Restriction map for pF1KA0869
 (Linear) (Six-base) MAPSORT of: KIAA0869.seq  Check: 7285  from: 1  to: 3339



KIAA0869.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1233    3339 
   Size:       1233    2106
 
AatII G_ACGT'C

Cuts at:      0    3338    3339 
   Size:       3338       1
 
AccI GT'mk_AC

Cuts at:      0      65    2329    3339 
   Size:         65    2264    1010

  Fragments arranged by size: 

                2264    1010      65
 
Acc65I G'GTAC_C

Cuts at:      0    2642    3339 
   Size:       2642     697
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     541     604     973    1072    1358    1913    2017 
   Size:        307     234      63     369      99     286     555     104

Cuts at:   2017    3055    3166    3339 
   Size:       1038     111     173

  Fragments arranged by size: 

                1038     555     369     307     286     234     173     111
                 104      99      63
 
AflII C'TTAA_G

Cuts at:      0    2229    3339 
   Size:       2229    1110
 
AflIII A'CryG_T

Cuts at:      0    2813    3339 
   Size:       2813     526
 
AhdI GACnn_n'nnGTC

Cuts at:      0     759     777    2439    2899    3339 
   Size:        759      18    1662     460     440

  Fragments arranged by size: 

                1662     759     460     440      18
 
AleI CACnn'nnGTG

Cuts at:      0    2170    2704    3173    3339 
   Size:       2170     534     469     166

  Fragments arranged by size: 

                2170     534     469     166
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     410    3339 
   Size:        410    2929
 
AlwNI CAG_nnn'CTG

Cuts at:      0     360     390     582     831    1296    1560    1882    1920 
   Size:        360      30     192     249     465     264     322      38

Cuts at:   1920    1936    2422    2511    3339 
   Size:         16     486      89     828

  Fragments arranged by size: 

                 828     486     465     360     322     264     249     192
                  89      38      30      16
 
ApaLI G'TGCA_C

Cuts at:      0     323    3220    3339 
   Size:        323    2897     119

  Fragments arranged by size: 

                2897     323     119
 
ApoI r'AATT_y

Cuts at:      0    1982    3339 
   Size:       1982    1357
 
AseI AT'TA_AT

Cuts at:      0    2032    3339 
   Size:       2032    1307
 
AvaI C'yCGr_G

Cuts at:      0     251     506     858    1151    1482    3339 
   Size:        251     255     352     293     331    1857

  Fragments arranged by size: 

                1857     352     331     293     255     251
 
BamHI G'GATC_C

Cuts at:      0     246    1318    3339 
   Size:        246    1072    2021

  Fragments arranged by size: 

                2021    1072     246
 
BanI G'GyrC_C

Cuts at:      0    1576    2284    2642    2731    3339 
   Size:       1576     708     358      89     608

  Fragments arranged by size: 

                1576     708     608     358      89
 
BanII G_rGCy'C

Cuts at:      0     421     844    1342    2387    3044    3339 
   Size:        421     423     498    1045     657     295

  Fragments arranged by size: 

                1045     657     498     423     421     295
 
BbeI G_GCGC'C

Cuts at:      0    2288    3339 
   Size:       2288    1051
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1709    2230    2522    3339 
   Size:       1709     521     292     817

  Fragments arranged by size: 

                1709     817     521     292
 
BbvCI CC'TCA_GC

Cuts at:      0    3130    3339 
   Size:       3130     209
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2205    3339 
   Size:       2205    1134
 
BglI GCCn_nnn'nGGC

Cuts at:      0     313    2282    3339 
   Size:        313    1969    1057

  Fragments arranged by size: 

                1969    1057     313
 
BglII A'GATC_T

Cuts at:      0     335     708    3023    3339 
   Size:        335     373    2315     316

  Fragments arranged by size: 

                2315     373     335     316
 
Bme1580I G_kGCm'C

Cuts at:      0     327    2734    3224    3339 
   Size:        327    2407     490     115

  Fragments arranged by size: 

                2407     490     327     115
 
BmgBI CAC'GTC

Cuts at:      0    1328    3339 
   Size:       1328    2011
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1930    3339 
   Size:       1930    1409
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1739    2348    2640    3339 
   Size:       1739     609     292     699

  Fragments arranged by size: 

                1739     699     609     292
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     526     817    1129    1246    1477    1792    1904 
   Size:        382     144     291     312     117     231     315     112

Cuts at:   1904    2494    2544    2908    2992    3160    3339 
   Size:        590      50     364      84     168     179

  Fragments arranged by size: 

                 590     382     364     315     312     291     231     179
                 168     144     117     112      84      50
 
Bpu10I CC'TnA_GC

Cuts at:      0     614     641    3130    3339 
   Size:        614      27    2489     209

  Fragments arranged by size: 

                2489     614     209      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     672    1263    2371    3028    3339 
   Size:        672     591    1108     657     311

  Fragments arranged by size: 

                1108     672     657     591     311
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     441     767    2530    2662    2674    3339 
   Size:        441     326    1763     132      12     665

  Fragments arranged by size: 

                1763     665     441     326     132      12
 
BsaAI yAC'GTr

Cuts at:      0    2814    3339 
   Size:       2814     525
 
BsaBI GATnn'nnATC

Cuts at:      0    1027    3310    3339 
   Size:       1027    2283      29

  Fragments arranged by size: 

                2283    1027      29
 
BsaHI Gr'CG_yC

Cuts at:      0    2156    2285    3335    3339 
   Size:       2156     129    1050       4

  Fragments arranged by size: 

                2156    1050     129       4
 
BsaWI w'CCGG_w

Cuts at:      0    2138    2720    2903    3339 
   Size:       2138     582     183     436

  Fragments arranged by size: 

                2138     582     436     183
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2823    2853    3339 
   Size:       2823      30     486

  Fragments arranged by size: 

                2823     486      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52    1057    1356    1611    2548    2584    2587    2590 
   Size:         52    1005     299     255     937      36       3       3

Cuts at:   2590    2599    2602    2605    3339 
   Size:          9       3       3     734

  Fragments arranged by size: 

                1005     937     734     299     255      52      36       9
                   3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0      32     414    1262    1800    2646    3315    3339 
   Size:         32     382     848     538     846     669      24

  Fragments arranged by size: 

                 848     846     669     538     382      32      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1240    1933    3339 
   Size:       1240     693    1406

  Fragments arranged by size: 

                1406    1240     693
 
BsiEI CG_ry'CG

Cuts at:      0      10    3339 
   Size:         10    3329
 
BsiHKAI G_wGCw'C

Cuts at:      0     327     844    2387    2767    3044    3170    3224    3339 
   Size:        327     517    1543     380     277     126      54     115

  Fragments arranged by size: 

                1543     517     380     327     277     126     115      54
            
 
Bsp1286I G_dGCh'C

Cuts at:      0     327     421     844    1342    2387    2734    2767    3044 
   Size:        327      94     423     498    1045     347      33     277

Cuts at:   3044    3170    3224    3339 
   Size:        126      54     115

  Fragments arranged by size: 

                1045     498     423     347     327     277     126     115
                  94      54      33
 
BspEI T'CCGG_A

Cuts at:      0    2138    2903    3339 
   Size:       2138     765     436

  Fragments arranged by size: 

                2138     765     436
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1148    1233    2137    2990    3339 
   Size:       1148      85     904     853     349

  Fragments arranged by size: 

                1148     904     853     349      85
 
BsrBI CCG'CTC

Cuts at:      0     132    2777    3163    3339 
   Size:        132    2645     386     176

  Fragments arranged by size: 

                2645     386     176     132
 
BsrFI r'CCGG_y

Cuts at:      0    1016    1468    1766    2312    2819    3339 
   Size:       1016     452     298     546     507     520

  Fragments arranged by size: 

                1016     546     520     507     452     298
 
BssHII G'CGCG_C

Cuts at:      0      43    3339 
   Size:         43    3296
 
BssSI C'ACGA_G

Cuts at:      0     547    1504    3339 
   Size:        547     957    1835

  Fragments arranged by size: 

                1835     957     547
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     552    1560    1972    2129    3339 
   Size:        552    1008     412     157    1210

  Fragments arranged by size: 

                1210    1008     552     412     157
 
BstBI TT'CG_AA

Cuts at:      0    2877    3339 
   Size:       2877     462
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1831    3339 
   Size:       1831    1508
 
BstYI r'GATC_y

Cuts at:      0     246     335     473     708     956    1318    3023    3339 
   Size:        246      89     138     235     248     362    1705     316

  Fragments arranged by size: 

                1705     362     316     248     246     235     138      89
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1302    2415    2714    3339 
   Size:       1302    1113     299     625

  Fragments arranged by size: 

                1302    1113     625     299
 
BtgI C'CryG_G

Cuts at:      0     109    3072    3339 
   Size:        109    2963     267

  Fragments arranged by size: 

                2963     267     109
 
BtsI GCAGTG_nn'

Cuts at:      0     494    1973    2471    3339 
   Size:        494    1479     498     868

  Fragments arranged by size: 

                1479     868     498     494
 
ClaI AT'CG_AT

Cuts at:      0     593    3339 
   Size:        593    2746
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     222     257     549     584    3080    3115    3339 
   Size:        222      35     292      35    2496      35     224

  Fragments arranged by size: 

                2496     292     224     222      35      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      61    3339 
   Size:         61    3278
 
EaeI y'GGCC_r

Cuts at:      0       7     965    1517    1545    3339 
   Size:          7     958     552      28    1794

  Fragments arranged by size: 

                1794     958     552      28       7
 
EagI C'GGCC_G

Cuts at:      0       7    3339 
   Size:          7    3332
 
EarI CTCTTCn'nnn_

Cuts at:      0     173     517     920    2264    2473    2561    2594    3339 
   Size:        173     344     403    1344     209      88      33     745

  Fragments arranged by size: 

                1344     745     403     344     209     173      88      33
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2317    3109    3339 
   Size:       2317     792     230

  Fragments arranged by size: 

                2317     792     230
 
EcoICRI GAG'CTC

Cuts at:      0     842    2385    3042    3339 
   Size:        842    1543     657     297

  Fragments arranged by size: 

                1543     842     657     297
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     382     526     541     604     817     973    1072 
   Size:        307      75     144      15      63     213     156      99

Cuts at:   1072    1129    1246    1358    1477    1792    1904    1913    2017 
   Size:         57     117     112     119     315     112       9     104

Cuts at:   2017    2494    2544    2908    2992    3055    3160    3166    3339 
   Size:        477      50     364      84      63     105       6     173

  Fragments arranged by size: 

                 477     364     315     307     213     173     156     144
                 119     117     112     112     105     104      99      84
                  75      63      63      57      50      15       9       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      25    2302    2414    3339 
   Size:         25    2277     112     925

  Fragments arranged by size: 

                2277     925     112      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1137    2419    2797    3339 
   Size:       1137    1282     378     542

  Fragments arranged by size: 

                1282    1137     542     378
 
EcoRI G'AATT_C

Cuts at:      0    1982    3339 
   Size:       1982    1357
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     759    3339 
   Size:        759    2580
 
HaeII r_GCGC'y

Cuts at:      0     360    1174    1754    2288    3339 
   Size:        360     814     580     534    1051

  Fragments arranged by size: 

                1051     814     580     534     360
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     231     263     745     763     777     795     826     858 
   Size:        231      32     482      18      14      18      31      32

Cuts at:    858    2133    2165    2225    2257    2425    2457    2885    2917 
   Size:       1275      32      60      32     168      32     428      32

Cuts at:   2917    3339 
   Size:        422

  Fragments arranged by size: 

                1275     482     428     422     231     168      60      32
                  32      32      32      32      32      31      18      18
                  14
 
HincII GTy'rAC

Cuts at:      0     513    2330    3339 
   Size:        513    1817    1009

  Fragments arranged by size: 

                1817    1009     513
 
HindIII A'AGCT_T

Cuts at:      0     864     970    3339 
   Size:        864     106    2369

  Fragments arranged by size: 

                2369     864     106
 
KasI G'GCGC_C

Cuts at:      0    2284    3339 
   Size:       2284    1055
 
KpnI G_GTAC'C

Cuts at:      0    2646    3339 
   Size:       2646     693
 
MfeI C'AATT_G

Cuts at:      0    3010    3339 
   Size:       3010     329
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     180    2432    3339 
   Size:        180    2252     907

  Fragments arranged by size: 

                2252     907     180
 
MscI TGG'CCA

Cuts at:      0     967    1519    3339 
   Size:        967     552    1820

  Fragments arranged by size: 

                1820     967     552
 
MslI CAynn'nnrTG

Cuts at:      0    1508    2170    2704    3173    3339 
   Size:       1508     662     534     469     166

  Fragments arranged by size: 

                1508     662     534     469     166
 
MspA1I CmG'CkG

Cuts at:      0      98     111     963    1107    1266    1416    1800    2043 
   Size:         98      13     852     144     159     150     384     243

Cuts at:   2043    2522    3054    3208    3339 
   Size:        479     532     154     131

  Fragments arranged by size: 

                 852     532     479     384     243     159     154     150
                 144     131      98      13
 
NaeI GCC'GGC

Cuts at:      0    2821    3339 
   Size:       2821     518
 
NarI GG'CG_CC

Cuts at:      0    2285    3339 
   Size:       2285    1054
 
NdeI CA'TA_TG

Cuts at:      0     186     281    3339 
   Size:        186      95    3058

  Fragments arranged by size: 

                3058     186      95
 
NgoMIV G'CCGG_C

Cuts at:      0    2819    3339 
   Size:       2819     520
 
NspI r_CATG'y

Cuts at:      0     397    1290    3339 
   Size:        397     893    2049

  Fragments arranged by size: 

                2049     893     397
 
PasI CC'CwG_GG

Cuts at:      0    1089    1824    2742    3339 
   Size:       1089     735     918     597

  Fragments arranged by size: 

                1089     918     735     597
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      98     199    3339 
   Size:         98     101    3140

  Fragments arranged by size: 

                3140     101      98
 
PfoI T'CCnGG_A

Cuts at:      0    1454    3339 
   Size:       1454    1885
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     518     541     550     573    3339 
   Size:        518      23       9      23    2766

  Fragments arranged by size: 

                2766     518      23      23       9
 
PpuMI rG'GwC_Cy

Cuts at:      0    1137    2797    3339 
   Size:       1137    1660     542

  Fragments arranged by size: 

                1660    1137     542
 
PspXI vC'TCGA_Gb

Cuts at:      0     251    3339 
   Size:        251    3088
 
PstI C_TGCA'G

Cuts at:      0     557    1145    1433    1556    2987    3330    3339 
   Size:        557     588     288     123    1431     343       9

  Fragments arranged by size: 

                1431     588     557     343     288     123       9
 
PvuII CAG'CTG

Cuts at:      0      98     963    1107    1266    1416    1800    2043    2522 
   Size:         98     865     144     159     150     384     243     479

Cuts at:   2522    3054    3208    3339 
   Size:        532     154     131

  Fragments arranged by size: 

                 865     532     479     384     243     159     154     150
                 144     131      98
 
SacI G_AGCT'C

Cuts at:      0     844    2387    3044    3339 
   Size:        844    1543     657     295

  Fragments arranged by size: 

                1543     844     657     295
 
SacII CC_GC'GG

Cuts at:      0     112    3339 
   Size:        112    3227
 
SalI G'TCGA_C

Cuts at:      0    2328    3339 
   Size:       2328    1011
 
SbfI CC_TGCA'GG

Cuts at:      0    1145    1556    2987    3339 
   Size:       1145     411    1431     352

  Fragments arranged by size: 

                1431    1145     411     352
 
SexAI A'CCwGG_T

Cuts at:      0     296     341    3339 
   Size:        296      45    2998

  Fragments arranged by size: 

                2998     296      45
 
SfcI C'TryA_G

Cuts at:      0     553    1141    1429    1552    2983    3326    3339 
   Size:        553     588     288     123    1431     343      13

  Fragments arranged by size: 

                1431     588     553     343     288     123      13
 
SfoI GGC'GCC

Cuts at:      0    2286    3339 
   Size:       2286    1053
 
SmaI CCC'GGG

Cuts at:      0     508    1153    3339 
   Size:        508     645    2186

  Fragments arranged by size: 

                2186     645     508
 
SmlI C'TyrA_G

Cuts at:      0     251     651    1278    2229    2386    3043    3339 
   Size:        251     400     627     951     157     657     296

  Fragments arranged by size: 

                 951     657     627     400     296     251     157
 
SphI G_CATG'C

Cuts at:      0     397    1290    3339 
   Size:        397     893    2049

  Fragments arranged by size: 

                2049     893     397
 
SspI AAT'ATT

Cuts at:      0    2030    3339 
   Size:       2030    1309
 
StuI AGG'CCT

Cuts at:      0     985    3339 
   Size:        985    2354
 
StyI C'CwwG_G

Cuts at:      0    1691    1740    3212    3339 
   Size:       1691      49    1472     127

  Fragments arranged by size: 

                1691    1472     127      49
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1174    1912    1960    2913    3173    3339 
   Size:       1174     738      48     953     260     166

  Fragments arranged by size: 

                1174     953     738     260     166      48
 
TatI w'GTAC_w

Cuts at:      0     255    3339 
   Size:        255    3084
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2823    2855    3339 
   Size:       2823      32     484

  Fragments arranged by size: 

                2823     484      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1242    3339 
   Size:       1242    2097
 
XhoI C'TCGA_G

Cuts at:      0     251    3339 
   Size:        251    3088
 
XmaI C'CCGG_G

Cuts at:      0     506    1151    3339 
   Size:        506     645    2188

  Fragments arranged by size: 

                2188     645     506
 
ZraI GAC'GTC

Cuts at:      0    3336    3339 
   Size:       3336       3
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI    AflII   AflIII     AhdI
     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI     BtsI
     ClaI    CspCI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NspI     PasI    PflMI     PfoI
     PpiI    PpuMI    PspXI     PstI    PvuII     SacI    SacII     SalI
     SbfI    SexAI     SfcI     SfoI     SmaI     SmlI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmaI
     ZraI

 Enzymes that do not cut: 

     AclI     AfeI     AgeI     AloI     ApaI     AscI    AsiSI    AvrII
     BaeI     BcgI     BclI    BfrBI     BlpI     BmtI    BsiWI     BsmI
    BsmBI    BspHI    BsrDI    BsrGI   BstEII  BstZ17I    BtgZI     DraI
   DraIII    EcoRV     FseI     FspI    FspAI     HpaI     MluI     NcoI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
    PshAI     PsiI   PspOMI     PsrI     PvuI    RsrII    SanDI     SapI
     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SwaI
  Tth111I     XbaI     XmnI
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