Restriction map for
pF1KA0877
(Linear) (Six-base) MAPSORT of: KIAA0877.seq Check: 958 from: 1 to: 1656
KIAA0877.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 796 1394 1656
Size: 796 598 262
Fragments arranged by size:
796 598 262
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1656
Size: 280 1376
AflIII A'CryG_T
Cuts at: 0 948 1064 1656
Size: 948 116 592
Fragments arranged by size:
948 592 116
AleI CACnn'nnGTG
Cuts at: 0 1065 1067 1656
Size: 1065 2 589
Fragments arranged by size:
1065 589 2
ApoI r'AATT_y
Cuts at: 0 710 760 1523 1656
Size: 710 50 763 133
Fragments arranged by size:
763 710 133 50
BanI G'GyrC_C
Cuts at: 0 1291 1656
Size: 1291 365
BbsI GAAGACnn'nnnn_
Cuts at: 0 1367 1656
Size: 1367 289
BclI T'GATC_A
Cuts at: 0 969 1656
Size: 969 687
BfrBI ATG'CAT
Cuts at: 0 994 1656
Size: 994 662
BmrI ACTGGGnnnn_n'
Cuts at: 0 1191 1656
Size: 1191 465
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 73 1656
Size: 73 1583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1077 1392 1656
Size: 1077 315 264
Fragments arranged by size:
1077 315 264
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 182 212 245 275 968 998 1656
Size: 182 30 33 30 693 30 658
Fragments arranged by size:
693 658 182 33 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 62 1656
Size: 62 1594
BseYI C'CCAG_C
Cuts at: 0 1552 1656
Size: 1552 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 1310 1656
Size: 824 486 346
Fragments arranged by size:
824 486 346
BsrDI GCAATG_nn'
Cuts at: 0 608 994 1169 1656
Size: 608 386 175 487
Fragments arranged by size:
608 487 386 175
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1285 1656
Size: 1285 371
BstEII G'GTnAC_C
Cuts at: 0 39 1656
Size: 39 1617
BstYI r'GATC_y
Cuts at: 0 90 1495 1539 1656
Size: 90 1405 44 117
Fragments arranged by size:
1405 117 90 44
BstZ17I GTA'TAC
Cuts at: 0 797 1395 1656
Size: 797 598 261
Fragments arranged by size:
797 598 261
BtsI GCAGTG_nn'
Cuts at: 0 1286 1656
Size: 1286 370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1180 1215 1656
Size: 1180 35 441
Fragments arranged by size:
1180 441 35
DraI TTT'AAA
Cuts at: 0 128 1656
Size: 128 1528
EarI CTCTTCn'nnn_
Cuts at: 0 1054 1469 1656
Size: 1054 415 187
Fragments arranged by size:
1054 415 187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1656
Size: 280 1376
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1258 1656
Size: 1258 398
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 243 275 1031 1063 1656
Size: 243 32 756 32 593
Fragments arranged by size:
756 593 243 32 32
HindIII A'AGCT_T
Cuts at: 0 270 348 1656
Size: 270 78 1308
Fragments arranged by size:
1308 270 78
MfeI C'AATT_G
Cuts at: 0 1000 1656
Size: 1000 656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 1390 1656
Size: 13 1377 266
Fragments arranged by size:
1377 266 13
MslI CAynn'nnrTG
Cuts at: 0 1065 1067 1133 1656
Size: 1065 2 66 523
Fragments arranged by size:
1065 523 66 2
MspA1I CmG'CkG
Cuts at: 0 808 1656
Size: 808 848
NsiI A_TGCA'T
Cuts at: 0 996 1656
Size: 996 660
NspI r_CATG'y
Cuts at: 0 952 1068 1656
Size: 952 116 588
Fragments arranged by size:
952 588 116
PciI A'CATG_T
Cuts at: 0 948 1064 1656
Size: 948 116 592
Fragments arranged by size:
948 592 116
PsiI TTA'TAA
Cuts at: 0 773 1656
Size: 773 883
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1612 1644 1656
Size: 1612 32 12
Fragments arranged by size:
1612 32 12
ScaI AGT'ACT
Cuts at: 0 1266 1656
Size: 1266 390
SmlI C'TyrA_G
Cuts at: 0 1092 1371 1656
Size: 1092 279 285
Fragments arranged by size:
1092 285 279
SspI AAT'ATT
Cuts at: 0 1206 1656
Size: 1206 450
StuI AGG'CCT
Cuts at: 0 194 1656
Size: 194 1462
StyI C'CwwG_G
Cuts at: 0 391 1020 1656
Size: 391 629 636
Fragments arranged by size:
636 629 391
TatI w'GTAC_w
Cuts at: 0 411 1125 1264 1656
Size: 411 714 139 392
Fragments arranged by size:
714 411 392 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 245 277 1656
Size: 245 32 1379
Fragments arranged by size:
1379 245 32
Tth111I GACn'n_nGTC
Cuts at: 0 59 1656
Size: 59 1597
XbaI T'CTAG_A
Cuts at: 0 1470 1656
Size: 1470 186
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 970 1302 1656
Size: 970 332 354
Fragments arranged by size:
970 354 332
Enzymes that do cut:
AccI AcuI AflIII AleI ApoI BanI BbsI BclI
BfrBI BmrI BplI BpuEI BsaXI BseRI BseYI BsgI
BsrDI BstAPI BstEII BstYI BstZ17I BtsI CspCI DraI
EarI Eco57MI FalI Hin4I HindIII MfeI MmeI MslI
MspA1I NsiI NspI PciI PsiI PsrI ScaI SmlI
SspI StuI StyI TatI TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmtI BpmI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstXI Bsu36I BtgI
BtgZI ClaI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TaqII
XhoI XmaI XmnI ZraI