Restriction map for
pF1KSDA0863
(Linear) (Six-base) MAPSORT of: KIAA0863.seq Check: 9134 from: 1 to: 3393
KIAA0863.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2635 3393
Size: 2635 758
AccI GT'mk_AC
Cuts at: 0 2120 2516 2762 3393
Size: 2120 396 246 631
Fragments arranged by size:
2120 631 396 246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 354 2743 3091 3393
Size: 124 230 2389 348 302
Fragments arranged by size:
2389 348 302 230 124
AfeI AGC'GCT
Cuts at: 0 852 3393
Size: 852 2541
AflIII A'CryG_T
Cuts at: 0 466 3393
Size: 466 2927
AhdI GACnn_n'nnGTC
Cuts at: 0 3088 3393
Size: 3088 305
AleI CACnn'nnGTG
Cuts at: 0 2753 2873 3393
Size: 2753 120 520
Fragments arranged by size:
2753 520 120
AlwNI CAG_nnn'CTG
Cuts at: 0 928 1929 3393
Size: 928 1001 1464
Fragments arranged by size:
1464 1001 928
ApaLI G'TGCA_C
Cuts at: 0 2204 3393
Size: 2204 1189
ApoI r'AATT_y
Cuts at: 0 8 1614 3393
Size: 8 1606 1779
Fragments arranged by size:
1779 1606 8
AvaI C'yCGr_G
Cuts at: 0 2599 3015 3393
Size: 2599 416 378
Fragments arranged by size:
2599 416 378
BanI G'GyrC_C
Cuts at: 0 940 1714 2375 2556 2593 2690 3393
Size: 940 774 661 181 37 97 703
Fragments arranged by size:
940 774 703 661 181 97 37
BanII G_rGCy'C
Cuts at: 0 1267 2105 3393
Size: 1267 838 1288
Fragments arranged by size:
1288 1267 838
BbsI GAAGACnn'nnnn_
Cuts at: 0 2086 2969 3393
Size: 2086 883 424
Fragments arranged by size:
2086 883 424
BbvCI CC'TCA_GC
Cuts at: 0 1055 1584 2588 3393
Size: 1055 529 1004 805
Fragments arranged by size:
1055 1004 805 529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3024 3058 3393
Size: 3024 34 335
Fragments arranged by size:
3024 335 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2531 3393
Size: 2531 862
BfrBI ATG'CAT
Cuts at: 0 2301 2907 3393
Size: 2301 606 486
Fragments arranged by size:
2301 606 486
BglI GCCn_nnn'nGGC
Cuts at: 0 1680 3393
Size: 1680 1713
BglII A'GATC_T
Cuts at: 0 742 3393
Size: 742 2651
Bme1580I G_kGCm'C
Cuts at: 0 945 1717 2208 2596 3393
Size: 945 772 491 388 797
Fragments arranged by size:
945 797 772 491 388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 987 1358 1904 1970 3393
Size: 839 148 371 546 66 1423
Fragments arranged by size:
1423 839 546 371 148 66
Bpu10I CC'TnA_GC
Cuts at: 0 1055 1584 2588 3393
Size: 1055 529 1004 805
Fragments arranged by size:
1055 1004 805 529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1140 2200 3393
Size: 1140 1060 1193
Fragments arranged by size:
1193 1140 1060
BsaI GGTCTCn'nnnn_
Cuts at: 0 1005 1740 2271 3393
Size: 1005 735 531 1122
Fragments arranged by size:
1122 1005 735 531
BsaBI GATnn'nnATC
Cuts at: 0 1918 3393
Size: 1918 1475
BsaWI w'CCGG_w
Cuts at: 0 392 3393
Size: 392 3001
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1887 1917 3393
Size: 1887 30 1476
Fragments arranged by size:
1887 1476 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1569 1572 1973 2019 2022 2995 3393
Size: 1569 3 401 46 3 973 398
Fragments arranged by size:
1569 973 401 398 46 3 3
BseYI C'CCAG_C
Cuts at: 0 906 1047 1211 2533 2852 3393
Size: 906 141 164 1322 319 541
Fragments arranged by size:
1322 906 541 319 164 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 1931 1961 3393
Size: 809 1122 30 1432
Fragments arranged by size:
1432 1122 809 30
BsiHKAI G_wGCw'C
Cuts at: 0 846 2060 2105 2208 2363 2608 2823 3393
Size: 846 1214 45 103 155 245 215 570
Fragments arranged by size:
1214 846 570 245 215 155 103 45
BsmI GAATG_Cn'
Cuts at: 0 2004 3393
Size: 2004 1389
Bsp1286I G_dGCh'C
Cuts at: 0 846 945 1267 1717 2060 2105 2208 2363
Size: 846 99 322 450 343 45 103 155
Cuts at: 2363 2596 2608 2823 3393
Size: 233 12 215 570
Fragments arranged by size:
846 570 450 343 322 233 215 155
103 99 45 12
BspEI T'CCGG_A
Cuts at: 0 392 3393
Size: 392 3001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2092 2459 2635 2852 3393
Size: 2092 367 176 217 541
Fragments arranged by size:
2092 541 367 217 176
BsrBI CCG'CTC
Cuts at: 0 2508 3393
Size: 2508 885
BsrDI GCAATG_nn'
Cuts at: 0 794 3393
Size: 794 2599
BsrFI r'CCGG_y
Cuts at: 0 1872 1957 2608 3393
Size: 1872 85 651 785
Fragments arranged by size:
1872 785 651 85
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2203 2750 3393
Size: 2203 547 643
Fragments arranged by size:
2203 643 547
BstXI CCAn_nnnn'nTGG
Cuts at: 0 357 2553 2755 2974 3393
Size: 357 2196 202 219 419
Fragments arranged by size:
2196 419 357 219 202
BstYI r'GATC_y
Cuts at: 0 623 742 3123 3393
Size: 623 119 2381 270
Fragments arranged by size:
2381 623 270 119
Bsu36I CC'TnA_GG
Cuts at: 0 2582 3393
Size: 2582 811
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 464 2256 2775 3393
Size: 464 1792 519 618
Fragments arranged by size:
1792 618 519 464
BtsI GCAGTG_nn'
Cuts at: 0 846 2080 2748 3393
Size: 846 1234 668 645
Fragments arranged by size:
1234 846 668 645
DraI TTT'AAA
Cuts at: 0 3051 3393
Size: 3051 342
DrdI GACnn_nn'nnGTC
Cuts at: 0 1479 3393
Size: 1479 1914
EaeI y'GGCC_r
Cuts at: 0 978 1434 1955 2946 3393
Size: 978 456 521 991 447
Fragments arranged by size:
991 978 521 456 447
EarI CTCTTCn'nnn_
Cuts at: 0 2270 3110 3393
Size: 2270 840 283
Fragments arranged by size:
2270 840 283
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1895 3393
Size: 1895 1498
EcoICRI GAG'CTC
Cuts at: 0 2103 3393
Size: 2103 1290
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 354 839 987 1358 1904 1970 2743
Size: 124 230 485 148 371 546 66 773
Cuts at: 2743 3091 3393
Size: 348 302
Fragments arranged by size:
773 546 485 371 348 302 230 148
124 66
EcoO109I rG'GnC_Cy
Cuts at: 0 108 1222 1284 3082 3393
Size: 108 1114 62 1798 311
Fragments arranged by size:
1798 1114 311 108 62
EcoRI G'AATT_C
Cuts at: 0 1614 3393
Size: 1614 1779
EcoRV GAT'ATC
Cuts at: 0 202 2343 3393
Size: 202 2141 1050
Fragments arranged by size:
2141 1050 202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 82 3393
Size: 82 3311
HaeII r_GCGC'y
Cuts at: 0 854 3393
Size: 854 2539
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1790 1822 1887 1919 2149 2181 2821 2853
Size: 1790 32 65 32 230 32 640 32
Cuts at: 2853 3074 3106 3393
Size: 221 32 287
Fragments arranged by size:
1790 640 287 230 221 65 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 736 2497 3393
Size: 736 1761 896
Fragments arranged by size:
1761 896 736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1113 1134 1861 2138 3393
Size: 1113 21 727 277 1255
Fragments arranged by size:
1255 1113 727 277 21
MscI TGG'CCA
Cuts at: 0 980 1436 3393
Size: 980 456 1957
Fragments arranged by size:
1957 980 456
MslI CAynn'nnrTG
Cuts at: 0 290 2753 2873 3393
Size: 290 2463 120 520
Fragments arranged by size:
2463 520 290 120
MspA1I CmG'CkG
Cuts at: 0 89 1588 1945 2970 3393
Size: 89 1499 357 1025 423
Fragments arranged by size:
1499 1025 423 357 89
NsiI A_TGCA'T
Cuts at: 0 2303 2909 3393
Size: 2303 606 484
Fragments arranged by size:
2303 606 484
NspI r_CATG'y
Cuts at: 0 470 520 1977 3393
Size: 470 50 1457 1416
Fragments arranged by size:
1457 1416 470 50
PacI TTA_AT'TAA
Cuts at: 0 548 3393
Size: 548 2845
PasI CC'CwG_GG
Cuts at: 0 936 1192 1279 1399 3393
Size: 936 256 87 120 1994
Fragments arranged by size:
1994 936 256 120 87
PciI A'CATG_T
Cuts at: 0 466 3393
Size: 466 2927
PflMI CCAn_nnn'nTGG
Cuts at: 0 309 835 1218 1710 2075 3393
Size: 309 526 383 492 365 1318
Fragments arranged by size:
1318 526 492 383 365 309
PfoI T'CCnGG_A
Cuts at: 0 126 3393
Size: 126 3267
PpuMI rG'GwC_Cy
Cuts at: 0 108 1222 1284 3082 3393
Size: 108 1114 62 1798 311
Fragments arranged by size:
1798 1114 311 108 62
PshAI GACnn'nnGTC
Cuts at: 0 997 3393
Size: 997 2396
PstI C_TGCA'G
Cuts at: 0 1043 1292 1353 1371 1389 1533 1631 2040
Size: 1043 249 61 18 18 144 98 409
Cuts at: 2040 2815 2849 3393
Size: 775 34 544
Fragments arranged by size:
1043 775 544 409 249 144 98 61
34 18 18
PvuII CAG'CTG
Cuts at: 0 89 1588 1945 3393
Size: 89 1499 357 1448
Fragments arranged by size:
1499 1448 357 89
SacI G_AGCT'C
Cuts at: 0 2105 3393
Size: 2105 1288
SanDI GG'GwC_CC
Cuts at: 0 1222 3393
Size: 1222 2171
SapI GCTCTTCn'nnn_
Cuts at: 0 2270 3110 3393
Size: 2270 840 283
Fragments arranged by size:
2270 840 283
SbfI CC_TGCA'GG
Cuts at: 0 1292 1353 1371 1389 2849 3393
Size: 1292 61 18 18 1460 544
Fragments arranged by size:
1460 1292 544 61 18 18
SexAI A'CCwGG_T
Cuts at: 0 1749 2051 2123 3393
Size: 1749 302 72 1270
Fragments arranged by size:
1749 1270 302 72
SfcI C'TryA_G
Cuts at: 0 501 614 1039 1288 1349 1367 1385 1529
Size: 501 113 425 249 61 18 18 144
Cuts at: 1529 1627 1811 2036 2811 2845 3393
Size: 98 184 225 775 34 548
Fragments arranged by size:
775 548 501 425 249 225 184 144
113 98 61 34 18 18
SmaI CCC'GGG
Cuts at: 0 2601 3017 3393
Size: 2601 416 376
Fragments arranged by size:
2601 416 376
SmlI C'TyrA_G
Cuts at: 0 1119 2179 3393
Size: 1119 1060 1214
Fragments arranged by size:
1214 1119 1060
SpeI A'CTAG_T
Cuts at: 0 1159 3393
Size: 1159 2234
SphI G_CATG'C
Cuts at: 0 1977 3393
Size: 1977 1416
SspI AAT'ATT
Cuts at: 0 420 643 3393
Size: 420 223 2750
Fragments arranged by size:
2750 420 223
StuI AGG'CCT
Cuts at: 0 1683 1889 2026 2580 2665 3393
Size: 1683 206 137 554 85 728
Fragments arranged by size:
1683 728 554 206 137 85
StyI C'CwwG_G
Cuts at: 0 2825 3393
Size: 2825 568
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 571 1225 3231 3393
Size: 571 654 2006 162
Fragments arranged by size:
2006 654 571 162
TatI w'GTAC_w
Cuts at: 0 497 3324 3393
Size: 497 2827 69
Fragments arranged by size:
2827 497 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 908 940 2143 2175 3393
Size: 179 32 697 32 1203 32 1218
Fragments arranged by size:
1218 1203 697 179 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 943 2216 3393
Size: 943 1273 1177
Fragments arranged by size:
1273 1177 943
XmaI C'CCGG_G
Cuts at: 0 2599 3015 3393
Size: 2599 416 378
Fragments arranged by size:
2599 416 378
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlwNI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI
BciVI BfrBI BglI BglII Bme1580I BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HindIII MmeI MscI MslI MspA1I NsiI
NspI PacI PasI PciI PflMI PfoI PpuMI PshAI
PstI PvuII SacI SanDI SapI SbfI SexAI SfcI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AlfI AloI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BclI
BlpI BmgBI BmrI BmtI BplI BsaAI BsaHI BsiEI
BsiWI BsmBI BspHI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtgI ClaI CspCI DraIII EagI EcoNI FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI ScaI SfiI SfoI SgfI SgrAI
SnaBI SrfI SwaI Tth111I XbaI XhoI XmnI ZraI