(Linear) (Six-base) MAPSORT of: KIAA0863.seq Check: 9134 from: 1 to: 3393 KIAA0863.orf With 182 enzymes: SgfI * October 24, 2006 15:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2635 3393 Size: 2635 758 AccI GT'mk_AC Cuts at: 0 2120 2516 2762 3393 Size: 2120 396 246 631 Fragments arranged by size: 2120 631 396 246 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 354 2743 3091 3393 Size: 124 230 2389 348 302 Fragments arranged by size: 2389 348 302 230 124 AfeI AGC'GCT Cuts at: 0 852 3393 Size: 852 2541 AflIII A'CryG_T Cuts at: 0 466 3393 Size: 466 2927 AhdI GACnn_n'nnGTC Cuts at: 0 3088 3393 Size: 3088 305 AleI CACnn'nnGTG Cuts at: 0 2753 2873 3393 Size: 2753 120 520 Fragments arranged by size: 2753 520 120 AlwNI CAG_nnn'CTG Cuts at: 0 928 1929 3393 Size: 928 1001 1464 Fragments arranged by size: 1464 1001 928 ApaLI G'TGCA_C Cuts at: 0 2204 3393 Size: 2204 1189 ApoI r'AATT_y Cuts at: 0 8 1614 3393 Size: 8 1606 1779 Fragments arranged by size: 1779 1606 8 AvaI C'yCGr_G Cuts at: 0 2599 3015 3393 Size: 2599 416 378 Fragments arranged by size: 2599 416 378 BanI G'GyrC_C Cuts at: 0 940 1714 2375 2556 2593 2690 3393 Size: 940 774 661 181 37 97 703 Fragments arranged by size: 940 774 703 661 181 97 37 BanII G_rGCy'C Cuts at: 0 1267 2105 3393 Size: 1267 838 1288 Fragments arranged by size: 1288 1267 838 BbsI GAAGACnn'nnnn_ Cuts at: 0 2086 2969 3393 Size: 2086 883 424 Fragments arranged by size: 2086 883 424 BbvCI CC'TCA_GC Cuts at: 0 1055 1584 2588 3393 Size: 1055 529 1004 805 Fragments arranged by size: 1055 1004 805 529 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3024 3058 3393 Size: 3024 34 335 Fragments arranged by size: 3024 335 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2531 3393 Size: 2531 862 BfrBI ATG'CAT Cuts at: 0 2301 2907 3393 Size: 2301 606 486 Fragments arranged by size: 2301 606 486 BglI GCCn_nnn'nGGC Cuts at: 0 1680 3393 Size: 1680 1713 BglII A'GATC_T Cuts at: 0 742 3393 Size: 742 2651 Bme1580I G_kGCm'C Cuts at: 0 945 1717 2208 2596 3393 Size: 945 772 491 388 797 Fragments arranged by size: 945 797 772 491 388 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 839 987 1358 1904 1970 3393 Size: 839 148 371 546 66 1423 Fragments arranged by size: 1423 839 546 371 148 66 Bpu10I CC'TnA_GC Cuts at: 0 1055 1584 2588 3393 Size: 1055 529 1004 805 Fragments arranged by size: 1055 1004 805 529 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1140 2200 3393 Size: 1140 1060 1193 Fragments arranged by size: 1193 1140 1060 BsaI GGTCTCn'nnnn_ Cuts at: 0 1005 1740 2271 3393 Size: 1005 735 531 1122 Fragments arranged by size: 1122 1005 735 531 BsaBI GATnn'nnATC Cuts at: 0 1918 3393 Size: 1918 1475 BsaWI w'CCGG_w Cuts at: 0 392 3393 Size: 392 3001 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1887 1917 3393 Size: 1887 30 1476 Fragments arranged by size: 1887 1476 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1569 1572 1973 2019 2022 2995 3393 Size: 1569 3 401 46 3 973 398 Fragments arranged by size: 1569 973 401 398 46 3 3 BseYI C'CCAG_C Cuts at: 0 906 1047 1211 2533 2852 3393 Size: 906 141 164 1322 319 541 Fragments arranged by size: 1322 906 541 319 164 141 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 809 1931 1961 3393 Size: 809 1122 30 1432 Fragments arranged by size: 1432 1122 809 30 BsiHKAI G_wGCw'C Cuts at: 0 846 2060 2105 2208 2363 2608 2823 3393 Size: 846 1214 45 103 155 245 215 570 Fragments arranged by size: 1214 846 570 245 215 155 103 45 BsmI GAATG_Cn' Cuts at: 0 2004 3393 Size: 2004 1389 Bsp1286I G_dGCh'C Cuts at: 0 846 945 1267 1717 2060 2105 2208 2363 Size: 846 99 322 450 343 45 103 155 Cuts at: 2363 2596 2608 2823 3393 Size: 233 12 215 570 Fragments arranged by size: 846 570 450 343 322 233 215 155 103 99 45 12 BspEI T'CCGG_A Cuts at: 0 392 3393 Size: 392 3001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2092 2459 2635 2852 3393 Size: 2092 367 176 217 541 Fragments arranged by size: 2092 541 367 217 176 BsrBI CCG'CTC Cuts at: 0 2508 3393 Size: 2508 885 BsrDI GCAATG_nn' Cuts at: 0 794 3393 Size: 794 2599 BsrFI r'CCGG_y Cuts at: 0 1872 1957 2608 3393 Size: 1872 85 651 785 Fragments arranged by size: 1872 785 651 85 BstAPI GCAn_nnn'nTGC Cuts at: 0 2203 2750 3393 Size: 2203 547 643 Fragments arranged by size: 2203 643 547 BstXI CCAn_nnnn'nTGG Cuts at: 0 357 2553 2755 2974 3393 Size: 357 2196 202 219 419 Fragments arranged by size: 2196 419 357 219 202 BstYI r'GATC_y Cuts at: 0 623 742 3123 3393 Size: 623 119 2381 270 Fragments arranged by size: 2381 623 270 119 Bsu36I CC'TnA_GG Cuts at: 0 2582 3393 Size: 2582 811 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 464 2256 2775 3393 Size: 464 1792 519 618 Fragments arranged by size: 1792 618 519 464 BtsI GCAGTG_nn' Cuts at: 0 846 2080 2748 3393 Size: 846 1234 668 645 Fragments arranged by size: 1234 846 668 645 DraI TTT'AAA Cuts at: 0 3051 3393 Size: 3051 342 DrdI GACnn_nn'nnGTC Cuts at: 0 1479 3393 Size: 1479 1914 EaeI y'GGCC_r Cuts at: 0 978 1434 1955 2946 3393 Size: 978 456 521 991 447 Fragments arranged by size: 991 978 521 456 447 EarI CTCTTCn'nnn_ Cuts at: 0 2270 3110 3393 Size: 2270 840 283 Fragments arranged by size: 2270 840 283 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1895 3393 Size: 1895 1498 EcoICRI GAG'CTC Cuts at: 0 2103 3393 Size: 2103 1290 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 354 839 987 1358 1904 1970 2743 Size: 124 230 485 148 371 546 66 773 Cuts at: 2743 3091 3393 Size: 348 302 Fragments arranged by size: 773 546 485 371 348 302 230 148 124 66 EcoO109I rG'GnC_Cy Cuts at: 0 108 1222 1284 3082 3393 Size: 108 1114 62 1798 311 Fragments arranged by size: 1798 1114 311 108 62 EcoRI G'AATT_C Cuts at: 0 1614 3393 Size: 1614 1779 EcoRV GAT'ATC Cuts at: 0 202 2343 3393 Size: 202 2141 1050 Fragments arranged by size: 2141 1050 202 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 82 3393 Size: 82 3311 HaeII r_GCGC'y Cuts at: 0 854 3393 Size: 854 2539 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1790 1822 1887 1919 2149 2181 2821 2853 Size: 1790 32 65 32 230 32 640 32 Cuts at: 2853 3074 3106 3393 Size: 221 32 287 Fragments arranged by size: 1790 640 287 230 221 65 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 736 2497 3393 Size: 736 1761 896 Fragments arranged by size: 1761 896 736 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1113 1134 1861 2138 3393 Size: 1113 21 727 277 1255 Fragments arranged by size: 1255 1113 727 277 21 MscI TGG'CCA Cuts at: 0 980 1436 3393 Size: 980 456 1957 Fragments arranged by size: 1957 980 456 MslI CAynn'nnrTG Cuts at: 0 290 2753 2873 3393 Size: 290 2463 120 520 Fragments arranged by size: 2463 520 290 120 MspA1I CmG'CkG Cuts at: 0 89 1588 1945 2970 3393 Size: 89 1499 357 1025 423 Fragments arranged by size: 1499 1025 423 357 89 NsiI A_TGCA'T Cuts at: 0 2303 2909 3393 Size: 2303 606 484 Fragments arranged by size: 2303 606 484 NspI r_CATG'y Cuts at: 0 470 520 1977 3393 Size: 470 50 1457 1416 Fragments arranged by size: 1457 1416 470 50 PacI TTA_AT'TAA Cuts at: 0 548 3393 Size: 548 2845 PasI CC'CwG_GG Cuts at: 0 936 1192 1279 1399 3393 Size: 936 256 87 120 1994 Fragments arranged by size: 1994 936 256 120 87 PciI A'CATG_T Cuts at: 0 466 3393 Size: 466 2927 PflMI CCAn_nnn'nTGG Cuts at: 0 309 835 1218 1710 2075 3393 Size: 309 526 383 492 365 1318 Fragments arranged by size: 1318 526 492 383 365 309 PfoI T'CCnGG_A Cuts at: 0 126 3393 Size: 126 3267 PpuMI rG'GwC_Cy Cuts at: 0 108 1222 1284 3082 3393 Size: 108 1114 62 1798 311 Fragments arranged by size: 1798 1114 311 108 62 PshAI GACnn'nnGTC Cuts at: 0 997 3393 Size: 997 2396 PstI C_TGCA'G Cuts at: 0 1043 1292 1353 1371 1389 1533 1631 2040 Size: 1043 249 61 18 18 144 98 409 Cuts at: 2040 2815 2849 3393 Size: 775 34 544 Fragments arranged by size: 1043 775 544 409 249 144 98 61 34 18 18 PvuII CAG'CTG Cuts at: 0 89 1588 1945 3393 Size: 89 1499 357 1448 Fragments arranged by size: 1499 1448 357 89 SacI G_AGCT'C Cuts at: 0 2105 3393 Size: 2105 1288 SanDI GG'GwC_CC Cuts at: 0 1222 3393 Size: 1222 2171 SapI GCTCTTCn'nnn_ Cuts at: 0 2270 3110 3393 Size: 2270 840 283 Fragments arranged by size: 2270 840 283 SbfI CC_TGCA'GG Cuts at: 0 1292 1353 1371 1389 2849 3393 Size: 1292 61 18 18 1460 544 Fragments arranged by size: 1460 1292 544 61 18 18 SexAI A'CCwGG_T Cuts at: 0 1749 2051 2123 3393 Size: 1749 302 72 1270 Fragments arranged by size: 1749 1270 302 72 SfcI C'TryA_G Cuts at: 0 501 614 1039 1288 1349 1367 1385 1529 Size: 501 113 425 249 61 18 18 144 Cuts at: 1529 1627 1811 2036 2811 2845 3393 Size: 98 184 225 775 34 548 Fragments arranged by size: 775 548 501 425 249 225 184 144 113 98 61 34 18 18 SmaI CCC'GGG Cuts at: 0 2601 3017 3393 Size: 2601 416 376 Fragments arranged by size: 2601 416 376 SmlI C'TyrA_G Cuts at: 0 1119 2179 3393 Size: 1119 1060 1214 Fragments arranged by size: 1214 1119 1060 SpeI A'CTAG_T Cuts at: 0 1159 3393 Size: 1159 2234 SphI G_CATG'C Cuts at: 0 1977 3393 Size: 1977 1416 SspI AAT'ATT Cuts at: 0 420 643 3393 Size: 420 223 2750 Fragments arranged by size: 2750 420 223 StuI AGG'CCT Cuts at: 0 1683 1889 2026 2580 2665 3393 Size: 1683 206 137 554 85 728 Fragments arranged by size: 1683 728 554 206 137 85 StyI C'CwwG_G Cuts at: 0 2825 3393 Size: 2825 568 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 571 1225 3231 3393 Size: 571 654 2006 162 Fragments arranged by size: 2006 654 571 162 TatI w'GTAC_w Cuts at: 0 497 3324 3393 Size: 497 2827 69 Fragments arranged by size: 2827 497 69 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 179 211 908 940 2143 2175 3393 Size: 179 32 697 32 1203 32 1218 Fragments arranged by size: 1218 1203 697 179 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 943 2216 3393 Size: 943 1273 1177 Fragments arranged by size: 1273 1177 943 XmaI C'CCGG_G Cuts at: 0 2599 3015 3393 Size: 2599 416 378 Fragments arranged by size: 2599 416 378 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AhdI AleI AlwNI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NsiI NspI PacI PasI PciI PflMI PfoI PpuMI PshAI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BclI BlpI BmgBI BmrI BmtI BplI BsaAI BsaHI BsiEI BsiWI BsmBI BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgI ClaI CspCI DraIII EagI EcoNI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI ScaI SfiI SfoI SgfI SgrAI SnaBI SrfI SwaI Tth111I XbaI XhoI XmnI ZraI