49 247 454 1285 1558 1761
Size: 49 198 207 831 273 203
Fragments arranged by size:
831 273 207 203 198 49
AseI AT'TA_AT
Cuts at: 0 602 1761
Size: 602 1159
AvaI C'yCGr_G
Cuts at: 0 633 767 1761
Size: 633 134 994
Fragments arranged by size:
994 633 134
BanI G'GyrC_C
Cuts at: 0 1357 1761
Size: 1357 404
BanII G_rGCy'C
Cuts at: 0 937 1761
Size: 937 824
BbsI GAAGACnn'nnnn_
Cuts at: 0 310 968 1372 1761
Size: 310 658 404 389
Fragments arranged by size:
658 404 389 310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1145 1179 1761
Size: 1145 34 582
Fragments arranged by size:
1145 582 34
BfrBI ATG'CAT
Cuts at: 0 588 1761
Size: 588 1173
Bme1580I G_kGCm'C
Cuts at: 0 187 734 1761
Size: 187 547 1027
Fragments arranged by size:
1027 547 187
BmrI ACTGGGnnnn_n'
Cuts at: 0 190 1761
Size: 190 1571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 970 1761
Size: 793 177 791
Fragments arranged by size:
793 791 177
BsaBI GATnn'nnATC
Cuts at: 0 628 1761
Size: 628 1133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 630 660 1761
Size: 630 30 1101
Fragments arranged by size:
1101 630 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 651 654 1761
Size: 651 3 1107
Fragments arranged by size:
1107 651 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1761
Size: 515 1246
BsiHKAI G_wGCw'C
Cuts at: 0 734 1661 1761
Size: 734 927 100
Fragments arranged by size:
927 734 100
BsmI GAATG_Cn'
Cuts at: 0 588 1761
Size: 588 1173
Bsp1286I G_dGCh'C
Cuts at: 0 187 734 937 1661 1761
Size: 187 547 203 724 100
Fragments arranged by size:
724 547 203 187 100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 193 1761
Size: 193 1568
BsrDI GCAATG_nn'
Cuts at: 0 1004 1761
Size: 1004 757
BsrGI T'GTAC_A
Cuts at: 0 759 1761
Size: 759 1002
BstEII G'GTnAC_C
Cuts at: 0 117 1761
Size: 117 1644
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1553 1761
Size: 1553 208
BstZ17I GTA'TAC
Cuts at: 0 178 1761
Size: 178 1583
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 373 1761
Size: 373 1388
BtsI GCAGTG_nn'
Cuts at: 0 820 1761
Size: 820 941
EaeI y'GGCC_r
Cuts at: 0 1122 1761
Size: 1122 639
EarI CTCTTCn'nnn_
Cuts at: 0 1666 1761
Size: 1666 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 134 1207 1219 1761
Size: 46 88 1073 12 542
Fragments arranged by size:
1073 542 88 46 12
EcoRI G'AATT_C
Cuts at: 0 454 1761
Size: 454 1307
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1389 1470 1761
Size: 1389 81 291
Fragments arranged by size:
1389 291 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 791 823 1032 1064 1761
Size: 91 32 668 32 209 32 697
Fragments arranged by size:
697 668 209 91 32 32 32
HindIII A'AGCT_T
Cuts at: 0 62 749 1267 1761
Size: 62 687 518 494
Fragments arranged by size:
687 518 494 62
MfeI C'AATT_G
Cuts at: 0 1161 1761
Size: 1161 600
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1761
Size: 464 1297
MscI TGG'CCA
Cuts at: 0 1124 1761
Size: 1124 637
MslI CAynn'nnrTG
Cuts at: 0 737 1761
Size: 737 1024
MspA1I CmG'CkG
Cuts at: 0 24 154 432 492 1761
Size: 24 130 278 60 1269
Fragments arranged by size:
1269 278 130 60 24
NdeI CA'TA_TG
Cuts at: 0 408 1761
Size: 408 1353
NsiI A_TGCA'T
Cuts at: 0 590 1761
Size: 590 1171
NspI r_CATG'y
Cuts at: 0 1258 1761
Size: 1258 503
PflMI CCAn_nnn'nTGG
Cuts at: 0 172 198 1131 1761
Size: 172 26 933 630
Fragments arranged by size:
933 630 172 26
PsiI TTA'TAA
Cuts at: 0 299 910 1411 1445 1761
Size: 299 611 501 34 316
Fragments arranged by size:
611 501 316 299 34
PspXI vC'TCGA_Gb
Cuts at: 0 767 1761
Size: 767 994
PvuII CAG'CTG
Cuts at: 0 24 154 1761
Size: 24 130 1607
Fragments arranged by size:
1607 130 24
SapI GCTCTTCn'nnn_
Cuts at: 0 1666 1761
Size: 1666 95
SmlI C'TyrA_G
Cuts at: 0 633 767 808 949 1761
Size: 633 134 41 141 812
Fragments arranged by size:
812 633 141 134 41
SpeI A'CTAG_T
Cuts at: 0 445 1761
Size: 445 1316
SspI AAT'ATT
Cuts at: 0 170 1761
Size: 170 1591
StyI C'CwwG_G
Cuts at: 0 980 1546 1761
Size: 980 566 215
Fragments arranged by size:
980 566 215
TatI w'GTAC_w
Cuts at: 0 759 812 1761
Size: 759 53 949
Fragments arranged by size:
949 759 53
XbaI T'CTAG_A
Cuts at: 0 1176 1761
Size: 1176 585
XhoI C'TCGA_G
Cuts at: 0 633 767 1761
Size: 633 134 994
Fragments arranged by size:
994 633 134
XmnI GAAnn'nnTTC
Cuts at: 0 684 1503 1761
Size: 684 819 258
Fragments arranged by size:
819 684 258
Enzymes that do cut:
AccI AcuI AhdI AlfI AloI ApaLI ApoI AseI
AvaI BanI BanII BbsI BcgI BfrBI Bme1580I BmrI
BpuEI BsaBI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BsrGI BstEII BstXI BstZ17I BtgZI BtsI
EaeI EarI Eco57MI EcoRI FalI Hin4I HindIII MfeI
MmeI MscI MslI MspA1I NdeI NsiI NspI PflMI
PsiI PspXI PvuII SapI SmlI SpeI SspI StyI
TatI XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlwNI ApaI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BciVI BclI BglI BglII BlpI BmgBI
BmtI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI SwaI TaqII TstI Tth111I XcmI XmaI ZraI