Restriction map for
pF1KA0866
(Linear) (Six-base) MAPSORT of: KIAA0866.seq Check: 5342 from: 1 to: 5916
KIAA0866.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 398 2526 3768 5559 5916
Size: 398 2128 1242 1791 357
Fragments arranged by size:
2128 1791 1242 398 357
AatII G_ACGT'C
Cuts at: 0 755 5916
Size: 755 5161
AccI GT'mk_AC
Cuts at: 0 416 4003 5916
Size: 416 3587 1913
Fragments arranged by size:
3587 1913 416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3349 3556 4258 4876 5551 5572 5916
Size: 3349 207 702 618 675 21 344
Fragments arranged by size:
3349 702 675 618 344 207 21
AflII C'TTAA_G
Cuts at: 0 2623 5916
Size: 2623 3293
AflIII A'CryG_T
Cuts at: 0 419 3998 5916
Size: 419 3579 1918
Fragments arranged by size:
3579 1918 419
AhdI GACnn_n'nnGTC
Cuts at: 0 1107 3930 5916
Size: 1107 2823 1986
Fragments arranged by size:
2823 1986 1107
AleI CACnn'nnGTG
Cuts at: 0 1315 2518 5916
Size: 1315 1203 3398
Fragments arranged by size:
3398 1315 1203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2125 4163 5722 5916
Size: 2125 2038 1559 194
Fragments arranged by size:
2125 2038 1559 194
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 697 729 1603 1635 2990 3022 5916
Size: 697 32 874 32 1355 32 2894
Fragments arranged by size:
2894 1355 874 697 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 582 2436 2716 2757 2856 2904 3156 3684
Size: 582 1854 280 41 99 48 252 528
Cuts at: 3684 3789 3966 4083 4284 5916
Size: 105 177 117 201 1632
Fragments arranged by size:
1854 1632 582 528 280 252 201 177
117 105 99 48 41
ApaI G_GGCC'C
Cuts at: 0 3822 4473 4608 5916
Size: 3822 651 135 1308
Fragments arranged by size:
3822 1308 651 135
ApaLI G'TGCA_C
Cuts at: 0 1659 5916
Size: 1659 4257
ApoI r'AATT_y
Cuts at: 0 730 4354 5916
Size: 730 3624 1562
Fragments arranged by size:
3624 1562 730
AseI AT'TA_AT
Cuts at: 0 1160 5916
Size: 1160 4756
AvaI C'yCGr_G
Cuts at: 0 3821 4466 4504 4745 5122 5368 5879 5916
Size: 3821 645 38 241 377 246 511 37
Fragments arranged by size:
3821 645 511 377 246 241 38 37
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 609 642 5916
Size: 609 33 5274
Fragments arranged by size:
5274 609 33
BamHI G'GATC_C
Cuts at: 0 1367 5916
Size: 1367 4549
BanII G_rGCy'C
Cuts at: 0 1562 1912 3242 3503 3623 3822 3833 4473
Size: 1562 350 1330 261 120 199 11 640
Cuts at: 4473 4608 5106 5390 5916
Size: 135 498 284 526
Fragments arranged by size:
1562 1330 640 526 498 350 284 261
199 135 120 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 1071 2151 2593 2810 3473 4547 4639 4823
Size: 1071 1080 442 217 663 1074 92 184
Cuts at: 4823 5916
Size: 1093
Fragments arranged by size:
1093 1080 1074 1071 663 442 217 184
92
BbvCI CC'TCA_GC
Cuts at: 0 2717 2927 4421 5916
Size: 2717 210 1494 1495
Fragments arranged by size:
2717 1495 1494 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 729 763 5916
Size: 729 34 5153
Fragments arranged by size:
5153 729 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1298 5916
Size: 1298 4618
BclI T'GATC_A
Cuts at: 0 2249 2973 4359 5916
Size: 2249 724 1386 1557
Fragments arranged by size:
2249 1557 1386 724
BglI GCCn_nnn'nGGC
Cuts at: 0 592 2138 2380 2410 5916
Size: 592 1546 242 30 3506
Fragments arranged by size:
3506 1546 592 242 30
BglII A'GATC_T
Cuts at: 0 1391 5009 5916
Size: 1391 3618 907
Fragments arranged by size:
3618 1391 907
BlpI GC'TnA_GC
Cuts at: 0 5305 5916
Size: 5305 611
Bme1580I G_kGCm'C
Cuts at: 0 947 1663 3822 4473 4608 4839 5358 5916
Size: 947 716 2159 651 135 231 519 558
Fragments arranged by size:
2159 947 716 651 558 519 231 135
BmgBI CAC'GTC
Cuts at: 0 281 1863 3927 4566 5916
Size: 281 1582 2064 639 1350
Fragments arranged by size:
2064 1582 1350 639 281
BmrI ACTGGGnnnn_n'
Cuts at: 0 64 96 5916
Size: 64 32 5820
Fragments arranged by size:
5820 64 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1422 5916
Size: 1422 4494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 694 887 1501 1568 2107 2779 2812
Size: 560 134 193 614 67 539 672 33
Cuts at: 2812 2896 2968 3178 3199 3205 3352 3367 3463
Size: 84 72 210 21 6 147 15 96
Cuts at: 3463 3706 3790 3934 4042 4105 4609 4630 4672
Size: 243 84 144 108 63 504 21 42
Cuts at: 4672 4906 4983 5164 5221 5242 5254 5476 5677
Size: 234 77 181 57 21 12 222 201
Cuts at: 5677 5846 5916
Size: 169 70
Fragments arranged by size:
672 614 560 539 504 243 234 222
210 201 193 181 169 147 144 134
108 96 84 84 77 72 70 67
63 57 42 33 21 21 21 15
12 6
Bpu10I CC'TnA_GC
Cuts at: 0 2717 2927 3502 3575 4421 5782 5916
Size: 2717 210 575 73 846 1361 134
Fragments arranged by size:
2717 1361 846 575 210 134 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1690 2464 2932 3031 3226 3655 4699 5629
Size: 1690 774 468 99 195 429 1044 930
Cuts at: 5629 5755 5916
Size: 126 161
Fragments arranged by size:
1690 1044 930 774 468 429 195 161
126 99
BsaI GGTCTCn'nnnn_
Cuts at: 0 778 3419 4606 5791 5916
Size: 778 2641 1187 1185 125
Fragments arranged by size:
2641 1187 1185 778 125
BsaAI yAC'GTr
Cuts at: 0 347 5916
Size: 347 5569
BsaHI Gr'CG_yC
Cuts at: 0 752 1108 2318 3931 4679 5916
Size: 752 356 1210 1613 748 1237
Fragments arranged by size:
1613 1237 1210 752 748 356
BsaWI w'CCGG_w
Cuts at: 0 485 2150 5390 5916
Size: 485 1665 3240 526
Fragments arranged by size:
3240 1665 526 485
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 280 295 310 499 529 697 727
Size: 265 15 15 15 189 30 168 30
Cuts at: 727 1642 1672 2990 3020 3778 3808 5916
Size: 915 30 1318 30 758 30 2108
Fragments arranged by size:
2108 1318 915 758 265 189 168 30
30 30 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 588 705 1033 1036 1184 1504 1818
Size: 169 419 117 328 3 148 320 314
Cuts at: 1818 2350 2353 2566 2569 2674 2734 2776 2809
Size: 532 3 213 3 105 60 42 33
Cuts at: 2809 2812 2893 2896 3028 3280 3283 3451 3631
Size: 3 81 3 132 252 3 168 180
Cuts at: 3631 4039 4042 4075 4240 4390 4639 4777 4785
Size: 408 3 33 165 150 249 138 8
Cuts at: 4785 5158 5239 5242 5251 5254 5494 5674 5683
Size: 373 81 3 9 3 240 180 9
Cuts at: 5683 5686 5878 5916
Size: 3 192 38
Fragments arranged by size:
532 419 408 373 328 320 314 252
249 240 213 192 180 180 169 168
165 150 148 138 132 117 105 81
81 60 42 38 33 33 9 9
8 3 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 951 1741 1918 1980 2846 3944 4214 5216
Size: 951 790 177 62 866 1098 270 1002
Cuts at: 5216 5678 5916
Size: 462 238
Fragments arranged by size:
1098 1002 951 866 790 462 270 238
177 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3802 3822 5916
Size: 3802 20 2094
Fragments arranged by size:
3802 2094 20
BsiEI CG_ry'CG
Cuts at: 0 1573 4238 5271 5916
Size: 1573 2665 1033 645
Fragments arranged by size:
2665 1573 1033 645
BsiHKAI G_wGCw'C
Cuts at: 0 1562 1663 1912 3242 3503 3623 3833 5390
Size: 1562 101 249 1330 261 120 210 1557
Cuts at: 5390 5916
Size: 526
Fragments arranged by size:
1562 1557 1330 526 261 249 210 120
101
BsmI GAATG_Cn'
Cuts at: 0 1618 2120 2205 2240 5916
Size: 1618 502 85 35 3676
Fragments arranged by size:
3676 1618 502 85 35
BsmBI CGTCTCn'nnnn_
Cuts at: 0 821 3550 4798 5876 5916
Size: 821 2729 1248 1078 40
Fragments arranged by size:
2729 1248 1078 821 40
Bsp1286I G_dGCh'C
Cuts at: 0 947 1562 1663 1912 3242 3503 3623 3822
Size: 947 615 101 249 1330 261 120 199
Cuts at: 3822 3833 4473 4608 4839 5106 5358 5390 5916
Size: 11 640 135 231 267 252 32 526
Fragments arranged by size:
1330 947 640 615 526 267 261 252
249 231 199 135 120 101 32 11
BspEI T'CCGG_A
Cuts at: 0 5390 5916
Size: 5390 526
BspHI T'CATG_A
Cuts at: 0 2246 5916
Size: 2246 3670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 398 2526 2678 3768 5475 5559 5916
Size: 398 2128 152 1090 1707 84 357
Fragments arranged by size:
2128 1707 1090 398 357 152 84
BsrBI CCG'CTC
Cuts at: 0 321 2598 3390 3816 4032 4509 5373 5916
Size: 321 2277 792 426 216 477 864 543
Fragments arranged by size:
2277 864 792 543 477 426 321 216
BsrDI GCAATG_nn'
Cuts at: 0 2109 5631 5916
Size: 2109 3522 285
Fragments arranged by size:
3522 2109 285
BsrFI r'CCGG_y
Cuts at: 0 2132 5916
Size: 2132 3784
BsrGI T'GTAC_A
Cuts at: 0 422 1967 5916
Size: 422 1545 3949
Fragments arranged by size:
3949 1545 422
BssSI C'ACGA_G
Cuts at: 0 442 2047 3202 4324 4795 5404 5916
Size: 442 1605 1155 1122 471 609 512
Fragments arranged by size:
1605 1155 1122 609 512 471 442
BstAPI GCAn_nnn'nTGC
Cuts at: 0 447 2904 3789 5916
Size: 447 2457 885 2127
Fragments arranged by size:
2457 2127 885 447
BstBI TT'CG_AA
Cuts at: 0 4727 5916
Size: 4727 1189
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1741 1785 3915 3927 4158 5052 5916
Size: 1741 44 2130 12 231 894 864
Fragments arranged by size:
2130 1741 894 864 231 44 12
BstYI r'GATC_y
Cuts at: 0 1180 1367 1391 2186 2957 3335 4734 5009
Size: 1180 187 24 795 771 378 1399 275
Cuts at: 5009 5916
Size: 907
Fragments arranged by size:
1399 1180 907 795 771 378 275 187
24
Bsu36I CC'TnA_GG
Cuts at: 0 313 5916
Size: 313 5603
BtgI C'CryG_G
Cuts at: 0 587 986 995 1631 4157 4670 5729 5747
Size: 587 399 9 636 2526 513 1059 18
Cuts at: 5747 5916
Size: 169
Fragments arranged by size:
2526 1059 636 587 513 399 169 18
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 453 2398 3821 5430 5916
Size: 453 1945 1423 1609 486
Fragments arranged by size:
1945 1609 1423 486 453
BtsI GCAGTG_nn'
Cuts at: 0 2503 2527 5916
Size: 2503 24 3389
Fragments arranged by size:
3389 2503 24
EaeI y'GGCC_r
Cuts at: 0 584 1271 1844 1892 2402 2741 3254 3287
Size: 584 687 573 48 510 339 513 33
Cuts at: 3287 3707 3917 4235 5150 5318 5516 5591 5916
Size: 420 210 318 915 168 198 75 325
Fragments arranged by size:
915 687 584 573 513 510 420 339
325 318 210 198 168 75 48 33
EagI C'GGCC_G
Cuts at: 0 4235 5916
Size: 4235 1681
EarI CTCTTCn'nnn_
Cuts at: 0 364 430 1462 2047 3044 3131 3136 3548
Size: 364 66 1032 585 997 87 5 412
Cuts at: 3548 4166 4177 4395 4472 4640 4762 5916
Size: 618 11 218 77 168 122 1154
Fragments arranged by size:
1154 1032 997 618 585 412 364 218
168 122 87 77 66 11 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 286 2161 3841 5916
Size: 286 1875 1680 2075
Fragments arranged by size:
2075 1875 1680 286
EcoICRI GAG'CTC
Cuts at: 0 1560 1910 3240 3501 3621 3831 5388 5916
Size: 1560 350 1330 261 120 210 1557 528
Fragments arranged by size:
1560 1557 1330 528 350 261 210 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 694 887 1501 1568 2107 2779 2812
Size: 560 134 193 614 67 539 672 33
Cuts at: 2812 2896 2968 3178 3199 3205 3349 3352 3367
Size: 84 72 210 21 6 144 3 15
Cuts at: 3367 3463 3556 3706 3790 3934 4042 4105 4258
Size: 96 93 150 84 144 108 63 153
Cuts at: 4258 4609 4630 4672 4876 4906 4983 5164 5221
Size: 351 21 42 204 30 77 181 57
Cuts at: 5221 5242 5254 5476 5551 5572 5677 5846 5916
Size: 21 12 222 75 21 105 169 70
Fragments arranged by size:
672 614 560 539 351 222 210 204
193 181 169 153 150 144 144 134
108 105 96 93 84 84 77 75
72 70 67 63 57 42 33 30
21 21 21 21 15 12 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1483 1855 2335 2674 2794 3370 3433 3730
Size: 1483 372 480 339 120 576 63 297
Cuts at: 3730 4477 5125 5916
Size: 747 648 791
Fragments arranged by size:
1483 791 747 648 576 480 372 339
297 120 63
EcoO109I rG'GnC_Cy
Cuts at: 0 111 3375 3447 3543 3723 4452 4470 4605
Size: 111 3264 72 96 180 729 18 135
Cuts at: 4605 4872 5082 5916
Size: 267 210 834
Fragments arranged by size:
3264 834 729 267 210 180 135 111
96 72 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 57 147 5577 5916
Size: 57 90 5430 339
Fragments arranged by size:
5430 339 90 57
HaeII r_GCGC'y
Cuts at: 0 1293 1925 4092 4714 5196 5455 5916
Size: 1293 632 2167 622 482 259 461
Fragments arranged by size:
2167 1293 632 622 482 461 259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 438 470 499 531 700 732 1015 1047
Size: 438 32 29 32 169 32 283 32
Cuts at: 1047 1093 1125 1172 1204 1376 1408 2041 2073
Size: 46 32 47 32 172 32 633 32
Cuts at: 2073 3367 3399 3547 3579 3778 3810 3916 3948
Size: 1294 32 148 32 199 32 106 32
Cuts at: 3948 4994 5026 5659 5691 5916
Size: 1046 32 633 32 225
Fragments arranged by size:
1294 1046 633 633 438 283 225 199
172 169 148 106 47 46 32 32
32 32 32 32 32 32 32 32
32 32 32 32 29
HincII GTy'rAC
Cuts at: 0 375 417 4701 5916
Size: 375 42 4284 1215
Fragments arranged by size:
4284 1215 375 42
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1856 4145 4355 5916
Size: 1856 2289 210 1561
Fragments arranged by size:
2289 1856 1561 210
MscI TGG'CCA
Cuts at: 0 1273 1894 2404 3256 3289 3919 5152 5320
Size: 1273 621 510 852 33 630 1233 168
Cuts at: 5320 5916
Size: 596
Fragments arranged by size:
1273 1233 852 630 621 596 510 168
33
MslI CAynn'nnrTG
Cuts at: 0 1315 2245 2449 2518 3880 4799 5916
Size: 1315 930 204 69 1362 919 1117
Fragments arranged by size:
1362 1315 1117 930 919 204 69
MspA1I CmG'CkG
Cuts at: 0 1129 1301 1419 1794 1965 1980 2094 2436
Size: 1129 172 118 375 171 15 114 342
Cuts at: 2436 2652 2685 2874 3252 4020 4053 4317 4641
Size: 216 33 189 378 768 33 264 324
Cuts at: 4641 4846 5095 5220 5655 5712 5916
Size: 205 249 125 435 57 204
Fragments arranged by size:
1129 768 435 378 375 342 324 264
249 216 205 204 189 172 171 125
118 114 57 33 33 15
NaeI GCC'GGC
Cuts at: 0 2134 5916
Size: 2134 3782
NcoI C'CATG_G
Cuts at: 0 995 5747 5916
Size: 995 4752 169
Fragments arranged by size:
4752 995 169
NdeI CA'TA_TG
Cuts at: 0 1284 5916
Size: 1284 4632
NgoMIV G'CCGG_C
Cuts at: 0 2132 5916
Size: 2132 3784
NotI GC'GGCC_GC
Cuts at: 0 4235 5916
Size: 4235 1681
NspI r_CATG'y
Cuts at: 0 423 495 1947 4707 5916
Size: 423 72 1452 2760 1209
Fragments arranged by size:
2760 1452 1209 423 72
PciI A'CATG_T
Cuts at: 0 419 5916
Size: 419 5497
PflMI CCAn_nnn'nTGG
Cuts at: 0 1152 4587 5409 5916
Size: 1152 3435 822 507
Fragments arranged by size:
3435 1152 822 507
PfoI T'CCnGG_A
Cuts at: 0 977 1370 1478 2162 3338 3368 3581 3950
Size: 977 393 108 684 1176 30 213 369
Cuts at: 3950 5180 5916
Size: 1230 736
Fragments arranged by size:
1230 1176 977 736 684 393 369 213
108 30
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 697 729 2990 3022 5916
Size: 697 32 2261 32 2894
Fragments arranged by size:
2894 2261 697 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 111 3375 3723 4872 5082 5916
Size: 111 3264 348 1149 210 834
Fragments arranged by size:
3264 1149 834 348 210 111
PspOMI G'GGCC_C
Cuts at: 0 3818 4469 4604 5916
Size: 3818 651 135 1312
Fragments arranged by size:
3818 1312 651 135
PspXI vC'TCGA_Gb
Cuts at: 0 4504 5368 5916
Size: 4504 864 548
Fragments arranged by size:
4504 864 548
PstI C_TGCA'G
Cuts at: 0 1064 1451 2537 2585 2690 3278 3794 3857
Size: 1064 387 1086 48 105 588 516 63
Cuts at: 3857 4142 4784 4851 4952 5570 5711 5916
Size: 285 642 67 101 618 141 205
Fragments arranged by size:
1086 1064 642 618 588 516 387 285
205 141 105 101 67 63 48
PvuII CAG'CTG
Cuts at: 0 1129 1419 1965 1980 2094 2436 2652 2685
Size: 1129 290 546 15 114 342 216 33
Cuts at: 2685 2874 3252 4020 4053 4641 4846 5220 5655
Size: 189 378 768 33 588 205 374 435
Cuts at: 5655 5916
Size: 261
Fragments arranged by size:
1129 768 588 546 435 378 374 342
290 261 216 205 189 114 33 33
15
SacI G_AGCT'C
Cuts at: 0 1562 1912 3242 3503 3623 3833 5390 5916
Size: 1562 350 1330 261 120 210 1557 526
Fragments arranged by size:
1562 1557 1330 526 350 261 210 120
SalI G'TCGA_C
Cuts at: 0 415 5916
Size: 415 5501
SanDI GG'GwC_CC
Cuts at: 0 111 5916
Size: 111 5805
SapI GCTCTTCn'nnn_
Cuts at: 0 1462 3136 4640 5916
Size: 1462 1674 1504 1276
Fragments arranged by size:
1674 1504 1462 1276
SexAI A'CCwGG_T
Cuts at: 0 4292 4544 5916
Size: 4292 252 1372
Fragments arranged by size:
4292 1372 252
SfcI C'TryA_G
Cuts at: 0 1060 1259 1447 1543 2533 2581 2674 2686
Size: 1060 199 188 96 990 48 93 12
Cuts at: 2686 2821 3274 3790 3853 4138 4780 4847 4948
Size: 135 453 516 63 285 642 67 101
Cuts at: 4948 5566 5707 5916
Size: 618 141 209
Fragments arranged by size:
1060 990 642 618 516 453 285 209
199 188 141 135 101 96 93 67
63 48 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 592 5916
Size: 592 5324
SmaI CCC'GGG
Cuts at: 0 3823 4747 5916
Size: 3823 924 1169
Fragments arranged by size:
3823 1169 924
SmlI C'TyrA_G
Cuts at: 0 1705 2479 2623 2911 3010 3241 3634 4504
Size: 1705 774 144 288 99 231 393 870
Cuts at: 4504 4714 5122 5368 5644 5770 5879 5916
Size: 210 408 246 276 126 109 37
Fragments arranged by size:
1705 870 774 408 393 288 276 246
231 210 144 126 109 99 37
SnaBI TAC'GTA
Cuts at: 0 347 5916
Size: 347 5569
SphI G_CATG'C
Cuts at: 0 495 5916
Size: 495 5421
SrfI GCCC'GGGC
Cuts at: 0 3823 5916
Size: 3823 2093
StuI AGG'CCT
Cuts at: 0 2236 4483 5916
Size: 2236 2247 1433
Fragments arranged by size:
2247 2236 1433
StyI C'CwwG_G
Cuts at: 0 254 872 995 1274 2567 2591 3653 3920
Size: 254 618 123 279 1293 24 1062 267
Cuts at: 3920 4448 4484 4556 5747 5903 5916
Size: 528 36 72 1191 156 13
Fragments arranged by size:
1293 1191 1062 618 528 279 267 254
156 123 72 36 24 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 258 1044 1587 2671 3973 4228 5916
Size: 258 786 543 1084 1302 255 1688
Fragments arranged by size:
1688 1302 1084 786 543 258 255
TatI w'GTAC_w
Cuts at: 0 422 1967 5600 5916
Size: 422 1545 3633 316
Fragments arranged by size:
3633 1545 422 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 1148 1180 1642 1674 5916
Size: 265 32 851 32 462 32 4242
Fragments arranged by size:
4242 851 462 265 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3561 3841 4546 5272 5916
Size: 3561 280 705 726 644
Fragments arranged by size:
3561 726 705 644 280
XbaI T'CTAG_A
Cuts at: 0 5815 5916
Size: 5815 101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1002 1041 1476 2217 3744 4080 4563 4605
Size: 1002 39 435 741 1527 336 483 42
Cuts at: 4605 4689 5451 5916
Size: 84 762 465
Fragments arranged by size:
1527 1002 762 741 483 465 435 336
84 42 39
XhoI C'TCGA_G
Cuts at: 0 4504 5122 5368 5879 5916
Size: 4504 618 246 511 37
Fragments arranged by size:
4504 618 511 246 37
XmaI C'CCGG_G
Cuts at: 0 3821 4745 5916
Size: 3821 924 1171
Fragments arranged by size:
3821 1171 924
XmnI GAAnn'nnTTC
Cuts at: 0 1408 2120 4186 5916
Size: 1408 712 2066 1730
Fragments arranged by size:
2066 1730 1408 712
ZraI GAC'GTC
Cuts at: 0 753 5916
Size: 753 5163
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
BaeI BamHI BanII BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NotI NspI PciI PflMI PfoI
PpiI PpuMI PspOMI PspXI PstI PvuII SacI SalI
SanDI SapI SexAI SfcI SfiI SmaI SmlI SnaBI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AscI AsiSI AvrII BanI
BbeI BfrBI BmtI BsaBI BsiWI BssHII BstEII BstZ17I
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NarI NheI NruI NsiI PacI PasI PmeI PmlI
PshAI PsiI PsrI PvuI RsrII SacII SbfI ScaI
SfoI SgfI SgrAI SpeI SspI SwaI