Restriction map for
pF1KA0878
(Linear) (Six-base) MAPSORT of: KIAA0878.seq Check: 5439 from: 1 to: 1833
KIAA0878.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
Acc65I G'GTAC_C
Cuts at: 0 400 1833
Size: 400 1433
AclI AA'CG_TT
Cuts at: 0 302 1833
Size: 302 1531
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 725 947 1671 1833
Size: 497 228 222 724 162
Fragments arranged by size:
724 497 228 222 162
AleI CACnn'nnGTG
Cuts at: 0 1526 1833
Size: 1526 307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 142 1833
Size: 142 1691
ApoI r'AATT_y
Cuts at: 0 369 1274 1702 1833
Size: 369 905 428 131
Fragments arranged by size:
905 428 369 131
AseI AT'TA_AT
Cuts at: 0 1163 1833
Size: 1163 670
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1274 1307 1833
Size: 1274 33 526
Fragments arranged by size:
1274 526 33
BamHI G'GATC_C
Cuts at: 0 482 1833
Size: 482 1351
BanI G'GyrC_C
Cuts at: 0 400 1833
Size: 400 1433
BbsI GAAGACnn'nnnn_
Cuts at: 0 610 1260 1833
Size: 610 650 573
Fragments arranged by size:
650 610 573
BclI T'GATC_A
Cuts at: 0 290 1833
Size: 290 1543
BfrBI ATG'CAT
Cuts at: 0 1139 1833
Size: 1139 694
BglI GCCn_nnn'nGGC
Cuts at: 0 66 1303 1457 1833
Size: 66 1237 154 376
Fragments arranged by size:
1237 376 154 66
BmgBI CAC'GTC
Cuts at: 0 765 1833
Size: 765 1068
BmtI G_CTAG'C
Cuts at: 0 953 1833
Size: 953 880
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 113 1833
Size: 113 1720
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1213 1833
Size: 1213 620
BsaI GGTCTCn'nnnn_
Cuts at: 0 108 1833
Size: 108 1725
BsaHI Gr'CG_yC
Cuts at: 0 998 1833
Size: 998 835
BsaWI w'CCGG_w
Cuts at: 0 156 1833
Size: 156 1677
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1426 1456 1833
Size: 1426 30 377
Fragments arranged by size:
1426 377 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 578 1833
Size: 578 1255
BseYI C'CCAG_C
Cuts at: 0 21 1452 1547 1833
Size: 21 1431 95 286
Fragments arranged by size:
1431 286 95 21
BsiEI CG_ry'CG
Cuts at: 0 61 149 1833
Size: 61 88 1684
Fragments arranged by size:
1684 88 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1613 1833
Size: 1613 220
BsrDI GCAATG_nn'
Cuts at: 0 1664 1833
Size: 1664 169
BsrFI r'CCGG_y
Cuts at: 0 56 1833
Size: 56 1777
BsrGI T'GTAC_A
Cuts at: 0 427 1433 1833
Size: 427 1006 400
Fragments arranged by size:
1006 427 400
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1560 1833
Size: 1560 273
BstBI TT'CG_AA
Cuts at: 0 1271 1833
Size: 1271 562
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1314 1833
Size: 1314 519
BstYI r'GATC_y
Cuts at: 0 482 909 1710 1833
Size: 482 427 801 123
Fragments arranged by size:
801 482 427 123
Bsu36I CC'TnA_GG
Cuts at: 0 1171 1833
Size: 1171 662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1618 1653 1833
Size: 1618 35 180
Fragments arranged by size:
1618 180 35
DraI TTT'AAA
Cuts at: 0 788 1145 1833
Size: 788 357 688
Fragments arranged by size:
788 688 357
DraIII CAC_nnn'GTG
Cuts at: 0 15 1833
Size: 15 1818
DrdI GACnn_nn'nnGTC
Cuts at: 0 220 1413 1833
Size: 220 1193 420
Fragments arranged by size:
1193 420 220
EaeI y'GGCC_r
Cuts at: 0 58 137 1753 1833
Size: 58 79 1616 80
Fragments arranged by size:
1616 80 79 58
EagI C'GGCC_G
Cuts at: 0 58 1833
Size: 58 1775
EarI CTCTTCn'nnn_
Cuts at: 0 410 856 1009 1833
Size: 410 446 153 824
Fragments arranged by size:
824 446 410 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 725 947 1671 1833
Size: 497 228 222 724 162
Fragments arranged by size:
724 497 228 222 162
EcoRV GAT'ATC
Cuts at: 0 1080 1833
Size: 1080 753
HaeII r_GCGC'y
Cuts at: 0 22 278 1833
Size: 22 256 1555
Fragments arranged by size:
1555 256 22
HincII GTy'rAC
Cuts at: 0 1602 1833
Size: 1602 231
KpnI G_GTAC'C
Cuts at: 0 404 1833
Size: 404 1429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 551 1833
Size: 510 41 1282
Fragments arranged by size:
1282 510 41
MslI CAynn'nnrTG
Cuts at: 0 286 1312 1526 1833
Size: 286 1026 214 307
Fragments arranged by size:
1026 307 286 214
MspA1I CmG'CkG
Cuts at: 0 1456 1551 1833
Size: 1456 95 282
Fragments arranged by size:
1456 282 95
NheI G'CTAG_C
Cuts at: 0 949 1833
Size: 949 884
NsiI A_TGCA'T
Cuts at: 0 1141 1833
Size: 1141 692
NspI r_CATG'y
Cuts at: 0 434 1563 1833
Size: 434 1129 270
Fragments arranged by size:
1129 434 270
PflMI CCAn_nnn'nTGG
Cuts at: 0 15 759 1833
Size: 15 744 1074
Fragments arranged by size:
1074 744 15
PfoI T'CCnGG_A
Cuts at: 0 1807 1833
Size: 1807 26
PsiI TTA'TAA
Cuts at: 0 719 776 1217 1833
Size: 719 57 441 616
Fragments arranged by size:
719 616 441 57
PstI C_TGCA'G
Cuts at: 0 1520 1556 1833
Size: 1520 36 277
Fragments arranged by size:
1520 277 36
PvuII CAG'CTG
Cuts at: 0 1456 1551 1833
Size: 1456 95 282
Fragments arranged by size:
1456 282 95
SfcI C'TryA_G
Cuts at: 0 1516 1552 1833
Size: 1516 36 281
Fragments arranged by size:
1516 281 36
SmlI C'TyrA_G
Cuts at: 0 1228 1833
Size: 1228 605
SphI G_CATG'C
Cuts at: 0 1563 1833
Size: 1563 270
TatI w'GTAC_w
Cuts at: 0 427 469 1433 1766 1833
Size: 427 42 964 333 67
Fragments arranged by size:
964 427 333 67 42
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 1833
Size: 12 1821
Enzymes that do cut:
Acc65I AclI AcuI AleI AlfI ApoI AseI BaeI
BamHI BanI BbsI BclI BfrBI BglI BmgBI BmtI
BplI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstXI
BstYI Bsu36I CspCI DraI DraIII DrdI EaeI EagI
EarI Eco57MI EcoRV HaeII HincII KpnI MmeI MslI
MspA1I NheI NsiI NspI PflMI PfoI PsiI PstI
PvuII SfcI SmlI SphI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BanII BbeI BbvCI BcgI BciVI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BsaAI BsaBI BsgI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BssHII BssSI
BstEII BstZ17I BtgI BtgZI BtsI ClaI EciI EcoICRI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI Hin4I
HindIII HpaI KasI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NotI NruI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI StyI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI