Restriction map for pF1KSDA0863
 (Linear) (Six-base) MAPSORT of: KIAA0863.seq  Check: 9134  from: 1  to: 3393



KIAA0863.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2635    3393 
   Size:       2635     758
 
AccI GT'mk_AC

Cuts at:      0    2120    2516    2762    3393 
   Size:       2120     396     246     631

  Fragments arranged by size: 

                2120     631     396     246
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     354    2743    3091    3393 
   Size:        124     230    2389     348     302

  Fragments arranged by size: 

                2389     348     302     230     124
 
AfeI AGC'GCT

Cuts at:      0     852    3393 
   Size:        852    2541
 
AflIII A'CryG_T

Cuts at:      0     466    3393 
   Size:        466    2927
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3088    3393 
   Size:       3088     305
 
AleI CACnn'nnGTG

Cuts at:      0    2753    2873    3393 
   Size:       2753     120     520

  Fragments arranged by size: 

                2753     520     120
 
AlwNI CAG_nnn'CTG

Cuts at:      0     928    1929    3393 
   Size:        928    1001    1464

  Fragments arranged by size: 

                1464    1001     928
 
ApaLI G'TGCA_C

Cuts at:      0    2204    3393 
   Size:       2204    1189
 
ApoI r'AATT_y

Cuts at:      0       8    1614    3393 
   Size:          8    1606    1779

  Fragments arranged by size: 

                1779    1606       8
 
AvaI C'yCGr_G

Cuts at:      0    2599    3015    3393 
   Size:       2599     416     378

  Fragments arranged by size: 

                2599     416     378
 
BanI G'GyrC_C

Cuts at:      0     940    1714    2375    2556    2593    2690    3393 
   Size:        940     774     661     181      37      97     703

  Fragments arranged by size: 

                 940     774     703     661     181      97      37
 
BanII G_rGCy'C

Cuts at:      0    1267    2105    3393 
   Size:       1267     838    1288

  Fragments arranged by size: 

                1288    1267     838
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2086    2969    3393 
   Size:       2086     883     424

  Fragments arranged by size: 

                2086     883     424
 
BbvCI CC'TCA_GC

Cuts at:      0    1055    1584    2588    3393 
   Size:       1055     529    1004     805

  Fragments arranged by size: 

                1055    1004     805     529
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3024    3058    3393 
   Size:       3024      34     335

  Fragments arranged by size: 

                3024     335      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2531    3393 
   Size:       2531     862
 
BfrBI ATG'CAT

Cuts at:      0    2301    2907    3393 
   Size:       2301     606     486

  Fragments arranged by size: 

                2301     606     486
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1680    3393 
   Size:       1680    1713
 
BglII A'GATC_T

Cuts at:      0     742    3393 
   Size:        742    2651
 
Bme1580I G_kGCm'C

Cuts at:      0     945    1717    2208    2596    3393 
   Size:        945     772     491     388     797

  Fragments arranged by size: 

                 945     797     772     491     388
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     839     987    1358    1904    1970    3393 
   Size:        839     148     371     546      66    1423

  Fragments arranged by size: 

                1423     839     546     371     148      66
 
Bpu10I CC'TnA_GC

Cuts at:      0    1055    1584    2588    3393 
   Size:       1055     529    1004     805

  Fragments arranged by size: 

                1055    1004     805     529
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1140    2200    3393 
   Size:       1140    1060    1193

  Fragments arranged by size: 

                1193    1140    1060
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1005    1740    2271    3393 
   Size:       1005     735     531    1122

  Fragments arranged by size: 

                1122    1005     735     531
 
BsaBI GATnn'nnATC

Cuts at:      0    1918    3393 
   Size:       1918    1475
 
BsaWI w'CCGG_w

Cuts at:      0     392    3393 
   Size:        392    3001
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1887    1917    3393 
   Size:       1887      30    1476

  Fragments arranged by size: 

                1887    1476      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1569    1572    1973    2019    2022    2995    3393 
   Size:       1569       3     401      46       3     973     398

  Fragments arranged by size: 

                1569     973     401     398      46       3       3
 
BseYI C'CCAG_C

Cuts at:      0     906    1047    1211    2533    2852    3393 
   Size:        906     141     164    1322     319     541

  Fragments arranged by size: 

                1322     906     541     319     164     141
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     809    1931    1961    3393 
   Size:        809    1122      30    1432

  Fragments arranged by size: 

                1432    1122     809      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     846    2060    2105    2208    2363    2608    2823    3393 
   Size:        846    1214      45     103     155     245     215     570

  Fragments arranged by size: 

                1214     846     570     245     215     155     103      45
            
 
BsmI GAATG_Cn'

Cuts at:      0    2004    3393 
   Size:       2004    1389
 
Bsp1286I G_dGCh'C

Cuts at:      0     846     945    1267    1717    2060    2105    2208    2363 
   Size:        846      99     322     450     343      45     103     155

Cuts at:   2363    2596    2608    2823    3393 
   Size:        233      12     215     570

  Fragments arranged by size: 

                 846     570     450     343     322     233     215     155
                 103      99      45      12
 
BspEI T'CCGG_A

Cuts at:      0     392    3393 
   Size:        392    3001
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2092    2459    2635    2852    3393 
   Size:       2092     367     176     217     541

  Fragments arranged by size: 

                2092     541     367     217     176
 
BsrBI CCG'CTC

Cuts at:      0    2508    3393 
   Size:       2508     885
 
BsrDI GCAATG_nn'

Cuts at:      0     794    3393 
   Size:        794    2599
 
BsrFI r'CCGG_y

Cuts at:      0    1872    1957    2608    3393 
   Size:       1872      85     651     785

  Fragments arranged by size: 

                1872     785     651      85
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2203    2750    3393 
   Size:       2203     547     643

  Fragments arranged by size: 

                2203     643     547
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     357    2553    2755    2974    3393 
   Size:        357    2196     202     219     419

  Fragments arranged by size: 

                2196     419     357     219     202
 
BstYI r'GATC_y

Cuts at:      0     623     742    3123    3393 
   Size:        623     119    2381     270

  Fragments arranged by size: 

                2381     623     270     119
 
Bsu36I CC'TnA_GG

Cuts at:      0    2582    3393 
   Size:       2582     811
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     464    2256    2775    3393 
   Size:        464    1792     519     618

  Fragments arranged by size: 

                1792     618     519     464
 
BtsI GCAGTG_nn'

Cuts at:      0     846    2080    2748    3393 
   Size:        846    1234     668     645

  Fragments arranged by size: 

                1234     846     668     645
 
DraI TTT'AAA

Cuts at:      0    3051    3393 
   Size:       3051     342
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1479    3393 
   Size:       1479    1914
 
EaeI y'GGCC_r

Cuts at:      0     978    1434    1955    2946    3393 
   Size:        978     456     521     991     447

  Fragments arranged by size: 

                 991     978     521     456     447
 
EarI CTCTTCn'nnn_

Cuts at:      0    2270    3110    3393 
   Size:       2270     840     283

  Fragments arranged by size: 

                2270     840     283
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1895    3393 
   Size:       1895    1498
 
EcoICRI GAG'CTC

Cuts at:      0    2103    3393 
   Size:       2103    1290
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     354     839     987    1358    1904    1970    2743 
   Size:        124     230     485     148     371     546      66     773

Cuts at:   2743    3091    3393 
   Size:        348     302

  Fragments arranged by size: 

                 773     546     485     371     348     302     230     148
                 124      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0     108    1222    1284    3082    3393 
   Size:        108    1114      62    1798     311

  Fragments arranged by size: 

                1798    1114     311     108      62
 
EcoRI G'AATT_C

Cuts at:      0    1614    3393 
   Size:       1614    1779
 
EcoRV GAT'ATC

Cuts at:      0     202    2343    3393 
   Size:        202    2141    1050

  Fragments arranged by size: 

                2141    1050     202
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      82    3393 
   Size:         82    3311
 
HaeII r_GCGC'y

Cuts at:      0     854    3393 
   Size:        854    2539
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1790    1822    1887    1919    2149    2181    2821    2853 
   Size:       1790      32      65      32     230      32     640      32

Cuts at:   2853    3074    3106    3393 
   Size:        221      32     287

  Fragments arranged by size: 

                1790     640     287     230     221      65      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     736    2497    3393 
   Size:        736    1761     896

  Fragments arranged by size: 

                1761     896     736
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1113    1134    1861    2138    3393 
   Size:       1113      21     727     277    1255

  Fragments arranged by size: 

                1255    1113     727     277      21
 
MscI TGG'CCA

Cuts at:      0     980    1436    3393 
   Size:        980     456    1957

  Fragments arranged by size: 

                1957     980     456
 
MslI CAynn'nnrTG

Cuts at:      0     290    2753    2873    3393 
   Size:        290    2463     120     520

  Fragments arranged by size: 

                2463     520     290     120
 
MspA1I CmG'CkG

Cuts at:      0      89    1588    1945    2970    3393 
   Size:         89    1499     357    1025     423

  Fragments arranged by size: 

                1499    1025     423     357      89
 
NsiI A_TGCA'T

Cuts at:      0    2303    2909    3393 
   Size:       2303     606     484

  Fragments arranged by size: 

                2303     606     484
 
NspI r_CATG'y

Cuts at:      0     470     520    1977    3393 
   Size:        470      50    1457    1416

  Fragments arranged by size: 

                1457    1416     470      50
 
PacI TTA_AT'TAA

Cuts at:      0     548    3393 
   Size:        548    2845
 
PasI CC'CwG_GG

Cuts at:      0     936    1192    1279    1399    3393 
   Size:        936     256      87     120    1994

  Fragments arranged by size: 

                1994     936     256     120      87
 
PciI A'CATG_T

Cuts at:      0     466    3393 
   Size:        466    2927
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     309     835    1218    1710    2075    3393 
   Size:        309     526     383     492     365    1318

  Fragments arranged by size: 

                1318     526     492     383     365     309
 
PfoI T'CCnGG_A

Cuts at:      0     126    3393 
   Size:        126    3267
 
PpuMI rG'GwC_Cy

Cuts at:      0     108    1222    1284    3082    3393 
   Size:        108    1114      62    1798     311

  Fragments arranged by size: 

                1798    1114     311     108      62
 
PshAI GACnn'nnGTC

Cuts at:      0     997    3393 
   Size:        997    2396
 
PstI C_TGCA'G

Cuts at:      0    1043    1292    1353    1371    1389    1533    1631    2040 
   Size:       1043     249      61      18      18     144      98     409

Cuts at:   2040    2815    2849    3393 
   Size:        775      34     544

  Fragments arranged by size: 

                1043     775     544     409     249     144      98      61
                  34      18      18
 
PvuII CAG'CTG

Cuts at:      0      89    1588    1945    3393 
   Size:         89    1499     357    1448

  Fragments arranged by size: 

                1499    1448     357      89
 
SacI G_AGCT'C

Cuts at:      0    2105    3393 
   Size:       2105    1288
 
SanDI GG'GwC_CC

Cuts at:      0    1222    3393 
   Size:       1222    2171
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2270    3110    3393 
   Size:       2270     840     283

  Fragments arranged by size: 

                2270     840     283
 
SbfI CC_TGCA'GG

Cuts at:      0    1292    1353    1371    1389    2849    3393 
   Size:       1292      61      18      18    1460     544

  Fragments arranged by size: 

                1460    1292     544      61      18      18
 
SexAI A'CCwGG_T

Cuts at:      0    1749    2051    2123    3393 
   Size:       1749     302      72    1270

  Fragments arranged by size: 

                1749    1270     302      72
 
SfcI C'TryA_G

Cuts at:      0     501     614    1039    1288    1349    1367    1385    1529 
   Size:        501     113     425     249      61      18      18     144

Cuts at:   1529    1627    1811    2036    2811    2845    3393 
   Size:         98     184     225     775      34     548

  Fragments arranged by size: 

                 775     548     501     425     249     225     184     144
                 113      98      61      34      18      18
 
SmaI CCC'GGG

Cuts at:      0    2601    3017    3393 
   Size:       2601     416     376

  Fragments arranged by size: 

                2601     416     376
 
SmlI C'TyrA_G

Cuts at:      0    1119    2179    3393 
   Size:       1119    1060    1214

  Fragments arranged by size: 

                1214    1119    1060
 
SpeI A'CTAG_T

Cuts at:      0    1159    3393 
   Size:       1159    2234
 
SphI G_CATG'C

Cuts at:      0    1977    3393 
   Size:       1977    1416
 
SspI AAT'ATT

Cuts at:      0     420     643    3393 
   Size:        420     223    2750

  Fragments arranged by size: 

                2750     420     223
 
StuI AGG'CCT

Cuts at:      0    1683    1889    2026    2580    2665    3393 
   Size:       1683     206     137     554      85     728

  Fragments arranged by size: 

                1683     728     554     206     137      85
 
StyI C'CwwG_G

Cuts at:      0    2825    3393 
   Size:       2825     568
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     571    1225    3231    3393 
   Size:        571     654    2006     162

  Fragments arranged by size: 

                2006     654     571     162
 
TatI w'GTAC_w

Cuts at:      0     497    3324    3393 
   Size:        497    2827      69

  Fragments arranged by size: 

                2827     497      69
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     179     211     908     940    2143    2175    3393 
   Size:        179      32     697      32    1203      32    1218

  Fragments arranged by size: 

                1218    1203     697     179      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     943    2216    3393 
   Size:        943    1273    1177

  Fragments arranged by size: 

                1273    1177     943
 
XmaI C'CCGG_G

Cuts at:      0    2599    3015    3393 
   Size:       2599     416     378

  Fragments arranged by size: 

                2599     416     378
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AleI    AlwNI
    ApaLI     ApoI     AvaI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII Bme1580I     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI
    BstXI    BstYI   Bsu36I    BtgZI     BtsI     DraI     DrdI     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
    HaeII    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI    PpuMI    PshAI
     PstI    PvuII     SacI    SanDI     SapI     SbfI    SexAI     SfcI
     SmaI     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AlfI     AloI     ApaI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BclI
     BlpI    BmgBI     BmrI     BmtI     BplI    BsaAI    BsaHI    BsiEI
    BsiWI    BsmBI    BspHI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I     BtgI     ClaI    CspCI   DraIII     EagI    EcoNI     FseI
     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI     ScaI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SrfI     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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