Restriction map for
pF1KSDA0871
(Linear) (Six-base) MAPSORT of: KIAA0871.seq Check: 1300 from: 1 to: 1407
KIAA0871.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 826 1407
Size: 773 53 581
Fragments arranged by size:
773 581 53
AflII C'TTAA_G
Cuts at: 0 466 1407
Size: 466 941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1238 1407
Size: 1238 169
AlwNI CAG_nnn'CTG
Cuts at: 0 1191 1407
Size: 1191 216
ApoI r'AATT_y
Cuts at: 0 88 574 1407
Size: 88 486 833
Fragments arranged by size:
833 486 88
AseI AT'TA_AT
Cuts at: 0 511 1407
Size: 511 896
BanII G_rGCy'C
Cuts at: 0 1209 1407
Size: 1209 198
BbsI GAAGACnn'nnnn_
Cuts at: 0 686 1300 1407
Size: 686 614 107
Fragments arranged by size:
686 614 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 9 43 571 605 1407
Size: 9 34 528 34 802
Fragments arranged by size:
802 528 34 34 9
BclI T'GATC_A
Cuts at: 0 548 1407
Size: 548 859
BfrBI ATG'CAT
Cuts at: 0 859 1407
Size: 859 548
BglII A'GATC_T
Cuts at: 0 453 1407
Size: 453 954
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 133 1407
Size: 133 1274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1108 1159 1407
Size: 286 822 51 248
Fragments arranged by size:
822 286 248 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1195 1407
Size: 709 486 212
Fragments arranged by size:
709 486 212
BsaI GGTCTCn'nnnn_
Cuts at: 0 64 1407
Size: 64 1343
BsaBI GATnn'nnATC
Cuts at: 0 1096 1407
Size: 1096 311
BsaHI Gr'CG_yC
Cuts at: 0 13 1407
Size: 13 1394
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 904 1407
Size: 904 503
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 1407
Size: 1397 10
BsiEI CG_ry'CG
Cuts at: 0 23 1407
Size: 23 1384
BsiHKAI G_wGCw'C
Cuts at: 0 329 1209 1407
Size: 329 880 198
Fragments arranged by size:
880 329 198
BsmI GAATG_Cn'
Cuts at: 0 828 1407
Size: 828 579
BsmBI CGTCTCn'nnnn_
Cuts at: 0 752 1407
Size: 752 655
Bsp1286I G_dGCh'C
Cuts at: 0 329 1209 1407
Size: 329 880 198
Fragments arranged by size:
880 329 198
BspHI T'CATG_A
Cuts at: 0 215 596 1407
Size: 215 381 811
Fragments arranged by size:
811 381 215
BstYI r'GATC_y
Cuts at: 0 177 453 1407
Size: 177 276 954
Fragments arranged by size:
954 276 177
BtgI C'CryG_G
Cuts at: 0 65 101 1407
Size: 65 36 1306
Fragments arranged by size:
1306 65 36
DraI TTT'AAA
Cuts at: 0 1046 1407
Size: 1046 361
DraIII CAC_nnn'GTG
Cuts at: 0 1027 1407
Size: 1027 380
EaeI y'GGCC_r
Cuts at: 0 224 1407
Size: 224 1183
EarI CTCTTCn'nnn_
Cuts at: 0 483 1046 1184 1283 1407
Size: 483 563 138 99 124
Fragments arranged by size:
563 483 138 124 99
EcoICRI GAG'CTC
Cuts at: 0 1207 1407
Size: 1207 200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 773 826 1108 1159 1407
Size: 286 487 53 282 51 248
Fragments arranged by size:
487 286 282 248 53 51
EcoO109I rG'GnC_Cy
Cuts at: 0 391 988 1407
Size: 391 597 419
Fragments arranged by size:
597 419 391
EcoRI G'AATT_C
Cuts at: 0 574 1407
Size: 574 833
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 673 705 1407
Size: 127 32 514 32 702
Fragments arranged by size:
702 514 127 32 32
HindIII A'AGCT_T
Cuts at: 0 412 542 1407
Size: 412 130 865
Fragments arranged by size:
865 412 130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 678 896 1407
Size: 43 635 218 511
Fragments arranged by size:
635 511 218 43
MscI TGG'CCA
Cuts at: 0 226 1407
Size: 226 1181
MslI CAynn'nnrTG
Cuts at: 0 220 821 1407
Size: 220 601 586
Fragments arranged by size:
601 586 220
MspA1I CmG'CkG
Cuts at: 0 1191 1407
Size: 1191 216
NcoI C'CATG_G
Cuts at: 0 65 101 1407
Size: 65 36 1306
Fragments arranged by size:
1306 65 36
NsiI A_TGCA'T
Cuts at: 0 861 1407
Size: 861 546
PfoI T'CCnGG_A
Cuts at: 0 459 1407
Size: 459 948
PpuMI rG'GwC_Cy
Cuts at: 0 988 1407
Size: 988 419
PstI C_TGCA'G
Cuts at: 0 1011 1238 1407
Size: 1011 227 169
Fragments arranged by size:
1011 227 169
PvuII CAG'CTG
Cuts at: 0 1191 1407
Size: 1191 216
SacI G_AGCT'C
Cuts at: 0 1209 1407
Size: 1209 198
SanDI GG'GwC_CC
Cuts at: 0 988 1407
Size: 988 419
SapI GCTCTTCn'nnn_
Cuts at: 0 483 1407
Size: 483 924
SfcI C'TryA_G
Cuts at: 0 1007 1234 1407
Size: 1007 227 173
Fragments arranged by size:
1007 227 173
SmlI C'TyrA_G
Cuts at: 0 466 724 1210 1407
Size: 466 258 486 197
Fragments arranged by size:
486 466 258 197
StyI C'CwwG_G
Cuts at: 0 65 101 1407
Size: 65 36 1306
Fragments arranged by size:
1306 65 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 1407
Size: 37 1370
XbaI T'CTAG_A
Cuts at: 0 396 1407
Size: 396 1011
Enzymes that do cut:
AcuI AflII AlfI AlwNI ApoI AseI BanII BbsI
BcgI BclI BfrBI BglII BplI BpmI BpuEI BsaI
BsaBI BsaHI BseRI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BstYI BtgI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI Hin4I HindIII MmeI MscI
MslI MspA1I NcoI NsiI PfoI PpuMI PstI PvuII
SacI SanDI SapI SfcI SmlI StyI TaqII XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BciVI BglI
BlpI Bme1580I BmgBI BmrI BmtI Bpu10I BsaAI BsaWI
BsaXI BseYI BsiWI BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DrdI EagI EciI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI